MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.07.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D125
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 114561
Molecular weight search (1000 - 100000 Da) selects 108217 entries.
Full pI range: 114561 entries.
Species search ( HUMAN MOUSE ) selects 14035 entries.
Combined molecular weight, pI and species searches select 12424 entries.
Pre searches select 12424 entries.

Data Set 1 Results

MS-Fit search selects 641 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/89(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	1.806e+004	6 (6)	17.0	6.7	16.4	71.2		58629/9.1		MOUSE	T-box transcription factor TBX6 (T-box protein 6)
2	1.414e+004	8 (8)	10.0	9.0	13.3	60.6		82806/9.1		HUMAN	Poly(A) polymerase gamma (PAP gamma) (Polynucleotide adenylyltransferase gamma) (SRP RNA 3' adenylating enzyme)
3	4554	5 (5)	15.0	5.6	25.5	30.3		66018/8.2		HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
4	2871	6 (6)	7.0	6.7	4.15	45.1		80294/5.6		MOUSE	Adseverin (Scinderin) (Gelsolin-like protein)
5	2770	5 (5)	9.0	5.6	3.59	30.7		46753/5.1		HUMAN	snRNA activating protein complex 50 kDa subunit (SNAPc 50 kDa subunit) (Proximal sequence element-binding transcription factor beta subunit) (PSE-binding factor beta subunit) (PTF beta subunit)

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17988	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	x	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.
23887	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 6/89 matches (6%).
Acc. #: Species: MOUSE Name: T-box transcription factor TBX6 (T-box protein 6)
Index: MW: 58629 Da pI: 9.1

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1255.5722	1255.6323	-48		6	16	0	(R) (L)
1837.9417	1837.8808	33		172	187	0	(R) (Q)
2211.1800	2211.0835	44		17	37	0	(R) (Y)
2283.2675	2283.2171	22		373	393	0	(R) (G)
2284.2680	2284.1616	47		394	413	0	(R) (G)
2705.3180	2705.3159	0.77	1Met-ox	172	194	1	(R)VYIHPDSPATGAHWMRQPVSFHR(V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (96/540AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/89 matches (8%).
Acc. #: Species: HUMAN Name: Poly(A) polymerase gamma (PAP gamma) (Polynucleotide adenylyltransferase gamma) (SRP RNA 3' adenylating enzyme)
Index: MW: 82806 Da pI: 9.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1044.4659 1044.5148 -47 pyroGlu 1Met-ox 469 477 0 (R)QANNINMLK(E)
1045.5828 1045.5464 35 469 477 0 (R) (E)
1271.6314 1271.6418 -8.1 110 121 0 (K) (H)
1377.7817 1377.7742 5.4 205 216 0 (R) (E)
1662.9173 1662.8411 46 507 522 1 (K) (R)
1766.9061 1766.8350 40 pyroGlu 288 302 0 (K)QSEESNLNLPVWDPR(V)
1874.9596 1874.9264 18 429 443 0 (K) (R)
1890.9543 1890.9213 17 1Met-ox 429 443 0 (K)DNNYVSMWFLGIIFR(R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (79/736AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/89 matches (5%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1179.6245 1179.6010 20 377 386 0 (K) (H)
1475.8051 1475.7494 38 212 223 0 (K) (T)
1475.8051 1475.7858 13 200 211 0 (R) (W)
1994.0499 1993.9772 36 224 239 0 (R) (R)
2300.2091 2300.1985 4.6 344 364 1 (R) (S)
2384.0513 2383.9524 41 519 549 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (102/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/89 matches (6%).
Acc. #: Species: MOUSE Name: Adseverin (Scinderin) (Gelsolin-like protein)
Index: MW: 80294 Da pI: 5.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1151.6674 1151.6173 44 501 511 1 (K) (L)
1837.9417 1837.9772 -19 119 137 0 (K) (R)
1874.9596 1874.9421 9.3 206 222 0 (R) (V)
1890.9543 1890.9370 9.1 1Met-ox 206 222 0 (R)SQLIVVEEGSEPSELMK(V)
1994.0499 1994.0783 -14 119 138 1 (K) (L)
2300.2091 2300.2172 -3.5 206 226 1 (R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 7% (52/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/89 matches (5%).
Acc. #: Species: HUMAN Name: snRNA activating protein complex 50 kDa subunit (SNAPc 50 kDa subunit) (Proximal sequence element-binding transcription factor beta subunit) (PSE-binding factor beta subunit) (PTF beta subunit)
Index: MW: 46753 Da pI: 5.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1293.6086 1293.6010 5.9 328 337 0 (R) (T)
1389.7301 1389.7068 17 41 52 0 (R) (G)
1758.9426 1758.9213 12 197 211 0 (K) (L)
1774.9264 1774.9162 5.8 1Met-ox 197 211 0 (K)EHKPYQTMLVLGSQK(L)
2024.0295 2024.0751 -23 195 211 1 (K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (39/411AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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