MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D99
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (0 - 100000 Da) selects 107150 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12217 entries.
Pre searches select 12217 entries.

Data Set 1 Results

MS-Fit search selects 1336 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/63(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.586e+00919 (30)58.030.2-29.650.7 36376/5.6HUMANAnnexin III (Lipocortin III) (Placental anticoagulant protein III) (PAP-III) (35-alpha calcimedin) (Inositol 1,2-cyclic phosphate 2-phosphohydrolase)
23.255e+00510 (15)24.015.9-19.958.2 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
31.345e+0048 (12)11.012.7-48.970.1 87151/5.1MOUSECell-cycle autoantigen SG2NA (S/G2 antigen)
41.037e+0049 (14)18.014.3-7.5965.4 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
5916910 (15)18.015.9-25.154.1 82655/6.4HUMANVesicular-fusion protein NSF (N-ethylmaleimide-sensitive fusion protein) (NEM-sensitive fusion protein)
663297 (11)20.011.1-31.642.1 36371/5.3MOUSEAnnexin III (Lipocortin III) (Placental anticoagulant protein III) (PAP-III) (35-alpha calcimedin)
753445 (7)17.07.9-37.677.8 51364/4.9MOUSEGA binding protein alpha chain (GABP-alpha subunit)
8480213 (20)19.020.6-46.575.5 86691/6.0MOUSEInhibitor of nuclear factor kappa B kinase beta subunit (I-kappa-B-kinase beta) (IkBKB) (IKK-beta) (IKK-B) (I-kappa-B kinase 2) (IKK2) (Nuclear factor NF-kappa-B inhibitor kinase beta) (NFKBIKB)
946909 (14)11.014.39.70114 76765/6.6HUMANProtein kinase C, alpha type (PKC-alpha) (PKC-A)
1046849 (14)11.014.39.70114 76853/6.7MOUSEProtein kinase C, alpha type (PKC-alpha) (PKC-A)
34825.....xx....x....x.xxx..x...x.x..x.x..xx.....x..xx....x......x..
45313...............x...x.x...x..x......x...x.....x..........x..x...
51055...xx.....................x.....x...x....x............x...x....
97443...........x......x........x..xxx...............x..x.........x.
16203..........x...xx...x........................x........xx.xxx....
94566.....xx.........x..x.................x......x.......x..........
98486..............................x......x.......x........x.....x..
103128........x...x.........x.........x...xxx.x...x........xx.....x.x
4226x...x.x.....x........x........xxx.................x............
91123x...x.x.....x........x........xxx.................x............


Detailed Results

1. 19/63 matches (30%).
Acc. #: Species: HUMAN Name: Annexin III (Lipocortin III) (Placental anticoagulant protein III) (PAP-III) (35-alpha calcimedin) (Inositol 1,2-cyclic phosphate 2-phosphohydrolase)
Index: MW: 36376 Da pI: 5.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
929.4729929.43693959660(K) (E)
929.4729929.5420-741551630(K) (R)
943.4088943.4525-462112170(K) (N)
967.4798967.5114-33AcetN 190(-)ASIWVGHR(G)
1018.49361018.5322-382492570(R) (L)
1065.50721065.5767-651972040(K) (S)
1073.55211073.5843-302802880(R) (T)
1075.56301075.6185-5240480(K) (S)
1091.56911091.6135-411Met-ox 40480(K)MLISILTER(S)
1222.57501222.6068-262642740(K) (I)
1301.61241301.6530-312943040(K) (S)
1350.63291350.6582-191271370(K) (K)
1429.71821429.7480-212933041(K) (S)
1585.67931585.6982-121391530(K) (K)
1673.83901673.8710-191051200(K) (T)
1713.76051713.7931-191391541(K) (A)
1713.76051713.7931-191381531(K) (K)
1781.79921781.8234-1414290(R) (A)
1791.81591791.9526-761Met-ox 33481(R)GIGTDEKMLISILTER(S)
1896.90421896.9166-6.51902041(R) (S)
2567.24982567.2968-181Met-ox 74970(K)GDLSGHFEHLMVALVTPPAVFDAK(Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 58% (189/323AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/63 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
999.4693999.4457242902980(K) (V)
999.4693999.4972-283873951(R) (I)
1073.55211073.5955-404084161(R) (K)
1087.55481087.5285244121(R) (S)
1179.55931179.6010-353773860(K) (H)
1300.50511300.5302-192582670(K) (N)
1475.71991475.7494-202122230(K) (T)
1475.71991475.7858-452002110(R) (W)
1687.85641687.9707-6866821(R) (G)
1716.78131716.8517-414184320(K) (G)
1993.96771993.9772-4.82242390(R) (R)
2383.98712383.9524155195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (159/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/63 matches (12%).
Acc. #: Species: MOUSE Name: Cell-cycle autoantigen SG2NA (S/G2 antigen)
Index: MW: 87151 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
914.4086914.4736-716116170(R) (L)
926.3844926.4484-6984900(R) (A)
1221.53991221.6479-887817930(K) (V)
1350.63291350.6919-444734831(K) (A)
1566.71021566.8532-917817961(K) (-)
1701.80541701.8659-362022180(R) (N)
1912.88471912.8565153033200(K) (S)
1912.88471912.9656-421811960(R) (S)
2284.12722284.1573-131811991(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (95/796AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/63 matches (14%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
967.4798967.4525281841900(K) (K)
1065.50721065.49996.81541620(K) (L)
1222.57501222.6102-291Met-ox 3173261(K)DLTKTLNDMR(Q)
1307.64201307.6782-282402491(R) (Q)
1323.61151323.6731-471Met-ox 2402491(R)IKFEMEQNLR(Q)
1350.63291350.6878-414724861(K) (G)
1791.81591791.7283494905120(R) (G)
1851.89441851.9275-183213351(K) (N)
2705.18672705.16179.363940(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (115/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/63 matches (15%).
Acc. #: Species: HUMAN Name: Vesicular-fusion protein NSF (N-ethylmaleimide-sensitive fusion protein) (NEM-sensitive fusion protein)
Index: MW: 82655 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
965.4064965.4475-431Met-ox 4474550(R)AAQSTAMNR(H)
983.4683983.4872-195735810(K) (C)
999.4693999.4821-131Met-ox 5735810(K)MIGFSETAK(C)
999.4693999.5151-465875941(K) (S)
1073.55211073.6319-746927011(R) (K)
1713.76051713.8192-341Met-ox 5675811(K)ICSPDKMIGFSETAK(C)
1896.90421896.9716-362392540(R) (G)
1912.88471912.9665-431Met-ox 2392540(R)VFPPEIVEQMGCIHVK(G)
1993.96771993.92112311270(R) (D)
2142.09102142.0649122Met-ox 7097251(K)KLLMLIEMSLQMDPEYR(V)
2284.12722284.1356-3.6pyroGlu 1Met-ox 3363571(K)QRGSMAGSTGVHDTVVNQLLSK(I)
2284.12722284.1865-2668871(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (134/744AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 7/63 matches (11%).
Acc. #: Species: MOUSE Name: Annexin III (Lipocortin III) (Placental anticoagulant protein III) (PAP-III) (35-alpha calcimedin)
Index: MW: 36371 Da pI: 5.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
929.4729929.5420-741551630(K) (R)
943.4088943.4525-462112170(K) (N)
1018.49361018.5322-382492570(R) (L)
1073.55211073.5843-302802880(R) (H)
1585.67931585.6982-121391530(K) (K)
1713.76051713.7931-191391541(K) (A)
1713.76051713.7931-191381531(K) (K)
1882.87481882.9009-141902041(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (66/323AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 5/63 matches (7%).
Acc. #: Species: MOUSE Name: GA binding protein alpha chain (GABP-alpha subunit)
Index: MW: 51364 Da pI: 4.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1307.64201307.6959-414054160(K) (L)
1585.67931585.8338-971761881(K) (W)
1716.78131716.8404-341511650(K) (W)
1912.88471912.9326-252042200(K) (E)
2567.24982567.22341059790(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (78/454AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 13/63 matches (20%).
Acc. #: Species: MOUSE Name: Inhibitor of nuclear factor kappa B kinase beta subunit (I-kappa-B-kinase beta) (IkBKB) (IKK-beta) (IKK-B) (I-kappa-B kinase 2) (IKK2) (Nuclear factor NF-kappa-B inhibitor kinase beta) (NFKBIKB)
Index: MW: 86691 Da pI: 6.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
954.4420954.5009-62pyroGlu 48551(R)QELSPKNR(N)
971.4416971.5274-8848551(R) (N)
1090.49811090.6009-9421310(R) (W)
1350.63291350.5782405165260(R) (E)
1350.63291350.7395-791351441(R) (D)
1566.71021566.6818181Met-ox 5805921(R)DQRTEGDSQEMVR(L)
1585.67931585.7644-547057180(K) (L)
1673.83901673.8598-122392540(K) (F)
1696.78041696.9162-804834961(K) (Y)
1713.76051713.8594-587047181(K) (L)
1896.90421896.9981-501Met-ox 2732861(R)LEKWLQLMLMWHPR(Q)
1912.88471912.9868-53pyroGlu 2372541(R)QKSEVDIVVSEDLNGAVK(F)
1912.88471912.9930-572Met-ox 2732861(R)LEKWLQLMLMWHPR(Q)
2567.24982567.3483-3821441(R) (Q)
2717.09372717.1789-311Met-ox 7297491(K)EQDRSFTTLDWSWLQMEDEER(C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (148/757AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 9/63 matches (14%).
Acc. #: Species: HUMAN Name: Protein kinase C, alpha type (PKC-alpha) (PKC-A)
Index: MW: 76765 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
820.3923820.4239-395645700(K) (G)
926.3844926.4332-53pyroGlu 3263330(K)QPSNNLDR(V)
943.4088943.4597-543263330(K) (V)
971.4416971.43308.81Met-ox 4794860(K)IADFGMCK(E)
1087.55481087.502049921011(K) (H)
1307.64201307.5183951321410(K) (Q)
1323.61151323.5132741Met-ox 1321410(K)CDTCDMNVHK(Q)
1350.63291350.7125-595645751(K) (H)
1838.90991838.83184277911(K) (G)
1838.90991838.85043263760(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (77/672AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 9/63 matches (14%).
Acc. #: Species: MOUSE Name: Protein kinase C, alpha type (PKC-alpha) (PKC-A)
Index: MW: 76853 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
820.3923820.4239-395645700(K) (G)
926.3844926.4332-53pyroGlu 3263330(K)QPSNNLDR(V)
943.4088943.4597-543263330(K) (V)
971.4416971.43308.81Met-ox 4794860(K)IADFGMCK(E)
1087.55481087.502049921011(K) (H)
1307.64201307.5183951321410(K) (Q)
1323.61151323.5132741Met-ox 1321410(K)CDTCDMNVHK(Q)
1350.63291350.7125-595645751(K) (Q)
1838.90991838.83184277911(K) (G)
1838.90991838.85043263760(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (77/672AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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