MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D136
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 119 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/39(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	4.608e+004	9 (23)	26.0	23.1	-0.634	54.5		27566/6.2		HUMAN	Glutathione transferase omega 1 (GSTO 1-1)
2	3267	6 (15)	18.0	15.4	24.9	50.1		66018/8.2		HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
3	1138	5 (12)	4.0	12.8	-13.1	117		83201/5.3		MOUSE	Myosin Vb (Myosin 5B)
4	648	4 (10)	15.0	10.3	41.5	44.7		58629/9.1		MOUSE	T-box transcription factor TBX6 (T-box protein 6)
5	577	4 (10)	10.0	10.3	48.3	21.5		71289/6.0		MOUSE	GTP-RHO BINDING PROTEIN 1 (RHOPHILIN)

82320	.	.	.	.	x	.	.	.	x	.	x	x	x	x	.	.	x	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.
45313	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	x	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	x	.	.
112432	.	x	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.
61173	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	x	.	.	.	x	.
102436	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.

Detailed Results

1. 9/39 matches (23%).
Acc. #: Species: HUMAN Name: Glutathione transferase omega 1 (GSTO 1-1)
Index: MW: 27566 Da pI: 6.2

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
940.4098	940.4351	-27		31	37	0	(R) (T)
996.5420	996.5440	-2.0	1Met-ox	115	122	0	(K)MILELFSK(V)
1074.6263	1074.6312	-4.6		123	132	0	(K) (S)
1079.6006	1079.6213	-19		49	57	0	(R) (N)
1089.5028	1089.5468	-40		102	110	0	(K) (A)
1095.5577	1095.5627	-4.6		58	65	0	(K) (K)
1320.7131	1320.6912	17		12	25	0	(K) (I)
2036.2284	2036.1618	33		115	132	1	(K) (S)
2052.2443	2052.1567	43	1Met-ox	115	132	1	(K)MILELFSKVPSLVGSFIR(S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (65/241AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/39 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1127.5306 1127.5407 -8.9 289 298 1 (K) (V)
1475.7834 1475.7494 23 212 223 0 (K) (T)
1475.7834 1475.7858 -1.6 200 211 0 (R) (W)
1813.9358 1813.8945 23 13 30 1 (R) (T)
1942.0431 1941.9882 28 444 460 1 (K) (L)
1994.0768 1993.9772 50 224 239 0 (R) (R)
2384.0969 2383.9524 61 519 549 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (116/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/39 matches (12%).
Acc. #: Species: MOUSE Name: Myosin Vb (Myosin 5B)
Index: MW: 83201 Da pI: 5.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
925.3541 925.4202 -71 pyroGlu 464 470 0 (K)QNEHCLK(N)
939.3798 939.4284 -52 5 12 0 (R) (K)
942.4067 942.4467 -42 464 470 0 (K) (N)
2036.2284 2036.1367 45 559 575 0 (K) (K)
2052.2443 2052.1316 55 1Met-ox 559 575 0 (K)QLFYMINAVTLNNLLLR(K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 4% (32/723AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/39 matches (10%).
Acc. #: Species: MOUSE Name: T-box transcription factor TBX6 (T-box protein 6)
Index: MW: 58629 Da pI: 9.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
2211.2175 2211.0835 61 17 37 0 (R) (Y)
2283.2926 2283.2171 33 373 393 0 (R) (G)
2284.2956 2284.1616 59 394 413 0 (R) (G)
2705.3532 2705.3159 14 1Met-ox 172 194 1 (R)VYIHPDSPATGAHWMRQPVSFHR(V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (85/540AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/39 matches (10%).
Acc. #: Species: MOUSE Name: GTP-RHO BINDING PROTEIN 1 (RHOPHILIN)
Index: MW: 71289 Da pI: 6.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1338.6522 1338.5822 52 599 609 0 (R) (T)
1838.9907 1838.9149 41 59 74 1 (R) (E)
2036.2284 2036.1028 62 516 537 0 (R) (E)
2211.2175 2211.1331 38 564 584 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (70/643AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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