MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D166
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1095 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/78(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.990e+00618 (23)38.023.13.6939.1 43699/6.4MOUSEProliferation-associated protein 2G4 (Proliferation-associated protein 1) (Protein p38-2G4)
22.984e+00618 (23)38.023.13.6939.1 43787/6.1HUMANProliferation-associated protein 2G4 (Cell cycle protein p38-2G4 homolog) (hG4-1)
33.452e+0048 (10)16.010.36.1444.7 73244/6.7HUMANDEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
41.312e+0047 (8)13.09.01.8352.8 66695/5.7HUMANInterleukin-16 precursor (IL-16) (Lymphocyte chemoattractant factor) (LCF)
51.048e+0046 (7)10.07.7-3.7161.8 74442/8.3HUMANAcyl-coenzyme A oxidase 1, peroxisomal (Palmitoyl-CoA oxidase) (AOX)
21262x..........x...x.x.x..x.....x.xx.x...x.x..............x........xx.xx.........x
46967x..........x...x.x.x..x.....x.xx.x...x.x..............x........xx.xx.........x
3478.............x......................x.x...........x.....x...x..x...x..........
4150......................x.......xx.....x.......x........x...........x...........
33412............................x..........x...x.....x..............x.x...........


Detailed Results

1. 18/78 matches (23%).
Acc. #: Species: MOUSE Name: Proliferation-associated protein 2G4 (Proliferation-associated protein 1) (Protein p38-2G4)
Index: MW: 43699 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
984.4746984.4791-4.52642710(R) (R)
1060.59051060.6115-203563651(K) (T)
1097.55111097.54545.22732810(R) (A)
1113.53901113.5403-1.21Met-ox 2732810(R)FDAMPFTLR(A)
1124.54161124.5523-9.421301(K) (V)
1140.58141140.5472301Met-ox 21301(K)YKMGGDIANR(V)
1140.58141140.58021.12642721(R) (F)
1217.65681217.6425121Met-ox 23331(K)MGGDIANRVLR(S)
1253.63011253.6465-132722811(R) (A)
1269.64641269.64144.01Met-ox 2722811(R)RFDAMPFTLR(A)
1285.67161285.6752-2.82002100(K) (K)
1407.72671407.71299.92Met-ox 3213320(K)FTVLLMPNGPMR(I)
1413.71451413.7702-392002111(K) (D)
1926.99191927.0037-6.21561720(R) (V)
2161.07842161.014829AcetN 1200(-)SGEDEQQEQTIAEDLVVTK(Y)
2183.04592183.0742-131731910(K) (Q)
2199.11892199.0691231Met-ox 1731910(K)VAHSFNCTPIEGMLSHQLK(Q)
2208.14962208.0818311Met-ox 3453641(K)SEMEVQDAELKALLQSSASR(K)
2534.36662534.2787351Met-ox 2913111(R)MGVVECAKHELLQPFNVLYEK(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 38% (153/394AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 18/78 matches (23%).
Acc. #: Species: HUMAN Name: Proliferation-associated protein 2G4 (Cell cycle protein p38-2G4 homolog) (hG4-1)
Index: MW: 43787 Da pI: 6.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
984.4746984.4791-4.52642710(R) (R)
1060.59051060.6115-203563651(K) (T)
1097.55111097.54545.22732810(R) (A)
1113.53901113.5403-1.21Met-ox 2732810(R)FDAMPFTLR(A)
1124.54161124.5523-9.421301(K) (V)
1140.58141140.5472301Met-ox 21301(K)YKMGGDIANR(V)
1140.58141140.58021.12642721(R) (F)
1217.65681217.6425121Met-ox 23331(K)MGGDIANRVLR(S)
1253.63011253.6465-132722811(R) (A)
1269.64641269.64144.01Met-ox 2722811(R)RFDAMPFTLR(A)
1285.67161285.6752-2.82002100(K) (K)
1407.72671407.71299.92Met-ox 3213320(K)FTVLLMPNGPMR(I)
1413.71451413.7702-392002111(K) (D)
1926.99191927.0037-6.21561720(R) (V)
2161.07842161.014829AcetN 1200(-)SGEDEQQEQTIAEDLVVTK(Y)
2183.04592183.0742-131731910(K) (Q)
2199.11892199.0691231Met-ox 1731910(K)VAHSFNCTPIEGMLSHQLK(Q)
2208.14962208.0818311Met-ox 3453641(K)SEMEVQDAELKALLQSSASR(K)
2534.36662534.2787351Met-ox 2913111(R)MGVVECAKHELLQPFNVLYEK(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 38% (153/394AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/78 matches (10%).
Acc. #: Species: HUMAN Name: DEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
Index: MW: 73244 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1093.57391093.5530193433510(K) (M)
1320.66861320.6800-8.62772870(R) (K)
1408.70121408.6677242182301(K) (T)
1707.85661707.8964-233123261(R) (L)
1940.97541940.9792-1.93363511(K) (M)
2083.04122083.02497.85125280(K) (I)
2161.07842160.9813455655810(K) (G)
2208.14962208.1777-135355541(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (109/662AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/78 matches (8%).
Acc. #: Species: HUMAN Name: Interleukin-16 precursor (IL-16) (Lymphocyte chemoattractant factor) (LCF)
Index: MW: 66695 Da pI: 5.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1140.58141140.5425343553640(R) (E)
1253.63011253.62008.05395510(K) (G)
1269.64641269.6149251Met-ox 5395510(K)MSAGLGFSLEGGK(G)
1407.72671407.7708-315525640(K) (I)
1588.78601588.8335-304434570(R) (G)
1926.99191926.9561191401561(R) (G)
2199.11892199.1443-122432621(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (88/631AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/78 matches (7%).
Acc. #: Species: HUMAN Name: Acyl-coenzyme A oxidase 1, peroxisomal (Palmitoyl-CoA oxidase) (AOX)
Index: MW: 74442 Da pI: 8.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1217.65681217.7040-392923021(R) (Y)
1413.71451413.6949145265370(R) (L)
1508.84451508.8801-241831960(K) (G)
1698.86381698.7910433793930(K) (M)
2183.04592183.1031-261291480(K) (G)
2199.11892199.09819.51Met-ox 1291480(K)GLQIIGTYAQTEMGHGTHLR(G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (72/660AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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