MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D113
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1318 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/92(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
16.551e+00511 (11)19.012.022.151.9 69501/8.2HUMANBifunctional 3'-phosphoadenosine 5'-phosphosulfate synthethase 2 (PAPS synthethase 2) (PAPSS 2) (Sulfurylase kinase 2) (SK2) (SK 2) [Includes: Sulfate adenylyltransferase (Sulfate adenylate transferase) (SAT) (ATP-sulfurylase); Adenylylsulfate kinase (>
21.185e+00510 (10)17.010.917.552.5 82861/9.3HUMANPlakophilin 1 (Band-6-protein) (B6P)
34.515e+00412 (13)18.013.0-1.1755.9 81211/6.0MOUSEDNA replication licensing factor MCM7 (CDC47 homolog)
490838 (8)23.08.70.22049.6 36884/5.7MOUSEProtein c20orf77 homolog
590799 (9)28.09.8-3.2751.0 36900/5.7HUMANProtein c20orf77
6614013 (14)21.014.1-2.3643.4 82268/9.0HUMANZinc finger protein 224
760937 (7)10.07.6-23.126.5 73102/7.2HUMANDual specificity protein phosphatase 16 (Mitogen-activated protein kinase phosphatase 7) (MAP kinase phosphatase 7) (MKP-7)
849987 (7)8.07.6-20.261.1 93837/9.1HUMANGeneral control of amino acid synthesis protein 5-like 2 (Histone acetyltransferase GCN5) (hsGCN5)
940616 (6)7.06.5-1.9850.7 89504/8.0MOUSESemaphorin 3E precursor (Semaphorin H) (Sema H)
1038977 (7)14.07.616.751.5 65866/8.1HUMANKeratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
83345.....x........x...x........x.....x...................x..xx.....x..............x....x........
56028.................................x...........x......x........x....xx..x......x.x..........x.
72456............x.........x...........x........x.x...x..x...x.....x..x.....x.................x..
95271.x.....x...................................x...........x.x.........x.x.......x..............
24510.......x.....x.............................x...........x.x.........x.x.......xx.............
68039......x...............................x.x...x.x......x...........x.x..x.x...x....x...x......
77056.........x........................x.................x....................x..x.x......x......
35800.....................xxx............x....................................x....xx............
27059.....................x..x...........x.............................xx....................x...
114359......x..............x..............xx.......................x...........x..x...............


Detailed Results

1. 11/92 matches (11%).
Acc. #: Species: HUMAN Name: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthethase 2 (PAPS synthethase 2) (PAPSS 2) (Sulfurylase kinase 2) (SK2) (SK 2) [Includes: Sulfate adenylyltransferase (Sulfate adenylate transferase) (SAT) (ATP-sulfurylase); Adenylylsulfate kinase (>
Index: MW: 69501 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1066.44811066.4594-113303370(R) (K)
1085.51741085.4726415615690(K) (H)
1101.48261101.4675141Met-ox 5615690(K)AMDFYDPAR(H)
1130.56181130.5118444504580(K) (M)
1179.58851179.5468355135230(R) (K)
1340.66671340.6850-142232330(K) (L)
1383.71281383.6513443423521(R) (H)
1386.75691386.7269226036131(K) (N)
1539.84791539.77804583961(R) (E)
1940.96951940.8878421741900(K) (V)
2083.07942083.1228-212522690(K) (G)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 19% (119/614AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/92 matches (10%).
Acc. #: Species: HUMAN Name: Plakophilin 1 (Band-6-protein) (B6P)
Index: MW: 82861 Da pI: 9.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.58851179.6084-177067150(R) (E)
1265.58371265.5472291551640(R) (G)
1317.66391317.6949-237297410(R) (N)
1475.79551475.735041pyroGlu 1Met-ox 39501(R)QRVQEQVMMTVK(R)
1678.88151678.8872-3.45805951(K) (G)
1694.89351694.88216.71Met-ox 5805951(K)GLMSSGMSQLIGLKEK(G)
1707.84681707.7648485035171(R) (S)
1866.99411866.9060473964110(R) (K)
1974.02511973.9577346786930(K) (S)
2444.23342444.2033122102301(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (129/747AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 12/92 matches (13%).
Acc. #: Species: MOUSE Name: DNA replication licensing factor MCM7 (CDC47 homolog)
Index: MW: 81211 Da pI: 6.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1082.47281082.5087-332Met-ox 1071141(R)LMMEQRSR(D)
1109.50411109.4872151Met-ox 5625690(R)YIAMCHER(Q)
1181.61441181.6240-8.11101(-) (E)
1258.70441258.643249971060(K) (L)
1265.58371265.5883-3.61Met-ox 5615691(R)RYIAMCHER(Q)
1294.65281294.7020-3833430(K) (E)
1317.66391317.7014-282522630(R) (I)
1383.71281383.661937pyroGlu 5465570(R)QPPAQFEPLDMK(L)
1487.83521487.83341.26546660(R) (E)
1638.86921638.9179-305005140(R) (F)
1719.84901719.8237156746871(R) (G)
2254.13032254.1066115705890(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (134/719AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/92 matches (8%).
Acc. #: Species: MOUSE Name: Protein c20orf77 homolog
Index: MW: 36884 Da pI: 5.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1053.56071053.55802.51341431(K) (K)
1071.61181071.5951161071140(R) (S)
1258.70441258.68091936460(K) (E)
1368.74511368.7163211151260(R) (L)
1386.75691386.7759-1435461(R) (E)
1694.89351694.9706-451021141(K) (S)
1705.89581705.85502480940(R) (E)
1866.99411867.0329-2156711(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (78/326AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 9/92 matches (9%).
Acc. #: Species: HUMAN Name: Protein c20orf77
Index: MW: 36900 Da pI: 5.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1071.61181071.5951161071140(R) (S)
1083.53441083.5686-321341431(K) (K)
1258.70441258.68091936460(K) (E)
1368.74511368.7163211151260(R) (L)
1386.75691386.7759-1435461(R) (E)
1694.89351694.9706-451021141(K) (S)
1705.89581705.85502480940(R) (E)
1866.99411867.0329-2156711(R) (R)
1940.96951940.96392.91Met-ox 2572721(R)MLVEYTQNQKDVLSEK(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 28% (94/326AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 13/92 matches (14%).
Acc. #: Species: HUMAN Name: Zinc finger protein 224
Index: MW: 82268 Da pI: 9.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1068.55411068.5663-113273351(R) (M)
1199.59341199.5553324554631(K) (S)
1199.59341199.6285-294404481(R) (V)
1234.60191234.6479-371932021(R) (C)
1261.58941261.6138-19AcetN 1Met-ox 1111(-)TTFKEAMTFK(D)
1277.68171277.6862-3.665741(K) (E)
1340.66671340.66332.66216311(R) (N)
1638.86921638.8213295765881(K) (V)
1694.89351694.8614194414541(R) (L)
1707.84681707.84232.66656781(R) (T)
1723.84091723.83722.21Met-ox 6656781(R)LNLDMHQRVHMGEK(T)
1838.91191838.9448-181Met-ox 3033171(R)LNRHSMVHTAEKPFR(C)
2031.00182030.9606201271440(K) (Q)
2209.09842209.1465-221071261(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (155/707AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 7/92 matches (7%).
Acc. #: Species: HUMAN Name: Dual specificity protein phosphatase 16 (Mitogen-activated protein kinase phosphatase 7) (MAP kinase phosphatase 7) (MKP-7)
Index: MW: 73102 Da pI: 7.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1074.51521074.5544-363003101(K) (L)
1181.61441181.6604-392512610(R) (R)
1317.66391317.6763-9.34714820(K) (R)
1741.83161741.8842-302352500(K) (S)
1838.91191838.9190-3.81141300(K) (C)
1940.96951941.0162-242332501(K) (S)
2209.09842209.1406-191111301(K) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (72/665AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 7/92 matches (7%).
Acc. #: Species: HUMAN Name: General control of amino acid synthesis protein 5-like 2 (Histone acetyltransferase GCN5) (hsGCN5)
Index: MW: 93837 Da pI: 9.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1107.54091107.5879-421962030(K) (L)
1109.50411109.5380-313113190(R) (S)
1111.48371111.5271-392802890(R) (V)
1193.65751193.6054447297380(K) (N)
1741.83161741.8907-341341480(R) (S)
1940.96951940.9805-5.75896041(K) (H)
1974.02511974.0912-337297451(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (75/837AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 6/92 matches (6%).
Acc. #: Species: MOUSE Name: Semaphorin 3E precursor (Semaphorin H) (Sema H)
Index: MW: 89504 Da pI: 8.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1107.54091107.5542-121021101(K) (D)
1112.51051112.5127-2.02442510(K) (A)
1193.65751193.620631921010(R) (V)
1678.88151678.9355-324074200(R) (K)
1694.89351694.9304-221Met-ox 4074200(R)IDPLMYQPIKPVHK(K)
2233.07722233.0209255655840(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 7% (61/775AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 7/92 matches (7%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
Index: MW: 65866 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1068.55411068.5036472943020(K) (V)
1107.54091107.5435-2.33603680(K) (S)
1193.65751193.6166343813900(K) (H)
1193.65751193.6279252442531(K) (T)
1196.57091196.5985-232933021(K) (V)
1475.79551475.78586.62042150(R) (W)
1741.83161741.7490475565780(R) (Y)
1838.91191838.9149-1.771920(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 14% (96/645AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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