MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D100
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (0 - 100000 Da) selects 107150 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12217 entries.
Pre searches select 12217 entries.

Data Set 1 Results

MS-Fit search selects 2486 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/98(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.818e+00917 (17)34.017.320.346.3 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
24.407e+0049 (9)20.09.2-8.0876.7 72259/5.9MOUSEHistidine ammonia-lyase (Histidase)
32.684e+0049 (9)21.09.212.651.3 59621/5.4HUMANT-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
42.362e+0049 (9)15.09.26.3551.8 96968/6.0MOUSEOxysterol binding protein-related protein 3 (OSBP-related protein 3) (ORP-3)
51.705e+00414 (14)15.014.3-1.4181.7 93868/7.0HUMANHYPOTHETICAL PROTEIN KIAA0184
61.010e+0049 (9)14.09.215.454.7 79542/9.4HUMANBromodomain-containing protein 3 (RING3-like protein)
7950010 (10)13.010.214.159.9 94506/5.6HUMANOsmotic stress protein 94 (Heat shock 70-related protein APG-1)
8936210 (10)13.010.29.9172.9 90601/6.3HUMANProtein-tyrosine phosphatase alpha precursor (R-PTP-alpha)
983658 (8)18.08.213.568.2 55677/7.7HUMANUTP--glucose-1-phosphate uridylyltransferase 2 (UDP-glucose pyrophosphorylase 2) (UDPGP 2) (UGPase 2)
1075569 (9)12.09.221.676.6 89615/8.6HUMANTumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
45313....x.............x.....x.....x......xxx.......xx.x........xx.x.x......x.x.................x......
17841...............x............x................x........x...............x.x.........xx.x............
61980.......................x....x....x......x..x...........x............x....................x.x......
10233......................................x.............x....x............x.x....x..x.....xx..........
109007....x..x........x....x........x.xx.............x......xx.........x........x...x...........x.......
2755....x................................x......x..x...xx.x.x.....................................x...
66994...............x.x.........x....x.......x.........x.......x.................x..x............x.....
70997........................x...x....x.....x............x..x......................x.x..x.......x......
87699.........x..........x...x..........x.................x........x.......x.....................x.....
42717...........x..................x...................xx..........x.x.x.x....x........................


Detailed Results

1. 17/98 matches (17%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
832.4870832.4892-2.775820(K) (G)
973.5354973.53183.63964030(K) (V)
1073.60791073.5955124084161(R) (K)
1179.61341179.6010113773860(K) (H)
1277.74621277.7105284734830(K) (T)
1300.60141300.5302552582670(K) (N)
1302.70741302.70184.43934031(R) (V)
1302.70741302.7157-6.33443550(R) (A)
1383.73891383.6909351861970(K) (V)
1421.71791421.7389-152772881(K) (K)
1475.79391475.7494302122230(K) (T)
1475.79391475.78585.52002110(R) (W)
1669.93481669.8107741Met-ox 2903041(K)DVDGAYMTKVDLQAK(L)
1688.02801687.97073466821(R) (G)
1699.88721699.825137pyroGlu 4184320(K)QISNLQQSISDAEQR(G)
1716.88171716.8517184184320(K) (G)
1941.97441941.9882-7.14444601(K) (L)
1994.03231993.9772282242390(R) (R)
2384.05572383.9524435195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 34% (222/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/98 matches (9%).
Acc. #: Species: MOUSE Name: Histidine ammonia-lyase (Histidase)
Index: MW: 72259 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
916.5311916.5355-4.81511580(R) (E)
1158.60021158.6206-18191(-) (G)
1373.66961373.65241310210(R) (L)
1514.75951514.8542-631461581(K) (E)
1941.02441941.0446-104524690(K) (R)
1968.92941968.9741-232322480(K) (G)
2097.02652097.1457-574524701(K) (I)
2211.18612211.0603571Met-ox 4164350(K)DIITTELNSATDNPMVFASR(G)
2233.17472233.0750453683861(R) (F)
2233.17472233.2192-202492720(K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (133/657AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/98 matches (9%).
Acc. #: Species: HUMAN Name: T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
Index: MW: 59621 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1065.53271065.5217103914000(R) (V)
1158.60021158.59078.24674760(K) (N)
1232.63141232.6098182973070(K) (Y)
1307.71831307.63846121310(K) (N)
1350.73271350.7568-183083181(K) (W)
1546.82161546.80121332441(R) (T)
1851.97171851.90233738541(K) (M)
2298.28022298.26446.955741(K) (E)
2384.05572384.1114-232612811(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (117/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/98 matches (9%).
Acc. #: Species: MOUSE Name: Oxysterol binding protein-related protein 3 (OSBP-related protein 3) (ORP-3)
Index: MW: 96968 Da pI: 6.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1300.60141300.6174-12pyroGlu 31410(R)QDSWEVVEGLR(G)
1498.75931498.7800-146546660(K) (W)
1628.84851628.8504-1.2941080(K) (K)
1941.02441940.9354468258411(R) (K)
1941.02441940.9354468268421(K) (D)
1968.92941968.9588-155275421(R) (I)
2052.06192052.0224195685850(R) (Q)
2083.06352083.1188-274825010(R) (N)
2239.21352239.2199-2.84815011(R) (N)
2283.25712283.2019241Met-ox 5075261(K)DLSKVAMPVELNEPLNTLQR(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (132/855AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 14/98 matches (14%).
Acc. #: Species: HUMAN Name: HYPOTHETICAL PROTEIN KIAA0184
Index: MW: 93868 Da pI: 7.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
832.4870832.4602323213280(R) (G)
840.4467840.4626-198418461(K) (D)
918.4365918.4831-515615680(K) (T)
1045.58221045.5828-0.593213301(R) (L)
1179.61341179.5832264734810(K) (Q)
1218.61351218.5723344594690(K) (S)
1232.63141232.6785-381Met-ox 1001101(K)LDGLMVTGVRR(H)
1383.73891383.6504642632740(R) (K)
1514.75951514.7742-9.74144260(R) (K)
1546.82161546.7834254594721(K) (L)
1765.80391765.9158-63pyroGlu 1Met-ox 2372540(K)QPEVGPASMIVGNLVAGK(R)
2006.15512006.074540pyroGlu 2352541(R)QKQPEVGPASMIVGNLVAGK(R)
2067.03252067.02195.12562741(R) (K)
2343.09292343.2416-631Met-ox 3240(K)LSVLTVQDVGQVMPGANVCVVK(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (132/863AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 9/98 matches (9%).
Acc. #: Species: HUMAN Name: Bromodomain-containing protein 3 (RING3-like protein)
Index: MW: 79542 Da pI: 9.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
832.4870832.4416555135191(K) (K)
1277.74621277.7806-275926021(K) (E)
1353.82051353.78942368781(K) (N)
1383.73891383.7095214044141(K) (M)
1493.78561493.7447272462600(K) (S)
1498.75931498.7113323904020(K) (K)
1514.75951514.7062351Met-ox 3904020(K)YNPPDHEVVAMAR(K)
1564.79881564.8157-1136480(K) (T)
2705.28082705.3279-174154411(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (108/726AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 10/98 matches (10%).
Acc. #: Species: HUMAN Name: Osmotic stress protein 94 (Heat shock 70-related protein APG-1)
Index: MW: 94506 Da pI: 5.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
916.5311916.52161054610(K) (N)
964.4922964.4926-0.466846910(K) (Y)
1123.45611123.4690-111Met-ox 5205281(K)NENKDNMDK(M)
1218.61351218.5577466927000(K) (A)
1307.71831307.6959171861960(K) (N)
1475.79391475.7342403613740(K) (G)
1650.88711650.9219-212202341(K) (N)
2009.12622009.10729.53894051(K) (W)
2068.07192068.1119-193914071(R) (T)
2550.40012550.222070pyroGlu 5916121(R)QLGQDLLNSYIENEGKMIMQDK(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (115/839AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 10/98 matches (10%).
Acc. #: Species: HUMAN Name: Protein-tyrosine phosphatase alpha precursor (R-PTP-alpha)
Index: MW: 90601 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1073.60791073.5777285525611(R) (N)
1073.60791073.6108-2.73083171(K) (E)
1158.60021158.6370-3234440(R) (E)
1232.63141232.62445.75505601(K) (K)
1302.70741302.70184.46756851(R) (S)
1498.75931498.6783541Met-ox 3833940(K)FCIQQVGDMTNR(K)
1546.82161546.8845-417527651(R) (S)
2067.03252066.9713304494660(R) (K)
2083.06352082.9662471Met-ox 4494660(R)TGTFVVIDAMLDMMHTER(K)
2211.18612211.0612571Met-ox 4494671(R)TGTFVVIDAMLDMMHTERK(V)
2384.05572384.1531-417697860(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (107/802AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 8/98 matches (8%).
Acc. #: Species: HUMAN Name: UTP--glucose-1-phosphate uridylyltransferase 2 (UDP-glucose pyrophosphorylase 2) (UDPGP 2) (UGPase 2)
Index: MW: 55677 Da pI: 7.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
850.4800850.44234443491(K) (L)
850.4800850.44234442481(K) (K)
1029.55231029.5985-454124200(R) (L)
1073.60791073.6206-1215231(R) (E)
1256.72081256.68502885960(R) (L)
1513.79721513.8015-2.84214331(K) (R)
1699.88721699.86551367800(K) (I)
1941.02441941.0446-1067821(K) (A)
2550.40012550.2438614354570(R) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (90/497AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 9/98 matches (9%).
Acc. #: Species: HUMAN Name: Tumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
Index: MW: 89615 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
864.4939864.4579422812871(R) (V)
1179.61341179.5581476776850(K) (F)
1475.79391475.6841741Met-ox 2882990(K)VHFLTDPENEMK(E)
1493.78561493.6921636226320(K) (E)
1699.88721699.9343-2867811(K) (K)
1716.88171716.8267322883011(K) (L)
1791.81481791.8601-256776901(K) (R)
1851.97171851.9751-1.84214390(K) (G)
1994.03231994.0500-8.925410(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (97/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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