MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D130
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 2033 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/126(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.257e+00814 (11)29.011.16.7338.1 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
24.385e+00510 (7)23.07.93.4168.9 59556/5.4MOUSET-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
32.624e+00510 (7)22.07.91.6367.4 59621/5.4HUMANT-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
46.051e+00411 (8)20.08.72.7543.3 73244/6.7HUMANDEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
53.406e+00413 (10)12.010.3-2.3155.6 94625/6.7HUMANProto-oncogene tyrosine-protein kinase FER (p94-FER) (c-FER)
62.257e+0049 (7)22.07.18.3452.5 63174/5.4HUMANComplement component C9 precursor
72.126e+00410 (7)9.07.9-4.1174.7 88484/8.9HUMANAFG3-like protein 2 (Paraplegin-like protein)
81.887e+00410 (7)19.07.92.5145.4 73102/6.7MOUSEDEAD-box protein 3 (DEAD-box RNA helicase DEAD3) (mDEAD3) (Embryonic RNA helicase) (D1PAS1 related sequence 2)
91.548e+0047 (5)15.05.6-1.1459.9 51578/6.5MOUSEInterferon regulatory factor 4 (IRF-4) (Lymphocyte specific interferon regulatory factor) (LSIRF) (NF-EM5) (PU.1 interaction partner) (Transcriptional activator PIP)
101.345e+00410 (7)11.07.9-14.855.0 84032/5.6HUMANHyaluronan mediated motility receptor (Intracellular hyaluronic acid binding protein) (Receptor for hyaluronan-mediated motility) (CD168 antigen)
46726.......x.........................x......x....x.......................x.....x.x.......x...x....xx.............x..x......x......
87118....................................................x..............x..........x...x................xxx..x..............xx.....
63957....................................................x..............x...x......x...xx...............x.x..x..............x......
3478.......................x...................x.............x......x.x....x..x....x................x...x.......x.................
28762......x.xx.....x.....x.x..............x.......x..................x.............x.......................xx........x............
24934.....x............................................................x..........x..............x...x.......x..xx.........x.......
80896....x.................x..x..x...xx.........x............x...........................x............x............................
67354.......................x...................x....................x.x....x..x....x................x...x.......x.................
32694.....x............................................................x..........x......x.......x.............x............x......
67188....................x......x.x....x.......x........x....x........x.................x........................x.................


Detailed Results

1. 14/126 matches (11%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
910.3934910.4158-252702760(K) (R)
973.5247973.5318-7.33964030(K) (V)
1015.43731015.4406-3.31Met-ox 2902980(K)DVDGAYMTK(V)
1033.52091033.51664.24844920(R) (M)
1179.62771179.6010233773860(K) (H)
1277.74181277.7105244734830(K) (T)
1302.68361302.7018-143934031(R) (V)
1302.68361302.7157-253443550(R) (A)
1383.71611383.6909181861970(K) (V)
1475.79421475.7494302122230(K) (T)
1475.79421475.78585.72002110(R) (W)
1657.82631657.79342013290(R) (R)
1687.95111687.9707-1266821(R) (G)
1994.01711993.9772202242390(R) (R)
2150.10732150.0783142242401(R) (R)
2384.03382383.9524345195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 29% (187/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/126 matches (7%).
Acc. #: Species: MOUSE Name: T-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
Index: MW: 59556 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1065.50121065.5217-193914000(R) (V)
1169.50211169.5512-422612700(K) (G)
1307.68981307.63843921310(K) (N)
1365.64941365.5956393793900(R) (A)
1797.00941796.9468351Met-ox 2552701(K)GTVLIKTAEELMNFSK(G)
1838.93571838.9574-121211370(R) (K)
1851.96831851.90233638541(K) (M)
1890.98131891.0475-355215390(R) (K)
2384.03382384.1114-332612811(K) (A)
2543.37872543.3139252712961(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (128/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/126 matches (7%).
Acc. #: Species: HUMAN Name: T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
Index: MW: 59621 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1065.50121065.5217-193914000(R) (V)
1169.50211169.5512-422612700(K) (G)
1232.59391232.6098-132973070(K) (Y)
1307.68981307.63843921310(K) (N)
1365.64941365.5956393793900(R) (A)
1372.75571372.74368.82822960(K) (V)
1797.00941796.9468351Met-ox 2552701(K)GTVLIKTAEELMNFSK(G)
1851.96831851.90233638541(K) (M)
1890.98131891.0475-355215390(R) (K)
2384.03382384.1114-332612811(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (122/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 11/126 matches (8%).
Acc. #: Species: HUMAN Name: DEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
Index: MW: 73244 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
940.3999940.4012-1.456640(K) (D)
1029.54681029.5118344814880(R) (S)
1090.54791090.54215.44194270(K) (R)
1157.61651157.5876253643730(R) (G)
1165.62861165.6594-2638461(R) (E)
1232.59391232.55483265751(K) (S)
1232.59391232.5812105936031(R) (Q)
1251.68291251.6836-0.584414510(K) (K)
1320.67531320.6800-3.52772870(R) (K)
1707.83021707.8964-393123261(R) (L)
1838.93571838.9513-8.55325481(R) (N)
1940.99061940.97925.93363511(K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (137/662AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 13/126 matches (10%).
Acc. #: Species: HUMAN Name: Proto-oncogene tyrosine-protein kinase FER (p94-FER) (c-FER)
Index: MW: 94625 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
908.4117908.4221-112142200(K) (A)
912.3999912.4249-277007070(K) (Q)
913.4060913.4420-395725790(K) (G)
924.4302924.4171141Met-ox 2142200(K)MQEEMIK(A)
936.4277936.4461-201461531(K) (Y)
940.3999940.4120-132Met-ox 2142200(K)MQEEMIK(A)
1007.53221007.5559-245865941(K) (E)
1036.55541036.5176367717781(R) (M)
1160.59461160.5985-3.4pyroGlu 1Met-ox 1421511(R)QLIKEMNSAK(E)
1320.67531320.7163-313773871(K) (V)
1874.06581873.9846431121271(K) (V)
1890.98131890.96697.63113260(K) (E)
2167.14392167.0640376897071(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (106/822AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 9/126 matches (7%).
Acc. #: Species: HUMAN Name: Complement component C9 precursor
Index: MW: 63174 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
907.4320907.39444178850(R) (R)
1165.62861165.62175.92322420(K) (F)
1302.68361302.6654141441541(R) (T)
1583.83741583.8069194975091(R) (C)
1707.83021707.8594-172772901(K) (E)
1890.98131891.0078-141952101(R) (N)
1934.90181934.8588222512670(K) (G)
1940.99061940.9143392112251(K) (S)
2369.16392369.2492-363123321(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (126/559AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 10/126 matches (7%).
Acc. #: Species: HUMAN Name: AFG3-like protein 2 (Paraplegin-like protein)
Index: MW: 88484 Da pI: 8.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
905.3928905.4369-49pyroGlu 3323390(K)QYQDLGAK(I)
939.3912939.4214-326256320(R) (A)
945.4157945.4318-171341410(K) (D)
955.4526955.4164381Met-ox 6256320(R)MCMTLGGR(A)
971.4309971.4113202Met-ox 6256320(R)MCMTLGGR(A)
973.5247973.4824431Met-ox 171(-)MAHRCLR(L)
1029.54681029.5118345445510(K) (V)
1089.50941089.5540-416426510(R) (K)
1382.67231382.7394-493183280(K) (N)
1715.89731715.8791117397530(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (75/797AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 10/126 matches (7%).
Acc. #: Species: MOUSE Name: DEAD-box protein 3 (DEAD-box RNA helicase DEAD3) (mDEAD3) (Embryonic RNA helicase) (D1PAS1 related sequence 2)
Index: MW: 73102 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
940.3999940.4012-1.456640(K) (D)
1029.54681029.5118344814880(R) (S)
1157.61651157.5876253643730(R) (G)
1165.62861165.6594-2638461(R) (E)
1232.59391232.55483265751(K) (G)
1232.59391232.5812105936031(R) (Q)
1251.68291251.6836-0.584414510(K) (K)
1320.67531320.6800-3.52772870(R) (K)
1707.83021707.8964-393123261(R) (L)
1838.93571838.9513-8.55325481(R) (N)
1940.99061940.97925.93363511(K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (128/662AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 7/126 matches (5%).
Acc. #: Species: MOUSE Name: Interferon regulatory factor 4 (IRF-4) (Lymphocyte specific interferon regulatory factor) (LSIRF) (NF-EM5) (PU.1 interaction partner) (Transcriptional activator PIP)
Index: MW: 51578 Da pI: 6.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
907.4320907.43071.4180(-) (G)
1165.62861165.5741471141230(R) (V)
1302.68361302.66941126360(R) (Y)
1382.67231382.6956-1783940(R) (T)
1583.83741583.8069191141261(R) (I)
1918.88951918.9485-313343490(R) (L)
2384.03382384.1258-391041231(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (70/450AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 10/126 matches (7%).
Acc. #: Species: HUMAN Name: Hyaluronan mediated motility receptor (Intracellular hyaluronic acid binding protein) (Receptor for hyaluronan-mediated motility) (CD168 antigen)
Index: MW: 84032 Da pI: 5.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
934.4246934.390636pyroGlu 5465520(K)QQEEDFR(K)
951.4136951.4172-3.85465520(K) (K)
958.4112958.4118-0.59pyroGlu 5545610(K)QLEDEEGR(K)
975.4027975.4383-375545610(K) (K)
1028.50391028.5376-333513590(K) (L)
1062.51501062.485628pyroGlu 5465531(K)QQEEDFRK(Q)
1062.51501062.5584-413253320(K) (E)
1089.50941089.5540-414014091(K) (A)
1160.59461160.6210-231Met-ox 1151241(K)MEARLNAALR(E)
1372.75571372.7688-9.52732840(K) (Q)
1940.99061941.0657-393353501(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (80/724AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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