MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D147
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 1841 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/101(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
19.841e+00511 (10)22.010.9-15.853.9 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
28.932e+0048 (7)22.07.93.7384.2 57933/8.7HUMANZinc finger protein clone 647
38.300e+0046 (5)14.05.90.65655.3 71289/6.0MOUSEGTP-RHO BINDING PROTEIN 1 (RHOPHILIN)
47.247e+0048 (7)25.07.9-18.254.6 58629/9.1MOUSET-box transcription factor TBX6 (T-box protein 6)
55.558e+00414 (13)21.013.9-26.468.8 89615/8.6HUMANTumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
64.955e+0046 (5)12.05.9-23.865.3 63058/5.3MOUSEGlypican-6 precursor
73.288e+00411 (10)30.010.97.1294.6 68206/8.6HUMANZinc finger protein 23 (Zinc finger protein KOX16) (DKFZp569D2231)
83.134e+0047 (6)24.06.9-22.261.8 38715/6.6HUMANAnnexin I (Lipocortin I) (Calpactin II) (Chromobindin 9) (P35) (Phospholipase A2 inhibitory protein)
92.441e+00410 (9)19.09.9-13.178.8 70573/9.4HUMANZinc finger protein 93 (Zinc finger protein HTF34)
102.354e+0047 (6)24.06.9-45.140.4 57888/7.2MOUSEPyruvate kinase, M2 isozyme
45313.............x....x...........x.xx...x.....x...x......................x...x........x.................
25321...........xx...........................x.........xx...x.....x........................x..............
102436......................................................x.x.x.................x.x.....x................
61173..............................................x......x.............x........x..x.....xx..........x...
42717.x................x...x.....x........x...........x......x.xxx..x......x..x..................x........
70838...............................................x..........x.x...............x.x.............x........
6922........................................x............xxx.x...x....x............x.xxx.................
52500.......x.............x..................x.x.x.x........................x.............................
532......x...........x...................xxx...........xx.x...........x.x...............................
67752..............................x......................x...........xx....................x..x...x......


Detailed Results

1. 11/101 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1092.49171092.5034-116036160(R) (G)
1179.57891179.6010-193773860(K) (H)
1340.63631340.6698-253653761(K) (Y)
1383.63121383.6909-431861970(K) (V)
1421.65571421.7389-592772881(K) (K)
1475.73891475.7494-7.12122230(K) (T)
1475.73891475.7858-322002110(R) (W)
1638.84341638.8604-101861991(K) (F)
1751.91921751.8272536036241(R) (S)
1993.95241993.9772-122242390(R) (R)
2150.02862150.0783-232242401(R) (R)
2383.94962383.9524-1.25195490(R) (G)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 22% (142/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/101 matches (7%).
Acc. #: Species: HUMAN Name: Zinc finger protein clone 647
Index: MW: 57933 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1086.48281086.5220-3627350(R) (K)
1090.46291090.5017-361041120(R) (Q)
1543.81011543.696474pyroGlu 1131250(K)QSFCLSPNSVDHR(E)
1794.83581794.8155113103240(R) (A)
1820.72481820.8060-454784920(R) (A)
1849.90981849.8213481982120(R) (A)
1921.94431921.93773.52262401(K) (A)
2454.33302454.30929.776980(K) (T)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 22% (114/510AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/101 matches (5%).
Acc. #: Species: MOUSE Name: GTP-RHO BINDING PROTEIN 1 (RHOPHILIN)
Index: MW: 71289 Da pI: 6.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1838.90121838.9149-7.559741(R) (E)
1851.90121851.8289394624770(R) (T)
1902.91851902.9211-1.428440(K) (A)
2211.08212211.1331-235645840(R) (G)
2227.05942227.1280-311Met-ox 5645840(R)SMGEEGVSLQVVSLLPSPEPR(G)
2418.23192418.1651281681880(R) (F)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 14% (91/643AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/101 matches (7%).
Acc. #: Species: MOUSE Name: T-box transcription factor TBX6 (T-box protein 6)
Index: MW: 58629 Da pI: 9.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1739.76341739.8142-291531671(R) (L)
1837.87881837.8808-1.11721870(R) (Q)
1939.96081939.94896.11161(-) (L)
2211.08212211.0835-0.6217370(R) (Y)
2283.17202283.2171-203733930(R) (G)
2437.14322437.2675-512202401(R) (F)
2454.33302454.3033122412611(R) (I)
2705.14862705.3159-621Met-ox 1721941(R)VYIHPDSPATGAHWMRQPVSFHR(V)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 25% (138/540AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 14/101 matches (13%).
Acc. #: Species: HUMAN Name: Tumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
Index: MW: 89615 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1010.43391010.4907-561321391(K) (F)
1179.57891179.5581186776850(K) (F)
1271.61071271.56653553610(R) (E)
1320.58131320.6548-567507611(R) (C)
1475.73891475.6841371Met-ox 2882990(K)VHFLTDPENEMK(E)
1791.74611791.8601-646776901(K) (R)
1851.90121851.9751-404214390(K) (G)
1902.91851903.0111-49AcetN 1171(-)AEQVLPQALYLSNMRK(A)
1917.89931917.9969-516446601(K) (E)
1918.95091919.0061-29AcetN 1Met-ox 1171(-)AEQVLPQALYLSNMRK(A)
1925.85761925.9326-395155301(R) (T)
1993.95241994.0500-4925410(R) (T)
2091.95282092.0061-252832991(R) (E)
2540.11192540.1186-2.62Met-ox 1631831(R)NWNDEWDNLIKMASTDTPMAR(S)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 21% (167/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 6/101 matches (5%).
Acc. #: Species: MOUSE Name: Glypican-6 precursor
Index: MW: 63058 Da pI: 5.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1751.91921751.8791233984111(K) (V)
1902.91851902.9523-183023180(R) (I)
1918.95091918.94721.91Met-ox 3023180(R)LEGPFNIESVMDPIDVK(I)
2211.08212211.1670-385345520(K) (L)
2227.05942227.1619-461Met-ox 5345520(K)FSSSLISWSLVCMVLALQR(L)
2540.11192540.2785-66811011(K) (H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 12% (71/555AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 11/101 matches (10%).
Acc. #: Species: HUMAN Name: Zinc finger protein 23 (Zinc finger protein KOX16) (DKFZp569D2231)
Index: MW: 68206 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1543.81011543.7361481Met-ox 2072201(K)MCGKAFSVNGSLSR(H)
1837.87881837.86557.2pyroGlu 3043180(R)QHQSIHTGEKPYQCK(E)
1838.90121839.0129-611061201(K) (C)
1849.90981849.8213483924060(R) (A)
1849.90981849.8213483643780(R) (A)
1849.90981849.8213482802940(R) (G)
1874.91871874.8595321Met-ox 9231(K)EMYEGKENVSFELQR(D)
1921.94431921.8974244764901(R) (A)
1932.94711932.9204145785941(R) (-)
2283.17202283.0511534174341(R) (C)
2342.96512343.0974-563043221(R) (G)
2369.03542369.1270-393363551(R) (G)
2383.94962384.1240-733884061(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 30% (182/594AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 7/101 matches (6%).
Acc. #: Species: HUMAN Name: Annexin I (Lipocortin I) (Calpactin II) (Chromobindin 9) (P35) (Phospholipase A2 inhibitory protein)
Index: MW: 38715 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1055.45821055.5269-65190(-) (Q)
1262.58651262.6017-121141240(K) (A)
1543.81011543.8695-3959721(K) (N)
1605.87071605.9580-54991130(K) (T)
1702.87261702.8863-8.11291440(K) (T)
1739.76341739.7360161892040(R) (A)
2067.89962067.88556.81862041(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (84/346AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 10/101 matches (9%).
Acc. #: Species: HUMAN Name: Zinc finger protein 93 (Zinc finger protein HTF34)
Index: MW: 70573 Da pI: 9.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1052.52481052.5740-473453540(K) (H)
1179.57891179.6526-623733820(K) (H)
1488.69621488.7633-453303411(R) (C)
1489.74771489.74730.232182291(R) (C)
1543.81011543.8167-4.35535641(K) (E)
1834.93481834.8468483023160(K) (A)
1837.87881837.8213314144281(R) (A)
1849.90981849.8577283864001(R) (A)
1939.96081940.0605-512332501(K) (K)
1962.88481962.9417-293013161(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 19% (120/616AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 7/101 matches (6%).
Acc. #: Species: MOUSE Name: Pyruvate kinase, M2 isozyme
Index: MW: 57888 Da pI: 7.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1340.63631340.7300-704374471(R) (A)
1837.87881837.9118-181Met-ox 2792941(R)RFDEILEASDGIMVAR(G)
1931.93341931.9867-283843990(R) (R)
1932.94711933.0325-443203360(R) (K)
2493.19922493.2877-35931150(R) (G)
2514.08802514.2662-714764981(K) (A)
2572.14692572.2757-502252461(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (128/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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