MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D153
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1304 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/56(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.691e+0047 (12)18.012.535.532.2 40308/6.3HUMANSialic acid synthase (N-acetylneuraminate synthase) (N-acetylneuraminic acid synthase) (N-acetylneuraminate-9-phosphate synthase) (N-acetylneuraminic acid phosphate synthase)
2490710 (17)11.017.919.2121 92868/5.3MOUSEDNA REPLICATION LICENSING FACTOR MCM6 (MIS5 HOMOLOG)
3334411 (19)16.019.69.7995.6 68867/8.3HUMANF-box/WD-repeat protein 1B (F-box and WD-repeats protein beta-TrCP) (E3RSIkappaB) (pIkappaBalpha-E3 receptor subunit)
4329510 (17)17.017.916.276.4 80897/9.1MOUSEPlakophilin 1
524879 (16)14.016.1-24.8102 67611/6.5HUMANVacuolar protein sorting 33A (hVPS33A)
6245110 (17)13.017.91.6977.7 80625/7.3HUMANProtein-glutamine gamma-glutamyltransferase 3-like (Transglutaminase 3-like) (TGase 3-like)
722069 (16)13.016.1-7.75106 65866/8.1HUMANKeratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
821918 (14)11.014.3-13.091.0 91612/6.7HUMANSerine/threonine-protein kinase ANKRD3 (Ankyrin repeat domain protein 3) (PKC-delta-interacting protein kinase)
917936 (10)25.010.7-15.794.5 27978/5.9MOUSEDEAD-box RNA helicase DEAD2 (mDEAD2) (D1PAS1 related sequence 1)
1016868 (14)10.014.30.349129 78580/6.0HUMANAcetyl-coenzyme A synthetase, cytoplasmic (Acetate--CoA ligase) (Acyl-activating enzyme) (Acetyl-CoA synthetase) (ACS) (AceCS)
75499..........................x.............x..x.xxx...x....
109004.......xx........xx...........x..x.....xx.x........x....
95108..x.......xx..x.........x.x....x.x..x.x............x....
108938..................x..x.........x.x.....x...xx.x....x...x
74530..x...x...x.x....x.x................x..............x...x
7360..x........x..x......xx...x......x.....x.......x.x......
114359....x......x....xx..x......x......x...x.....x...........
89318.x..........................x.....x.....x.x....x.x.x....
18751..................x.............x..x..x...........x..x..
23852..x...xx..x...................x...............x.x....x..


Detailed Results

1. 7/56 matches (12%).
Acc. #: Species: HUMAN Name: Sialic acid synthase (N-acetylneuraminate synthase) (N-acetylneuraminic acid synthase) (N-acetylneuraminate-9-phosphate synthase) (N-acetylneuraminic acid phosphate synthase)
Index: MW: 40308 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1064.58721064.57401266740(K) (H)
1331.65541331.61333287960(R) (E)
1448.77871448.7426253213330(K) (K)
1487.80811487.71446386961(K) (E)
1540.77571540.7284311321450(K) (T)
1576.90161576.8375413213341(K) (V)
1867.00381866.9197452472640(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 18% (66/359AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/56 matches (17%).
Acc. #: Species: MOUSE Name: DNA REPLICATION LICENSING FACTOR MCM6 (MIS5 HOMOLOG)
Index: MW: 92868 Da pI: 5.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
984.4307984.4977-6886920(R) (A)
986.3978986.4696-734084150(K) (A)
1026.50391026.5373-331982051(K) (V)
1029.48071029.4828-2.17157220(R) (I)
1151.72491151.61011007467540(R) (E)
1179.61961179.5758376056151(R) (I)
1307.76031307.7112387457541(K) (E)
1331.65541331.5546766346430(R) (H)
1353.69451353.6286492702820(R) (G)
1867.00381866.8755691Met-ox 3173321(R)DEEQTAESIKNQMTVK(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 11% (91/821AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/56 matches (19%).
Acc. #: Species: HUMAN Name: F-box/WD-repeat protein 1B (F-box and WD-repeats protein beta-TrCP) (E3RSIkappaB) (pIkappaBalpha-E3 receptor subunit)
Index: MW: 68867 Da pI: 8.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
966.4724966.4831-115115171(R) (R)
994.4598994.5144-554684750(R) (D)
996.5009996.420980pyroGlu 78850(R)QTYNSCAR(L)
1013.48521013.44753778850(R) (L)
1054.57361054.5421301261341(K) (E)
1064.58721064.5410431021101(K) (L)
1154.56181154.6111-432462541(R) (N)
1179.61961179.5945211Met-ox 2322401(R)MVRTDSLWR(G)
1234.69541234.6643253553660(R) (V)
1300.62211300.7126-705505601(R) (L)
1867.00381866.912849861010(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (98/605AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/56 matches (17%).
Acc. #: Species: MOUSE Name: Plakophilin 1
Index: MW: 80897 Da pI: 9.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1029.48071029.4424374894970(K) (G)
1045.56701045.56422.73193271(R) (R)
1154.56181154.6244-545315401(R) (K)
1179.61961179.60849.51Met-ox 6876960(R)LLLSDMWASK(E)
1307.76031307.6782632822920(K) (L)
1448.77871448.7605135605730(K) (E)
1475.82231475.735059pyroGlu 1Met-ox 39501(R)QRVQEQVMMTVK(R)
1540.77571540.8223-303663790(K) (V)
1867.00381866.9060523974120(R) (N)
2705.27232705.245210pyroGlu 1Met-ox 6586801(K)QYFSNSMLNNVFNLCRNTASSPK(A)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 17% (128/728AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 9/56 matches (16%).
Acc. #: Species: HUMAN Name: Vacuolar protein sorting 33A (hVPS33A)
Index: MW: 67611 Da pI: 6.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
966.4724966.5195-4963701(K) (L)
982.5371982.5144231Met-ox 63701(K)MFTLKGNR(L)
994.4598994.4814-222Met-ox 2012080(R)QVANMMIR(M)
1005.43141005.4940-62190(-) (V)
1026.50391026.5988-922632711(K) (K)
1035.45551035.5475-893173250(K) (H)
1234.69541234.6796133153251(K) (H)
1867.00381866.9134485815960(K) (-)
2705.27232705.25446.61381610(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (85/596AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 10/56 matches (17%).
Acc. #: Species: HUMAN Name: Protein-glutamine gamma-glutamyltransferase 3-like (Transglutaminase 3-like) (TGase 3-like)
Index: MW: 80625 Da pI: 7.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
966.4724966.5148-444484550(R) (Y)
996.5009996.5114-114244311(R) (K)
1013.48521013.5380-529180(K) (V)
1045.56701045.5464203823910(R) (E)
1047.50221047.5587-54pyroGlu 4654731(R)QVYSKAVNR(L)
1064.58721064.58531.84654731(R) (L)
1179.61961179.60101685940(K) (G)
1307.76031307.7099396146240(K) (V)
1307.76031307.7323215425530(R) (K)
1576.90161576.8806131Met-ox 5956081(K)ILLAAMCLVTKGEK(L)
1638.94641638.8339694414551(K) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (99/719AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 9/56 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
Index: MW: 65866 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
973.4688973.5318-654004070(K) (V)
996.5009996.5227-224374451(R) (N)
1019.47481019.4832-8.24894960(K) (M)
1026.50391026.5584-533913991(R) (I)
1041.47921041.453125180(-) (S)
1041.47921041.5580-761731810(K) (A)
1068.47031068.5036-312943020(K) (V)
1197.66501197.5500965876010(K) (H)
1300.62211300.5812311Met-ox 1101(-)MSCQISCKSR(G)
1475.82231475.7858252042150(R) (W)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (89/645AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 8/56 matches (14%).
Acc. #: Species: HUMAN Name: Serine/threonine-protein kinase ANKRD3 (Ankyrin repeat domain protein 3) (PKC-delta-interacting protein kinase)
Index: MW: 91612 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
962.3938962.4869-9764710(R) (K)
1090.51791090.5818-5964721(R) (M)
1197.66501197.6050505195290(R) (R)
1331.65541331.7006-342392490(K) (A)
1353.69451353.7061-8.65195301(R) (V)
1353.69451353.7061-8.65185291(R) (R)
1353.69451353.7279-2528401(K) (H)
1576.90161576.8620254584721(K) (I)
1638.94641638.8716465045170(K) (R)
1867.00381867.0401-196366531(K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (93/832AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 6/56 matches (10%).
Acc. #: Species: MOUSE Name: DEAD-box RNA helicase DEAD2 (mDEAD2) (D1PAS1 related sequence 1)
Index: MW: 27978 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1029.48071029.5118-301982050(R) (S)
1157.63411157.58764081900(R) (G)
1209.66141209.7683-882092200(R) (G)
1300.62211300.6398-141962051(K) (S)
1707.83951707.8964-3329431(R) (L)
1941.04021940.97923153681(K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (63/245AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 8/56 matches (14%).
Acc. #: Species: HUMAN Name: Acetyl-coenzyme A synthetase, cytoplasmic (Acetate--CoA ligase) (Acyl-activating enzyme) (Acetyl-CoA synthetase) (ACS) (AceCS)
Index: MW: 78580 Da pI: 6.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
966.4724966.5123-4191970(K) (G)
982.5371982.5072301Met-ox 91970(K)IFIEWMK(G)
984.4307984.5089-795205270(K) (T)
994.4598994.5434-8438460(R) (Y)
1151.72491151.62878489971(K) (G)
1151.72491151.6465683944021(R) (V)
1540.77571540.8561-521Met-ox 4064181(K)FYTAPTAIRLLMK(F)
1617.83491617.7794342562710(R) (R)
1941.04021940.9554445205351(K) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 10% (72/701AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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