MS-Fit Search Results

Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D167
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 1576 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/95(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	2.583e+010	16 (16)	28.0	16.8	-4.51	37.3	66018/8.2	HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
2	4.912e+008	16 (16)	36.0	16.8	5.20	30.4	61988/5.1	HUMAN	Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
3	1.046e+008	12 (12)	34.0	12.6	9.26	26.1	47169/7.0	HUMAN	Alpha enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1) (Phosphopyruvate hydratase)
4	2.740e+006	10 (10)	20.0	10.5	7.96	63.2	89615/8.6	HUMAN	Tumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
5	2.223e+005	7 (7)	17.0	7.4	-0.605	43.0	58914/5.5	HUMAN	Biotinidase precursor

48753	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	x	x	x	.	x	.	.	x	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.
104932	.	.	.	.	.	.	.	.	x	x	.	x	.	.	x	x	x	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	x	.	.	x	.	.	.	.	.	x
65205	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	x	x	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	x	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.
45962	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	x	.	x	.	.	.	.	.	.	.	.
87999	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.

Detailed Results

1. 16/95 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
973.5076	973.5318	-25		396	403	0	(K) (V)
1033.4995	1033.5166	-16		484	492	0	(R) (M)
1179.6070	1179.6010	5.1		377	386	0	(K) (H)
1265.6451	1265.6378	5.8		278	288	0	(R) (K)
1277.7156	1277.7105	4.0		473	483	0	(K) (T)
1300.5488	1300.5302	14		258	267	0	(K) (N)
1302.6669	1302.7018	-27		393	403	1	(R) (V)
1302.6669	1302.7157	-37		344	355	0	(R) (A)
1316.5313	1316.5251	4.7	1Met-ox	258	267	0	(K)NMQDMVEDYR(N)
1357.6685	1357.6963	-20		444	455	0	(K) (E)
1383.6836	1383.6909	-5.2		186	197	0	(K) (V)
1475.7702	1475.7494	14		212	223	0	(K) (T)
1475.7702	1475.7858	-11		200	211	0	(R) (W)
1638.7987	1638.8604	-38		186	199	1	(K) (F)
1699.8533	1699.8251	17	pyroGlu	418	432	0	(K)QISNLQQSISDAEQR(G)
1716.8582	1716.8517	3.8		418	432	0	(K) (G)
1993.9963	1993.9772	9.6		224	239	0	(R) (R)
2384.0013	2383.9524	20		519	549	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 28% (186/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 16/95 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1060.5358 1060.5639 -26 224 232 0 (K) (M)
1065.4930 1065.4999 -6.5 154 162 0 (K) (L)
1095.5417 1095.5475 -5.3 184 191 1 (K) (G)
1121.5852 1121.5843 0.84 327 335 0 (R) (N)
1140.5637 1140.5649 -1.1 pyroGlu 250 260 0 (R)QGVDADINGLR(Q)
1157.5803 1157.5915 -9.7 250 260 0 (R) (Q)
1307.6966 1307.6782 14 240 249 1 (R) (Q)
1323.6512 1323.6731 -17 1Met-ox 240 249 1 (R)IKFEMEQNLR(Q)
1791.7507 1791.7283 13 490 512 0 (R) (G)
1837.9760 1837.9660 5.4 374 389 0 (R) (K)
1851.9550 1851.9275 15 321 335 1 (K) (N)
2299.1670 2299.1202 20 271 289 1 (K) (N)
2377.2222 2377.1887 14 163 183 1 (R) (I)
2510.2002 2510.1323 27 449 471 0 (K) (I)
2705.2017 2705.1617 15 63 94 0 (R) (G)
3264.5946 3264.5144 25 339 367 0 (K) (E)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1060.5358	1060.5639	-26		224	232	0	(K) (M)
1065.4930	1065.4999	-6.5		154	162	0	(K) (L)
1095.5417	1095.5475	-5.3		184	191	1	(K) (G)
1121.5852	1121.5843	0.84		327	335	0	(R) (N)
1140.5637	1140.5649	-1.1	pyroGlu	250	260	0	(R)QGVDADINGLR(Q)
1157.5803	1157.5915	-9.7		250	260	0	(R) (Q)
1307.6966	1307.6782	14		240	249	1	(R) (Q)
1323.6512	1323.6731	-17	1Met-ox	240	249	1	(R)IKFEMEQNLR(Q)
1791.7507	1791.7283	13		490	512	0	(R) (G)
1837.9760	1837.9660	5.4		374	389	0	(R) (K)
1851.9550	1851.9275	15		321	335	1	(K) (N)
2299.1670	2299.1202	20		271	289	1	(K) (N)
2377.2222	2377.1887	14		163	183	1	(R) (I)
2510.2002	2510.1323	27		449	471	0	(K) (I)
2705.2017	2705.1617	15		63	94	0	(R) (G)
3264.5946	3264.5144	25		339	367	0	(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 36% (225/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 12/95 matches (12%).
Acc. #: Species: HUMAN Name: Alpha enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1) (Phosphopyruvate hydratase)
Index: MW: 47169 Da pI: 7.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1406.7095 1406.7167 -5.1 16 28 0 (R) (G)
1425.7270 1425.7266 0.30 270 281 0 (R) (S)
1525.7462 1525.7698 -15 359 372 0 (K) (S)
1540.8017 1540.7834 12 240 253 0 (K) (S)
1541.7744 1541.7647 6.3 1Met-ox 359 372 0 (K)LAQANGWGVMVSHR(S)
1556.7984 1556.7783 13 1Met-ox 240 253 0 (K)VVIGMDVAASEFFR(S)
1804.9663 1804.9445 12 33 50 0 (R) (D)
1923.9986 1923.9825 8.4 1Met-ox 163 179 0 (K)LAMQEFMILPVGAANFR(E)
1939.9934 1939.9774 8.2 2Met-ox 163 179 0 (K)LAMQEFMILPVGAANFR(E)
1960.9452 1960.9252 10 203 221 0 (K) (E)
2033.1116 2033.0555 28 307 326 0 (K) (R)
2510.2002 2510.1152 34 286 306 0 (K) (F)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Database Sequence
1406.7095	1406.7167	-5.1		16	28	(R) (G)
1425.7270	1425.7266	0.30		270	281	(R) (S)
1525.7462	1525.7698	-15		359	372	(K) (S)
1540.8017	1540.7834	12		240	253	(K) (S)
1541.7744	1541.7647	6.3	1Met-ox	359	372	(K)LAQANGWGVMVSHR(S)
1556.7984	1556.7783	13	1Met-ox	240	253	(K)VVIGMDVAASEFFR(S)
1804.9663	1804.9445	12		33	50	(R) (D)
1923.9986	1923.9825	8.4	1Met-ox	163	179	(K)LAMQEFMILPVGAANFR(E)
1939.9934	1939.9774	8.2	2Met-ox	163	179	(K)LAMQEFMILPVGAANFR(E)
1960.9452	1960.9252	10		203	221	(K) (E)
2033.1116	2033.0555	28		307	326	(K) (R)
2510.2002	2510.1152	34		286	306	(K) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 34% (148/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/95 matches (10%).
Acc. #: Species: HUMAN Name: Tumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
Index: MW: 89615 Da pI: 8.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1179.6070 1179.5581 42 677 685 0 (K) (F)
1406.7095 1406.7174 -5.6 707 718 1 (R) (A)
1493.7631 1493.6921 48 622 632 0 (K) (E)
1699.8533 1699.9343 -48 67 81 1 (K) (K)
1716.8582 1716.8267 18 288 301 1 (K) (L)
1851.9550 1851.9751 -11 421 439 0 (K) (G)
1993.9963 1994.0500 -27 25 41 0 (R) (T)
2307.1565 2307.1113 20 366 386 0 (R) (C)
2508.2346 2508.1288 42 163 183 1 (R) (S)
2532.3325 2532.3285 1.6 184 204 0 (R) (R)

m/z Submitted	MH⁺ Matched	Delta ppm	Start	End	Missed Cleavages	Database Sequence
1179.6070	1179.5581	42	677	685	0	(K) (F)
1406.7095	1406.7174	-5.6	707	718	1	(R) (A)
1493.7631	1493.6921	48	622	632	0	(K) (E)
1699.8533	1699.9343	-48	67	81	1	(K) (K)
1716.8582	1716.8267	18	288	301	1	(K) (L)
1851.9550	1851.9751	-11	421	439	0	(K) (G)
1993.9963	1994.0500	-27	25	41	0	(R) (T)
2307.1565	2307.1113	20	366	386	0	(R) (C)
2508.2346	2508.1288	42	163	183	1	(R) (S)
2532.3325	2532.3285	1.6	184	204	0	(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (160/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/95 matches (7%).
Acc. #: Species: HUMAN Name: Biotinidase precursor
Index: MW: 58914 Da pI: 5.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1121.5852 1121.5591 23 129 137 0 (R) (L)
1265.6451 1265.6564 -8.9 145 156 0 (R) (E)
1307.6966 1307.7323 -27 506 518 0 (R) (L)
1583.7871 1583.8143 -17 341 353 1 (K) (D)
2093.9954 2094.0104 -7.1 320 340 0 (K) (F)
2807.4146 2807.3232 33 pyroGlu 1Met-ox 60 83 0 (R)QEALELMNQNLDIYEQQVMTAAQK(D)
2808.3562 2808.3548 0.50 60 83 0 (R) (D)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1121.5852	1121.5591	23		129	137	0	(R) (L)
1265.6451	1265.6564	-8.9		145	156	0	(R) (E)
1307.6966	1307.7323	-27		506	518	0	(R) (L)
1583.7871	1583.8143	-17		341	353	1	(K) (D)
2093.9954	2094.0104	-7.1		320	340	0	(K) (F)
2807.4146	2807.3232	33	pyroGlu 1Met-ox	60	83	0	(R)QEALELMNQNLDIYEQQVMTAAQK(D)
2808.3562	2808.3548	0.50		60	83	0	(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (92/523AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):