MS-Fit Search Results



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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D164
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 1446 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score
#/105(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
15.626e+01216 (15)45.015.216.538.6 47169/7.0HUMANAlpha enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1) (Phosphopyruvate hydratase)
21.296e+0079 (8)33.08.613.749.1 47141/6.4MOUSEAlpha enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1)
32.777e+00611 (10)49.010.5-1.9354.5 34096/8.4HUMANCell division control protein 2 homolog (P34 protein kinase) (Cyclin-dependent kinase 1) (CDK1)
42.746e+0058 (7)25.07.67.6851.8 47025/6.7MOUSEBeta enolase (2-phospho-D-glycerate hydro-lyase) (Skeletal muscle enolase) (Enolase 3)
52.379e+0058 (7)21.07.63.0462.0 58927/5.6HUMANFormiminotransferase-cyclodeaminase (FTCD) (LCHC1) [Includes: Glutamate formiminotransferase (Glutamate formyltransferase); Formiminotetrahydrofolate cyclodeaminase ]
61.444e+0059 (8)14.08.6-4.6549.2 96950/8.2MOUSEDNA TOPOISOMERASE III BETA-1
71.433e+0058 (7)18.07.6-2.7567.9 59556/5.4MOUSET-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
81.183e+0058 (7)15.07.6-11.225.3 88073/5.0HUMANCadherin-18 precursor (Cadherin-14)
98.180e+0048 (7)37.07.65.3058.6 34107/8.4MOUSECell division control protein 2 homolog (P34 protein kinase) (Cyclin-dependent kinase 1) (CDK1)
106.938e+0048 (7)22.07.618.933.9 49478/5.8HUMANAlpha enolase, lung specific (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Phosphopyruvate hydratase) (HLE1)
60530.....x......x...........x.......xx..x.....x.......x.............xx...x....x......x...x........x..x.......
38648.....x......x...................x.........x.......x............x..........x......x............x..........
50125...........x.......................x.x....x..x...x......x..........x............x........x.........x.....
9758......................x....x....x...x...x.x.......x...........................................x..........
22320.........x........x....x...............x........................xx...................xx..................
53438............x....................x..x............x.x..x.................x.x...........x..................
84429............xx....x...................x................x.....x..............x..................x.........
84515x............x........................x............................x.x.....x.x..........x................
101787...................................x.x....x..x...x......x......x...................................x.....
25328............x...........x...........x...x.x....x...x......................x..............................



Detailed Results

1. 16/105 matches (15%).
Acc. #: Species: HUMAN Name: Alpha enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1) (Phosphopyruvate hydratase)
Index: MW: 47169 Da pI: 7.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1035.49021035.5331-413363430(K) (V)
1143.62521143.61627.91841930(R) (N)
1425.75451425.7266202702810(R) (S)
1533.83441533.8463-7.81061200(K) (A)
1540.81431540.7834202402530(K) (S)
1556.80051556.7783141Met-ox 2402530(K)VVIGMDVAASEFFR(S)
1647.88121647.8376263443580(K) (L)
1804.98721804.94452433500(R) (D)
1908.02571907.9876201631790(K) (E)
1923.99781923.98258.01Met-ox 1631790(K)LAMQEFMILPVGAANFR(E)
1940.01571939.9774202Met-ox 1631790(K)LAMQEFMILPVGAANFR(E)
1960.99401960.9252352032210(K) (E)
2033.10972033.0555273073260(K) (R)
2176.16832176.0749432342531(K) (S)
2367.22412367.1754213733940(R) (T)
2510.18592510.1152282863060(K) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 45% (197/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/105 matches (8%).
Acc. #: Species: MOUSE Name: Alpha enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1)
Index: MW: 47141 Da pI: 6.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1035.49021035.5331-413363430(K) (V)
1143.62521143.61627.91841930(R) (N)
1533.83441533.8463-7.81061200(K) (A)
1647.88121647.8376263443580(K) (L)
1804.98721804.94452433500(R) (D)
1897.03301896.9716321631790(K) (E)
1960.99401960.9252352032210(K) (E)
2033.10972033.0555273073260(K) (R)
2367.22412367.1754213733940(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 33% (144/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/105 matches (10%).
Acc. #: Species: HUMAN Name: Cell division control protein 2 homolog (P34 protein kinase) (Cyclin-dependent kinase 1) (CDK1)
Index: MW: 34096 Da pI: 8.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1122.54401122.5981-481Met-ox 2672751(K)MLIYDPAKR(I)
1555.79821555.8372-257201(K) (G)
1565.84181565.8116191591700(R) (S)
1647.88121647.87086.31Met-ox 76880(R)LYLIFEFLSMDLK(K)
1759.94701759.9708-1476891(R) (Y)
1801.94141801.92379.82012150(K) (I)
1852.00311851.93873560750(R) (L)
1932.98921932.9278322802950(K) (K)
1998.00731998.0271-9.91071220(K) (R)
2262.19372262.1692112552741(K) (R)
2705.25672705.3575-372162381(R) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 49% (147/297AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/105 matches (7%).
Acc. #: Species: MOUSE Name: Beta enolase (2-phospho-D-glycerate hydro-lyase) (Skeletal muscle enolase) (Enolase 3)
Index: MW: 47025 Da pI: 6.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1408.61391408.6821-482632751(K) (L)
1475.79061475.78583.34134261(R) (K)
1533.83441533.8463-7.81061200(K) (A)
1556.80051556.7783142402530(K) (N)
1572.81941572.7732291Met-ox 2402530(K)VVIGMDVAASEFYR(N)
1647.88121647.8376263443580(K) (L)
1804.98721804.94452433500(R) (D)
2367.22412367.1754213733940(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (111/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/105 matches (7%).
Acc. #: Species: HUMAN Name: Formiminotransferase-cyclodeaminase (FTCD) (LCHC1) [Includes: Glutamate formiminotransferase (Glutamate formyltransferase); Formiminotetrahydrofolate cyclodeaminase ]
Index: MW: 58927 Da pI: 5.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1055.54231055.5737-304254331(R) (D)
1307.69141307.6636212192290(K) (N)
1419.73861419.7120192892990(K) (I)
1570.86051570.85930.763213331(R) (G)
1908.02571907.9755261151310(R) (M)
1923.99781923.905748981140(R) (L)
2176.16832176.2593-422562760(R) (A)
2201.03772201.0800-194054240(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (119/541AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 9/105 matches (8%).
Acc. #: Species: MOUSE Name: DNA TOPOISOMERASE III BETA-1
Index: MW: 96950 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1143.62521143.61964.9911001(K) (F)
1540.81431540.8634-322963080(K) (V)
1556.80051556.8583-371Met-ox 2963080(K)QRPLALNTVEMLR(V)
1801.94141802.0097-381Met-ox 3190(K)TVLMVAEKPSLAQSIAK(I)
1809.89811809.87021554690(K) (Y)
1837.97961837.9852-3.12322460(K) (V)
1953.97771953.96397.13093270(R) (L)
1960.99401960.96561473891(K) (K)
2201.03772200.9803267467630(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (125/862AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 8/105 matches (7%).
Acc. #: Species: MOUSE Name: T-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
Index: MW: 59556 Da pI: 5.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1143.62521143.5686501721810(K) (L)
1173.59761173.6479-433683780(K) (G)
1307.69141307.63844121310(K) (N)
1568.74971568.7882-254915040(K) (Y)
1838.97201838.95747.91211370(R) (K)
1891.03341891.0475-7.55215390(R) (K)
1967.03761967.0523-7.51211381(R) (A)
2384.02162384.1114-382612811(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (104/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 8/105 matches (7%).
Acc. #: Species: HUMAN Name: Cadherin-18 precursor (Cadherin-14)
Index: MW: 88073 Da pI: 5.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
958.4600958.4846-266786860(R) (Y)
1173.59761173.6268-252302390(R) (D)
1568.74971568.7934-2840520(K) (R)
1932.98921932.98203.72722890(K) (I)
1940.01571940.0169-0.631972130(R) (T)
1961.97131961.9820-5.57017170(R) (Q)
1978.98761978.9987-5.63413571(K) (F)
2211.15202211.1602-3.75235401(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (119/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 8/105 matches (7%).
Acc. #: Species: MOUSE Name: Cell division control protein 2 homolog (P34 protein kinase) (Cyclin-dependent kinase 1) (CDK1)
Index: MW: 34107 Da pI: 8.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1555.79821555.8372-257201(K) (G)
1565.84181565.8116191591700(R) (S)
1647.88121647.87086.31Met-ox 76880(R)LYLIFEFLSMDLK(K)
1759.94701759.9708-1476891(R) (Y)
1801.94141801.92379.82012150(K) (I)
1852.00311851.93873560750(R) (L)
1897.03301896.941748901060(K) (S)
2705.25672705.3575-372162381(R) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 37% (111/297AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 8/105 matches (7%).
Acc. #: Species: HUMAN Name: Alpha enolase, lung specific (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Phosphopyruvate hydratase) (HLE1)
Index: MW: 49478 Da pI: 5.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1143.62521143.61627.91932020(R) (N)
1425.75451425.7266202792900(R) (G)
1556.80051556.7783142492620(K) (D)
1572.81941572.7732291Met-ox 2492620(K)VVIGMDVAASEFYR(D)
1647.88121647.8376263653790(K) (L)
1765.77971765.8076-161Met-ox 1721860(K)LAMQEFMIPPCGADR(F)
1809.89811809.836334961111(K) (F)
1960.99401960.9252352122300(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (101/458AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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