MS-Fit Search Results


.

Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D119
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 2642 entries (results displayed for top 15 matches).

Results Summary

MOWSE
Score		#/121(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.106e+01324 (19)44.019.8-1.6142.8 59519/5.1HUMANKeratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
21.225e+00814 (11)31.011.6-13.474.9 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
32.613e+00512 (9)17.09.92.5294.5 89615/8.6HUMANTumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
42.108e+0059 (7)20.07.4-10.679.6 60870/6.0MOUSEEH-domain containing protein 3
51.621e+00512 (9)19.09.94.5453.6 69501/8.2HUMANBifunctional 3'-phosphoadenosine 5'-phosphosulfate synthethase 2 (PAPS synthethase 2) (PAPSS 2) (Sulfurylase kinase 2) (SK2) (SK 2) [Includes: Sulfate adenylyltransferase (Sulfate adenylate transferase) (SAT) (ATP-sulfurylase); Adenylylsulfate kinase (>
61.446e+00512 (9)26.09.9-19.346.6 65866/8.1HUMANKeratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
77.480e+0049 (7)8.07.4-28.762.3 79542/9.4HUMANBromodomain-containing protein 3 (RING3-like protein)
86.176e+00411 (9)20.09.1-25.683.2 90673/9.4HUMANZinc finger protein ZFD25
95.958e+0049 (7)18.07.4-6.8278.6 79687/9.0HUMANEstradiol 17 beta-dehydrogenase 4 (17-beta-HSD 4) (17-beta-hydroxysteroid dehydrogenase 4)
105.391e+00410 (8)33.08.3-5.9284.1 31627/8.6MOUSESteroidogenic acute regulatory protein, mitochondrial precursor (StAR) (StARD1) (Luteinizing hormone-induced protein)
115.048e+0049 (7)24.07.40.71252.5 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
123.828e+00411 (9)23.09.124.978.9 67255/9.1MOUSEZinc finger protein 37 (Zfp-37) (Male germ cell specific zinc finger protein)
133.541e+0049 (7)26.07.41.7868.7 33688/5.0HUMANHeterogeneous nuclear ribonucleoproteins C1/C2 (hnRNP C1 / hnRNP C2)
143.523e+00411 (9)25.09.1-23.076.2 73141/6.7MOUSEPutative ATP-dependent RNA helicase PL10
152.880e+0047 (5)17.05.85.6170.4 48634/5.7HUMAN26S protease regulatory subunit 7 (MSS1 protein)
108237.............x...........xx.x..x..x.x.x..x............xx..x..x.....x.....x.....x..................x.xxxx...x........x.x..
45313........x...............x....x..x......xxxx..............x......x.....x....x............x.....................x..........
42717...................x............x.x..........x..................x..x.......x..x...x.....x...........x..........x.........
49909.....................................x.....x.....x........x..x.......x.........................xx..........x.............
80772.............x..........x.......x..........x..x..........x.x........x...........x......x....x..........x.................
110808........x...........x...x..x.....x.......x...x..x..............xx................x...................................x...
2755....................x..........x........x................x.........x...........................xx................x.x.....
43450...........................x..x..................x..x......x...xxxx.....x............x...................................
111961...................................x...x................x..............................x...........x.xxx...x.............
112207.................................................x.x.x.....x...x.............xx.................................xx.x.....
97443........................x..................x..x...............................x.x.x......................x.......x......x
92003..........................x...x.x...x...................x..........x..........x.x.xx..........................x..........
112285x.....................x..........x..........x...x.....................xxx............................x...................
98092...............................x...x.....x...x..x........................x...x...x.....x....x....x.......................
3504......................x........x..x.....................x...........x................................xx..................


Detailed Results

1. 24/121 matches (19%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
Index: MW: 59519 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1003.51491003.5536-393633701(K) (N)
1090.50751090.5315-221481560(K) (L)
1106.46811106.5264-531Met-ox 1481560(K)VTMQNLNDR(L)
1109.47491109.4904-143353430(K) (S)
1165.57781165.5853-6.54424500(R) (S)
1201.61991201.61771.92462560(R) (R)
1234.66851234.6796-9.02362451(R) (Q)
1262.59881262.59770.854514640(R) (G)
1300.54591300.5956-382862951(K) (N)
1365.62901365.6399-7.93233330(R) (K)
1373.65151373.6549-2.4pyroGlu 3873990(K)QSLEASLAETEGR(Y)
1390.69191390.68147.63873990(K) (Y)
1434.77171434.77050.814404501(K) (S)
1493.74201493.73484.83233341(R) (D)
1707.77691707.77272.541590(K) (G)
1796.99461797.0122-9.83713860(R) (Q)
2210.12532210.04783536591(R) (G)
2212.14672212.0985223443621(K) (S)
2224.18802224.1423212672851(K) (N)
2240.17002240.1372151Met-ox 2672851(K)ADLEMQIESLTEELAYLKK(N)
2281.09582281.01283610351(K) (I)
2367.27932367.26326.82082280(K) (L)
2872.42432872.3933112963220(R) (S)
3052.62163052.6279-2.02022281(K) (L)

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The matched peptides cover 44% (265/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 14/121 matches (11%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
973.4954973.5318-373964030(K) (V)
1066.44571066.5169-672702771(K) (T)
1125.45861125.5428-753673760(K) (Y)
1179.60191179.60100.773773860(K) (H)
1265.62741265.6378-8.12782880(R) (K)
1277.70951277.6337592472571(K) (N)
1277.70951277.7105-0.784734830(K) (T)
1300.54591300.5302122582670(K) (N)
1302.62551302.7018-593934031(R) (V)
1302.62551302.7157-693443550(R) (A)
1383.69701383.69094.41861970(K) (V)
1475.76741475.7494122122230(K) (T)
1475.76741475.7858-122002110(R) (W)
1599.78451599.8342-314424551(K) (E)
1716.86241716.85176.34184320(K) (G)
1993.99851993.9772112242390(R) (R)
2384.01352383.9524265195490(R) (G)

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The matched peptides cover 31% (206/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 12/121 matches (9%).
Acc. #: Species: HUMAN Name: Tumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
Index: MW: 89615 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1036.45161036.4951-427507590(R) (Q)
1179.60191179.5581376776850(K) (F)
1201.61991201.5424647647740(R) (C)
1320.59031320.6548-497507611(R) (C)
1475.76741475.6841561Met-ox 2882990(K)VHFLTDPENEMK(E)
1493.74201493.6921336226320(K) (E)
1716.86241716.8267212883011(K) (L)
1791.77301791.8601-496776901(K) (R)
1851.93701851.9751-214214390(K) (G)
1993.99851994.0500-2625410(R) (T)
2212.14672212.01426046611(R) (E)
2495.06202495.2023-562Met-ox 3653861(R)REGHAQNPMEPSVPQLSLMDVK(C)

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The matched peptides cover 17% (136/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/121 matches (7%).
Acc. #: Species: MOUSE Name: EH-domain containing protein 3
Index: MW: 60870 Da pI: 6.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1236.56731236.6489-661251350(K) (F)
1307.67781307.613349AcetN 1111(-)FSWLGNDDRR(K)
1332.67501332.6582132212310(K) (V)
1390.69191390.7331-303303410(K) (I)
1434.77171434.71164214260(K) (K)
1566.75411566.7586-2.93583701(K) (F)
2117.90952118.0105-484444610(R) (I)
2133.91802134.0054-411Met-ox 4444610(R)DKPMYDEIFYTLSPVDGK(I)
2367.27932367.3076-125105301(K) (R)

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The matched peptides cover 20% (110/535AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 12/121 matches (9%).
Acc. #: Species: HUMAN Name: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthethase 2 (PAPS synthethase 2) (PAPSS 2) (Sulfurylase kinase 2) (SK2) (SK 2) [Includes: Sulfate adenylyltransferase (Sulfate adenylate transferase) (SAT) (ATP-sulfurylase); Adenylylsulfate kinase (>
Index: MW: 69501 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1003.51491003.5172-2.47141(K) (S)
1066.44571066.4594-133303370(R) (K)
1179.60191179.5468475135230(R) (K)
1307.67781307.6418285135241(R) (D)
1323.63411323.6367-1.91Met-ox 5135241(R)DPAGMPHPETKK(D)
1383.69701383.6513333423521(R) (H)
1405.63691405.6422-3.71Met-ox 4504601(K)DDDVPLDWRMK(Q)
1497.73891497.7562-1215270(K) (N)
1837.95481837.93839.038551(R) (T)
1940.95571940.8878351741900(K) (V)
2083.02492083.1228-472522690(K) (G)
2281.09582281.1352-171741931(K) (T)

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The matched peptides cover 19% (119/614AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 12/121 matches (9%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
Index: MW: 65866 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
973.4954973.5318-374004070(K) (V)
1037.51161037.5267-152452530(R) (T)
1066.44571066.5169-672742811(K) (T)
1107.50631107.5435-343603680(K) (S)
1193.60051193.6166-143813900(K) (H)
1193.60051193.6279-232442531(K) (T)
1300.54591300.5812-271Met-ox 1101(-)MSCQISCKSR(G)
1320.59031320.58345.346610(R) (S)
1329.65881329.6399144254360(K) (G)
1329.65881329.7266-513483590(R) (A)
1460.76621460.8001-233093200(K) (V)
1475.76741475.7858-122042150(R) (W)
1838.92311838.91494.471920(K) (G)
3048.52123048.49488.63213470(K) (N)

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The matched peptides cover 26% (168/645AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 9/121 matches (7%).
Acc. #: Species: HUMAN Name: Bromodomain-containing protein 3 (RING3-like protein)
Index: MW: 79542 Da pI: 9.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1037.51161037.50902.54044110(K) (F)
1165.57781165.6040-224034111(R) (F)
1277.70951277.7806-565926021(K) (E)
1383.69701383.7095-9.04044141(K) (M)
1493.74201493.7447-1.82462600(K) (S)
2117.90952118.0615-724154350(K) (G)
2133.91802134.0564-651Met-ox 4154350(K)MPDEPVEAPALPAPAAPMVSK(G)
2705.20692705.3279-454154411(K) (S)
2737.34482737.31779.92Met-ox 4154411(K)MPDEPVEAPALPAPAAPMVSKGAESSR(S)

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The matched peptides cover 8% (65/726AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 11/121 matches (9%).
Acc. #: Species: HUMAN Name: Zinc finger protein ZFD25
Index: MW: 90673 Da pI: 9.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1107.50631107.5798-662802890(R) (Q)
1156.56281156.5131431901981(K) (Q)
1332.67501332.6912-127567670(R) (K)
1344.63951344.7098-522652751(R) (E)
1405.63691405.7228-615885990(K) (I)
1405.63691405.7228-612522630(K) (R)
1460.76621460.7862-147567681(R) (I)
1475.76741475.76074.67007121(K) (I)
1487.78471487.8044-136566671(K) (E)
1487.78471487.8044-133483591(K) (E)
1489.67481489.7586-561121230(K) (R)
1489.67481489.7837-732362471(K) (E)
1698.92941698.82336259740(K) (I)
1907.85551907.9438-467407551(K) (A)

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The matched peptides cover 20% (160/783AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 9/121 matches (7%).
Acc. #: Species: HUMAN Name: Estradiol 17 beta-dehydrogenase 4 (17-beta-HSD 4) (17-beta-hydroxysteroid dehydrogenase 4)
Index: MW: 79687 Da pI: 9.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1232.58341232.6197-294254350(K) (G)
1265.62741265.6642-297037131(K) (L)
1379.65961379.7105-372592701(R) (A)
1940.95571941.0347-416466631(K) (W)
2211.15072211.2290-354044221(K) (L)
2224.18802224.1059376827020(K) (L)
2240.17002240.1008311Met-ox 6827020(K)GAADTTIILSDEDFMEVVLGK(L)
2281.09582281.1439-214524720(K) (R)
2367.27932367.1291631Met-ox 1461680(R)IIMTSSASGIYGNFGQANYSAAK(L)

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The matched peptides cover 18% (136/736AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 10/121 matches (8%).
Acc. #: Species: MOUSE Name: Steroidogenic acute regulatory protein, mitochondrial precursor (StAR) (StARD1) (Luteinizing hormone-induced protein)
Index: MW: 31627 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1332.67501332.6218402732841(K) (-)
1342.62451342.7119-6585960(K) (K)
1351.65891351.6363178181(K) (N)
1405.63691405.62448.91401510(R) (E)
1460.76621460.69564839520(R) (R)
1765.75631765.8770-681151(-) (H)
1791.77301791.8410-381Met-ox 1401541(R)MEAMGEWNPNVKEIK(V)
2565.21502565.19009.71Met-ox 1932160(R)GSTCVLAGMATHFGEMPEQSGVIR(A)
2705.20692705.2962-331922161(R) (A)
2737.34482737.2860212Met-ox 1922161(R)RGSTCVLAGMATHFGEMPEQSGVIR(A)

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The matched peptides cover 33% (96/284AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

11. 9/121 matches (7%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1066.44571066.4992-502422490(K) (Q)
1307.67781307.6782-0.322402491(R) (Q)
1323.63411323.6731-291Met-ox 2402491(R)IKFEMEQNLR(Q)
1791.77301791.7283254905120(R) (G)
1837.95481837.9660-6.13743890(R) (K)
1851.93701851.92755.23213351(K) (N)
2299.19212299.1202312712891(K) (N)
2705.20692705.16171763940(R) (G)
3223.32793223.2821145796180(R) (S)

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The matched peptides cover 24% (155/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

12. 11/121 matches (9%).
Acc. #: Species: MOUSE Name: Zinc finger protein 37 (Zfp-37) (Male germ cell specific zinc finger protein)
Index: MW: 67255 Da pI: 9.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1106.46811106.5078-361521610(K) (K)
1156.56281156.5785-14pyroGlu 1Met-ox 5745831(K)QNASLTKHMK(I)
1179.60191179.59456.34064151(K) (I)
1234.66851234.6028531521621(K) (K)
1379.65961379.6113351Met-ox 1130(-)MATSEPAESDAVR(A)
1493.74201493.6960314594710(K) (T)
1791.77301791.76424.91Met-ox 33480(K)LENCSNPASMGNQDPK(Q)
1837.95481837.8213733603741(R) (S)
1851.93701851.8006743043180(R) (A)
1870.93831870.8104685285420(R) (A)
2384.01352384.0651-222762950(R) (S)

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The matched peptides cover 23% (138/594AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

13. 9/121 matches (7%).
Acc. #: Species: HUMAN Name: Heterogeneous nuclear ribonucleoproteins C1/C2 (hnRNP C1 / hnRNP C2)
Index: MW: 33688 Da pI: 5.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
943.5603943.5729-131431510(R) (A)
1062.45151062.5254-692242321(K) (S)
1193.60051193.5836141Met-ox 2202291(K)EQSKQAVEMK(N)
1316.78551316.7942-6.618290(R) (K)
1329.65881329.6592-0.2451610(K) (N)
1599.78451599.6902591311421(R) (V)
1682.90991682.9151-3.174890(R) (V)
1698.92941698.9100111Met-ox 74890(R)MIAGQVLDINLAAEPK(V)
2224.18802224.13372443611(K) (N)

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The matched peptides cover 26% (81/306AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

14. 11/121 matches (9%).
Acc. #: Species: MOUSE Name: Putative ATP-dependent RNA helicase PL10
Index: MW: 73141 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1165.57781165.6594-7038461(R) (E)
1232.58341232.55482363731(K) (S)
1232.58341232.58121.85916011(R) (Q)
1300.54591300.6398-724784871(R) (S)
1320.59031320.6800-682762860(R) (K)
1329.65881329.5972462Met-ox 3763860(R)HTMMFSATFPK(E)
1329.65881329.6837-191Met-ox 3623721(R)RIVEQDTMPPK(G)
1707.77691707.8964-703113251(R) (L)
1765.75631765.7741-105996151(R) (A)
1838.92311838.9513-155315471(R) (N)
1940.95571940.9792-123353501(K) (M)
2083.02492083.02490.0235115270(K) (I)
2144.91442144.9864-345645800(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (170/660AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

15. 7/121 matches (5%).
Acc. #: Species: HUMAN Name: 26S protease regulatory subunit 7 (MSS1 protein)
Index: MW: 48634 Da pI: 5.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1062.45151062.4856-3235430(K) (Q)
1165.57781165.5312401100(-) (K)
1201.61991201.61127.31Met-ox 3573661(K)IHARSMSVER(D)
1379.65961379.5828562852970(R) (T)
1497.73891497.8025-4285970(K) (C)
2224.18802224.16958.33173360(K) (L)
2240.17002240.16442.51Met-ox 3173360(K)VLMATNRPDTLDPALMRPGR(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (75/433AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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