MS-Fit Search Results



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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D104
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (0 - 100000 Da) selects 107150 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12217 entries.
Pre searches select 12217 entries.

Data Set 1 Results

MS-Fit search selects 1138 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score
#/53(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
13.132e+01118 (33)66.034.045.227.0 32660/5.5HUMANInorganic pyrophosphatase (Pyrophosphate phospho-hydrolase) (PPase)
24.816e+0059 (16)20.017.040.159.0 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
32.645e+0047 (13)15.013.262.772.1 77888/8.7HUMANZinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
41.673e+0046 (11)14.011.370.3102 78092/9.2HUMANRING finger protein 6
51.380e+00410 (18)12.018.946.8104 97093/6.6HUMANGlycogen phosphorylase, muscle form (Myophosphorylase)
6968811 (20)16.020.833.1112 97287/6.7MOUSEGlycogen phosphorylase, muscle form (Myophosphorylase)
788588 (15)12.015.137.881.3 89615/8.6HUMANTumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
866975 (9)14.09.424.458.2 42321/8.2HUMANHypothetical protein PP2447
961687 (13)16.013.2-7.49102 54912/8.6HUMANDNA polymerase gamma subunit 2, mitochondrial precursor (Mitochondrial DNA polymerase accessory subunit) (PolG-beta) (MtPolB) (DNA polymerase gamma accessory 55 kDa subunit) (p55)
10500612 (22)23.022.630.992.7 74210/6.5MOUSELamin A
105562.x...x.x..x.....x.....x..x.x......x..x.x.xx.x..x..xxx
45313......x....x..x...x....x..x...x............x.....x...
46452........x.................x....x.....x.......xx.x....
98309........x..........x.....x.............x.....x....x..
54458.....x....x..x.........x.x...x.x.x....x....x.........
18650.....x...xx.......x..x.x.x...x.x.x.........x.........
42717...........x......x.......x...x....x....xx.x.........
86008....................x...x...x...........x..........x.
41526.x..............x....x.........x.....x......x....x...
14097.........x.......xx.xx.x..x.x...x...x..........x.x...



Detailed Results

1. 18/53 matches (33%).
Acc. #: Species: HUMAN Name: Inorganic pyrophosphatase (Pyrophosphate phospho-hydrolase) (PPase)
Index: MW: 32660 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
954.4396954.416924AcetN 190(-)SGFSTEER(A)
1053.57581053.53693710180(R) (V)
1114.62791114.59373180880(R) (G)
1177.67301177.6469222122211(K) (S)
1327.73941327.68334242520(K) (W)
1685.87341685.7879512392530(K) (C)
1694.97901694.8906521781910(R) (R)
1701.86751701.7828501Met-ox 2392530(K)GISCMNTTLSESPFK(C)
1790.00091789.9046541401550(K) (V)
1805.98341805.8995461Met-ox 1401550(K)VLGILAMIDEGETDWK(V)
1851.04241850.9917271781921(R) (Y)
1851.04241850.9917271771911(K) (R)
1864.03131863.92815526410(K) (D)
1939.02831938.9238541952110(K) (D)
2176.00372175.9109431101280(K) (V)
2230.21342230.0821591932111(R) (D)
2444.34522444.2058571561771(K) (L)
2461.27682461.121363891090(K) (H)
3172.79733172.59306426521(K) (W)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 66% (191/289AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/53 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1073.58241073.5955-124084161(R) (K)
1179.64111179.6010343773860(K) (H)
1300.64411300.5302882582670(K) (N)
1475.82091475.7494482122230(K) (T)
1475.82091475.7858242002110(R) (W)
1687.99761687.97071666821(R) (G)
1699.86551699.825124pyroGlu 4184320(K)QISNLQQSISDAEQR(G)
1716.93501716.8517494184320(K) (G)
1994.08621993.9772552242390(R) (R)
2384.13542383.9524775195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (132/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/53 matches (13%).
Acc. #: Species: HUMAN Name: Zinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
Index: MW: 77888 Da pI: 8.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1156.59441156.5243612802881(K) (L)
1699.86551699.8815-9.42582701(K) (I)
1726.96571726.8764522072210(R) (I)
1805.98341805.8315844905040(R) (A)
2211.21732211.0756641431610(K) (T)
2225.22572225.0304886066240(R) (K)
2284.29972284.07151004714891(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (109/686AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/53 matches (11%).
Acc. #: Species: HUMAN Name: RING finger protein 6
Index: MW: 78092 Da pI: 9.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1156.59441156.5962-1.581900(K) (D)
1650.95441650.9179225735870(R) (L)
1694.97901694.80981001992140(R) (T)
1851.04241850.9248644254420(R) (R)
2211.21732210.951812037530(R) (L)
2444.34522444.05751188281(R) (W)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (97/685AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/53 matches (18%).
Acc. #: Species: HUMAN Name: Glycogen phosphorylase, muscle form (Myophosphorylase)
Index: MW: 97093 Da pI: 6.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1053.57581053.57332.36436500(R) (V)
1177.67301177.55301024714790(K) (F)
1262.66421262.5945557747830(K) (C)
1687.99761687.80491142Met-ox 7617731(K)DIVNMLMHHDRFK(V)
1694.97901694.8178954714831(K) (T)
1710.94671710.86784682941(K) (T)
1726.96571726.8627601Met-ox 82941(K)RIYYLSLEFYMGR(T)
1765.84421765.9489-592963100(K) (R)
1838.01021837.9700225225371(K) (V)
1994.08621994.02483145611(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (105/842AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 11/53 matches (20%).
Acc. #: Species: MOUSE Name: Glycogen phosphorylase, muscle form (Myophosphorylase)
Index: MW: 97287 Da pI: 6.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1053.57581053.56936.28338411(R) (I)
1053.57581053.57332.36436500(R) (V)
1176.59191176.5683201100(-) (R)
1177.67301177.55301024714790(K) (F)
1475.82091475.7535463603711(R) (T)
1677.91291677.8457402Met-ox 6036181(R)TIMIGGKAAPGYHMAK(M)
1687.99761687.80491142Met-ox 7617731(K)DIVNMLMHHDRFK(V)
1694.97901694.8178954714831(K) (T)
1710.94671710.86784682941(K) (T)
1726.96571726.8627601Met-ox 82941(K)RIYYLSLEFYMGR(T)
1765.84421765.9489-592963100(K) (R)
1765.84421765.9713-725565701(K) (I)
1994.08621994.02483145611(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (141/842AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 8/53 matches (15%).
Acc. #: Species: HUMAN Name: Tumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
Index: MW: 89615 Da pI: 8.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1179.64111179.5581706776850(K) (F)
1475.82091475.6841931Met-ox 2882990(K)VHFLTDPENEMK(E)
1699.86551699.9343-4067811(K) (K)
1716.93501716.8267632883011(K) (L)
1791.90801791.8601276776901(K) (R)
1852.02281851.9751264214390(K) (G)
1864.03131863.9461461160(-) (K)
1994.08621994.05001825410(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (95/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 5/53 matches (9%).
Acc. #: Species: HUMAN Name: Hypothetical protein PP2447
Index: MW: 42321 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1676.92821676.8902231401540(R) (V)
1692.96921692.8851501Met-ox 1401540(R)QNGLMSGLMQMLLLK(V)
1707.87641707.848416pyroGlu 3Met-ox 1401540(R)QNGLMSGLMQMLLLK(V)
1852.02281851.9256523363510(K) (R)
2461.27682461.3237-193533741(R) (H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (53/376AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 7/53 matches (13%).
Acc. #: Species: HUMAN Name: DNA polymerase gamma subunit 2, mitochondrial precursor (Mitochondrial DNA polymerase accessory subunit) (PolG-beta) (MtPolB) (DNA polymerase gamma accessory 55 kDa subunit) (p55)
Index: MW: 54912 Da pI: 8.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
954.4396954.5307-955121(R) (V)
1327.73941327.68584025360(R) (S)
1677.91291677.8753221091220(K) (E)
1726.96571726.89763925401(R) (G)
1805.98341805.97037.31081221(R) (E)
2176.00372176.1265-562042220(R) (Q)
2384.13542384.1597-103453641(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (78/485AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 12/53 matches (22%).
Acc. #: Species: MOUSE Name: Lamin A
Index: MW: 74210 Da pI: 6.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1176.59191176.6047-111Met-ox 1811891(K)KQLQDEMLR(R)
1350.74901350.6225945165270(K) (T)
1475.82091475.7681361Met-ox 3673781(K)LALDMEIHAYRK(L)
1676.92821676.8608402362491(R) (A)
1677.91291677.8052645855991(R) (A)
1687.99761687.8727749251(R) (I)
1699.86551699.9707-622973111(R) (Q)
1707.87641707.8050424214351(K) (T)
1729.94071729.91971212281(R) (L)
1794.87901794.8485171Met-ox 4724861(R)QNGDDPLMTYRFPPK(F)
2230.21342230.0232856466651(R) (-)
2384.13542384.1846-212412601(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (158/665AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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