MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D162
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 865 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/84(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.018e+00613 (15)31.015.52.3257.7 49542/7.3HUMANElongation factor Tu, mitochondrial precursor (P43)
22.895e+0049 (10)22.010.7-29.675.8 65952/9.3HUMANHypothetical zinc finger protein KIAA1473
31.489e+0047 (8)16.08.3-8.09102 30383/5.9HUMAN5'-AMP-activated protein kinase, beta-1 subunit (AMPK beta-1 chain) (AMPKb)
41.308e+0049 (10)15.010.715.974.1 73529/5.9MOUSEStress-70 protein, mitochondrial precursor (75 kDa glucose regulated protein) (GRP 75) (Peptide-binding protein 74) (PBP74) (P66 MOT) (Mortalin)
596446 (7)10.07.128.942.9 92470/4.8HUMANEndoplasmin precursor (94 kDa glucose-regulated protein) (GRP94) (gp96 homolog) (Tumor rejection antigen 1)
677568 (9)13.09.518.777.2 73681/5.9HUMANStress-70 protein, mitochondrial precursor (75 kDa glucose regulated protein) (GRP 75) (Peptide-binding protein 74) (PBP74) (Mortalin) (MOT)
770635 (5)14.06.07.3141.9 52619/5.9HUMANUbiquinol-cytochrome C reductase complex core protein I, mitochondrial precursor
849816 (7)14.07.16.7458.8 67639/5.6HUMANGigaxonin
947335 (5)10.06.07.06129 62617/6.0MOUSEGlypican-4 precursor (K-glypican)
1047176 (7)16.07.1-5.00132 44878/5.3HUMANRibonucleoside-diphosphate reductase M2 chain (Ribonucleotide reductase small chain)
115838...............................x............x......xx...x.x.x.x...xx....xx.........x
105649....x.......................x..x......x.....x.x....x........x......x................
75328....x..........................x..x..................x..x..................xx.......
32573..........x.....x...............................x....x........x.....xx.......x..x...
67245...................x.....................................x......x.x.xx..............
57265..........x.....x....................................x........x.....xx.......x..x...
51282....................................x...........x...................xx.............x
23588..................................x...x................x....x......x............x...
43698.........................x..................x.........................x.......xx....
96629....................................x.........x.x.............x.........xx..........


Detailed Results

1. 13/84 matches (15%).
Acc. #: Species: HUMAN Name: Elongation factor Tu, mitochondrial precursor (P43)
Index: MW: 49542 Da pI: 7.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1185.57871185.6228-373163270(R) (G)
1277.62181277.6564-271Met-ox 3013110(R)TVVTGIEMFHK(S)
1364.62921364.597024921020(K) (A)
1386.62861386.6687-292862971(K) (N)
1492.70991492.692012911021(K) (A)
1542.90431542.8532332392520(K) (D)
1644.88501644.8896-2.82983111(K) (S)
1790.95451791.0427-491Met-ox 3363511(R)RGLVMVKPGSIKPHQK(V)
2046.08692046.0065391832000(K) (E)
2062.02782062.0014131Met-ox 1832000(K)ADAVQDSEMVELVELEIR(E)
2129.21442129.1647232532710(R) (G)
2129.21442129.1647232532710(R) (G)
2858.40732858.38517.82Met-ox 1361620(K)NMITGTAPLDGCILVVAANDGPMPQTR(E)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 31% (144/452AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/84 matches (10%).
Acc. #: Species: HUMAN Name: Hypothetical zinc finger protein KIAA1473
Index: MW: 65952 Da pI: 9.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
881.3237881.3861-711861911(K) (S)
1177.50091177.5458-381171250(K) (K)
1185.57871185.6632-714564650(K) (C)
1228.60121228.6802-642882971(K) (C)
1228.60121228.6802-644284371(K) (C)
1277.62181277.6490-213043150(R) (I)
1307.61291307.6596-363323430(K) (I)
1364.62921364.6446-112202310(K) (R)
1644.88501644.8103451Met-ox 1922041(K)SFCMLSHLAQHKR(I)
1644.88501644.8961-6.73180(K) (D)
2062.02782062.00031378941(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (127/574AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/84 matches (8%).
Acc. #: Species: HUMAN Name: 5'-AMP-activated protein kinase, beta-1 subunit (AMPK beta-1 chain) (AMPKb)
Index: MW: 30383 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
881.3237881.3787-62180(-) (A)
1185.57871185.6050-222462560(K) (Y)
1201.51911201.5999-671Met-ox 2462560(K)DGVMVLSATHR(Y)
1421.70861421.6807201131(-) (H)
1492.70991492.7582-321Met-ox 2462581(K)DGVMVLSATHRYK(K)
2211.20282211.0835541081260(R) (F)
2211.20282211.0835541081260(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (45/270AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/84 matches (10%).
Acc. #: Species: MOUSE Name: Stress-70 protein, mitochondrial precursor (75 kDa glucose regulated protein) (GRP 75) (Peptide-binding protein 74) (PBP74) (P66 MOT) (Mortalin)
Index: MW: 73529 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
905.3852905.4216-406726781(K) (Q)
963.4138963.4457-335565630(K) (N)
1337.61421337.6225-6.21281381(R) (N)
1421.70861421.6582351Met-ox 5565671(K)DDIENMVKNAEK(Y)
1790.95451790.8781433623771(R) (S)
2092.16652092.04196053730(K) (Q)
2092.16652092.04196053730(K) (Q)
2233.13642233.1060146266461(K) (L)
2406.22952406.2009123693911(K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (102/679AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/84 matches (7%).
Acc. #: Species: HUMAN Name: Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94) (gp96 homolog) (Tumor rejection antigen 1)
Index: MW: 92470 Da pI: 4.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
982.5539982.4846711691770(K) (M)
1515.75991515.7120324754861(K) (E)
1878.99971878.9312361Met-ox 6096231(R)EAVEKEFEPLLNWMK(D)
1878.99971878.9430302712850(K) (T)
2046.08692046.0607131171350(R) (I)
2092.16652092.1451103200(R) (A)
2092.16652092.1451103200(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (88/803AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 8/84 matches (9%).
Acc. #: Species: HUMAN Name: Stress-70 protein, mitochondrial precursor (75 kDa glucose regulated protein) (GRP 75) (Peptide-binding protein 74) (PBP74) (Mortalin) (MOT)
Index: MW: 73681 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
905.3852905.4216-406726781(K) (Q)
963.4138963.4457-335565630(K) (N)
1421.70861421.6582351Met-ox 5565671(K)DDIENMVKNAEK(Y)
1790.95451790.8781433623771(R) (S)
2092.16652092.04196053730(K) (Q)
2092.16652092.04196053730(K) (Q)
2233.13642233.1060146266461(K) (L)
2406.22952406.2009123693911(K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (91/679AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 5/84 matches (5%).
Acc. #: Species: HUMAN Name: Ubiquinol-cytochrome C reductase complex core protein I, mitochondrial precursor
Index: MW: 52619 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1210.51421210.4832261711800(R) (D)
1337.61421337.6371-173793900(R) (G)
2092.16652092.1225212292480(R) (H)
2092.16652092.1225212292480(R) (H)
2858.40732858.4471-143974221(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (68/480AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 6/84 matches (7%).
Acc. #: Species: HUMAN Name: Gigaxonin
Index: MW: 67639 Da pI: 5.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1201.51911201.51662.14925000(K) (R)
1228.60121228.6360-282332420(R) (E)
1475.73131475.7681-254004111(K) (K)
1644.88501644.8532194454571(R) (R)
2062.02782061.9340453473640(K) (H)
2406.22952406.1651273653860(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (84/597AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 5/84 matches (5%).
Acc. #: Species: MOUSE Name: Glypican-4 precursor (K-glypican)
Index: MW: 62617 Da pI: 6.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1154.42381154.4909-582512580(K) (G)
1277.62181277.7074-674644741(R) (M)
2103.16362103.0189692592750(R) (G)
2300.25902300.1536463653840(R) (L)
2300.25902300.1536463653840(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (56/557AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 6/84 matches (7%).
Acc. #: Species: HUMAN Name: Ribonucleoside-diphosphate reductase M2 chain (Ribonucleotide reductase small chain)
Index: MW: 44878 Da pI: 5.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1210.51421210.5998-711Met-ox 3123210(K)LIGMNCTLMK(Q)
1307.61291307.6596-361491590(R) (C)
1337.61421337.7065-6962740(K) (E)
1790.95451790.9587-2.42492641(K) (D)
2129.21442129.05707480950(R) (K)
2129.21442129.05707480950(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (66/389AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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