MS-Fit Search Results



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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D124
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 545 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/100(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
13.544e+00613 (13)35.013.0-21.427.1 38275/5.4HUMANProtein C14orf3 (Protein HSPC322)
218385 (5)11.05.03.6064.2 41941/6.0HUMANCholinephosphate cytidylyltransferase B (Phosphorylcholine transferase B) (CTP:phosphocholine cytidylyltransferase B) (CT B) (CCT B) (CCT-beta)
310808 (8)7.08.0-13.650.2 87998/5.2HUMANTranslocation protein SEC63 homolog
46397 (7)6.07.0-11.734.8 99871/5.3HUMANLYSp100 protein (Lymphoid-restricted homolog of Sp100) (Nuclear autoantigen Sp-140) (Speckled 140 kDa) (Nuclear body protein Sp140)
55775 (5)6.05.0-11.449.3 65866/8.1HUMANKeratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
52361................x.......x............x............x............x................x.....x.x...xx..x.xx
25434.................x.............................................x........................x.x..x......
111051....................x......................x............x..x...x.x......................x.........x.
55247................x..............x........x...................................xx..x..........x........
114359...........................................................x...x.............x..x....x..............



Detailed Results

1. 13/100 matches (13%).
Acc. #: Species: HUMAN Name: Protein C14orf3 (Protein HSPC322)
Index: MW: 38275 Da pI: 5.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
899.4292899.4375-9.33293360(K) (L)
919.4297919.4565-293223280(R) (Q)
955.5311955.5689-401952030(K) (I)
997.4849997.4954-113063140(R) (T)
1057.45751057.5067-47AcetN 1101(-)AKWGEGDPR(W)
1254.62781254.6442-133063161(R) (Q)
1484.71101484.7273-112132240(K) (V)
1713.75441713.7734-1117300(R) (D)
1799.86281799.8757-7.23223361(R) (L)
1940.00171940.0381-192092241(K) (V)
2136.93792136.9912-251Met-ox 2502680(K)FHMVDGNVSGEFTDLVPEK(H)
2445.20192445.2414-162252460(R) (G)
2687.26912687.3793-412252491(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (119/338AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/100 matches (5%).
Acc. #: Species: HUMAN Name: Cholinephosphate cytidylyltransferase B (Phosphorylcholine transferase B) (CTP:phosphocholine cytidylyltransferase B) (CT B) (CCT B) (CCT-beta)
Index: MW: 41941 Da pI: 6.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
901.3960901.4056-112122180(R) (R)
1057.45751057.5067-472122191(R) (N)
1713.75441713.7125243533690(K) (-)
1729.75741729.7074291Met-ox 3533690(K)AASASISSMSEGDEDEK(-)
1940.00171939.9594222843000(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (42/369AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/100 matches (8%).
Acc. #: Species: HUMAN Name: Translocation protein SEC63 homolog
Index: MW: 87998 Da pI: 5.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
911.3938911.4079-151Met-ox 2382441(K)TRNMDMK(R)
973.4298973.4703-4238450(R) (L)
1018.43561018.4805-446006071(R) (D)
1041.42721041.4498-221361440(K) (I)
1057.45751057.4447121Met-ox 1361440(K)GGDEVMFMR(I)
1073.46161073.4396202Met-ox 1361440(K)GGDEVMFMR(I)
1713.75441713.7365102Met-ox 1311441(K)YHPDKGGDEVMFMR(I)
2445.20192445.2724-294514730(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 7% (60/760AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/100 matches (7%).
Acc. #: Species: HUMAN Name: LYSp100 protein (Lymphoid-restricted homolog of Sp100) (Nuclear autoantigen Sp-140) (Speckled 140 kDa) (Nuclear body protein Sp140)
Index: MW: 99871 Da pI: 5.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
899.4292899.4198108388450(K) (L)
931.4101931.4485-41pyroGlu 4024100(R)QEASSSLAR(R)
964.4246964.4410-176146210(K) (W)
1159.51141159.5128-1.1pyroGlu 7547620(R)QMCPEEQLK(C)
1176.53871176.5393-0.527547620(R) (C)
1254.62781254.6370-7.48468551(R) (E)
1765.73391765.7777-253503640(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 6% (59/882AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/100 matches (5%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
Index: MW: 65866 Da pI: 8.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1041.42721041.4531-25180(-) (S)
1057.45751057.44808.91Met-ox 180(-)MSCQISCK(S)
1176.53871176.5537-133713800(K) (Y)
1254.62781254.60791621340(R) (R)
1475.72021475.7858-442042150(R) (W)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 6% (44/645AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


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