MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D95
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 867 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/53(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.049e+00711 (20)50.020.844.448.9 27362/5.4HUMANProteasome activator complex subunit 2 (Proteasome activator 28-beta subunit) (PA28beta) (PA28b) (Activator of multicatalytic protease subunit 2) (11S regulator complex beta subunit) (REG-beta)
21.996e+0049 (16)19.017.048.355.3 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
342187 (13)10.013.211.1139 88931/8.7HUMANCullin homolog 3 (CUL-3)
431888 (15)15.015.12.1264.5 78752/9.5MOUSEG2 and S phase expressed protein 1 (Gtse-1) (B99 protein)
5309810 (18)14.018.9-2.71119 82268/9.0HUMANZinc finger protein 224
628284 (7)15.07.522.8101 35620/9.2HUMANSideroflexin 1
727546 (11)14.011.3-6.96129 54872/8.1HUMANHYPOTHETICAL PROTEIN DJ845O24.3
824785 (9)17.09.46.44169 57453/5.8HUMANAlkaline phosphatase, placental precursor (Regan isozyme)
923695 (9)8.09.411.269.0 87433/8.7HUMANCGMP-DEPENDENT PROTEIN KINASE 2 (CGK 2) (CGKII) (TYPE II CGMP-DEPENDENT PROTEIN KINASE)
1023445 (9)17.09.4-25.9116 57316/5.8HUMANAlkaline phosphatase, placental-like precursor (Nagao isozyme) (Germ-cell alkaline phosphatase) (PLAP-like) (ALP-1)
11555....x......x.x..x......x..x.x....x..x..x..........x..
45313................x.xx.xx...x................xx....x...
98109.....x.........x.........x....x......x.x..x..........
10858...x.............x........xx...........x.x......x..x.
65938....x.x....xx.x...x..........x.x.....x............x..
3426.........................x...x..x................x...
41756........x.xx..........................x.x..........x.
6102..........x.....................x.......x.......x...x
33696...........x..............x.x...................xx...
32498.......x........................x...............x..xx


Detailed Results

1. 11/53 matches (20%).
Acc. #: Species: HUMAN Name: Proteasome activator complex subunit 2 (Proteasome activator 28-beta subunit) (PA28beta) (PA28b) (Activator of multicatalytic protease subunit 2) (11S regulator complex beta subunit) (REG-beta)
Index: MW: 27362 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
905.5192905.5209-1.915211(R) (Q)
1023.50001023.4747251952030(R) (A)
1035.49881035.49335.391990(K) (V)
1066.51141066.4628461Met-ox 1811880(K)ETHVMDYR(A)
1534.80371534.7389421321450(K) (V)
1669.88561669.786259pyroGlu 22340(R)QNLFQEAEEFLYR(F)
1686.91521686.81286122340(R) (F)
1728.96661728.8669581892031(R) (A)
1809.24011809.1254631001150(K) (E)
1898.09181897.9700642112260(R) (I)
2531.54022531.37216640610(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 50% (120/239AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/53 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1066.51141066.5169-5.22702771(K) (T)
1157.53281157.5149151Met-ox 4644720(R)DYQELMNTK(L)
1179.66651179.6010563773860(K) (H)
1383.76301383.6909521861970(K) (V)
1475.83671475.7494592122230(K) (T)
1475.83671475.7858342002110(R) (W)
1669.88561669.8107451Met-ox 2903041(K)DVDGAYMTKVDLQAK(L)
1994.09941993.9772612242390(R) (R)
2150.26692150.0783882242401(R) (R)
2384.13662383.9524775195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (126/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/53 matches (13%).
Acc. #: Species: HUMAN Name: Cullin homolog 3 (CUL-3)
Index: MW: 88931 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
919.3828919.4790-1055305360(R) (R)
1056.44551056.4858-381211280(R) (V)
1642.87931642.8593123553671(R) (Q)
1700.93751700.8715392752890(K) (N)
1839.06061838.9488615225361(K) (R)
1898.09181897.88201116116250(K) (E)
1941.06761941.0704-1.46266421(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (83/768AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/53 matches (15%).
Acc. #: Species: MOUSE Name: G2 and S phase expressed protein 1 (Gtse-1) (B99 protein)
Index: MW: 78752 Da pI: 9.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
886.4048886.39807.74334390(R) (T)
1074.52961074.5618-301Met-ox 5135220(R)MSVLPTPASR(R)
1669.88561669.8509213783931(R) (V)
1683.89811683.9038-3.45235371(R) (A)
1898.09181897.9911531171321(K) (E)
1920.07781920.0191313944100(K) (G)
2369.21542369.2618-2080991(K) (R)
2705.35272705.4669-425245481(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (112/741AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/53 matches (18%).
Acc. #: Species: HUMAN Name: Zinc finger protein 224
Index: MW: 82268 Da pI: 9.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
905.5192905.4957262452520(R) (L)
927.3940927.4325-416046110(K) (L)
927.3940927.4358-455175241(K) (F)
1023.50001023.49336.635420(R) (N)
1026.40221026.5155-1106656720(R) (V)
1051.56031051.539720pyroGlu 3273351(R)QRSALNSHR(M)
1157.53281157.5777-391841920(K) (I)
1691.82851691.8804-311841961(K) (V)
1707.86481707.8423136656781(R) (T)
1839.06061838.9448631Met-ox 3033171(R)LNRHSMVHTAEKPFR(C)
2531.54022531.26561084935131(R) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (104/707AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 4/53 matches (7%).
Acc. #: Species: HUMAN Name: Sideroflexin 1
Index: MW: 35620 Da pI: 9.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1642.87931642.73898573860(K) (M)
1691.82851691.8757-28pyroGlu 56700(R)QGIVPPGLTENELWR(A)
1708.89051708.9022-6.956700(R) (A)
2384.13662384.040440931120(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (49/322AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 6/53 matches (11%).
Acc. #: Species: HUMAN Name: HYPOTHETICAL PROTEIN DJ845O24.3
Index: MW: 54872 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
939.3866939.4643-831Met-ox 2302361(K)EMKTLCK(L)
1007.44031007.5018-614354420(R) (E)
1023.50001023.49673.31Met-ox 4354420(R)AELMCTLR(E)
1852.03261851.8562951161310(R) (R)
1912.09361912.0366301411561(R) (E)
2705.35272705.4238-262012220(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (69/474AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 5/53 matches (9%).
Acc. #: Species: HUMAN Name: Alkaline phosphatase, placental precursor (Regan isozyme)
Index: MW: 57453 Da pI: 5.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1007.44031007.5526-1113883980(R) (A)
1708.89051708.8519231681830(R) (N)
1912.09361911.86801182Met-ox 2983140(R)DSTLDPSLMEMTEAALR(L)
2369.21542369.1373334384590(R) (G)
2717.24252717.3238-301972210(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (91/530AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 5/53 matches (9%).
Acc. #: Species: HUMAN Name: CGMP-DEPENDENT PROTEIN KINASE 2 (CGK 2) (CGKII) (TYPE II CGMP-DEPENDENT PROTEIN KINASE)
Index: MW: 87433 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1023.50001023.49336.64744820(K) (C)
1669.88561669.864912pyroGlu 61750(K)QTVAIAELTEELQNK(C)
1686.91521686.89141461750(K) (C)
2369.21542369.0728601791971(K) (Q)
2384.13662384.2250-374444660(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (66/762AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 5/53 matches (9%).
Acc. #: Species: HUMAN Name: Alkaline phosphatase, placental-like precursor (Nagao isozyme) (Germ-cell alkaline phosphatase) (PLAP-like) (ALP-1)
Index: MW: 57316 Da pI: 5.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
929.3904929.5056-12434430(R) (K)
1708.89051708.8519231701850(R) (N)
2369.21542369.21410.5314330(R) (Q)
2705.35272705.34901.42Met-ox 3003231(R)DSTLDPSLMEMTEAALLLLSRNPR(G)
2717.24252717.3238-301992230(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (95/532AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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