MS-Fit Search Results



Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D143
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (10000 - 30000 Da) selects 41148 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 3825 entries.
Pre searches select 3825 entries.

Data Set 1 Results

MS-Fit search selects 92 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score
#/89(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
113215 (5)32.05.639.686.6 22965/6.1MOUSEProteasome subunit beta type 3 (Proteasome theta chain) (Proteasome chain 13) (Proteasome component C10-II)
212608 (8)43.09.05.26106 22088/5.4HUMANAdenylate kinase isoenzyme 5 (ATP-AMP transphosphorylase)
36954 (4)22.04.514.5135 28384/6.2MOUSEGlandular kallikrein K22 precursor (Tissue kallikrein) (MGK-22) (Epidermal growth factor-binding protein type A) (EGF-BP A) (Nerve growth factor beta chain endopeptidase) (Beta-NGF-endopeptidase)
45564 (4)23.04.536.598.6 22949/6.1HUMANProteasome subunit beta type 3 (Proteasome theta chain) (Proteasome chain 13) (Proteasome component C10-II)
55414 (4)20.04.550.520.2 29822/7.0HUMANHLA class II histocompatibility antigen, DR-7 beta chain precursor
63654 (4)11.04.546.769.7 23741/5.4MOUSEContactin 2 (Axonin-1) (Axonal glycoprotein TAG-1) (Transient axonal glycoprotein 1) (TAX-1)
73564 (4)26.04.5-41.969.5 19818/6.7HUMANSorting nexin 24 (SBBI31)
83474 (4)38.04.539.847.1 18355/4.9HUMANHypothetical protein FLJ22875
93464 (4)38.04.539.847.1 18418/4.8MOUSEHypothetical protein FLJ22875 homolog
102764 (4)22.04.5-0.18891.4 18927/11.7MOUSEFibroblast growth factor-22 precursor (FGF-22)
15201...........................x..................x.........x..x......................x......
100353.............xx........x................xxx........................xx....................
111946....................x.............................x............................x...x.....
58513...........................x..................x............x......................x......
46553.........................x.........x......x.......................................x......
63408.................x.x...................................x.......x.........................
55994.....................xx.....................................x..............x.............
11833..........................x........x................................x......x.............
91854..........................x........x................................x......x.............
50917......................x..........................x.x......x..............................



Detailed Results

1. 5/89 matches (5%).
Acc. #: Species: MOUSE Name: Proteasome subunit beta type 3 (Proteasome theta chain) (Proteasome chain 13) (Proteasome component C10-II)
Index: MW: 22965 Da pI: 6.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1234.70781234.67962371801(R) (Q)
1613.94081613.79988728410(R) (I)
1797.05211796.9580521781941(R) (I)
1889.16581889.04966249660(R) (L)
2705.37482705.4449-2642661(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (66/205AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/89 matches (8%).
Acc. #: Species: HUMAN Name: Adenylate kinase isoenzyme 5 (ATP-AMP transphosphorylase)
Index: MW: 22088 Da pI: 5.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1037.52841037.5420-13921000(R) (E)
1045.51821045.5828-6259661(K) (G)
1158.60881158.6887-6913240(K) (G)
1460.83651460.82994.511241(K) (G)
1475.82811475.7957221381511(R) (R)
1493.80261493.77531835470(K) (E)
2150.24462150.1174591531701(R) (T)
2184.25522184.0759821Met-ox 811001(K)EAMVASLGDTRGFLIDGYPR(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 43% (87/198AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/89 matches (4%).
Acc. #: Species: MOUSE Name: Glandular kallikrein K22 precursor (Tissue kallikrein) (MGK-22) (Epidermal growth factor-binding protein type A) (EGF-BP A) (Nerve growth factor beta chain endopeptidase) (Beta-NGF-endopeptidase)
Index: MW: 28384 Da pI: 6.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1109.50621109.5777-641761840(K) (A)
1671.98211671.9369271761891(K) (V)
2495.21072495.2182-3.058771(R) (N)
2872.68042872.39809834570(K) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (58/259AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/89 matches (4%).
Acc. #: Species: HUMAN Name: Proteasome subunit beta type 3 (Proteasome theta chain) (Proteasome chain 13) (Proteasome component C10-II)
Index: MW: 22949 Da pI: 6.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1234.70781234.67962371801(R) (Q)
1613.94081613.79988728410(R) (I)
1889.16581889.04966249660(R) (L)
2705.37482705.4449-2642661(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (49/205AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/89 matches (4%).
Acc. #: Species: HUMAN Name: HLA class II histocompatibility antigen, DR-7 beta chain precursor
Index: MW: 29822 Da pI: 7.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1193.63991193.5703581601681(R) (A)
1344.72031344.65884659680(R) (F)
1493.80261493.7137591101220(R) (R)
2705.37482705.2703391962180(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (55/266AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 4/89 matches (4%).
Acc. #: Species: MOUSE Name: Contactin 2 (Axonin-1) (Axonal glycoprotein TAG-1) (Transient axonal glycoprotein 1) (TAX-1)
Index: MW: 23741 Da pI: 5.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1090.54051090.491845pyroGlu 921010(R)QGSSSWQTAR(V)
1107.55101107.518330921010(R) (V)
1765.84801765.81861776900(R) (R)
1922.10431921.91979676911(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (26/218AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 4/89 matches (4%).
Acc. #: Species: HUMAN Name: Sorting nexin 24 (SBBI31)
Index: MW: 19818 Da pI: 6.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1141.52721141.5716-39190(-) (Y)
1157.56371157.5665-2.51Met-ox 190(-)MEVYIPSFR(Y)
1908.93991909.1064-87951101(K) (A)
2343.16412343.2560-3975941(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (45/169AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 4/89 matches (4%).
Acc. #: Species: HUMAN Name: Hypothetical protein FLJ22875
Index: MW: 18355 Da pI: 4.9
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1201.65861201.5999491Met-ox 1391490(R)VAAAMENPNLR(E)
1344.72031344.6357631501600(R) (-)
2184.25522184.167340831010(R) (V)
2343.16412343.14687.41121311(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 38% (61/160AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 4/89 matches (4%).
Acc. #: Species: MOUSE Name: Hypothetical protein FLJ22875 homolog
Index: MW: 18418 Da pI: 4.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1201.65861201.5999491Met-ox 1391490(R)VAAAMENPNLR(E)
1344.72031344.6357631501600(R) (-)
2184.25522184.167340831010(K) (V)
2343.16412343.14687.41121311(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 38% (61/160AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 4/89 matches (4%).
Acc. #: Species: MOUSE Name: Fibroblast growth factor-22 precursor (FGF-22)
Index: MW: 18927 Da pI: 11.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1157.56371157.6391-6546561(R) (W)
1657.90451657.8848121271410(R) (Q)
1673.95011673.8797421Met-ox 1271410(R)GRPMFLALDSQGIPR(Q)
1852.00641851.98701136511(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (36/162AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


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