MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D117
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1000 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/67(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	1.673e+007	13 (19)	35.0	19.4	20.9	89.4		39963/5.3		HUMAN	SH3-containing GRB2-like protein 2 (SH3 domain protein 2A) (Endophilin 1) (EEN-B1)
2	2.401e+006	12 (17)	30.0	17.9	18.2	91.0		39878/5.3		MOUSE	SH3-containing GRB2-like protein 2 (SH3 domain protein 2A) (Endophilin 1) (SH3p4)
3	9.133e+004	9 (13)	15.0	13.4	9.53	91.7		96493/5.4		MOUSE	Heat-shock protein 105 kDa (Heat shock-related 100 kDa protein E7I) (HSP-E7I) (Heat shock 110 kDa protein) (42 degrees C-HSP)
4	1.863e+004	8 (11)	17.0	11.9	41.2	71.8		72979/8.8		HUMAN	Zinc finger protein 189
5	4879	7 (10)	13.0	10.4	-10.8	72.9		55870/5.2		MOUSE	Alpha-internexin (Alpha-Inx) (66 kDa neurofilament protein) (Neurofilament-66) (NF-66)

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9357	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	x	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	x	x	.	.	x	x	.	.	.	.	.	.
102170	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	x	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	x	.	.	.
43944	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	x	x	x	.	.	.	.	.	.
59685	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.

Detailed Results

1. 13/67 matches (19%).
Acc. #: Species: HUMAN Name: SH3-containing GRB2-like protein 2 (SH3 domain protein 2A) (Endophilin 1) (EEN-B1)
Index: MW: 39963 Da pI: 5.3

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1024.4560	1024.5171	-60		68	76	0	(K) (I)
1073.5208	1073.5856	-60		253	260	1	(R) (M)
1194.5707	1194.6006	-25		185	194	1	(R) (E)
1365.8277	1365.7854	31	pyroGlu	228	239	0	(K)QAVQILQQVTVR(L)
1382.8813	1382.8120	50		228	239	0	(K) (L)
1388.7843	1388.7174	48		54	65	0	(K) (A)
1774.9631	1774.9049	33	1Met-ox	83	99	0	(K)GPGYPQAEALLAEAMLK(F)
1857.9554	1857.8547	54		297	312	0	(R) (E)
1910.0595	1910.0823	-12		228	243	1	(K) (I)
1949.0598	1948.9881	37	pyroGlu	137	152	1	(K)QNFIDPLQNLHDKDLR(E)
1966.1137	1966.0146	50		137	152	1	(K) (E)
2151.0706	2150.9487	57		103	122	0	(R) (E)
2167.0932	2166.9436	69	1Met-ox	103	122	0	(R)ELGDDCNFGPALGEVGEAMR(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (124/352AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/67 matches (17%).
Acc. #: Species: MOUSE Name: SH3-containing GRB2-like protein 2 (SH3 domain protein 2A) (Endophilin 1) (SH3p4)
Index: MW: 39878 Da pI: 5.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1024.4560 1024.5171 -60 68 76 0 (K) (I)
1073.5208 1073.5856 -60 253 260 1 (R) (M)
1194.5707 1194.6006 -25 185 194 1 (R) (E)
1365.8277 1365.7854 31 pyroGlu 228 239 0 (K)QAVQILQQVTVR(L)
1382.8813 1382.8120 50 228 239 0 (K) (L)
1388.7843 1388.7174 48 54 65 0 (K) (A)
1774.9631 1774.9049 33 1Met-ox 83 99 0 (K)GPGYPQAEALLAEAMLK(F)
1910.0595 1910.0823 -12 228 243 1 (K) (I)
1949.0598 1948.9881 37 pyroGlu 137 152 1 (K)QNFIDPLQNLHDKDLR(E)
1966.1137 1966.0146 50 137 152 1 (K) (E)
2151.0706 2150.9487 57 103 122 0 (R) (E)
2167.0932 2166.9436 69 1Met-ox 103 122 0 (R)ELGDDCNFGPALGEVGEAMR(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (108/352AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/67 matches (13%).
Acc. #: Species: MOUSE Name: Heat-shock protein 105 kDa (Heat shock-related 100 kDa protein E7I) (HSP-E7I) (Heat shock 110 kDa protein) (42 degrees C-HSP)
Index: MW: 96493 Da pI: 5.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1119.5502 1119.6162 -59 462 471 0 (R) (D)
1133.5087 1133.5591 -44 707 715 0 (R) (V)
1157.6709 1157.6465 21 160 169 0 (R) (L)
1388.7843 1388.7286 40 705 715 1 (K) (V)
1487.8118 1487.7706 28 332 346 0 (K) (I)
1707.8442 1707.9468 -60 317 331 0 (K) (A)
1857.9554 1857.8441 60 633 647 1 (R) (D)
1941.0430 1940.9718 37 357 374 1 (K) (G)
2343.1826 2343.0671 49 276 295 0 (K) (D)
2343.1826 2343.1266 24 197 217 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (135/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/67 matches (11%).
Acc. #: Species: HUMAN Name: Zinc finger protein 189
Index: MW: 72979 Da pI: 8.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1046.6277 1046.5747 51 253 261 0 (R) (C)
1251.6980 1251.6520 37 520 529 0 (K) (H)
1388.7843 1388.6844 72 87 98 1 (R) (E)
1638.9394 1638.8505 54 268 280 1 (K) (T)
1774.9631 1774.8369 71 281 294 1 (R) (K)
2082.1213 2082.0000 58 41 58 0 (K) (D)
2151.0706 2151.1535 -39 300 317 1 (R) (C)
2167.0932 2167.0381 25 435 452 0 (K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (112/626AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/67 matches (10%).
Acc. #: Species: MOUSE Name: Alpha-internexin (Alpha-Inx) (66 kDa neurofilament protein) (Neurofilament-66) (NF-66)
Index: MW: 55870 Da pI: 5.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1029.5404 1029.5580 -17 339 346 0 (R) (D)
1061.5101 1061.5227 -12 152 161 0 (R) (A)
1133.5087 1133.5703 -54 291 300 0 (K) (S)
1194.5707 1194.6231 -44 18 28 1 (K) (L)
1251.6980 1251.6407 46 387 397 0 (K) (K)
1765.8452 1765.8860 -23 471 487 0 (K) (K)
2082.1213 2082.0607 29 468 487 1 (K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (70/504AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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