MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D110
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 426 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/71(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.081e+00713 (18)47.018.3-0.43852.5 35103/5.7HUMANPyridoxine kinase (Pyridoxal kinase)
27.833e+0059 (12)38.012.7-5.7153.3 34274/5.7HUMAN60S acidic ribosomal protein P0 (L10E)
31.135e+0058 (11)33.011.3-8.2754.6 34217/5.9MOUSE60S acidic ribosomal protein P0 (L10E)
41.148e+0046 (8)14.08.5-3.5859.9 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
545366 (8)7.08.521.845.5 95432/8.3HUMANOxysterol binding protein-related protein 7 (OSBP-related protein 7) (ORP-7)
627057 (9)11.09.9-6.5764.2 82106/6.3MOUSEExostosin-2 (Multiple exostoses protein 2 homolog)
720475 (7)16.07.0-3.2034.0 37764/7.1HUMANComplex I intermediate-associated protein 30, mitochondrial precursor (CGI-65)
818216 (8)15.08.51.9950.8 47152/9.6MOUSECasein kinase I, epsilon isoform (CKI-epsilon)
912296 (8)8.08.5-3.0447.7 82945/8.5MOUSEHepatocyte growth factor precursor (Scatter factor) (SF) (Hepatopoeitin A)
1010125 (7)17.07.00.25930.6 36054/8.6HUMANGlyceraldehyde 3-phosphate dehydrogenase, liver
99623........x.x.....x...x.........x..x........x.xxx...x.........x...x......
65522.............xx....xx.x.x................x.......x.....x...............
17489.............xx....xx.x.x................x.............x...............
45313...........x.x.........x..x..........x...............x.................
62993...........x.............x..................x.x.............x.x........
81280...........x......x.....x...................x.xx........x..............
100838..........x......................x......xx.....................x.......
86798.............x.x..........................x..x....x............x.......
110274............x.....x.x.......................................x.xx.......
108737.............x..............x..x...x....................x..............


Detailed Results

1. 13/71 matches (18%).
Acc. #: Species: HUMAN Name: Pyridoxine kinase (Pyridoxal kinase)
Index: MW: 35103 Da pI: 5.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1082.51541082.5594-411621700(K) (V)
1151.64631151.6901-387160(R) (G)
1250.58031250.6057-2077860(K) (D)
1290.68951290.7282-302592690(K) (T)
1607.87771607.87193.61Met-ox 891020(K)SFLAMVVDIVQELK(Q)
1696.92301696.91216.42983121(K) (-)
1834.98041834.9988-10871021(R) (Q)
1838.96511838.95475.62762920(K) (M)
1851.01691850.9938121Met-ox 871021(R)DKSFLAMVVDIVQELK(Q)
1854.96871854.9496101Met-ox 2762920(K)AQAGEGVRPSPMQLELR(M)
1963.00931962.97731654700(K) (L)
2282.31672282.2396341401600(K) (K)
2506.42932506.3168451Met-ox 2252471(R)KVDAVFVGTGDLFAAMLLAWTHK(H)

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The matched peptides cover 47% (149/312AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/71 matches (12%).
Acc. #: Species: HUMAN Name: 60S acidic ribosomal protein P0 (L10E)
Index: MW: 34274 Da pI: 5.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1217.62741217.6782-4217260(K) (C)
1221.58881221.6228-2867770(R) (L)
1280.60321280.6309-2227380(K) (Q)
1290.68951290.67759.339481(K) (G)
1313.67551313.7105-271351460(K) (I)
1428.81031428.8314-151501620(R) (T)
1824.98631824.94962084991(R) (D)
1896.02861896.0006152482640(R) (V)
2194.19892194.1178371131340(R) (T)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 38% (123/317AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/71 matches (11%).
Acc. #: Species: MOUSE Name: 60S acidic ribosomal protein P0 (L10E)
Index: MW: 34217 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1217.62741217.6782-4217260(K) (C)
1221.58881221.6228-2867770(R) (L)
1280.60321280.6309-2227380(K) (Q)
1290.68951290.67759.339481(K) (G)
1313.67551313.7105-271351460(K) (I)
1428.81031428.8314-151501620(R) (T)
1824.98631824.94962084991(R) (D)
2194.19892194.1178371131340(R) (T)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 33% (106/317AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/71 matches (8%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.54761179.6010-453773860(K) (H)
1217.62741217.61668.86346440(R) (-)
1383.66081383.6909-221861970(K) (V)
1475.74501475.7494-3.02122230(K) (T)
1475.74501475.7858-282002110(R) (W)
1779.81791779.75093846650(R) (S)
1994.02921993.9772262242390(R) (R)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 14% (93/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/71 matches (8%).
Acc. #: Species: HUMAN Name: Oxysterol binding protein-related protein 7 (OSBP-related protein 7) (ORP-7)
Index: MW: 95432 Da pI: 8.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.54761179.5223216286360(R) (V)
1439.76871439.7065437938040(K) (F)
1838.96511838.9798-8.04945090(K) (L)
1854.96871854.9748-3.21Met-ox 4945090(K)VSMPVQLNEPLNTLQR(L)
2282.31672282.2178434905091(K) (L)
2299.27712299.1988342903091(R) (V)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 7% (61/842AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 7/71 matches (9%).
Acc. #: Species: MOUSE Name: Exostosin-2 (Multiple exostoses protein 2 homolog)
Index: MW: 82106 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.54761179.5978-431101(-) (G)
1274.62451274.6098126036110(K) (M)
1428.81031428.8175-5.01671791(R) (W)
1838.96511839.0492-464794941(K) (N)
1854.96871854.9213262522660(R) (E)
1870.98041870.9162341Met-ox 2522660(R)YFLLSSQMAIHPEYR(E)
2211.17922211.2324-241501681(R) (E)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 11% (80/718AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 5/71 matches (7%).
Acc. #: Species: HUMAN Name: Complex I intermediate-associated protein 30, mitochondrial precursor (CGI-65)
Index: MW: 37764 Da pI: 7.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1151.64631151.6312132792890(K) (V)
1696.92301696.9427-1217310(K) (F)
1795.94191795.92301032480(R) (A)
1824.98631825.0376-2816311(R) (F)
2326.22972326.22950.122692891(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 16% (54/327AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 6/71 matches (8%).
Acc. #: Species: MOUSE Name: Casein kinase I, epsilon isoform (CKI-epsilon)
Index: MW: 47152 Da pI: 9.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1217.62741217.6386-9.258690(K) (W)
1249.66301249.6285282Met-ox 58690(K)MMQGGVGIPSIK(W)
1834.98041835.0465-361001151(K) (I)
1851.01691851.0414-131Met-ox 1001151(K)FSLKTVLLLADQMISR(I)
1963.00931962.9714192602741(R) (N)
2326.22972326.1751233433651(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 15% (66/416AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 6/71 matches (8%).
Acc. #: Species: MOUSE Name: Hepatocyte growth factor precursor (Scatter factor) (SF) (Hepatopoeitin A)
Index: MW: 82945 Da pI: 8.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1210.59381210.6254-261281381(R) (G)
1274.62451274.6567-256346440(R) (C)
1290.68951290.6516291Met-ox 6346440(R)VAHLYIMGNEK(C)
2282.31672282.264823pyroGlu 5665860(K)QILNISQLVYGPEGSDLVLLK(L)
2299.27712299.2913-6.25665860(K) (L)
2326.22972326.2593-13pyroGlu 1Met-ox 4935131(K)QLRVVNGIPTQTTVGWMVSLK(Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 8% (64/728AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 5/71 matches (7%).
Acc. #: Species: HUMAN Name: Glyceraldehyde 3-phosphate dehydrogenase, liver
Index: MW: 36054 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1217.62741217.6022211Met-ox 3243340(R)VVDLMAHMASK(E)
1544.81271544.81071.32352480(R) (L)
1613.88241613.9015-1267800(K) (D)
1763.81771763.80298.43103230(K) (V)
2211.17922211.2171-172352541(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 17% (59/335AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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