MS-Fit Search Results



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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D115
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 2402 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score
#/117(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.170e+00814 (11)30.012.0-1.9146.1 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
22.886e+00612 (10)19.010.3-5.5443.1 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
32.510e+00513 (11)20.011.19.0342.9 59519/5.1HUMANKeratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
48.359e+00415 (12)15.012.85.8156.7 95315/6.4MOUSEElongation factor 2 (EF-2)
57.433e+00415 (12)17.012.85.5452.2 95339/6.4HUMANElongation factor 2 (EF-2)
66.295e+0049 (7)49.07.7-4.0556.8 23400/5.7HUMANRas-related protein R-Ras2 (Ras-like protein TC21) (Teratocarcinoma oncogene)
75.551e+00410 (8)18.08.5-0.75637.0 66684/9.0HUMANZinc finger protein ZFP-36
84.649e+00411 (9)11.09.4-0.29865.3 91985/5.5MOUSEFibroblast growth factor receptor 2 precursor (FGFR-2) (Keratinocyte growth factor receptor)
94.368e+0049 (7)18.07.7-8.8251.6 55041/5.7MOUSEER81 protein (ETS translocation variant 1)
104.035e+00413 (11)20.011.1-8.7545.4 97093/6.6HUMANGlycogen phosphorylase, muscle form (Myophosphorylase)
46726x............x..........................x.......x.........x.xx.........x...x...x......x....x............x.........x..
100557.........x..xx........x......x....x.x.....x...................x...................................xx...............x.
111704.........x...........x....x............x.x...x....x.........x............x...x...........x......x...x................
39136...x....x...........x...................................x.....x.........xx.x..xx.........x..x...x..........x.x.......
57575...x....x...........x..........................x........x.....x.........x..x..xx.........x..x...x..........x.x.......
107776...x............................................x........x..........x.....x.......xx......x..x.......................
22875..x......x.................................x.....x..................x..........xx.....x........x...........x.........
41719.............x..x.x.......x.........x......x.......xx....x........x.x................................................
78861..x.....x....................x...................x.....................................x..xx...........x...x.........
56177.......x..........x..x....................x.......x.......................x...x.x...x..x.......x..x.....x............



Detailed Results

1. 14/117 matches (11%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1033.46851033.5166-464844920(R) (M)
1066.48781066.5169-272702771(K) (T)
1179.61801179.6010143773860(K) (H)
1217.57871217.6166-316346440(R) (-)
1277.71731277.71055.34734830(K) (T)
1300.56541300.5302272582670(K) (N)
1302.67941302.7018-173934031(R) (V)
1302.67941302.7157-283443550(R) (A)
1383.71091383.6909141861970(K) (V)
1475.78151475.7494222122230(K) (T)
1475.78151475.7858-2.92002110(R) (W)
1523.76381523.7892-174614721(R) (L)
1638.87791638.8604111861991(K) (F)
1716.86551716.85178.14184320(K) (G)
1993.99141993.97727.12242390(R) (R)
2384.02422383.9524305195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (194/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/117 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1060.53571060.5639-272242320(K) (M)
1065.52831065.4999271541620(K) (L)
1066.48781066.4992-112422490(K) (Q)
1095.51251095.5475-321841911(K) (G)
1121.54751121.5843-333273350(R) (N)
1140.54411140.5649-18pyroGlu 2502600(R)QGVDADINGLR(Q)
1157.60051157.59157.82502600(R) (Q)
1190.57451190.6091-292612700(R) (S)
1307.68991307.67828.92402491(R) (Q)
1837.98201837.96608.73743890(R) (K)
1851.95651851.9275163213351(K) (N)
2705.20212705.16171563940(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (120/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 13/117 matches (11%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
Index: MW: 59519 Da pI: 5.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1060.53571060.5097251Met-ox 191(-)MSVRYSSSK(H)
1090.53421090.53152.41481560(K) (L)
1109.52871109.4904353353430(K) (S)
1165.60031165.5853134424500(R) (S)
1184.57371184.5911-15pyroGlu 2462560(R)QSVEADINGLR(R)
1201.62801201.61778.62462560(R) (R)
1234.66311234.6796-132362451(R) (Q)
1300.56541300.5956-232862951(K) (N)
1434.80331434.7705234404501(K) (S)
1493.75931493.7348163233341(R) (D)
1707.80301707.77271841590(K) (G)
1796.97981797.0122-183713860(R) (Q)
1897.03761896.9489471Met-ox 1481631(K)VTMQNLNDRLASYLDK(V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (123/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 15/117 matches (12%).
Acc. #: Species: MOUSE Name: Elongation factor 2 (EF-2)
Index: MW: 95315 Da pI: 6.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1039.50701039.5320-243924000(K) (M)
1055.52771055.52690.741Met-ox 3924000(K)GPLMMYISK(M)
1086.54741086.5294172272350(K) (F)
1274.72141274.71098.34404490(K) (T)
1307.68991307.65302821320(R) (S)
1392.76461392.7309247287390(R) (L)
1434.80331434.7341482362491(K) (A)
1475.78151475.7901-5.81511621(R) (A)
1503.75561503.7670-7.62272391(K) (G)
1523.76381523.7748-7.23Met-ox 1511621(R)IKPVLMMNKMDR(A)
1707.80301707.8740-42AcetN 1Met-ox 1151(-)VNFTVDQIRAIMDK(K)
1757.97891757.97701.11Met-ox 1451591(R)QAIAERIKPVLMMNK(M)
1796.97981796.9039421Met-ox 1151(-)MVNFTVDQIRAIMDK(K)
2057.98152058.0633-406997161(R) (G)
2095.14482095.0534441Met-ox 6026191(R)LYMKARPFPDGLAEDIDK(G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (135/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 15/117 matches (12%).
Acc. #: Species: HUMAN Name: Elongation factor 2 (EF-2)
Index: MW: 95339 Da pI: 6.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1039.50701039.5320-243924000(K) (M)
1055.52771055.52690.741Met-ox 3924000(K)GPLMMYISK(M)
1086.54741086.5294172272350(K) (F)
1212.66951212.6337302402511(K) (K)
1212.66951212.6451203003091(K) (E)
1274.72141274.71098.34404490(K) (T)
1307.68991307.65302821320(R) (S)
1392.76461392.7309247287390(R) (L)
1475.78151475.7901-5.81511621(R) (A)
1503.75561503.7670-7.62272391(K) (G)
1523.76381523.7748-7.23Met-ox 1511621(R)IKPVLMMNKMDR(A)
1707.80301707.8740-42AcetN 1Met-ox 1151(-)VNFTVDQIRAIMDK(K)
1757.97891757.97701.11Met-ox 1451591(R)QAIAERIKPVLMMNK(M)
1796.97981796.9039421Met-ox 1151(-)MVNFTVDQIRAIMDK(K)
2057.98152058.0633-406997161(R) (G)
2095.14482095.0534441Met-ox 6026191(R)LYMKARPFPDGLAEDIDK(G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (147/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 9/117 matches (7%).
Acc. #: Species: HUMAN Name: Ras-related protein R-Ras2 (Ras-like protein TC21) (Teratocarcinoma oncogene)
Index: MW: 23400 Da pI: 5.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1039.50701039.4809258161(R) (L)
1217.57871217.6061-2254631(K) (L)
1276.64411276.6611-131181280(R) (A)
1351.66861351.6932-18pyroGlu 1481591(R)QLKVTYMEASAK(I)
1458.74861458.7164221621730(R) (V)
1540.78081540.8011-1385980(R) (G)
1547.75571547.7892-221161281(K) (A)
1715.84911715.7699461781910(K) (K)
1765.77081765.8431-4164790(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 49% (100/204AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 10/117 matches (8%).
Acc. #: Species: HUMAN Name: Zinc finger protein ZFP-36
Index: MW: 66684 Da pI: 9.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1036.45561036.4807-2473800(R) (W)
1060.53571060.5427-6.63033110(R) (C)
1195.58861195.5649203463540(K) (R)
1232.61281232.5952142902990(K) (H)
1351.66861351.66601.93463551(K) (H)
1523.76381523.8083-294674791(R) (C)
1525.80361525.7797161Met-ox 61721(K)SLYRNVMQETIR(N)
1638.87791638.8545142342460(K) (T)
1792.82641792.81118.63313451(R) (T)
2057.98152058.0269-221781950(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (108/582AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 11/117 matches (9%).
Acc. #: Species: MOUSE Name: Fibroblast growth factor receptor 2 precursor (FGFR-2) (Keratinocyte growth factor receptor)
Index: MW: 91985 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1066.48781066.5091-201Met-ox 5185261(K)MLKDDATEK(D)
1073.52261073.5631-383013100(K) (V)
1078.52661078.5394-1275840(K) (T)
1109.52871109.52671.71531610(R) (M)
1157.60051157.5479456506580(R) (K)
1195.58861195.6258-311661760(R) (F)
1235.62551235.5883306696780(K) (V)
1251.63781251.5832441Met-ox 6696780(K)WMAPEALFDR(V)
1276.64411276.6571-101Met-ox 6316410(R)NVLVTENNVMK(I)
1339.70781339.6680306026120(K) (G)
1351.66861351.7269-431651761(K) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (91/821AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 9/117 matches (7%).
Acc. #: Species: MOUSE Name: ER81 protein (ETS translocation variant 1)
Index: MW: 55041 Da pI: 5.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1036.45561036.4998-432062130(R) (Q)
1055.52771055.5737-443653730(K) (R)
1121.54751121.5631-143923991(R) (G)
1232.61281232.60575.721300(R) (K)
1647.78351647.8416-353603731(R) (R)
1715.84911715.810323pyroGlu 1Met-ox 2142280(R)QMSEPNIPFPPQGFK(Q)
1716.86551716.8420142142280(R) (Q)
1984.96651984.96252.01892050(R) (E)
2057.98152057.956912AcetN 1180(-)DGFYDQQVPYVVTNSQR(G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (90/477AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 13/117 matches (11%).
Acc. #: Species: HUMAN Name: Glycogen phosphorylase, muscle form (Myophosphorylase)
Index: MW: 97093 Da pI: 6.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1053.60131053.5733276436500(R) (V)
1078.52661078.5454-171Met-ox 4294380(R)MSLVEEGAVK(R)
1090.53421090.5832-457978041(R) (N)
1190.57451190.5839-7.91100(-) (R)
1234.66311234.6465131Met-ox 4294391(R)MSLVEEGAVKR(I)
1234.66311234.6465131Met-ox 4284381(R)RMSLVEEGAVK(R)
1458.74861458.7714-166106221(K) (L)
1503.75561503.7960-272442561(R) (D)
1525.80361525.8392-2331421(K) (D)
1566.81551566.7916152572700(K) (N)
1566.81551566.8168-0.807417540(R) (Q)
1647.78351647.8351-311Met-ox 6036181(R)TVMIGGKAAPGYHMAK(M)
1647.78351647.8607-474114251(R) (L)
1792.82641792.7965171711850(K) (Y)
1837.98201837.97006.55225371(K) (V)
1993.99141994.0248-1745611(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (174/842AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


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