MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D155
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1771 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/80(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
15.431e+00713 (16)43.016.3-35.651.5 37526/6.7HUMANPoly(rC)-binding protein 1 (Alpha-CP1) (hnRNP-E1) (Nucleic acid binding protein SUB2.3)
22.452e+00511 (13)16.013.8-17.573.9 80509/5.5HUMANAdseverin (Scinderin)
36.695e+00410 (12)22.012.5-6.3551.8 77888/8.7HUMANZinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
45.973e+0048 (10)12.010.0-17.869.5 74192/6.3HUMANZinc finger protein 274 (Zinc finger protein SP2114) (Zinc finger protein HFB101) (Zinc finger protein zfp2) (Zf2)
52.818e+00414 (17)15.017.5-24.163.3 95315/6.4MOUSEElongation factor 2 (EF-2)
62.208e+0048 (10)12.010.0-13.683.8 78357/8.8MOUSEDystrobrevin beta (Beta-dystrobrevin) (DTN-B) (MDTN-B)
71.620e+0047 (8)11.08.8-17.785.5 80908/5.5HUMANTRABID protein
81.149e+0047 (8)11.08.80.54473.1 85515/5.5HUMANNeprilysin (Neutral endopeptidase) (NEP) (Enkephalinase) (Common acute lymphocytic leukemia antigen) (CALLA) (Neutral endopeptidase 24.11) (CD10)
91.099e+0049 (11)16.011.3-9.1473.3 34227/6.5HUMANMitochondrial 60s ribosomal protein L39 (L39mt) (MRP-L39) (MRP-L5) (PRED22 protein)
101.023e+0049 (11)10.011.3-26.255.2 96866/5.3HUMANHeat-shock protein 105 kDa (Heat shock 110 kDa protein) (Antigen NY-CO-25)
108508x...x...........xx...................x.......x...x.....................xx.xx..xx
11094....x..x......................x.........x.......x..x..x............x......xx.x..
47898x.x.......x......x.................x.............................x.x......x.xx..
39517.............x..x.........x.........................x............x.x..........xx
39136.....x........xxxx..x.................xx...x...x..x....xx.x.....................
111702.............x..x...........................x......x......x.x.........x......x..
43647..x..........................x....x...............................x.....x.....xx
101313x..x............................x...............xx................x...x.........
103587.....xx..x...x......x.x.........................................xx..x...........
28533...................x...........x...x.........x..x.x.............xx..x...........


Detailed Results

1. 13/80 matches (16%).
Acc. #: Species: HUMAN Name: Poly(rC)-binding protein 1 (Alpha-CP1) (hnRNP-E1) (Nucleic acid binding protein SUB2.3)
Index: MW: 37526 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
997.4877997.5067-19pyroGlu 2983060(R)QGANINEIR(Q)
1014.49981014.5332-332983060(R) (Q)
1069.51621069.4658473473561(R) (-)
1086.51911086.5544-323153250(K) (Q)
1302.56321302.6112-3747570(R) (I)
1388.75161388.8153-4658700(R) (A)
1456.75151456.8198-471021150(R) (G)
2089.87722089.9824-501251440(R) (A)
2105.89042105.9773-411Met-ox 1251440(R)ESTGAQVQVAGDMLPNSTER(A)
2160.04372160.1665-57pyroGlu 3263460(R)QVTITGSAASISLAQYLINAR(L)
2177.08062177.1930-523263460(R) (L)
2505.09802505.1940-381Met-ox 1782000(R)VMTIPYQPMPASSPVICAGGQDR(C)
2505.09802505.2255-511Met-ox 791010(K)LEEDINSSMTNSTAASRPPVTLR(L)
2521.08142521.1889-432Met-ox 1782000(R)VMTIPYQPMPASSPVICAGGQDR(C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 43% (155/356AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 11/80 matches (13%).
Acc. #: Species: HUMAN Name: Adseverin (Scinderin)
Index: MW: 80509 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1014.49981014.49683.02983061(K) (K)
1026.45741026.5161-573423481(K) (D)
1277.65861277.7469-693593691(K) (Q)
1316.57241316.5979-192512620(K) (V)
1449.73081449.7127134151(R) (Q)
1493.65691493.7276-471501620(R) (G)
1578.82151578.8029124344470(R) (D)
1847.86111847.9656-571471621(R) (G)
2160.04372159.982728pyroGlu 6967140(K)QGHEPPTFTGWFLGWDSSK(W)
2177.08062177.0092336967140(K) (W)
2225.01382225.0839-315976151(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (119/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/80 matches (12%).
Acc. #: Species: HUMAN Name: Zinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
Index: MW: 77888 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
997.4877997.4954-7.71021100(K) (I)
1008.52191008.4607612252311(R) (L)
1008.52191008.5326-1179871(R) (T)
1044.52761044.5478-194624700(R) (C)
1086.51911086.5696-475465540(R) (C)
1297.62381297.6435-152612700(R) (I)
1834.84021834.8580-9.76556691(R) (A)
1847.86111847.85334.23553691(R) (A)
1847.86111847.8645-1.93273411(R) (A)
2160.04372160.02568.41Met-ox 3703881(K)AFSQSSTLAQHQRMHTGEK(A)
2211.00642211.0756-311431610(K) (T)
2225.01382225.0304-7.46066240(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 22% (155/686AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/80 matches (10%).
Acc. #: Species: HUMAN Name: Zinc finger protein 274 (Zinc finger protein SP2114) (Zinc finger protein HFB101) (Zinc finger protein zfp2) (Zf2)
Index: MW: 74192 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1053.54581053.50394041480(R) (N)
1069.51621069.4988161Met-ox 41480(R)EVMLENYR(N)
1233.57651233.6089-261531631(K) (F)
1544.75281544.7934-265715831(R) (T)
1834.84021834.9088-372993131(R) (D)
1847.86111847.85334.25285420(K) (G)
2505.09802505.2336-543143350(R) (E)
2521.08142521.2285-581Met-ox 3143350(R)DVMLETFGHLVSVGWETTLENK(E)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 12% (84/653AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 14/80 matches (17%).
Acc. #: Species: MOUSE Name: Elongation factor 2 (EF-2)
Index: MW: 95315 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1017.48111017.4965-152402490(K) (A)
1055.57461055.5269451Met-ox 3924000(K)GPLMMYISK(M)
1063.53201063.5788-446686760(K) (D)
1069.51621069.502812pyroGlu 2272350(K)QFAEMYVAK(F)
1086.51911086.5294-9.52272350(K) (F)
1138.47411138.5169-386396470(K) (K)
1305.58851305.6473-4562711(K) (S)
1307.63711307.6530-1221320(R) (S)
1351.70751351.7585-385075191(R) (L)
1434.68971434.7341-312362491(K) (A)
1475.69971475.7901-611511621(R) (A)
1581.71231581.7947-525605721(K) (S)
1672.79881672.7749142Met-ox 3874001(K)SCDPKGPLMMYISK(M)
1691.77111691.8791-64AcetN 1151(-)VNFTVDQIRAIMDK(K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 15% (130/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 8/80 matches (10%).
Acc. #: Species: MOUSE Name: Dystrobrevin beta (Beta-dystrobrevin) (DTN-B) (MDTN-B)
Index: MW: 78357 Da pI: 8.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1053.54581053.4895531341430(K) (M)
1069.51621069.4844301Met-ox 1341430(K)AMLATMCGGK(M)
1383.65151383.7418-654724830(K) (L)
1493.65691493.7236-454594710(R) (A)
1691.77111691.8023-184905031(K) (R)
1707.70361707.7972-551Met-ox 4905031(K)DELEQRMSALQESR(R)
2045.03062045.01011048630(R) (D)
2225.01382225.0557-191481661(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (84/700AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 7/80 matches (8%).
Acc. #: Species: HUMAN Name: TRABID protein
Index: MW: 80908 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1008.52191008.4890332432500(K) (K)
1254.59831254.5789162232321(R) (I)
1295.62351295.6782-426726820(R) (W)
1838.82681838.792918881000(K) (A)
2105.89042105.9101-9.48230(K) (C)
2505.09802505.2668-676466661(K) (S)
2521.08142521.2617-721Met-ox 6466661(K)LLREWLDCCVTEGGVLVAMQK(S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (79/708AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 7/80 matches (8%).
Acc. #: Species: HUMAN Name: Neprilysin (Neutral endopeptidase) (NEP) (Enkephalinase) (Common acute lymphocytic leukemia antigen) (CALLA) (Neutral endopeptidase 24.11) (CD10)
Index: MW: 85515 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
997.4877997.5107-231041110(R) (D)
1012.45541012.4740-181781860(K) (A)
1284.57611284.6476-564264370(R) (H)
1449.73081449.6862314804920(R) (L)
1456.75151456.7072303974090(K) (R)
1838.82681838.8390-6.74104251(R) (L)
2045.03062044.93604669850(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (88/750AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 9/80 matches (11%).
Acc. #: Species: HUMAN Name: Mitochondrial 60s ribosomal protein L39 (L39mt) (MRP-L39) (MRP-L5) (PRED22 protein)
Index: MW: 34227 Da pI: 6.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1017.48111017.5158-342662730(R) (L)
1019.49801019.4872111471540(K) (E)
1037.51241037.5202-7.6181(-) (E)
1053.54581053.5151291Met-ox 181(-)MRNDLFNK(E)
1138.47411138.5567-731181260(K) (A)
1154.48601154.5516-571Met-ox 1181260(K)DEYMVNLVR(A)
1818.85621818.8197201031171(R) (D)
1834.84021834.8146141Met-ox 1031171(R)SCAMMMGCVIERAFK(D)
1850.83751850.8095152Met-ox 1031171(R)SCAMMMGCVIERAFK(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (48/296AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 9/80 matches (11%).
Acc. #: Species: HUMAN Name: Heat-shock protein 105 kDa (Heat shock 110 kDa protein) (Antigen NY-CO-25)
Index: MW: 96866 Da pI: 5.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1135.58311135.5855-2.26216291(K) (E)
1280.62291280.6487-20pyroGlu 1861960(K)QDLPSLDEKPR(I)
1297.62381297.6752-401861960(K) (I)
1388.75161388.7286177047141(K) (M)
1449.73081449.72990.61pyroGlu 6866971(K)QAYVDKLEELMK(I)
1475.69971475.7342-233613740(K) (G)
1818.85621818.9723-643753901(R) (E)
1834.84021834.9373-534845000(R) (V)
1850.83751850.9322-511Met-ox 4845000(R)VNTHGIFTISTASMVEK(V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (90/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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