MS-Fit Search Results



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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D94
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (0 - 100000 Da) selects 107150 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12217 entries.
Pre searches select 12217 entries.

Data Set 1 Results

MS-Fit search selects 326 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score
#/36(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.297e+00812 (33)69.033.387.034.2 27547/5.7HUMAN6-phosphogluconolactonase (6PGL)
213615 (13)16.013.975.190.3 55732/6.7MOUSETyrosine-protein kinase SRM (PTK70)
310625 (13)12.013.925.3178 70223/7.2HUMANHepatocellular carcinoma-associated antigen 66
410265 (13)12.013.935.2119 60705/6.2HUMANExtracellular matrix protein 1 precursor (Secretory component P85)
58285 (13)12.013.923.8136 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
64724 (11)21.011.164.055.2 39158/6.0HUMANActivator 1 40 kDa subunit (Replication factor C 40 kDa subunit) (A1 40 kDa subunit) (RF-C 40 kDa subunit) (RFC40)
72865 (13)11.013.999.2101 86309/9.2MOUSEExostosin-1 (Multiple exostoses protein 1 homolog)
82646 (16)17.016.721.5167 82655/6.4HUMANVesicular-fusion protein NSF (N-ethylmaleimide-sensitive fusion protein) (NEM-sensitive fusion protein)
92185 (13)8.013.92.49113 82556/6.4HUMANHypothetical protein KIAA1034
101834 (11)15.011.142.0173 53269/5.7MOUSELamin B3
81694.x..........xx...x..xxxx.x...x....xx
1670................x.x..x...x.......x..
70453.....x....x......x..........xx......
15261.x..........x......x...x......x.....
45313....x.x...x........x......x.........
88556...............x............xx.....x
22423...................x...x...x..x..x..
16203....x..x..................x.x.xx....
19899.x...x.....x.......x............x...
27664.......x.........x.........x.x......



Detailed Results

1. 12/36 matches (33%).
Acc. #: Species: HUMAN Name: 6-phosphogluconolactonase (6PGL)
Index: MW: 27547 Da pI: 5.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
977.5073977.46614230380(R) (A)
1197.64061197.53638773810(R) (L)
1267.88941267.7738911861970(R) (T)
1365.78331365.6625882362460(K) (L)
1490.97161490.83319357720(R) (W)
1579.03311578.867810541560(R) (E)
1594.99121594.8627811Met-ox 41560(R)FALGLSGGSLVSMLAR(E)
1602.02231601.9015751711850(K) (V)
1768.03591767.87069382960(R) (T)
2299.37702299.1934802152350(R) (L)
2794.80102794.5103104AcetN 1290(-)AAPAPGLISVFSSSQELGAALAQLVAQR(A)
2865.77492865.47731041031280(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 69% (179/258AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/36 matches (13%).
Acc. #: Species: MOUSE Name: Tyrosine-protein kinase SRM (PTK70)
Index: MW: 55732 Da pI: 6.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1351.75461351.6858513123240(K) (A)
1472.87641472.7862614694810(K) (E)
1579.03311578.80151473813951(K) (W)
1768.03591767.8818873964101(K) (S)
2705.42212705.3431293253480(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (80/496AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/36 matches (13%).
Acc. #: Species: HUMAN Name: Hepatocellular carcinoma-associated antigen 66
Index: MW: 70223 Da pI: 7.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1018.43491018.5362-991781841(K) (M)
1092.55541092.5400141Met-ox 3193270(K)TLPTEAMWK(C)
1092.55541092.6094-492192270(K) (N)
1365.78331365.58911425205300(K) (E)
2284.38562284.18658752690(K) (R)
2299.37702299.2459571291490(K) (W)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (75/597AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/36 matches (13%).
Acc. #: Species: HUMAN Name: Extracellular matrix protein 1 precursor (Secretory component P85)
Index: MW: 60705 Da pI: 6.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
977.5073977.4580503613680(K) (Y)
1197.64061197.7207-673343430(R) (E)
1475.88321475.7793704664771(K) (N)
1602.02231601.8903821011150(K) (E)
2369.27292369.1791401862050(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (65/540AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/36 matches (13%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
999.4482999.44572.52902980(K) (V)
999.4482999.4972-493873951(R) (I)
1033.43321033.5166-814844920(R) (M)
1092.55541092.5034486036160(R) (G)
1475.88321475.7494912122230(K) (T)
1475.88321475.7858662002110(R) (W)
1994.15551993.9772892242390(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (81/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 4/36 matches (11%).
Acc. #: Species: HUMAN Name: Activator 1 40 kDa subunit (Replication factor C 40 kDa subunit) (A1 40 kDa subunit) (RF-C 40 kDa subunit) (RFC40)
Index: MW: 39158 Da pI: 6.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1320.74111320.6986322542640(K) (E)
2284.38562284.18798727461(K) (L)
2299.37702299.2630503273471(K) (T)
2865.77492865.5238882813041(K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (76/354AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 5/36 matches (13%).
Acc. #: Species: MOUSE Name: Exostosin-1 (Multiple exostoses protein 1 homolog)
Index: MW: 86309 Da pI: 9.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1475.88321475.66231501Met-ox 6776881(K)QYKETMMGQTSR(A)
1602.02231601.79321433263380(R) (G)
2211.31732211.1232882812990(R) (H)
2369.27292369.2141252192390(K) (D)
2705.42212705.177391pyroGlu 1Met-ox 7027230(R)QSCMNTFASWFGYMPLIHSQMR(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (87/746AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 6/36 matches (16%).
Acc. #: Species: HUMAN Name: Vesicular-fusion protein NSF (N-ethylmaleimide-sensitive fusion protein) (NEM-sensitive fusion protein)
Index: MW: 82655 Da pI: 6.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
999.4482999.4821-341Met-ox 5735810(K)MIGFSETAK(C)
999.4482999.5151-675875941(K) (S)
1046.47241046.5781-1011Met-ox 2722801(R)QIGKMLNAR(E)
1994.15551993.921111811270(R) (D)
2284.38562284.1356109pyroGlu 1Met-ox 3363571(K)QRGSMAGSTGVHDTVVNQLLSK(I)
2284.38562284.18658768871(R) (A)
2369.27292369.26513.33153351(R) (Q)
2546.52182546.37715745671(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (129/744AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 5/36 matches (13%).
Acc. #: Species: HUMAN Name: Hypothetical protein KIAA1034
Index: MW: 82556 Da pI: 6.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
977.5073977.5090-1.71541610(K) (L)
1018.43491018.5104-744111(R) (D)
1133.46971133.4962-237137220(K) (S)
1475.88321475.7793705335431(R) (F)
2569.49642569.3904413263470(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (59/733AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 4/36 matches (11%).
Acc. #: Species: MOUSE Name: Lamin B3
Index: MW: 53269 Da pI: 5.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1046.47241046.5231-4883901(R) (R)
1046.47241046.5305-551121200(K) (S)
1365.78331365.61881214044150(K) (T)
2211.31732211.1257873183380(R) (F)
2299.37702299.13121072682911(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (74/474AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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