MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D96
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1620 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/77(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	7.885e+004	14 (18)	42.0	18.2	14.1	66.7		31351/5.4		HUMAN	F-actin capping protein beta subunit (CAPZ beta)
2	1.347e+004	12 (15)	12.0	15.6	-14.5	77.2		99104/5.5		HUMAN	Cone cGMP-specific 3',5'-cyclic phosphodiesterase alpha'-subunit
3	1.027e+004	9 (11)	13.0	11.7	7.21	85.6		69900/6.3		HUMAN	Baculoviral IAP repeat-containing protein 3 (Inhibitor of apoptosis protein 2) (HIAP2) (HIAP-2) (C-IAP1) (TNFR2-TRAF signaling complex protein 2) (IAP homolog B)
4	7394	12 (15)	39.0	15.6	20.2	60.0		31346/5.5		MOUSE	F-actin capping protein beta subunit (CAPZ beta)
5	5864	8 (10)	9.0	10.4	-4.35	88.9		90848/6.1		MOUSE	Plasminogen precursor [Contains: Angiostatin]

57695	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	x	x	.	x	x	.	.	x	.	x
13125	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	x	.	.	.	.	.	.	x	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.
18491	.	.	.	.	.	x	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.
10624	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	x	x	.	x	x	.	.	x	.	x
24423	.	x	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.

Detailed Results

1. 14/77 matches (18%).
Acc. #: Species: HUMAN Name: F-actin capping protein beta subunit (CAPZ beta)
Index: MW: 31351 Da pI: 5.4

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
952.4868	952.5468	-63		16	23	0	(R) (N)
973.4660	973.4631	3.0	pyroGlu	270	277	0	(R)QIYIQPDN(-)
990.4412	990.4896	-49		270	277	0	(R) (-)
993.4689	993.4563	13	1Met-ox	216	223	0	(R)LVEDMENK(I)
1015.5961	1015.5900	6.0		236	244	1	(K) (S)
1108.6915	1108.6479	39		15	23	1	(R) (N)
1171.6426	1171.5999	36		226	235	0	(R) (T)
1245.6195	1245.5574	50		58	66	0	(K) (D)
1620.7694	1620.7362	21	AcetN	1	14	0	(-)SDQQLDCALDLMR(R)
1636.7891	1636.7311	35	AcetN 1Met-ox	1	14	0	(-)SDQQLDCALDLMR(R)
1696.8831	1696.8295	32		95	108	1	(R) (D)
1699.8661	1699.8114	32		146	159	0	(K) (S)
1940.9942	1940.9904	1.9		144	159	1	(K) (S)
2234.1076	2234.0188	40	1Met-ox	73	92	1	(R)SPWSNKYDPPLEDGAMPSAR(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 42% (117/277AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/77 matches (15%).
Acc. #: Species: HUMAN Name: Cone cGMP-specific 3',5'-cyclic phosphodiesterase alpha'-subunit
Index: MW: 99104 Da pI: 5.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
964.4593 964.4264 34 pyroGlu 208 215 0 (K)QDEEVFSK(Y)
981.4325 981.4529 -21 208 215 0 (K) (Y)
983.5120 983.5137 -1.8 548 554 1 (R) (G)
1001.4282 1001.4371 -8.9 96 102 0 (R) (S)
1011.4665 1011.4635 3.0 814 822 0 (K) (M)
1021.4617 1021.4074 53 840 850 0 (K) (K)
1092.5858 1092.5611 23 1Met-ox 823 831 1 (K)MKVIEEEAK(K)
1133.4706 1133.5479 -68 123 132 0 (K) (E)
1140.5235 1140.5975 -65 277 286 0 (R) (E)
1245.6195 1245.6744 -44 pyroGlu 260 269 1 (R)QFHKALYTVR(S)
1302.7254 1302.7785 -41 28 38 1 (K) (N)
1570.8251 1570.8845 -38 320 332 1 (K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (105/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/77 matches (11%).
Acc. #: Species: HUMAN Name: Baculoviral IAP repeat-containing protein 3 (Inhibitor of apoptosis protein 2) (HIAP2) (HIAP-2) (C-IAP1) (TNFR2-TRAF signaling complex protein 2) (IAP homolog B)
Index: MW: 69900 Da pI: 6.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
963.4453 963.4747 -31 165 173 0 (R) (T)
968.5662 968.4941 75 530 537 0 (K) (N)
974.4740 974.4842 -10 1Met-ox 1 8 1 (-)MHKTASQR(L)
997.4605 997.4599 0.59 218 226 0 (R) (L)
1024.4519 1024.4886 -36 398 406 0 (K) (D)
1040.4892 1040.4835 5.4 1Met-ox 398 406 0 (K)SALEMGFNR(D)
1122.5791 1122.6271 -43 95 104 1 (K) (Q)
1234.6372 1234.5744 51 70 80 0 (R) (V)
1940.9942 1940.8911 53 20 36 0 (K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (81/618AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 12/77 matches (15%).
Acc. #: Species: MOUSE Name: F-actin capping protein beta subunit (CAPZ beta)
Index: MW: 31346 Da pI: 5.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
952.4868 952.5468 -63 16 23 0 (R) (N)
993.4689 993.4563 13 1Met-ox 216 223 0 (R)LVEDMENK(I)
1015.5961 1015.5900 6.0 236 244 1 (K) (S)
1108.6915 1108.6479 39 15 23 1 (R) (N)
1171.6426 1171.5999 36 226 235 0 (R) (T)
1245.6195 1245.5574 50 58 66 0 (K) (D)
1620.7694 1620.7362 21 AcetN 1 14 0 (-)SDQQLDCALDLMR(R)
1636.7891 1636.7311 35 AcetN 1Met-ox 1 14 0 (-)SDQQLDCALDLMR(R)
1696.8831 1696.8295 32 95 108 1 (R) (D)
1699.8661 1699.8114 32 146 159 0 (K) (S)
1940.9942 1940.9904 1.9 144 159 1 (K) (S)
2234.1076 2234.0188 40 1Met-ox 73 92 1 (R)SPWSNKYDPPLEDGAMPSAR(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 39% (109/277AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/77 matches (10%).
Acc. #: Species: MOUSE Name: Plasminogen precursor [Contains: Angiostatin]
Index: MW: 90848 Da pI: 6.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
952.4868 952.4951 -8.7 387 396 0 (R) (K)
964.4593 964.4892 -31 801 807 0 (R) (E)
968.5662 968.5529 14 659 666 0 (K) (D)
992.4477 992.5199 -73 118 126 1 (R) (W)
1006.4267 1006.4668 -40 310 317 0 (R) (N)
1040.4892 1040.4914 -2.1 299 306 0 (R) (H)
1111.5554 1111.4842 64 318 325 0 (K) (N)
1871.9821 1871.9036 42 262 277 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (74/812AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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