MS-Fit Search Results

Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 2
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D109
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 2131 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/88(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	1.095e+007	14 (15)	25.0	15.9	-2.35	41.8	66018/8.2	HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
2	7.323e+005	13 (14)	55.0	14.8	-7.83	42.5	34274/5.7	HUMAN	60S acidic ribosomal protein P0 (L10E)
3	6.889e+004	10 (11)	26.0	11.4	2.39	52.2	61988/5.1	HUMAN	Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
4	1.884e+004	13 (14)	26.0	14.8	6.47	65.5	89504/8.0	MOUSE	Semaphorin 3E precursor (Semaphorin H) (Sema H)
5	1.799e+004	11 (12)	40.0	12.5	-12.2	36.3	34217/5.9	MOUSE	60S acidic ribosomal protein P0 (L10E)

45313	.	.	.	.	.	.	.	.	.	x	x	x	x	.	.	.	x	.	x	.	.	.	.	.	x	.	.	x	.	.	.	x	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.
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Detailed Results

1. 14/88 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1141.4848	1141.5199	-31		464	472	0	(R) (L)
1157.5454	1157.5149	26	1Met-ox	464	472	0	(R)DYQELMNTK(L)
1179.5677	1179.6010	-28		377	386	0	(K) (H)
1217.6403	1217.6166	19		634	644	0	(R) (-)
1277.6863	1277.6337	41		247	257	1	(K) (N)
1277.6863	1277.7105	-19		473	483	0	(K) (T)
1300.5143	1300.5302	-12		258	267	0	(K) (N)
1383.6749	1383.6909	-12		186	197	0	(K) (V)
1475.7425	1475.7494	-4.7		212	223	0	(K) (T)
1475.7425	1475.7858	-29		200	211	0	(R) (W)
1638.8575	1638.8604	-1.8		186	199	1	(K) (F)
1699.8208	1699.8251	-2.6	pyroGlu	418	432	0	(K)QISNLQQSISDAEQR(G)
1716.8351	1716.8517	-9.6		418	432	0	(K) (G)
1993.9874	1993.9772	5.1		224	239	0	(R) (R)
2150.0741	2150.0783	-1.9		224	240	1	(R) (R)
2384.0047	2383.9524	22		519	549	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (163/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 13/88 matches (14%).
Acc. #: Species: HUMAN Name: 60S acidic ribosomal protein P0 (L10E)
Index: MW: 34274 Da pI: 5.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
905.4470 905.4481 -1.2 4 10 1 (R) (S)
1217.6403 1217.6782 -31 17 26 0 (K) (C)
1221.6021 1221.6228 -17 67 77 0 (R) (L)
1280.6208 1280.6309 -7.9 27 38 0 (K) (Q)
1313.6733 1313.7105 -28 135 146 0 (K) (I)
1428.8021 1428.8314 -21 150 162 0 (R) (T)
1475.7425 1475.7939 -35 39 50 2 (K) (A)
1676.8088 1676.8529 -26 1Met-ox 93 106 1 (K)EDLTEIRDMLLANK(V)
1824.9546 1824.9496 2.7 84 99 1 (R) (D)
1895.9936 1896.0006 -3.7 248 264 0 (R) (V)
2194.1476 2194.1178 14 113 134 0 (R) (T)
2242.2247 2242.1865 17 1Met-ox 93 112 2 (K)EDLTEIRDMLLANKVPAAAR(A)
2752.5656 2752.4674 36 267 297 0 (K) (V)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
905.4470	905.4481	-1.2		4	10	1	(R) (S)
1217.6403	1217.6782	-31		17	26	0	(K) (C)
1221.6021	1221.6228	-17		67	77	0	(R) (L)
1280.6208	1280.6309	-7.9		27	38	0	(K) (Q)
1313.6733	1313.7105	-28		135	146	0	(K) (I)
1428.8021	1428.8314	-21		150	162	0	(R) (T)
1475.7425	1475.7939	-35		39	50	2	(K) (A)
1676.8088	1676.8529	-26	1Met-ox	93	106	1	(K)EDLTEIRDMLLANK(V)
1824.9546	1824.9496	2.7		84	99	1	(R) (D)
1895.9936	1896.0006	-3.7		248	264	0	(R) (V)
2194.1476	2194.1178	14		113	134	0	(R) (T)
2242.2247	2242.1865	17	1Met-ox	93	112	2	(K)EDLTEIRDMLLANKVPAAAR(A)
2752.5656	2752.4674	36		267	297	0	(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 55% (176/317AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/88 matches (11%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1060.5257 1060.5639 -36 224 232 0 (K) (M)
1157.5454 1157.5915 -40 250 260 0 (R) (Q)
1307.6555 1307.6782 -17 240 249 1 (R) (Q)
1837.9597 1837.9660 -3.4 374 389 0 (R) (K)
1851.9380 1851.9275 5.7 321 335 1 (K) (N)
2171.0650 2171.0252 18 271 288 0 (K) (K)
2299.1716 2299.1202 22 271 289 1 (K) (N)
2510.1640 2510.1323 13 449 471 0 (K) (I)
2705.2261 2705.1617 24 63 94 0 (R) (G)
3264.6372 3264.5144 38 339 367 0 (K) (E)

m/z Submitted	MH⁺ Matched	Delta ppm	Start	End	Missed Cleavages	Database Sequence
1060.5257	1060.5639	-36	224	232	0	(K) (M)
1157.5454	1157.5915	-40	250	260	0	(R) (Q)
1307.6555	1307.6782	-17	240	249	1	(R) (Q)
1837.9597	1837.9660	-3.4	374	389	0	(R) (K)
1851.9380	1851.9275	5.7	321	335	1	(K) (N)
2171.0650	2171.0252	18	271	288	0	(K) (K)
2299.1716	2299.1202	22	271	289	1	(K) (N)
2510.1640	2510.1323	13	449	471	0	(K) (I)
2705.2261	2705.1617	24	63	94	0	(R) (G)
3264.6372	3264.5144	38	339	367	0	(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (164/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 13/88 matches (14%).
Acc. #: Species: MOUSE Name: Semaphorin 3E precursor (Semaphorin H) (Sema H)
Index: MW: 89504 Da pI: 8.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1111.5350 1111.5570 -20 199 208 1 (R) (L)
1179.5677 1179.5251 36 1Met-ox 269 278 0 (R)LCVNDMGGQR(I)
1221.6021 1221.6479 -38 76 85 0 (R) (V)
1475.7425 1475.7211 15 266 278 1 (R) (I)
1614.8850 1614.8280 35 712 724 0 (K) (V)
1890.9642 1891.0475 -44 628 643 2 (K) (V)
2041.0499 2041.0428 3.5 1Met-ox 668 684 1 (K)ITLEVVEEHKVEGMFHK(D)
2095.0787 2094.9815 46 1Met-ox 694 711 0 (K)MPCPPLSGMSQGTKPWYK(E)
2216.1402 2216.0745 30 128 146 0 (R) (V)
2233.0799 2233.0209 26 565 584 0 (R) (T)
2282.2347 2282.1565 34 92 110 2 (R) (D)
2510.1640 2510.1887 -9.8 646 666 1 (R) (K)
2510.1640 2510.1887 -9.8 647 667 1 (K) (I)
2774.4656 2774.3533 40 86 108 2 (R) (G)
2774.4656 2774.6007 -49 1Met-ox 407 430 2 (R)IDPLMYQPIKPVHKKPILVKTDGK(Y)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1111.5350	1111.5570	-20		199	208	1	(R) (L)
1179.5677	1179.5251	36	1Met-ox	269	278	0	(R)LCVNDMGGQR(I)
1221.6021	1221.6479	-38		76	85	0	(R) (V)
1475.7425	1475.7211	15		266	278	1	(R) (I)
1614.8850	1614.8280	35		712	724	0	(K) (V)
1890.9642	1891.0475	-44		628	643	2	(K) (V)
2041.0499	2041.0428	3.5	1Met-ox	668	684	1	(K)ITLEVVEEHKVEGMFHK(D)
2095.0787	2094.9815	46	1Met-ox	694	711	0	(K)MPCPPLSGMSQGTKPWYK(E)
2216.1402	2216.0745	30		128	146	0	(R) (V)
2233.0799	2233.0209	26		565	584	0	(R) (T)
2282.2347	2282.1565	34		92	110	2	(R) (D)
2510.1640	2510.1887	-9.8		646	666	1	(R) (K)
2510.1640	2510.1887	-9.8		647	667	1	(K) (I)
2774.4656	2774.3533	40		86	108	2	(R) (G)
2774.4656	2774.6007	-49	1Met-ox	407	430	2	(R)IDPLMYQPIKPVHKKPILVKTDGK(Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (207/775AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 11/88 matches (12%).
Acc. #: Species: MOUSE Name: 60S acidic ribosomal protein P0 (L10E)
Index: MW: 34217 Da pI: 5.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
905.4470 905.4481 -1.2 4 10 1 (R) (S)
1217.6403 1217.6782 -31 17 26 0 (K) (C)
1221.6021 1221.6228 -17 67 77 0 (R) (L)
1280.6208 1280.6309 -7.9 27 38 0 (K) (Q)
1313.6733 1313.7105 -28 135 146 0 (K) (I)
1428.8021 1428.8314 -21 150 162 0 (R) (T)
1475.7425 1475.7939 -35 39 50 2 (K) (A)
1676.8088 1676.8529 -26 1Met-ox 93 106 1 (K)EDLTEIRDMLLANK(V)
1824.9546 1824.9496 2.7 84 99 1 (R) (D)
2194.1476 2194.1178 14 113 134 0 (R) (T)
2242.2247 2242.1865 17 1Met-ox 93 112 2 (K)EDLTEIRDMLLANKVPAAAR(A)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
905.4470	905.4481	-1.2		4	10	1	(R) (S)
1217.6403	1217.6782	-31		17	26	0	(K) (C)
1221.6021	1221.6228	-17		67	77	0	(R) (L)
1280.6208	1280.6309	-7.9		27	38	0	(K) (Q)
1313.6733	1313.7105	-28		135	146	0	(K) (I)
1428.8021	1428.8314	-21		150	162	0	(R) (T)
1475.7425	1475.7939	-35		39	50	2	(K) (A)
1676.8088	1676.8529	-26	1Met-ox	93	106	1	(K)EDLTEIRDMLLANK(V)
1824.9546	1824.9496	2.7		84	99	1	(R) (D)
2194.1476	2194.1178	14		113	134	0	(R) (T)
2242.2247	2242.1865	17	1Met-ox	93	112	2	(K)EDLTEIRDMLLANKVPAAAR(A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 40% (128/317AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):