MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D128
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 2190 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/113(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
13.135e+00816 (14)32.014.2-4.3541.4 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
22.356e+00612 (10)22.010.6-2.2023.6 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
31.019e+0058 (7)12.07.117.256.1 99762/4.6MOUSENeural-cadherin precursor (N-cadherin) (Cadherin-2)
45.924e+0047 (6)11.06.22.4948.8 96866/5.3HUMANHeat-shock protein 105 kDa (Heat shock 110 kDa protein) (Antigen NY-CO-25)
55.834e+0047 (6)18.06.26.3657.6 68469/4.6HUMANInvolucrin
64.756e+0048 (7)14.07.1-3.3852.4 87799/6.7HUMANGlutaminyl-tRNA synthetase (Glutamine--tRNA ligase) (GlnRS)
74.110e+0049 (7)13.08.09.4737.1 82468/5.9HUMANADAM 2 precursor (A disintegrin and metalloproteinase domain 2) (Fertilin beta subunit) (PH-30) (PH30)
83.520e+00410 (8)14.08.8-1.5049.3 93305/5.8HUMANHigh-affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
93.505e+00410 (8)17.08.8-5.7257.9 75793/6.4HUMANSerine/threonine protein phosphatase with EF-hands-1 (PPEF-1) (Protein phosphatase with EF calcium-binding domain) (PPEF) (Serine/threonine protein phosphatase 7) (PP7)
102.870e+00411 (9)16.09.7-6.2671.2 82286/8.6HUMANDNA replication licensing factor MCM5 (CDC46 homolog) (P1-CDC46)
46726.xx...........xx............x............x..x......x......x.........x...x.x.x..............x...........x..x......
100557............xxx...........x..x...............x.x.....x...............................x.x....................x.x..
102130.............................x.....x......................................x..........x.............xx..x.x.......
28533..........................................................xx.............x.............x........x.......x..x.....
109841............................x.x.........x....................x....................x....................x...x.....
55966..............x...........................x......................x...x...x...............x..x.....x..............
65207........................................x......x..................x.x........x..x.xx....................x........
81068................x.........x............x............x.x.....x....x........x...........................xx.........
82795.x..........x.....x..............x........x..........x......x.........................................x..x..x....
103156.........x..................................x..xxx...x..........x.....................x...........x.x..x.........


Detailed Results

1. 16/113 matches (14%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1014.48811014.47918.91Met-ox 181(-)MSRQFSSR(S)
1016.48221016.5013-193563640(K) (S)
1066.48431066.5169-312702771(K) (T)
1070.45071070.5019-48pyroGlu 4121(R)QFSSRSGYR(S)
1179.59371179.6010-6.23773860(K) (H)
1277.70711277.7105-2.64734830(K) (T)
1302.69261302.7018-7.03934031(R) (V)
1302.69261302.7157-183443550(R) (A)
1383.71821383.6909201861970(K) (V)
1475.76731475.7494122122230(K) (T)
1475.76731475.7858-132002110(R) (W)
1638.83191638.8604-171861991(K) (F)
1657.82811657.79342113290(R) (R)
1687.93441687.9707-2166821(R) (G)
1716.91431716.8517374184320(K) (G)
1994.00201993.9772122242390(R) (R)
2300.17012300.1985-123443641(R) (S)
2383.96552383.95245.55195490(R) (G)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 32% (207/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/113 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1060.54331060.5639-192242320(K) (M)
1065.50341065.49993.31541620(K) (L)
1066.48431066.4992-142422490(K) (Q)
1157.57711157.5915-122502600(R) (Q)
1190.60331190.6091-4.82612700(R) (S)
1307.66691307.6782-8.62402491(R) (Q)
1323.66871323.6731-3.31Met-ox 2402491(R)IKFEMEQNLR(Q)
1391.72831391.72830.0173273371(R) (K)
1837.95891837.9660-3.83743890(R) (K)
1867.95901867.9224201Met-ox 3213351(K)TLNDMRQEYEQLIAK(N)
2510.17912510.1323194494710(K) (I)
2705.15712705.1617-1.763940(R) (G)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 22% (137/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/113 matches (7%).
Acc. #: Species: MOUSE Name: Neural-cadherin precursor (N-cadherin) (Cadherin-2)
Index: MW: 99762 Da pI: 4.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1190.60331190.5615351Met-ox 1241330(R)EPTLTEEPMK(E)
1232.62221232.6639-346576670(R) (I)
1687.93441687.8768341101231(K) (E)
1837.95891837.9085278248410(R) (A)
2273.20652273.2069-0.176706900(K) (S)
2284.19142284.0879455065260(R) (Y)
2300.17012300.0829381Met-ox 5065260(R)QEEGLHAGTMLTTLTAQDPDR(Y)
2367.10252367.1230-8.74094301(K) (F)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 12% (117/906AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/113 matches (6%).
Acc. #: Species: HUMAN Name: Heat-shock protein 105 kDa (Heat shock 110 kDa protein) (Antigen NY-CO-25)
Index: MW: 96866 Da pI: 5.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1475.76731475.7342223613740(K) (G)
1479.66161479.7080-3120330(R) (C)
1661.81701661.7811226366481(K) (L)
1867.95901867.95880.127918060(K) (I)
2094.97222094.9411157547710(K) (K)
2342.99332343.0671-312762950(K) (D)
2451.19932451.1471217517711(K) (K)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 11% (98/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/113 matches (6%).
Acc. #: Species: HUMAN Name: Involucrin
Index: MW: 68469 Da pI: 4.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.59371179.6486-473954040(K) (H)
1193.60621193.5915123153240(K) (H)
1276.70631276.67242736460(K) (V)
1276.70631276.6802205765851(K) (-)
1487.75431487.68005069800(K) (E)
1790.90441790.9289-141501640(K) (H)
2300.17012300.16352.94424610(K) (N)
2451.19932451.2017-0.965025211(K) (H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 18% (108/585AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 8/113 matches (7%).
Acc. #: Species: HUMAN Name: Glutaminyl-tRNA synthetase (Glutamine--tRNA ligase) (GlnRS)
Index: MW: 87799 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1066.48431066.5277-411Met-ox 4044121(R)MKLVMEDGK(M)
1284.60111284.6258-191Met-ox 3954051(K)FSEGEATLRMK(L)
1549.77621549.7287317467590(R) (L)
1640.85631640.792039961090(R) (E)
1661.81701661.8346-1135500(R) (A)
1886.93511886.9410-3.16766900(K) (L)
2011.01522011.0422-131190(-) (A)
2225.12032225.1422-9.85395580(R) (D)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 14% (116/775AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 9/113 matches (7%).
Acc. #: Species: HUMAN Name: ADAM 2 precursor (A disintegrin and metalloproteinase domain 2) (Fertilin beta subunit) (PH-30) (PH30)
Index: MW: 82468 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1276.70631276.6611351Met-ox 1791880(K)YIEMHVIVEK(Q)
1323.66871323.6765-5.91041150(K) (G)
1566.76121566.7739-8.11Met-ox 6756860(R)YIENIYHSKPMR(W)
1638.83191638.7975211481611(K) (D)
1732.94361732.9672-1449630(K) (N)
1774.86291774.843411pyroGlu 1892040(K)QLYNHMGSDTTVVAQK(V)
1790.90441790.838337pyroGlu 1Met-ox 1892040(K)QLYNHMGSDTTVVAQK(V)
1791.86691791.8700-1.71892040(K) (V)
2342.99332342.9692106066241(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 13% (98/735AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 10/113 matches (8%).
Acc. #: Species: HUMAN Name: High-affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
Index: MW: 93305 Da pI: 5.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1072.50761072.5097-2.0pyroGlu 1371450(R)QLDAEALCR(S)
1157.57711157.5631122852930(K) (K)
1255.63671255.6898-422592691(K) (A)
1387.69421387.6462351Met-ox 1120(-)MGCAPSIHISER(L)
1404.69971404.7157-112702810(K) (I)
1485.75491485.7888-237577690(K) (N)
1549.77621549.7970-136596720(R) (H)
1687.93441687.8702383183311(R) (A)
2297.18262297.18110.664674870(K) (I)
2300.17012300.1895-8.47577761(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (122/829AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 10/113 matches (8%).
Acc. #: Species: HUMAN Name: Serine/threonine protein phosphatase with EF-hands-1 (PPEF-1) (Protein phosphatase with EF calcium-binding domain) (PPEF) (Serine/threonine protein phosphatase 7) (PP7)
Index: MW: 75793 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1014.48811014.5121-244914980(R) (K)
1060.54331060.5468-3.24484550(R) (A)
1107.56711107.5356284664750(R) (I)
1215.58151215.5462291111(-) (R)
1284.60111284.6112-7.96236330(K) (A)
1391.72831391.6953244644751(R) (I)
1485.75491485.8099-373053171(K) (D)
2297.18262297.1488156146331(K) (A)
2367.10252367.1807-336346531(K) (-)
2510.17912510.3005-481661861(K) (N)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 17% (113/653AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 11/113 matches (9%).
Acc. #: Species: HUMAN Name: DNA replication licensing factor MCM5 (CDC46 homolog) (P1-CDC46)
Index: MW: 82286 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1047.44451047.4967-501421500(K) (I)
1302.69261302.6476355705811(K) (L)
1323.66871323.66195.23383500(K) (K)
1334.62481334.6891-482522621(R) (V)
1365.65281365.6949-314824940(R) (W)
1391.72831391.7027181Met-ox 1391501(R)SLKSDMMSHLVK(I)
1515.75701515.8171-406836960(R) (D)
1838.92721838.8673333093241(R) (L)
2225.12032225.2150-431Met-ox 1421621(K)SDMMSHLVKIPGIIIAASAVR(A)
2284.19142284.1243294524711(R) (A)
2300.17012300.1192221Met-ox 4524711(R)EDDRVAIHEAMEQQTISIAK(A)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 16% (123/734AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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