MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D149
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 1753 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/100(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
16.303e+00612 (12)33.012.0-0.51437.3 29717/6.6HUMANLIM and SH3 domain protein 1 (LASP-1) (MLN 50)
26.245e+00612 (12)27.012.0-4.0328.6 29995/6.6MOUSELIM AND SH3 DOMAIN PROTEIN 1 (LASP-1) (MLN 50)
31.891e+00610 (10)27.010.0-11.248.6 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
41.261e+00513 (13)48.013.01.4536.5 36054/8.6HUMANGlyceraldehyde 3-phosphate dehydrogenase, liver
51.195e+0059 (9)18.09.0-20.346.6 78011/8.7HUMANCytoplasmic tyrosine-protein kinase BMX (Bone marrow kinase BMX) (Epithelial and endothelial tyrosine kinase) (ETK) (NTK38)
69.479e+00410 (10)12.010.0-3.8357.7 91800/5.5MOUSEHYALURONAN MEDIATED MOTILITY RECEPTOR (INTRACELLULAR HYALURONIC ACID BINDING PROTEIN) (RECEPTOR FOR HYALURONAN-MEDIATED MOTILITY)
77.223e+0049 (9)15.09.00.92949.3 85459/9.0HUMANZinc finger protein 84 (Zinc finger protein HPF2)
84.569e+0048 (8)12.08.0-11.169.0 83310/6.2HUMANLanosterol synthase (Oxidosqualene--lanosterol cyclase) (2,3-epoxysqualene--lanosterol cyclase) (OSC)
94.097e+0046 (6)23.06.07.4939.6 45570/9.3HUMANAlpha-(1,3)-fucosyltransferase (Galactoside 3-L-fucosyltransferase) (Fucosyltransferase 4) (FUCT-IV) (ELAM-1 ligand fucosyltransferase)
104.083e+00410 (10)19.010.05.8462.2 69501/8.2HUMANBifunctional 3'-phosphoadenosine 5'-phosphosulfate synthethase 2 (PAPS synthethase 2) (PAPSS 2) (Sulfurylase kinase 2) (SK2) (SK 2) [Includes: Sulfate adenylyltransferase (Sulfate adenylate transferase) (SAT) (ATP-sulfurylase); Adenylylsulfate kinase (>
57762...xx........xx................x..x............xxxx...x...x.........................................
64687...xx........xx................x..x............xxxx...x...x.........................................
45313.........x.x.......x.....x..x......x.......................x.........x.............x............x...
108737...............x..............x..........x.........x..............x...........x......x.....xxx.x.xx.
33767.............x..........x......x..x................x............x...x.................x......x......
94157..........x.............x...x..................x.x.x....x....x.......x...x..........................
1578.......x...................x............xx.......................x..xx..x..x........................
17712....xxx...................x...........x..x........x.....................................x...........
105094.............................................x...........x.......x.............x.....x.x............
80772x..........x........x....x...............x......x....x......x...................x.......x...........


Detailed Results

1. 12/100 matches (12%).
Acc. #: Species: HUMAN Name: LIM and SH3 domain protein 1 (LASP-1) (MLN 50)
Index: MW: 29717 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
972.4667972.4678-1.286921(R) (N)
981.4118981.4318-201221280(K) (S)
1185.59971185.586411pyroGlu 76850(K)QQSELQSQVR(Y)
1202.60731202.6129-4.776850(K) (Y)
1418.71411418.7280-9.8971090(K) (I)
1443.78341443.7920-5.974851(R) (Y)
1591.76481591.742614pyroGlu 60730(K)QSFTMVADTPENLR(L)
1603.84361603.8444-0.48951091(K) (I)
1607.75901607.737513pyroGlu 1Met-ox 60730(K)QSFTMVADTPENLR(L)
1608.74161608.6746422Met-ox 1291431(K)SRMGPSGGEGMEPER(R)
1608.74161608.7692-1760730(K) (L)
1624.77251624.76415.21Met-ox 60730(K)QSFTMVADTPENLR(L)
1678.83331678.8877-321882051(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 33% (88/261AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/100 matches (12%).
Acc. #: Species: MOUSE Name: LIM AND SH3 DOMAIN PROTEIN 1 (LASP-1) (MLN 50)
Index: MW: 29995 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
972.4667972.4678-1.286921(R) (N)
981.4118981.4318-201221280(K) (S)
1185.59971185.586411pyroGlu 76850(K)QQSELQSQVR(Y)
1202.60731202.6129-4.776850(K) (Y)
1418.71411418.7280-9.8971090(K) (I)
1443.78341443.7920-5.974851(R) (Y)
1591.76481591.742614pyroGlu 60730(K)QSFTMVADTPENLR(L)
1603.84361603.8444-0.48951091(K) (I)
1607.75901607.737513pyroGlu 1Met-ox 60730(K)QSFTMVADTPENLR(L)
1608.74161608.7692-1760730(K) (L)
1624.77251624.76415.21Met-ox 60730(K)QSFTMVADTPENLR(L)
1678.83331678.8877-321902071(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (73/263AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/100 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1141.52281141.51992.54644720(R) (L)
1179.59661179.6010-3.73773860(K) (H)
1302.67111302.7018-243934031(R) (V)
1302.67111302.7157-343443550(R) (A)
1383.66191383.6909-211861970(K) (V)
1393.66431393.7327-492782891(R) (D)
1475.74741475.7494-1.42122230(K) (T)
1475.74741475.7858-262002110(R) (W)
1687.90841687.9707-3766821(R) (G)
1779.80721779.75093246650(R) (S)
1994.00891993.9772162242390(R) (R)
2383.97872383.9524115195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (174/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 13/100 matches (13%).
Acc. #: Species: HUMAN Name: Glyceraldehyde 3-phosphate dehydrogenase, liver
Index: MW: 36054 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1233.61461233.5972142Met-ox 3243340(R)VVDLMAHMASK(E)
1411.74761411.7909-312012150(R) (A)
1544.81001544.8107-0.412352480(R) (L)
1613.90151613.9015-0.03867800(K) (D)
1763.80421763.80290.723103230(K) (V)
1861.93951861.9516-6.51461620(K) (V)
2041.11442041.10823.067841(K) (I)
2211.13092211.2171-392352541(R) (Y)
2229.12292229.10488.11Met-ox 1191390(R)VIISAPSADAPMFVMGVNHEK(Y)
2245.13602245.0997162Met-ox 1191390(R)VIISAPSADAPMFVMGVNHEK(Y)
2293.07232293.0335171Met-ox 871070(K)WGDAGAEYVVESTGVFTTMEK(A)
2401.25382401.2008222Met-ox 1181391(K)RVIISAPSADAPMFVMGVNHEK(Y)
2611.39262611.3554141Met-ox 1631860(K)VIHDNFGIVEGLMTTVHAITATQK(T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 48% (162/335AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 9/100 matches (9%).
Acc. #: Species: HUMAN Name: Cytoplasmic tyrosine-protein kinase BMX (Bone marrow kinase BMX) (Epithelial and endothelial tyrosine kinase) (ETK) (NTK38)
Index: MW: 78011 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1185.59971185.59761.72652741(K) (I)
1185.59971185.6308-2677850(R) (D)
1377.61531377.6228-5.42382470(R) (K)
1418.71411418.7644-354224350(K) (W)
1443.78341443.8310-33AcetN 1131(-)DTKSILEELLLK(R)
1613.90151613.8539293994130(K) (R)
1746.77661746.8413-373593720(K) (L)
1777.83151777.8920-343733870(K) (L)
2042.10942042.1398-156566721(K) (D)
2245.13602245.2444-484154351(R) (W)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (124/675AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 10/100 matches (10%).
Acc. #: Species: MOUSE Name: HYALURONAN MEDIATED MOTILITY RECEPTOR (INTRACELLULAR HYALURONIC ACID BINDING PROTEIN) (RECEPTOR FOR HYALURONAN-MEDIATED MOTILITY)
Index: MW: 91800 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1165.57721165.5411312252331(K) (L)
1377.61531377.6684-391051151(K) (T)
1393.66431393.66330.721Met-ox 1051151(K)RIQDMESELEK(T)
1591.76481591.8001-223113240(R) (L)
1607.75901607.7951-221Met-ox 3113240(R)AETLSAEMQILTER(L)
1613.90151613.9127-7.04304440(K) (Y)
1650.85171650.7896381061191(R) (L)
1707.77761707.7471187817941(K) (-)
1779.80721779.8917-486746880(K) (Q)
1795.81801795.79611211270(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (99/794AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 9/100 matches (9%).
Acc. #: Species: HUMAN Name: Zinc finger protein 84 (Zinc finger protein HPF2)
Index: MW: 85459 Da pI: 9.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1100.57791100.5853-6.77047120(R) (C)
1391.68191391.6378325645740(R) (D)
1542.84571542.8141216917031(K) (I)
1544.81001544.7934115795911(K) (I)
1761.83921761.9176-4421341(K) (D)
1777.83151777.8365-2.85926050(R) (K)
1779.80721779.8270-113683821(R) (A)
1792.80321792.8362-185085220(R) (A)
1808.82191808.7696296206330(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (118/738AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 8/100 matches (8%).
Acc. #: Species: HUMAN Name: Lanosterol synthase (Oxidosqualene--lanosterol cyclase) (2,3-epoxysqualene--lanosterol cyclase) (OSC)
Index: MW: 83310 Da pI: 6.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
981.4118981.4498-39180(-) (R)
995.4522995.4984-466156220(R) (Q)
1094.52961094.52316.06646720(R) (G)
1388.68601388.7394-384834940(R) (N)
1500.79351500.7334404204320(R) (Y)
1544.81001544.7756224714821(K) (L)
1608.74161608.8121-4447610(R) (N)
2083.03562083.0136112612770(R) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (94/732AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 6/100 matches (6%).
Acc. #: Species: HUMAN Name: Alpha-(1,3)-fucosyltransferase (Galactoside 3-L-fucosyltransferase) (Fucosyltransferase 4) (FUCT-IV) (ELAM-1 ligand fucosyltransferase)
Index: MW: 45570 Da pI: 9.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1584.82331584.81346.22122270(R) (K)
1671.90141671.8495311831960(R) (A)
1761.83921761.8488-5.51972110(R) (S)
1929.92621929.90709.91671820(R) (S)
2041.11442041.0619261021191(R) (G)
2067.89222067.9387-233663810(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (95/405AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 10/100 matches (10%).
Acc. #: Species: HUMAN Name: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthethase 2 (PAPS synthethase 2) (PAPSS 2) (Sulfurylase kinase 2) (SK2) (SK 2) [Includes: Sulfate adenylyltransferase (Sulfate adenylate transferase) (SAT) (ATP-sulfurylase); Adenylylsulfate kinase (>
Index: MW: 69501 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
864.4672864.4943-315525591(R) (K)
1179.59661179.5468425135230(R) (K)
1307.66651307.6418195135241(R) (D)
1383.66191383.65137.73423521(R) (H)
1544.81001544.80086.03103231(R) (R)
1603.84361603.782938881011(R) (R)
1618.77801618.8342-353253371(R) (K)
1702.88771702.8447254124260(R) (R)
1940.94291940.8878281741900(K) (V)
2083.03562083.1228-422522690(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (122/614AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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