MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D111
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 858 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/71(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.477e+00515 (21)45.021.133.621.0 36112/5.0HUMANEukaryotic translation initiation factor 2 subunit 1 (Eukaryotic translation initiation factor 2 alpha subunit) (eIF-2-alpha) (EIF-2alpha) (EIF-2A)
270318 (11)13.011.312.159.5 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
333807 (9)9.09.922.338.8 83873/5.9HUMANRibosomal protein S6 kinase alpha 6 (S6K-alpha 6) (90 kDa ribosomal protein S6 kinase 6) (p90-RSK 6) (Ribosomal S6 kinase 4) (RSK-4) (pp90RSK4)
429539 (12)10.012.7-4.7759.5 93868/7.0HUMANHYPOTHETICAL PROTEIN KIAA0184
529396 (8)7.08.516.665.3 98675/4.5HUMANEpidermal growth factor receptor substrate 15 (Protein Eps15) (AF-1p protein)
71845...................x............x......x.xxx..xx...x.x.......xx..x.x.x.
46726....x...x....x.....................x.x..........xx.....x...............
2448...x............x.x.......x..............x........x..x.................
112504..............x...x....x...........x...xx.....x....x......x............
107116......x...x..................x.....x..........................x.....x..


Detailed Results

1. 15/71 matches (21%).
Acc. #: Species: HUMAN Name: Eukaryotic translation initiation factor 2 subunit 1 (Eukaryotic translation initiation factor 2 alpha subunit) (eIF-2-alpha) (EIF-2alpha) (EIF-2A)
Index: MW: 36112 Da pI: 5.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1066.63771066.6261111051131(K) (H)
1164.63001164.5723501Met-ox 2672760(R)GVFNVQMEPK(V)
1230.64871230.6040361Met-ox 2352440(R)YVMTTTTLER(T)
1244.66081244.6064441441540(K) (H)
1264.65361264.6174291241330(K) (T)
1272.67241272.6265361341431(R) (R)
1320.71061320.6734281Met-ox 2662761(K)RGVFNVQMEPK(V)
1328.80291328.74734265751(R) (V)
1535.83261535.7647441421541(K) (H)
1548.83131548.7732381Met-ox 13250(K)FPEVEDVVMVNVR(S)
2125.07852125.0049351551730(K) (E)
2156.12522156.0698261912091(K) (E)
2252.19912252.1174361Met-ox 8251(R)FYQHKFPEVEDVVMVNVR(S)
2356.18592356.1495151932131(R) (A)
2434.31102434.2254351141331(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 45% (142/315AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/71 matches (11%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
973.4870973.5318-463964030(K) (V)
998.4621998.4842-22181(-) (S)
1033.55131033.5166344844920(R) (M)
1179.62851179.6010233773860(K) (H)
1217.61331217.6166-2.76346440(R) (-)
1383.73291383.6909301861970(K) (V)
1475.81061475.7494412122230(K) (T)
1475.81061475.7858172002110(R) (W)
1638.91661638.8604341861991(K) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (84/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/71 matches (9%).
Acc. #: Species: HUMAN Name: Ribosomal protein S6 kinase alpha 6 (S6K-alpha 6) (90 kDa ribosomal protein S6 kinase 6) (p90-RSK 6) (Ribosomal S6 kinase 4) (RSK-4) (pp90RSK4)
Index: MW: 83873 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
971.4759971.4661105635700(R) (Q)
1054.52411054.51696.86876950(R) (R)
1060.58121060.5526273163250(R) (R)
1131.62241131.60501568770(K) (V)
1244.66081244.66010.594925010(R) (G)
1487.82591487.7528494624731(K) (Y)
1548.83131548.7586473874000(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (73/745AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/71 matches (12%).
Acc. #: Species: HUMAN Name: HYPOTHETICAL PROTEIN KIAA0184
Index: MW: 93868 Da pI: 7.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1045.59711045.5828143213301(R) (L)
1060.58121060.5825-1.31001090(K) (R)
1094.51121094.5556-4125330(K) (T)
1179.62851179.5832384734810(K) (Q)
1230.64871230.6993-415495601(K) (G)
1232.64751232.6785-251Met-ox 1001101(K)LDGLMVTGVRR(H)
1320.71061320.6946123883990(K) (L)
1535.83261535.8738-272752861(R) (A)
1788.03071787.9808282873020(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (91/863AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/71 matches (8%).
Acc. #: Species: HUMAN Name: Epidermal growth factor receptor substrate 15 (Protein Eps15) (AF-1p protein)
Index: MW: 98675 Da pI: 4.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
992.5615992.520541pyroGlu 71780(K)QEFFVALR(L)
1009.52711009.5471-2071780(K) (L)
1144.60311144.6366-292162251(R) (A)
1179.62851179.5897337647740(K) (I)
2156.12522156.0247474314490(K) (A)
2403.24462403.1779285625840(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 7% (71/896AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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