MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D160
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 497 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/61(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.280e+01017 (27)41.027.98.2413.7 49542/7.3HUMANElongation factor Tu, mitochondrial precursor (P43)
238417 (11)20.011.515.650.6 43516/5.2MOUSESpectrin alpha chain, brain (Spectrin, non-erythroid alpha chain) (Alpha-II spectrin) (Fodrin alpha chain)
328325 (8)10.08.217.055.0 77888/8.7HUMANZinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
425796 (9)12.09.84.0130.4 65952/9.3HUMANHypothetical zinc finger protein KIAA1473
516697 (11)10.011.5-3.7252.8 90982/5.5HUMANDNA replication licensing factor MCM3 (DNA polymerase alpha holoenzyme-associated protein P1) (RLF beta subunit) (P102 protein) (P1-MCM3)
614765 (8)8.08.211.652.0 98472/4.5MOUSEEpidermal growth factor receptor substrate 15 (Protein Eps15) (AF-1p protein)
712424 (6)12.06.619.528.0 41523/6.1MOUSEGuanine nucleotide-binding protein, alpha-14 subunit
812025 (8)14.08.23.1658.5 50910/8.9HUMANIsocitrate dehydrogenase [NADP], mitochondrial precursor (Oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP) (ICD-M)
911816 (9)12.09.82.6053.0 57294/5.2HUMANHydroxymethylglutaryl-CoA synthase, cytoplasmic (HMG-CoA synthase) (3-hydroxy-3-methylglutaryl coenzyme A synthase)
1010817 (11)10.011.56.9677.5 85471/5.5MOUSEBeta-catenin
115838...........x......x...x....x.x.x...x.xx...x.xx...xx...xx.x...
104333..x................................xx.x..x...x..............x
47898.....................x.................x..........x.......xx.
105649.........................x.x.xxx.......................x.....
71737...x........x..x................x........x..x...........x....
108127......................x................x...x..........x....x.
23010..........................................x.........x.xx.....
82200x...................x.............x...............x......x...
72215........x...x................x..x.....x..................x...
26002...........x......x..............xx...................xx....x


Detailed Results

1. 17/61 matches (27%).
Acc. #: Species: HUMAN Name: Elongation factor Tu, mitochondrial precursor (P43)
Index: MW: 49542 Da pI: 7.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1032.57821032.56898.92722810(R) (G)
1099.56251099.5676-4.62012090(R) (G)
1149.66491149.6519113523610(K) (E)
1185.64911185.6228223163270(R) (G)
1277.66891277.65649.81Met-ox 3013110(R)TVVTGIEMFHK(S)
1364.62101364.597018921020(K) (A)
1466.79561466.8007-3.5pyroGlu 1701820(R)QIGVEHVVVYVNK(A)
1483.83961483.8233113163301(R) (R)
1483.83961483.82738.31701820(R) (A)
1492.70761492.692010911021(K) (A)
1542.87381542.8532132392520(K) (D)
1673.87451673.86118.01051200(R) (H)
1677.87831677.8812-1.73523661(K) (H)
1871.96971871.95378.62102270(K) (D)
1875.00871875.00502.01Met-ox 3964111(K)ELAMPGEDLKFNLILR(Q)
2046.01952046.00656.31832000(K) (E)
2062.02822062.0014131Met-ox 1832000(K)ADAVQDSEMVELVELEIR(E)
2129.18012129.16477.32532710(R) (G)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 41% (186/452AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/61 matches (11%).
Acc. #: Species: MOUSE Name: Spectrin alpha chain, brain (Spectrin, non-erythroid alpha chain) (Alpha-II spectrin) (Fodrin alpha chain)
Index: MW: 43516 Da pI: 5.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
966.4391966.4752-3771781(K) (K)
1466.79561466.7491322852970(R) (E)
1475.77491475.76476.91121(-) (V)
1492.70761492.6807182272381(R) (N)
1521.78971521.7372353003120(K) (R)
1677.87831677.8383243003131(K) (A)
2299.23982299.166932971170(K) (K)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 20% (80/382AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/61 matches (8%).
Acc. #: Species: HUMAN Name: Zinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
Index: MW: 77888 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1132.56241132.5751-115936010(R) (I)
1506.77491506.7930-124774890(K) (I)
1875.00871874.9257445185321(R) (A)
2211.12382211.0756221431610(K) (T)
2225.12372225.0304426066240(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 10% (75/686AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/61 matches (9%).
Acc. #: Species: HUMAN Name: Hypothetical zinc finger protein KIAA1473
Index: MW: 65952 Da pI: 9.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1171.65351171.64755.14844930(K) (Y)
1185.64911185.6632-124564650(K) (C)
1277.66891277.6490163043150(R) (I)
1307.68431307.6596193323430(K) (I)
1364.62101364.6446-172202310(K) (R)
2062.02822062.00031478941(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (73/574AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/61 matches (11%).
Acc. #: Species: HUMAN Name: DNA replication licensing factor MCM3 (DNA polymerase alpha holoenzyme-associated protein P1) (RLF beta subunit) (P102 protein) (P1-MCM3)
Index: MW: 90982 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
968.5561968.5165414965030(R) (M)
1034.54611034.53837.55485560(K) (K)
1060.55921060.6002-397377451(K) (A)
1378.63661378.6490-9.02712830(K) (I)
1521.78971521.8133-161Met-ox 2582701(R)TVLIACNVKQMSK(D)
1673.87451673.8532134644780(K) (F)
2076.00452076.0541-241591770(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (86/808AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 5/61 matches (8%).
Acc. #: Species: MOUSE Name: Epidermal growth factor receptor substrate 15 (Protein Eps15) (AF-1p protein)
Index: MW: 98472 Da pI: 4.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1149.66491149.6156437667760(K) (V)
1506.77491506.7804-3.73193330(K) (E)
1546.76431546.7349193673790(R) (E)
2046.01952045.9708241321500(K) (V)
2225.12372225.1778-243874051(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (77/897AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 4/61 matches (6%).
Acc. #: Species: MOUSE Name: Guanine nucleotide-binding protein, alpha-14 subunit
Index: MW: 41523 Da pI: 6.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1542.87381542.8128401041161(K) (V)
1940.95131940.9176173193340(K) (F)
2046.01952046.00477.22792950(K) (Q)
2062.02822061.9996141Met-ox 2792950(K)IMYSHLISYFPEYTGPK(Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (46/355AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 5/61 matches (8%).
Acc. #: Species: HUMAN Name: Isocitrate dehydrogenase [NADP], mitochondrial precursor (Oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP) (ICD-M)
Index: MW: 50910 Da pI: 8.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
955.4011955.4386-391731800(R) (A)
1112.50521112.5199-133003090(K) (N)
1437.90091437.8582301601720(R) (H)
1875.00871874.9877113613770(K) (G)
2129.18012129.1218272832991(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (65/452AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 6/61 matches (9%).
Acc. #: Species: HUMAN Name: Hydroxymethylglutaryl-CoA synthase, cytoplasmic (HMG-CoA synthase) (3-hydroxy-3-methylglutaryl coenzyme A synthase)
Index: MW: 57294 Da pI: 5.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1007.46761007.4330342322380(R) (K)
1034.54611034.5556-9.24014090(K) (S)
1277.66891277.6887-154014111(K) (L)
1378.63661378.6677-234164280(R) (L)
1492.70761492.7218-9.62782890(R) (D)
2129.18012129.0991385005201(K) (-)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (64/520AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 7/61 matches (11%).
Acc. #: Species: MOUSE Name: Beta-catenin
Index: MW: 85471 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1032.57821032.5400371Met-ox 1251330(R)LAEPSQMLK(H)
1099.56251099.5934-281Met-ox 1711800(K)AAVMVHQLSK(K)
1383.69501383.6439374754860(R) (L)
1437.90091437.8371444975080(K) (A)
2046.01952046.1026-414364531(K) (A)
2062.02822062.0975-341Met-ox 4364531(K)MMVCQVGGIEALVRTVLR(A)
2299.23982299.1644332933120(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (81/781AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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