MS-Fit Search Results



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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D132
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 3147 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score
#/131(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
15.692e+00711 (8)35.08.464.140.6 47232/4.9HUMANKeratin, type I cuticular HA1 (Hair keratin, type I HA1)
27.578e+00610 (7)32.07.655.765.8 46214/4.8HUMANKeratin, type I cuticular HA3-II (Hair keratin, type I HA3-II)
37.280e+00613 (9)25.09.918.091.6 80642/6.6HUMANProtein-tyrosine phosphatase epsilon precursor (R-PTP-epsilon)
42.320e+00611 (8)22.08.451.383.9 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
52.233e+00611 (8)35.08.472.847.6 36054/8.6HUMANGlyceraldehyde 3-phosphate dehydrogenase, liver
64.484e+00512 (9)18.09.214.094.5 82642/9.4HUMANATP-binding cassette, sub-family B, member 7, mitochondrial precursor (ATP-binding cassette transporter 7) (ABC transporter 7 protein)
74.004e+00510 (7)20.07.628.676.5 77867/8.9MOUSELactotransferrin precursor (Lactoferrin)
82.877e+00513 (9)23.09.949.865.6 77009/6.7MOUSEGlucosamine--fructose-6-phosphate aminotransferase [isomerizing] 2 (Hexosephosphate aminotransferase 2) (D-fructose-6-phosphate amidotransferase 2) (GFAT 2) (GFAT2)
92.567e+00512 (9)24.09.251.4101 85459/9.0HUMANZinc finger protein 84 (Zinc finger protein HPF2)
102.429e+0058 (6)27.06.178.318.5 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
96541.....................x.x.....................x.x..........x...............x...x......................x.........x...x....x..........
52875................x....x.........x.............x.xx.........................x..............................x.....x........x..........
84524...............x....x..x................x.x..........x...x...x...x.........................x...x....................x........x.....
45313..x...................x......x.x......x...x..................x................x.....x........x......................x..............
108737...................................................x...x...........x..................x............x.x.x.....x.....x.....x.x.......
74500................x.x.x...........x......x.....x..............x.........................................x....xx.x..............x.....
24431....................x...........x.......x...............................x....x.......x......x...........x..x.........x.............
21403x...........x.................xx...............x...................x..x..............x.....x..x.......x..........x...........x.....
1578x.......x..........................................x..x...x.......x..xx.x...................................x......x.x.............
97443..................................x......................................x.x...................x.....................x......x...xx.



Detailed Results

1. 11/131 matches (8%).
Acc. #: Species: HUMAN Name: Keratin, type I cuticular HA1 (Hair keratin, type I HA1)
Index: MW: 47232 Da pI: 4.9
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1169.62201169.5261821Met-ox 58660(K)ETMQFLNDR(L)
1218.65341218.6218261741840(K) (E)
1487.85451487.749471pyroGlu 3353460(R)QNQEYQVLLDVR(A)
1504.89631504.7760803353460(R) (A)
1625.96731625.8499722092230(R) (V)
1849.14381848.9932812782930(R) (D)
1882.02111881.8870712422560(R) (Q)
2229.27352229.1628501451631(K) (R)
2307.35152307.1832732572760(K) (R)
2369.25452369.1883282032231(R) (V)
2593.53122593.3473713073290(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (146/416AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/131 matches (7%).
Acc. #: Species: HUMAN Name: Keratin, type I cuticular HA3-II (Hair keratin, type I HA3-II)
Index: MW: 46214 Da pI: 4.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1094.49321094.5040-9.83583660(R) (L)
1169.62201169.5261821Met-ox 58660(K)ETMQFLNDR(L)
1302.73241302.72049.21641731(R) (S)
1487.85451487.749471pyroGlu 3353460(R)QNQEYQVLLDVR(A)
1504.89631504.7760803353460(R) (A)
1512.76881512.7182332943060(R) (Y)
1849.14381848.9932812782930(R) (Y)
2280.33392280.1836662092290(R) (N)
2307.35152307.1832732572760(K) (R)
2593.53122593.3473713073290(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (133/404AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 13/131 matches (9%).
Acc. #: Species: HUMAN Name: Protein-tyrosine phosphatase epsilon precursor (R-PTP-epsilon)
Index: MW: 80642 Da pI: 6.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1089.49581089.5727-711Met-ox 4454541(R)TGNLPANMKK(A)
1158.62251158.6557-296066160(K) (Q)
1218.65341218.6330175575670(K) (D)
1434.79931434.79572.56376490(R) (V)
1475.87051475.8256302272391(K) (K)
1595.82171595.8043111631751(K) (V)
1618.91321618.8085652762881(R) (A)
1699.97601699.84377885981(K) (V)
1715.94401715.8387611Met-ox 85981(K)KMPNGILEEQEQQR(V)
2125.15612124.9954763423600(R) (V)
2171.19332171.1209335685851(R) (V)
2384.14712384.1531-2.56676840(R) (V)
2717.31452717.4165-382923150(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (177/700AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 11/131 matches (8%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
973.4725973.5318-613964030(K) (V)
1179.68491179.6010713773860(K) (H)
1277.77831277.7105534734830(K) (T)
1302.73241302.7018243934031(R) (V)
1302.73241302.7157133443550(R) (A)
1383.79541383.6909761861970(K) (V)
1475.87051475.7494822122230(K) (T)
1475.87051475.7858572002110(R) (W)
1699.97601699.825189pyroGlu 4184320(K)QISNLQQSISDAEQR(G)
1882.02111881.9711271821971(R) (V)
1994.13611993.9772802242390(R) (R)
2150.23992150.0783752242401(R) (R)
2384.14712383.9524825195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (147/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 11/131 matches (8%).
Acc. #: Species: HUMAN Name: Glyceraldehyde 3-phosphate dehydrogenase, liver
Index: MW: 36054 Da pI: 8.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1544.92681544.8107752352480(R) (L)
1614.01311613.90156967800(K) (D)
1763.93961763.8029773103230(K) (V)
2041.30482041.10829667841(K) (I)
2211.30632211.2171402352541(R) (Y)
2229.27352229.1048761Met-ox 1191390(R)VIISAPSADAPMFVMGVNHEK(Y)
2245.29992245.0997892Met-ox 1191390(R)VIISAPSADAPMFVMGVNHEK(Y)
2293.24132293.0335911Met-ox 871070(K)WGDAGAEYVVESTGVFTTMEK(A)
2369.25452369.2110181181391(K) (Y)
2595.55342595.3605741631860(K) (T)
2611.60122611.3554941Met-ox 1631860(K)VIHDNFGIVEGLMTTVHAITATQK(T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (119/335AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 12/131 matches (9%).
Acc. #: Species: HUMAN Name: ATP-binding cassette, sub-family B, member 7, mitochondrial precursor (ATP-binding cassette transporter 7) (ABC transporter 7 protein)
Index: MW: 82642 Da pI: 9.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1094.49321094.5709-711231310(K) (D)
1107.54241107.5435-0.9572820(K) (A)
1158.62251158.6370-137367450(K) (G)
1303.79231303.7262514874990(K) (K)
1405.76411405.74401469821(R) (A)
1487.85451487.8546-0.0405015161(K) (L)
1636.97841636.8984491Met-ox 4364490(R)QALIDMNTLFTLLK(V)
2232.32562232.1210921201(-) (R)
2285.36382285.22945972921(K) (R)
2288.26222288.25762.0pyroGlu 4364551(R)QALIDMNTLFTLLKVDTQIK(D)
2305.37772305.2841414364551(R) (D)
2717.31452717.4640-552282511(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (136/752AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 10/131 matches (7%).
Acc. #: Species: MOUSE Name: Lactotransferrin precursor (Lactoferrin)
Index: MW: 77867 Da pI: 8.9
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1158.62251158.61595.72822900(K) (Q)
1303.79231303.737442921030(K) (E)
1434.79931434.7779155505621(R) (D)
1794.91901794.90607.26546681(K) (I)
1878.05401877.9471572472610(K) (E)
1998.12041998.2077-443200(R) (A)
2148.24702148.0694831Met-ox 4825001(R)TAGWNIPMGLLANQTRSCK(F)
2274.34092274.1665774384580(K) (R)
2285.36382285.34936.41201(-) (A)
2510.33592510.2435371181391(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (146/707AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 13/131 matches (9%).
Acc. #: Species: MOUSE Name: Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 2 (Hexosephosphate aminotransferase 2) (D-fructose-6-phosphate amidotransferase 2) (GFAT 2) (GFAT2)
Index: MW: 77009 Da pI: 6.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
968.5312968.4478866666730(R) (N)
1045.54311045.4777631Met-ox 3203280(K)GNFSAFMQK(E)
1300.66361300.6650-1.01881990(K) (R)
1302.73241302.6588566036130(K) (Q)
1504.89631504.8375393453580(R) (D)
1763.93961763.8435541141(-) (T)
1779.94621779.8385611Met-ox 1141(-)MCGIFAYMNYRVPK(T)
1998.12041998.10249.03453621(R) (E)
2125.15612125.0354572552730(K) (V)
2168.30172168.1645633Met-ox 5785961(K)HGPLALVDKQMPVIMVIMK(D)
2232.32562232.1586752142320(K) (N)
2358.31112358.0820972Met-ox 4784970(K)AYTSQFISLVMFGLMMSEDR(I)
2717.31452717.3465-122Met-ox 3063281(R)AIQTLQMELQQIMKGNFSAFMQK(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (163/682AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 12/131 matches (9%).
Acc. #: Species: HUMAN Name: Zinc finger protein 84 (Zinc finger protein HPF2)
Index: MW: 85459 Da pI: 9.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
968.5312968.4624713513581(R) (K)
968.5312968.52783.55005070(K) (I)
1025.44401025.5281-822482550(K) (T)
1544.92681544.7934865795911(K) (I)
1606.01881605.8753891391521(K) (A)
1625.96731625.8335825235351(K) (T)
1761.90721761.9176-5.921341(K) (D)
1777.92561777.8365505926050(R) (K)
1779.94621779.8270673683821(R) (A)
1794.91901794.7903722562700(R) (A)
2288.26222288.0552903934101(R) (A)
2369.25452369.0681791200(-) (E)
2510.33592510.1709661Met-ox 1041241(R)GHECDAFGKNFNLNMNFVPLR(K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (181/738AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 8/131 matches (6%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1307.77311307.6782732402491(R) (Q)
1838.09631837.9660713743890(R) (K)
1852.09821851.9275923213351(K) (N)
2171.19332171.0252772712880(K) (K)
2510.33592510.1323814494710(K) (I)
2705.37352705.16177863940(R) (G)
2902.59782902.4111641992231(K) (T)
3264.80553264.5144893393670(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (168/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


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