MS-Fit Search Results



.

Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D142
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 3402 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score
#/111(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.384e+01220 (18)32.018.069.653.3 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
28.373e+00616 (14)35.014.478.654.4 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
36.407e+00613 (11)25.011.745.4106 67198/9.7HUMANSpastin
42.883e+00612 (10)21.010.851.177.6 80642/6.6HUMANProtein-tyrosine phosphatase epsilon precursor (R-PTP-epsilon)
52.304e+00618 (16)24.016.255.784.9 96821/5.5HUMANTuftelin-interacting protein 11 (HSPC006)
65.456e+00514 (12)23.012.640.982.5 75483/9.4MOUSEATP-binding cassette, sub-family D, member 3 (70 kDa peroxisomal membrane protein) (PMP70) (PMP68)
75.017e+00515 (13)22.013.530.5107 75477/9.4HUMANATP-binding cassette, sub-family D, member 3 (70 kDa peroxisomal membrane protein) (PMP70)
81.912e+00510 (9)19.09.062.147.3 72038/5.8MOUSEINTERFERON-INDUCED GTP-BINDING PROTEIN MX1 (INFLUENZA RESISTANCE PROTEIN)
91.707e+00511 (9)23.09.964.6103 55622/9.4MOUSESpastin
101.697e+00511 (9)14.09.980.263.6 86475/8.9HUMANPhosphate regulating neutral endopeptidase (Metalloendopeptidase homolog PEX) (X-linked hypophosphatemia protein) (HYP) (Vitamin D-resistant hypophosphatemic rickets protein)
45313...x......xx.x......x..xx.x.........xx...........x.....xxx.x.x..............xx......x.............x............
97443.....xx....x...x.........x.x...x.................................xxxx....x.x...................x.......x.x.....
10566..............x....x.......x.....x.x...........x...............x.x.....x....x..x..x.........x..................
84524............x..x......................x..x.......x....x....x...x....x..x..........................x.....x......
27058..x.......x........xx..xxxx..........x......x....x....xx........x.............x.............x..x......x........
109664.......x.........x.x....xx..x.x.............x..............x............xx......x.x...............x............
87357.x.....x.........x.x.....x.xx.x.......xx................................xx......x.x...............x............
88570...x............x........x.x..x..................xx....x......................x.....................x..........
37344......x.......xx.........x.x.....x.............x.................x.....x..........x.........x..................
35805.................x.x.........x..x.........xx...............x.....x.......x.x...x...............................



Detailed Results

1. 20/111 matches (18%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1033.54541033.5166284844920(R) (M)
1141.58771141.5199594644720(R) (L)
1157.64331157.51491111Met-ox 4644720(R)DYQELMNTK(L)
1179.66141179.6010513773860(K) (H)
1277.79401277.7105654734830(K) (T)
1300.61771300.5302672582670(K) (N)
1302.72481302.7018183934031(R) (V)
1302.72481302.71577.03443550(R) (A)
1316.62021316.5251721Met-ox 2582670(K)NMQDMVEDYR(N)
1383.78741383.6909701861970(K) (V)
1393.82001393.7327632782891(R) (D)
1475.85971475.7494752122230(K) (T)
1475.85971475.7858502002110(R) (W)
1523.89691523.7892714614721(R) (L)
1638.99291638.8604811861991(K) (F)
1657.94431657.79349113290(R) (R)
1699.97191699.825186pyroGlu 4184320(K)QISNLQQSISDAEQR(G)
1716.99771716.8517854184320(K) (G)
1986.09021985.8949982522671(R) (N)
1994.15731993.9772902242390(R) (R)
2150.26872150.0783892242401(R) (R)
2384.20062383.95241045195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (211/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 16/111 matches (14%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1060.60331060.5639372242320(K) (M)
1065.54741065.4999451541620(K) (L)
1157.64331157.5915452502600(R) (Q)
1206.72301206.6040991Met-ox 2612700(R)QVLDNLTMEK(S)
1206.72301206.6152893173261(K) (Q)
1307.75871307.6782622402491(R) (Q)
1323.76091323.6731661Met-ox 2402491(R)IKFEMEQNLR(Q)
1350.72191350.6878254724861(K) (G)
1791.93411791.72831154905120(R) (G)
1838.14251837.9660963743890(R) (K)
1852.08611851.9275863213351(K) (N)
1868.07231867.9224801Met-ox 3213351(K)TLNDMRQEYEQLIAK(N)
1955.12391954.9544871Met-ox 2242391(K)TLLDIDNTRMTLDDFR(I)
1966.25441966.0609983743901(R) (A)
2299.32612299.1202902712891(K) (N)
2705.45882705.161711063940(R) (G)
3223.62603223.28211075796180(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (221/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 13/111 matches (11%).
Acc. #: Species: HUMAN Name: Spastin
Index: MW: 67198 Da pI: 9.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1182.67041182.54311086076161(R) (-)
1274.71111274.6705322983090(R) (K)
1323.76091323.6657722222340(R) (R)
1363.65841363.7222-475956060(R) (W)
1382.76261382.6990462662790(R) (Q)
1462.89521462.7654895035141(K) (L)
1771.03031771.01926.31922061(R) (S)
1791.93411791.9394-2.95956091(R) (D)
1899.11211898.87721244634790(K) (V)
1986.09021986.1152-1366810(R) (F)
2083.11372083.10573.849650(R) (L)
2140.24822140.0562904614791(R) (V)
2283.41442283.2243831Met-ox 4804991(R)VLVMGATNRPQELDEAVLRR(F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (160/616AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 12/111 matches (10%).
Acc. #: Species: HUMAN Name: Protein-tyrosine phosphatase epsilon precursor (R-PTP-epsilon)
Index: MW: 80642 Da pI: 6.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1158.63691158.6557-166066160(K) (Q)
1206.72301206.6768381Met-ox 2272370(K)SATIVMLTNLK(E)
1401.80661401.7490415685790(R) (Q)
1434.88801434.7957646376490(R) (V)
1475.85971475.70991024414531(K) (K)
1475.85971475.8256232272391(K) (K)
1507.87441507.69971162Met-ox 4414531(K)ENMRTGNLPANMK(K)
1699.97191699.84377585981(K) (V)
1771.03031770.9067701121250(K) (I)
1868.07231867.9298762612750(R) (K)
1899.11211899.0016581111251(K) (I)
2384.20062384.1531206676840(R) (V)
2717.40752717.4165-3.32923150(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (148/700AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 18/111 matches (16%).
Acc. #: Species: HUMAN Name: Tuftelin-interacting protein 11 (HSPC006)
Index: MW: 96821 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1022.51931022.50939.71Met-ox 180(-)MSLSHLYR(D)
1141.58771141.6118-214914990(R) (N)
1274.71111274.56871121Met-ox 3593680(K)MTEVLDHEER(V)
1277.79401277.7040701Met-ox 1561661(K)LLQKMGYVPGR(G)
1300.61771300.60956.34444540(K) (W)
1302.72481302.7422-135635731(R) (L)
1307.75871307.59751233763850(K) (R)
1316.62021316.5581477427510(K) (R)
1393.82001393.7150751Met-ox 7617740(R)GIGVAASSVPMNFK(D)
1447.86641447.8234301Met-ox 4214330(R)VDLAVAIVYPLMK(E)
1475.85971475.7873494804900(R) (N)
1475.85971475.7892482622741(K) (E)
1507.87441507.7327947147280(R) (E)
1523.89691523.72771111Met-ox 7147280(R)AVSSNVGAYMQPGAR(E)
1788.93321788.8954216887031(K) (F)
2016.13252016.0072621Met-ox 3593751(K)MTEVLDHEERVISNLSK(V)
2283.41442283.2059917757941(K) (R)
2299.32612299.2008541Met-ox 7757941(K)DLIETKAEEHNIVFMPVIGK(R)
2565.40072565.17598822411(R) (H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (209/837AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 14/111 matches (12%).
Acc. #: Species: MOUSE Name: ATP-binding cassette, sub-family D, member 3 (70 kDa peroxisomal membrane protein) (PMP70) (PMP68)
Index: MW: 75483 Da pI: 9.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1090.57451090.6182-401Met-ox 3913990(R)ITELMQVLK(D)
1258.73201258.6618561111(-) (N)
1274.71111274.6567431Met-ox 1111(-)MAAFSKYLTAR(N)
1302.72481302.7422-136166270(K) (C)
1307.75871307.6928502Met-ox 3633731(R)MLLRMSQALGR(I)
1328.83931328.69241113113220(R) (Y)
1344.75381344.6873491Met-ox 3113220(R)FSMGFIDSIIAK(Y)
1447.86641447.7765621Met-ox 2492601(R)RPIGKMTIMEQK(Y)
1699.97191699.9437172983101(R) (F)
1934.17001934.0652544804961(K) (L)
1955.12391955.0577345055200(K) (D)
2095.13852094.90791105585760(R) (Q)
2140.24822140.1741355035201(R) (D)
2384.20062384.18805.31751951(K) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (155/659AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 15/111 matches (13%).
Acc. #: Species: HUMAN Name: ATP-binding cassette, sub-family D, member 3 (70 kDa peroxisomal membrane protein) (PMP70)
Index: MW: 75477 Da pI: 9.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
972.5549972.6094-565355431(R) (E)
1090.57451090.6182-401Met-ox 3913990(R)ITELMQVLK(D)
1258.73201258.6618561111(-) (N)
1274.71111274.6567431Met-ox 1111(-)MAAFSKYLTAR(N)
1307.75871307.6928502Met-ox 3633731(R)MLLRMSQALGR(I)
1323.76091323.8550-7167771(R) (T)
1328.83931328.69241113113220(R) (Y)
1344.75381344.6873491Met-ox 3113220(R)FSMGFIDSIIAK(Y)
1401.80661401.7888132492601(R) (Y)
1417.87661417.7837661Met-ox 2492601(R)RPIGKMTITEQK(Y)
1934.17001934.0652544804961(K) (L)
1955.12391955.0577345055200(K) (D)
2095.13852094.90791105585760(R) (Q)
2140.24822140.1741355035201(R) (D)
2384.20062384.18805.31751951(K) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (150/659AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 10/111 matches (9%).
Acc. #: Species: MOUSE Name: INTERFERON-INDUCED GTP-BINDING PROTEIN MX1 (INFLUENZA RESISTANCE PROTEIN)
Index: MW: 72038 Da pI: 5.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1033.54541033.473769AcetN 190(-)DSVNNLCR(H)
1232.66771232.6309304304400(K) (R)
1307.75871307.6703685845940(K) (C)
1323.76091323.6652721Met-ox 5845940(K)MMLQLLQDTSK(C)
1344.75381344.6833522933030(R) (S)
1475.85971475.7858501121260(K) (L)
1475.85971475.7892481Met-ox 4284401(R)VKALEESAVNMLR(R)
1484.86401484.82592615260(K) (A)
1523.89691523.73821042712830(K) (A)
2016.13252015.9571874534690(K) (S)
2510.44822510.2561771Met-ox 3513721(R)NIMNLIQAQETVSEGDSRLFTK(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (123/631AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 11/111 matches (9%).
Acc. #: Species: MOUSE Name: Spastin
Index: MW: 55622 Da pI: 9.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1065.54741065.5190271431530(K) (A)
1182.67041182.54311084955041(R) (-)
1206.72301206.60061012292390(K) (Q)
1307.75871307.6708671251360(K) (S)
1323.76091323.6657721101220(R) (R)
1363.65841363.7222-474834940(R) (W)
1462.89521462.7654893914021(K) (L)
1791.93411791.9394-2.94834971(R) (D)
1899.11211898.87721243513670(K) (V)
2140.24822140.0562903493671(R) (V)
2283.41442283.2243831Met-ox 3683871(R)VLVMGATNRPQELDEAVLRR(F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (120/504AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 11/111 matches (9%).
Acc. #: Species: HUMAN Name: Phosphate regulating neutral endopeptidase (Metalloendopeptidase homolog PEX) (X-linked hypophosphatemia protein) (HYP) (Vitamin D-resistant hypophosphatemic rickets protein)
Index: MW: 86475 Da pI: 8.9
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1258.73201258.58121201371460(K) (A)
1274.71111274.57611061Met-ox 1371460(K)ILYSSCMNEK(A)
1329.78091329.6763796396510(R) (E)
1351.75611351.7156301Met-ox 3703801(R)MVYSRIPNLSR(R)
1438.79971438.62071241Met-ox 7307410(K)AFNCPPNSTMNR(G)
1445.81531445.7218655125220(K) (K)
1699.97191699.8399781371501(K) (A)
1791.93411791.7826855966100(K) (F)
1955.12391954.9663811671830(R) (K)
1966.25441966.0762913263421(R) (V)
2083.11372083.0613251671841(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (111/749AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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