MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.07.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D131
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 114561
Molecular weight search (1000 - 100000 Da) selects 108217 entries.
Full pI range: 114561 entries.
Species search ( HUMAN MOUSE ) selects 14035 entries.
Combined molecular weight, pI and species searches select 12424 entries.
Pre searches select 12424 entries.

Data Set 1 Results

MS-Fit search selects 124 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/72(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	5271	6 (8)	14.0	8.3	-54.1	30.7		86984/6.5		HUMAN	Cullin homolog 2 (CUL-2)
2	4598	5 (6)	16.0	6.9	-56.6	68.9		61988/5.1		HUMAN	Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
3	3031	5 (6)	12.0	6.9	-24.4	37.4		63036/6.0		HUMAN	Nuclear receptor ROR-alpha (Nuclear receptor RZR-alpha)
4	1763	4 (5)	11.0	5.6	-27.3	69.7		52889/7.5		HUMAN	RING finger protein 18 (Testis-specific ring-finger protein)
5	1272	4 (5)	10.0	5.6	-68.8	40.2		71289/6.0		MOUSE	GTP-RHO BINDING PROTEIN 1 (RHOPHILIN)

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103665	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 6/72 matches (8%).
Acc. #: Species: HUMAN Name: Cullin homolog 2 (CUL-2)
Index: MW: 86984 Da pI: 6.5

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1628.7241	1628.8318	-66		616	629	1	(K) (E)
1962.7587	1962.8721	-58	pyroGlu	729	745	1	(K)QYIERSQASADEYSYVA(-)
1962.7587	1962.9172	-81		536	551	0	(K) (K)
2193.9472	2194.0306	-38	2Met-ox	442	460	1	(K)RLIHGLSMSMDSEEAMINK(L)
2284.9818	2285.0735	-40		97	114	1	(K) (K)
2595.1797	2595.3102	-50		303	325	0	(R) (A)
2611.1865	2611.3051	-45	1Met-ox	303	325	0	(R)AVSTGLPHMIQELQNHIHDEGLR(A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (107/745AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/72 matches (6%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1605.7261 1605.7437 -11 199 211 0 (K) (D)
1791.6604 1791.7283 -38 490 512 0 (R) (G)
1851.7451 1851.9275 -98 321 335 1 (K) (N)
2298.9932 2299.1202 -55 271 289 1 (K) (N)
2704.9445 2705.1617 -80 63 94 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (102/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/72 matches (6%).
Acc. #: Species: HUMAN Name: Nuclear receptor ROR-alpha (Nuclear receptor RZR-alpha)
Index: MW: 63036 Da pI: 6.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1605.7261 1605.7185 4.7 404 417 0 (R) (Y)
1895.8012 1895.8890 -46 426 441 0 (K) (S)
2283.0080 2283.0572 -22 108 127 1 (K) (G)
2595.1797 2595.2621 -32 91 112 1 (K) (S)
2611.1865 2611.2570 -27 1Met-ox 91 112 1 (K)EVQTGYMNAQIEIIPCKICGDK(S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (67/556AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/72 matches (5%).
Acc. #: Species: HUMAN Name: RING finger protein 18 (Testis-specific ring-finger protein)
Index: MW: 52889 Da pI: 7.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1702.6623 1702.7794 -69 144 156 1 (K) (N)
2193.9472 2194.0425 -43 80 97 0 (K) (E)
2283.0080 2282.9997 3.6 308 326 0 (R) (S)
2298.9932 2298.9946 -0.61 1Met-ox 308 326 0 (R)SMCIGCDHQDVPYFTATPR(S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (50/452AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/72 matches (5%).
Acc. #: Species: MOUSE Name: GTP-RHO BINDING PROTEIN 1 (RHOPHILIN)
Index: MW: 71289 Da pI: 6.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1614.7112 1614.8280 -72 341 354 0 (R) (A)
1628.7241 1628.8284 -64 478 492 0 (K) (N)
1851.7451 1851.8289 -45 462 477 0 (R) (T)
2210.9258 2211.1331 -94 564 584 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (66/643AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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