MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D118
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 1840 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/97(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
13.179e+00615 (15)39.015.5-47.084.9 33688/5.0HUMANHeterogeneous nuclear ribonucleoproteins C1/C2 (hnRNP C1 / hnRNP C2)
23.098e+0058 (8)26.08.2-39.849.0 38439/5.0HUMANUNR-interacting protein (WD-40 repeat protein PT-WD) (MAP activator with WD repeats)
32.600e+00511 (11)28.011.3-57.839.5 34385/4.9MOUSEHeterogeneous nuclear ribonucleoproteins C1/C2 (hnRNP C1 / hnRNP C2)
42.221e+0059 (9)20.09.3-20.684.6 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
58.986e+0047 (7)24.07.2-40.052.9 38514/5.0MOUSEUNR-interacting protein (Serine-threonine kinase receptor-associated protein)
68.426e+0048 (8)48.08.2-53.546.8 25035/6.0HUMANAntioxidant protein 2 (1-Cys peroxiredoxin) (1-Cys PRX) (Acidic calcium-independent phospholipase A2) (aiPLA2) (Non-selenium glutathione peroxidase) (NSGPx) (24 kDa protein) (Liver 2D page spot 40) (Red blood cells page spot 12)
77.632e+00410 (10)13.010.3-12.6107 83157/8.7MOUSERibosomal protein S6 kinase alpha 2 (S6K-alpha 2) (90 kDa ribosomal protein S6 kinase 2) (p90-RSK 2) (Ribosomal S6 kinase 3) (RSK-3) (pp90RSK3)
86.218e+00412 (12)18.012.4-30.581.5 89228/7.2HUMANSemaphorin 3E precursor
95.746e+0049 (9)15.09.3-29.893.7 61937/6.0MOUSEDIHYDROPYRIMIDINASE RELATED PROTEIN-3 (DRP-3) (UNC-33-LIKE PHOSPHOPROTEIN) (ULIP PROTEIN)
102.237e+00411 (11)18.011.3-2.22108 76765/6.6HUMANProtein kinase C, alpha type (PKC-alpha) (PKC-A)
112285..x..x.x.....x.......x...xx......x...x.x....x.x........xx...........x............................
31108.................x....x..x..........................xxx...........x.....x........................
102240..x..x.x.....x.......x....x......x...x.x...............xx........................................
45313.......................x.....x........x....x....x..........x...........xx......................x.
2604......................x..x..........................xxx...........x.....x........................
19334...................x....x.................x..x......x............xx......x.......................
40308.....................x...x.xx....x.x..x...x......x..........................x....................
89894...............x.....x...x.......x....x.......x......x..x........x.......x.........x.x...........
76115.............................................x.........xx................xx..x..x.x.......x......
4226.............x.x..............x...x.....x......x.......xx......x..........x............x.........


Detailed Results

1. 15/97 matches (15%).
Acc. #: Species: HUMAN Name: Heterogeneous nuclear ribonucleoproteins C1/C2 (hnRNP C1 / hnRNP C2)
Index: MW: 33688 Da pI: 5.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
887.3270887.4085-921361420(R) (V)
903.3230903.4035-891Met-ox 1361420(R)MYSYPAR(V)
909.2550909.3074-582983060(R) (-)
943.5167943.5729-601431510(R) (A)
1123.52401123.5999-6830391(K) (Y)
1193.55161193.5836-271Met-ox 2202291(K)EQSKQAVEMK(N)
1244.55331244.6235-56AcetN 1121(-)ASNVTNKTDPR(S)
1316.72851316.7942-5018290(R) (K)
1329.61021329.6592-3751610(K) (N)
1343.60621343.6847-5831421(K) (I)
1398.66821398.7480-57pyroGlu 2052161(K)QKVDSLLENLEK(M)
1415.66801415.7746-752052161(K) (M)
1682.86461682.9151-3074890(R) (V)
1698.84501698.9100-381Met-ox 74890(R)MIAGQVLDINLAAEPK(V)
1939.86561939.6913902893061(K) (-)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 39% (120/306AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/97 matches (8%).
Acc. #: Species: HUMAN Name: UNR-interacting protein (WD-40 repeat protein PT-WD) (MAP activator with WD repeats)
Index: MW: 38439 Da pI: 5.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1022.42261022.4617-381491560(K) (Q)
1156.52341156.6326-941381481(K) (A)
1193.55161193.6002-412632720(K) (F)
1596.63061596.6706-252502620(K) (G)
1614.68301614.7552-45pyroGlu 45590(R)QGDTGDWIGTFLGHK(G)
1631.72851631.7818-3345590(R) (G)
1900.83621900.8565-112732900(R) (L)
2001.87732001.9405-321051220(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (93/350AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/97 matches (11%).
Acc. #: Species: MOUSE Name: Heterogeneous nuclear ribonucleoproteins C1/C2 (hnRNP C1 / hnRNP C2)
Index: MW: 34385 Da pI: 4.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
887.3270887.4085-921361420(R) (V)
903.3230903.4035-891Met-ox 1361420(R)MYSYPAR(V)
909.2550909.3074-583053130(R) (-)
943.5167943.5729-601431510(R) (A)
1123.52401123.5999-6830391(K) (Y)
1244.55331244.6235-56AcetN 1121(-)ASNVTNKTDPR(S)
1316.72851316.7942-5018290(R) (K)
1329.61021329.6592-3751610(K) (N)
1343.60621343.6847-5831421(K) (I)
1682.86461682.9151-3074890(R) (V)
1698.84501698.9100-381Met-ox 74890(R)MIAGQVLDINLAAEPK(V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 28% (89/313AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/97 matches (9%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.52271179.6010-663773860(K) (H)
1300.52191300.5302-6.42582670(K) (N)
1332.62151332.5200762Met-ox 2582670(K)NMQDMVEDYR(N)
1383.60521383.6909-621861970(K) (V)
1475.69071475.7494-402122230(K) (T)
1475.69071475.7858-642002110(R) (W)
1751.81341751.8272-7.96036241(R) (S)
1993.94071993.9772-182242390(R) (R)
2001.87732001.8898-6.21Met-ox 2522671(R)LDSELKNMQDMVEDYR(N)
2383.92572383.9524-115195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (131/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/97 matches (7%).
Acc. #: Species: MOUSE Name: UNR-interacting protein (Serine-threonine kinase receptor-associated protein)
Index: MW: 38514 Da pI: 5.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1156.52341156.6326-941381481(K) (A)
1193.55161193.6002-412632720(K) (F)
1596.63061596.6706-252502620(K) (G)
1614.68301614.7552-45pyroGlu 45590(R)QGDTGDWIGTFLGHK(G)
1631.72851631.7818-3345590(R) (G)
1900.83621900.8565-112732900(R) (L)
2001.87732001.9405-321051220(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (85/351AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 8/97 matches (8%).
Acc. #: Species: HUMAN Name: Antioxidant protein 2 (1-Cys peroxiredoxin) (1-Cys PRX) (Acidic calcium-independent phospholipase A2) (aiPLA2) (Non-selenium glutathione peroxidase) (NSGPx) (24 kDa protein) (Liver 2D page spot 40) (Red blood cells page spot 12)
Index: MW: 25035 Da pI: 6.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1085.53971085.5995-55981060(K) (N)
1191.57031191.6737-871451550(K) (N)
1356.74241356.8215-581631741(R) (V)
1409.63391409.6735-2842530(R) (A)
1596.63061596.6852-3485970(K) (L)
1896.89811897.0071-5768840(K) (D)
1900.83621900.9942-831Met-ox 1091251(R)ELAILLGMLDPAEKDEK(G)
2030.93612030.9877-2525410(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 48% (108/224AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 10/97 matches (10%).
Acc. #: Species: MOUSE Name: Ribosomal protein S6 kinase alpha 2 (S6K-alpha 2) (90 kDa ribosomal protein S6 kinase 2) (p90-RSK 2) (Ribosomal S6 kinase 3) (RSK-3) (pp90RSK3)
Index: MW: 83157 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1123.52401123.5682-396526600(K) (L)
1193.55161193.5955-371241330(K) (L)
1283.60061283.7112-864634730(R) (D)
1285.61041285.6363-206937040(K) (T)
1316.72851316.6747414814900(K) (G)
1327.62381327.62042.54194300(K) (R)
1332.62151332.6696-361Met-ox 4814900(K)YVYLVMELMR(G)
1356.74241356.6469701Met-ox 79910(K)VTGSDAGQLYAMK(V)
1483.83461483.7215764194311(K) (C)
2056.95452057.0564-504814971(K) (I)
2056.95452057.0780-606766921(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (102/733AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 12/97 matches (12%).
Acc. #: Species: HUMAN Name: Semaphorin 3E precursor
Index: MW: 89228 Da pI: 7.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
964.4640964.4376273964030(K) (F)
1123.52401123.5682-391992081(R) (L)
1193.55161193.6067-465475561(R) (R)
1316.72851316.7313-2.26676771(R) (V)
1332.62151332.6734-393743850(K) (V)
1415.66801415.7456-554874990(K) (R)
1614.68301614.66819.25345460(R) (Y)
1614.68301614.8280-907127240(K) (V)
1698.84501698.9325-522692831(R) (W)
1896.89811896.88029.42242391(K) (D)
2030.93612030.8766291Met-ox 2272431(K)FVGSYMIPDNEDRDDNK(V)
2116.88852117.0234-64921081(K) (G)
2132.83742133.0183-851Met-ox 921081(K)EIHWPSTALKMEECIMK(G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (142/775AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 9/97 matches (9%).
Acc. #: Species: MOUSE Name: DIHYDROPYRIMIDINASE RELATED PROTEIN-3 (DRP-3) (UNC-33-LIKE PHOSPHOPROTEIN) (ULIP PROTEIN)
Index: MW: 61937 Da pI: 6.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1409.63391409.6598-181601710(K) (D)
1682.86461682.7922431Met-ox 1581711(K)EKGVNSFMVYMAYK(D)
1698.84501698.7871342Met-ox 1581711(K)EKGVNSFMVYMAYK(D)
2030.93612030.9896-265325500(R) (S)
2052.82022052.9449-611Met-ox 4244400(K)NHQSVAEYNIFEGMELR(G)
2069.85382070.0305-8564810(K) (G)
2085.95592086.0254-331Met-ox 64810(K)MVIPGGIDVHTHFQMPYK(G)
2101.82512102.0203-932Met-ox 64810(K)MVIPGGIDVHTHFQMPYK(G)
2225.10372225.1674-292392581(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (88/570AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 11/97 matches (11%).
Acc. #: Species: HUMAN Name: Protein kinase C, alpha type (PKC-alpha) (PKC-A)
Index: MW: 76765 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
943.5167943.4597603263330(K) (V)
964.4640964.5216-60pyroGlu 28351(R)QKNVHEVK(D)
1307.60181307.5183641321410(K) (Q)
1323.53041323.5132131Met-ox 1321410(K)CDTCDMNVHK(Q)
1350.60561350.7125-795645751(K) (H)
1474.66591474.7443-532392491(R) (T)
1682.86461682.73078078910(R) (G)
1698.84501698.8128192172300(R) (L)
1838.86081838.83181677911(K) (G)
1838.86081838.85045.763760(K) (R)
2052.82022052.9469-621Met-ox 3723881(K)KDVVIQDDDVECTMVEK(R)
2144.85892144.9237-302Met-ox 4794961(K)IADFGMCKEHMMDGVTTR(T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (127/672AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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