MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D151
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 2674 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/100(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.852e+00914 (14)21.014.0-24.274.9 83314/5.6HUMANPolymeric-immunoglobulin receptor precursor (Poly-IG receptor) (PIGR) [Contains: Secretory component]
21.388e+00612 (12)21.012.0-31.253.8 96866/5.3HUMANHeat-shock protein 105 kDa (Heat shock 110 kDa protein) (Antigen NY-CO-25)
39.821e+00513 (13)28.013.0-28.688.6 97018/5.4HUMANProtein 4.1 (Band 4.1) (P4.1) (EPB4.1) (4.1R)
49.542e+00512 (12)23.012.0-12.485.8 89615/8.6HUMANTumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
56.110e+00510 (10)23.010.0-14.994.6 67375/5.5HUMANLutheran blood group glycoprotein precursor (B-CAM cell surface glycoprotein) (Auberger B antigen) (F8/G253 antigen)
66.031e+0058 (8)16.08.0-12.649.8 78339/8.6HUMANLactotransferrin precursor (Lactoferrin) [Contains: Lactoferroxin A; Lactoferroxin B; Lactoferroxin C]
75.295e+00510 (10)20.010.0-34.764.4 96493/5.4MOUSEHeat-shock protein 105 kDa (Heat shock-related 100 kDa protein E7I) (HSP-E7I) (Heat shock 110 kDa protein) (42 degrees C-HSP)
84.152e+0056 (6)55.06.0-14.368.8 15759/8.7MOUSEIg heavy chain V region MOPC 141 precursor
93.580e+00513 (13)19.013.0-37.682.5 93838/7.8HUMANGlycerol-3-phosphate acyltransferase, mitochondrial precursor (GPAT)
102.636e+00512 (12)25.012.0-18.579.4 76931/7.0HUMANGlucosamine--fructose-6-phosphate aminotransferase [isomerizing] 2 (Hexosephosphate aminotransferase 2) (D-fructose-6-phosphate amidotransferase 2) (GFAT 2) (GFAT2)
51794.......x.x.......x......................x........x........x.x.....x...xx..x...x..x........x.........
27666.x..x......x....x................x.......xx.................x.............xx...........x...x........
80664..........x...x...............x.....x........x...x..............x.......xx...x.....x.....x..x.......
42717............x...x..........x...x...x..........x.....x....x.......x...x.x............x...............
4123..x.........x.........x...x.....x.............x.................x.............x.....x.....x.........
89781...x........x............x.................x.................x.x............................x......x
99000....x......x....x.............x..........xx.................x.............xx...........x............
55962...................................................x......x......................x...x.......x....x.
95809............................x......x...x..x...x.........x......x..x...x...................x..xxx....
111219...............x.x........x....xx..x.x..x............................x..............x............xx.


Detailed Results

1. 14/100 matches (14%).
Acc. #: Species: HUMAN Name: Polymeric-immunoglobulin receptor precursor (Poly-IG receptor) (PIGR) [Contains: Secretory component]
Index: MW: 83314 Da pI: 5.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1250.62281250.6745-413053160(K) (K)
1378.64891378.7694-873053171(K) (E)
1539.67851539.6523176236380(R) (A)
1813.85951813.8406104804931(K) (Y)
1881.97381881.9070353803941(K) (C)
2008.91902008.9405-11pyroGlu 5485650(K)QGHFYGETAAVYVAVEER(K)
2025.93152025.9670-185485650(K) (K)
2114.11492114.1974-391932110(R) (L)
2151.05172151.2326-841190(-) (S)
2154.08072154.06208.75485661(K) (A)
2172.07672172.2028-582973161(R) (K)
2214.00622213.99853.56917120(R) (E)
2241.07662241.1767-452322500(K) (G)
2383.30992383.3825-301912111(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 21% (166/764AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/100 matches (12%).
Acc. #: Species: HUMAN Name: Heat-shock protein 105 kDa (Heat shock 110 kDa protein) (Antigen NY-CO-25)
Index: MW: 96866 Da pI: 5.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
907.3610907.3831-248518580(K) (-)
1196.59701196.5985-1.234440(R) (N)
1445.69551445.8229-884594711(K) (D)
1522.76031522.7728-8.22402511(K) (Y)
1765.76751765.8696-581Met-ox 7157281(K)MFEELGQRLQHYAK(I)
1818.88291818.9723-493753901(R) (E)
1834.87811834.9373-324845000(R) (V)
2025.93152025.9738-211Met-ox 3013161(K)MNRSQFEELCAELLQK(I)
2025.93152026.0102-391190(-) (A)
2172.07672172.0391176096261(K) (L)
2187.98142188.0340-241Met-ox 6096261(K)DLLNMYIETEGKMIMQDK(L)
2343.00172343.0671-282762950(K) (D)
2526.13632526.2637-501972191(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (187/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 13/100 matches (13%).
Acc. #: Species: HUMAN Name: Protein 4.1 (Band 4.1) (P4.1) (EPB4.1) (4.1R)
Index: MW: 97018 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1400.67181400.7095-271Met-ox 3623721(K)ELEEKVMELHK(S)
1470.76301470.8280-441932051(K) (K)
1707.79271707.8931-595195330(R) (T)
1793.85081793.9649-647487631(K) (T)
1847.89581848.0231-697167330(R) (T)
1881.97381881.9347214514660(K) (L)
1881.97381882.0009-143033171(R) (L)
2104.04952103.9922276917071(K) (L)
2162.97902163.1661-878208390(R) (E)
2171.01982171.0443-113733911(K) (K)
2211.02222211.1257-476166351(K) (L)
2302.23792302.1654314794971(K) (S)
2363.07012363.2896-934034241(K) (D)
2537.27142537.1830351Met-ox 8438641(K)EQHPDMSVTKVVVHQETEIADE(-)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 28% (243/864AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 12/100 matches (12%).
Acc. #: Species: HUMAN Name: Tumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
Index: MW: 89615 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1459.72721459.6891262882990(K) (E)
1522.76031522.8342-482652781(K) (D)
1665.74641665.8634-701Met-ox 2142281(K)MLRSLESGSNFAPLK(V)
1742.81601742.8284-7.17437591(R) (Q)
1791.94121791.8601456776901(K) (R)
1851.91091851.9751-354214390(K) (G)
1918.88521919.0061-63AcetN 1Met-ox 1171(-)AEQVLPQALYLSNMRK(A)
1993.95341994.0500-4825410(R) (T)
2107.93632108.0010-311Met-ox 2832991(R)FEDLKVHFLTDPENEMK(E)
2148.09582147.9755564804970(R) (C)
2154.08072154.0395193383540(K) (K)
2307.12802307.11137.23663860(R) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (186/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/100 matches (10%).
Acc. #: Species: HUMAN Name: Lutheran blood group glycoprotein precursor (B-CAM cell surface glycoprotein) (Auberger B antigen) (F8/G253 antigen)
Index: MW: 67375 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
924.3959924.4613-711221280(R) (A)
1459.72721459.71816.24524641(K) (E)
1579.73621579.7651-181Met-ox 1151(-)MEPPDAPAQARGAPR(L)
1623.76581623.8958-802112250(R) (L)
1743.78901743.9281-801411560(R) (G)
1851.91091851.8621261922070(R) (T)
2104.04952103.98033378960(R) (G)
2214.00622214.0573-235115311(K) (R)
2307.12802307.0862181882071(R) (T)
2383.30992383.2159394774991(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (149/628AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 8/100 matches (8%).
Acc. #: Species: HUMAN Name: Lactotransferrin precursor (Lactoferrin) [Contains: Lactoferroxin A; Lactoferroxin B; Lactoferroxin C]
Index: MW: 78339 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1097.48731097.5056-175455530(R) (C)
1459.72721459.7368-6.63653760(R) (K)
1614.80601614.8604-342792921(R) (Q)
1835.90611835.8268435215360(R) (C)
2071.02572071.0388-6.374930(R) (L)
2088.03462088.0766-203343520(R) (K)
2537.27142537.3516-323303521(R) (K)
2872.30632872.3899-294374620(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (120/711AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 10/100 matches (10%).
Acc. #: Species: MOUSE Name: Heat-shock protein 105 kDa (Heat shock-related 100 kDa protein E7I) (HSP-E7I) (Heat shock 110 kDa protein) (42 degrees C-HSP)
Index: MW: 96493 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1196.59701196.5985-1.234440(R) (N)
1445.69551445.8229-884594711(K) (D)
1522.76031522.7728-8.22402511(K) (Y)
1707.79271707.9468-903173310(K) (A)
1818.88291818.9723-493753901(R) (E)
1834.87811834.9373-324845000(R) (V)
2025.93152025.9738-211Met-ox 3013161(K)MNRSQFEELCAELLQK(I)
2025.93152026.0102-391190(-) (A)
2172.07672172.0391176106271(R) (L)
2187.98142188.0340-241Met-ox 6106271(R)DLLNMYIETEGKMIMQDK(L)
2343.00172343.0671-282762950(K) (D)
2343.00172343.1266-531972170(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (178/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 6/100 matches (6%).
Acc. #: Species: MOUSE Name: Ig heavy chain V region MOPC 141 precursor
Index: MW: 15759 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1910.80181910.8812-421281440(K) (-)
2008.91902008.91153.71121271(R) (Y)
2241.07662241.0352181251441(R) (-)
2312.18132312.11992763830(K) (S)
2555.12832555.2530-4963851(K) (L)
2751.37932751.5015-441240(-) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 55% (80/144AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 13/100 matches (13%).
Acc. #: Species: HUMAN Name: Glycerol-3-phosphate acyltransferase, mitochondrial precursor (GPAT)
Index: MW: 93838 Da pI: 7.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1682.76971682.8866-693593730(R) (K)
1791.94121792.0121-402162310(K) (S)
1810.88691810.9816-523593741(R) (N)
1834.87811834.9452-37pyroGlu 3063201(R)QQQFLEIFLEGTRSR(S)
1851.91091851.9717-333063201(R) (S)
1975.83791976.0354-1007087231(K) (L)
2088.03462088.0436-4.37637801(R) (N)
2114.11492114.09569.27667841(R) (M)
2151.05172151.0908-181Met-ox 4965131(R)HRQGIDLSTLVEDFFVMK(E)
2383.30992383.1741571391610(R) (A)
2555.12832555.3067-701Met-ox 4985191(R)QGIDLSTLVEDFFVMKEEVLAR(D)
2569.29822569.4614-641731951(R) (L)
2593.24062593.4176-681Met-ox 2092311(K)GQLEMVKAATETNLPLLFLPVHR(S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (162/828AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 12/100 matches (12%).
Acc. #: Species: HUMAN Name: Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 2 (Hexosephosphate aminotransferase 2) (D-fructose-6-phosphate amidotransferase 2) (GFAT 2) (GFAT2)
Index: MW: 76931 Da pI: 7.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1504.72681504.8375-743453580(R) (D)
1539.67851539.7542-491611730(R) (V)
1623.76581623.9110-892122241(K) (T)
1742.81601742.80605.71Met-ox 3293420(K)EIFEQPESVFNTMR(G)
1743.78901743.8679-45931080(R) (S)
1791.94121791.8497511141(-) (T)
1797.86981797.85816.565791(K) (V)
1813.85951813.85303.61Met-ox 65791(K)ALDEELYKQDSMDLK(V)
2148.09582148.1089-6.11091271(R) (D)
2307.12802307.10061230501(R) (E)
2737.23172737.2754-163203421(K) (G)
2751.37932751.4391-221Met-ox 5625861(K)EITYMHSEGILAGELKHGPLALIDK(Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (173/682AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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