MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D139
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 214 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/37(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.235e+0045 (13)12.013.59.99103 72259/5.9MOUSEHistidine ammonia-lyase (Histidase)
21.063e+0047 (18)35.018.9-16.683.4 41426/5.6HUMANGuanine nucleotide-binding protein G(q), alpha subunit
360826 (16)16.016.215.357.0 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
441945 (13)15.013.555.942.9 58629/9.1MOUSET-box transcription factor TBX6 (T-box protein 6)
541486 (16)17.016.231.160.6 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
633576 (16)30.016.2-16.290.1 41479/5.7MOUSEGuanine nucleotide-binding protein G(q), alpha subunit
724405 (13)11.013.536.7110 62244/7.7HUMANSH3 domain-binding protein 2 (3BP-2)
818868 (21)10.021.631.059.6 95203/8.9MOUSEGlutamate receptor, ionotropic kainate 1 precursor (Glutamate receptor 5) (GluR-5) (GluR5)
918377 (18)15.018.9-9.5587.3 73141/6.7MOUSEPutative ATP-dependent RNA helicase PL10
1015856 (16)13.016.2-5.8760.8 65866/8.1HUMANKeratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
17841.......................x.xx.x.x......
26652.......x...x...x.......x..x.....x...x
45313.......x.x...xx.x.................x..
61173....................x.......x..xx..x.
97443..........x.x......xx.x............x.
54381.......x...x...x..........x.....x...x
49332..............x......x.x..x.x........
4713.....x........x.....xxxx...x.x.......
98092.........x.x.....xx..x.xx............
110808......x..x.x..x......x............x..


Detailed Results

1. 5/37 matches (13%).
Acc. #: Species: MOUSE Name: Histidine ammonia-lyase (Histidase)
Index: MW: 72259 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1941.01781941.0446-144524690(K) (R)
2097.03822097.1457-514524701(K) (I)
2195.01482195.0654-234164350(K) (G)
2211.22752211.0603761Met-ox 4164350(K)DIITTELNSATDNPMVFASR(G)
2233.22802233.0750693683861(R) (F)
2233.22802233.21923.92492720(K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (82/657AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/37 matches (18%).
Acc. #: Species: HUMAN Name: Guanine nucleotide-binding protein G(q), alpha subunit
Index: MW: 41426 Da pI: 5.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.58161179.6448-5487961(R) (Y)
1320.60011320.6072-5.455660(R) (R)
1493.74721493.672850AcetN 1131(-)ACCLSEEAKEAR(R)
1493.74721493.8076-401021141(K) (V)
1941.01781941.0558-201611771(R) (V)
2195.01482195.1752-731781960(R) (M)
2284.31362284.2382332512690(R) (K)
2717.31252717.3761-233173391(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (126/353AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/37 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.58161179.6010-163773860(K) (H)
1300.55181300.5302172582670(K) (N)
1383.68761383.6909-2.41861970(K) (V)
1475.77811475.7494192122230(K) (T)
1475.77811475.7858-5.22002110(R) (W)
1688.01251687.97072566821(R) (G)
2384.11982383.9524705195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (104/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/37 matches (13%).
Acc. #: Species: MOUSE Name: T-box transcription factor TBX6 (T-box protein 6)
Index: MW: 58629 Da pI: 9.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1838.02461837.8808781721870(R) (Q)
2211.22752211.08356517370(R) (Y)
2283.32132283.2171463733930(R) (G)
2284.31362284.1616673944130(R) (G)
2705.38022705.3159241Met-ox 1721941(R)VYIHPDSPATGAHWMRQPVSFHR(V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (85/540AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/37 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1307.67801307.6782-0.172402491(R) (Q)
1350.68651350.6878-1.04724861(K) (G)
1791.78901791.7283344905120(R) (G)
1838.02461837.9660323743890(R) (K)
1852.00351851.9275413213351(K) (N)
2705.38022705.16178163940(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (111/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 6/37 matches (16%).
Acc. #: Species: MOUSE Name: Guanine nucleotide-binding protein G(q), alpha subunit
Index: MW: 41479 Da pI: 5.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.58161179.6448-5487961(R) (Y)
1320.60011320.6072-5.455660(R) (R)
1493.74721493.672850AcetN 1131(-)ACCLSEEAKEAR(R)
1493.74721493.8076-401021141(K) (V)
2195.01482195.1752-731781960(R) (M)
2284.31362284.2382332512690(R) (K)
2717.31252717.3761-233173391(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (109/353AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 5/37 matches (13%).
Acc. #: Species: HUMAN Name: SH3 domain-binding protein 2 (3BP-2)
Index: MW: 62244 Da pI: 7.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1475.77811475.6443911071180(R) (K)
1838.99571838.8707684784931(R) (S)
1941.01781940.95403381971(R) (I)
2195.01482195.1323-541942130(R) (K)
2211.22752211.1272451Met-ox 1942130(R)LEDALMHPPAYPPPPVPTPR(K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (65/561AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 8/37 matches (21%).
Acc. #: Species: MOUSE Name: Glutamate receptor, ionotropic kainate 1 precursor (Glutamate receptor 5) (GluR-5) (GluR5)
Index: MW: 95203 Da pI: 8.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1045.53391045.52785.87517591(K) (S)
1475.77811475.7203394204321(R) (T)
1838.02461837.8795791Met-ox 6806951(K)IEYGAVRDGSTMTFFK(K)
1838.99571838.9396307868010(K) (N)
1852.00351851.9228443874021(K) (I)
1941.01781941.0407-124334480(R) (K)
2209.14982209.13616.27868041(K) (K)
2225.25472225.1310561Met-ox 7868041(K)CLSFNAIMEELGISLKNQK(K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (89/836AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 7/37 matches (18%).
Acc. #: Species: MOUSE Name: Putative ATP-dependent RNA helicase PL10
Index: MW: 73141 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1300.55181300.6398-684784871(R) (S)
1320.60011320.6800-602762860(R) (K)
1707.83381707.8964-373113251(R) (L)
1765.81401765.7741235996151(R) (A)
1838.99571838.9513245315471(R) (N)
1941.01781940.9792203353501(K) (M)
2083.09032083.0249315115270(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (103/660AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 6/37 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
Index: MW: 65866 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1107.51781107.5435-233603680(K) (S)
1300.55181300.5812-231Met-ox 1101(-)MSCQISCKSR(G)
1320.60011320.58341346610(R) (S)
1475.77811475.7858-5.22042150(R) (W)
1838.99571838.91494471920(K) (G)
2384.11982384.2172-414684871(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (89/645AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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