MS-Fit Search Results

Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D169
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1135 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/100(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	3.200e+009	18 (18)	39.0	18.0	-18.6	26.4	58025/6.2	HUMAN	T-complex protein 1, zeta subunit (TCP-1-zeta) (CCT-zeta) (CCT-zeta-1) (Tcp20) (HTR3)
2	1.423e+008	16 (16)	34.0	16.0	-16.3	25.1	58005/6.6	MOUSE	T-complex protein 1, zeta subunit (TCP-1-zeta) (CCT-zeta) (CCT-zeta-1)
3	7.243e+006	15 (15)	26.0	15.0	-9.78	59.7	61988/5.1	HUMAN	Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
4	4.135e+006	13 (13)	29.0	13.0	-11.0	47.0	66018/8.2	HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
5	4.811e+004	7 (7)	14.0	7.0	1.35	45.5	58185/7.0	MOUSE	T-complex protein 1, zeta-2 subunit (TCP-1-zeta-2) (CCT-zeta-2) (Cctz-2)

32957	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	x	.	.	.	x	x	x	x	.	x	.	x	x	.	.	.	x	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	x	x	.	.	x	.	.
92349	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	x	.	x	x	.	x	.	x	x	.	.	.	x	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	x	.	.	x	.	.
100557	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	x	.	x	.	.	.	x	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	x	.
46726	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	x	x	.	x	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	x	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.
30181	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	x	x	.	.	.	.	.

Detailed Results

1. 18/100 matches (18%).
Acc. #: Species: HUMAN Name: T-complex protein 1, zeta subunit (TCP-1-zeta) (CCT-zeta) (CCT-zeta-1) (Tcp20) (HTR3)
Index: MW: 58025 Da pI: 6.2

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1255.6754	1255.7123	-29		16	28	0	(R) (G)
1262.6043	1262.6632	-47		296	307	0	(K) (E)
1335.6821	1335.7160	-25		118	129	1	(R) (A)
1407.6655	1407.7340	-49		42	55	1	(K) (L)
1418.7760	1418.8007	-17		130	141	1	(K) (E)
1447.7041	1447.7215	-12		142	153	1	(R) (T)
1463.6809	1463.7164	-24	1Met-ox	142	153	1	(R)EMDRETLIDVAR(T)
1481.7116	1481.7389	-18	pyroGlu	105	117	0	(K)QADLYISEGLHPR(I)
1498.7395	1498.7654	-17		105	117	0	(K) (I)
1561.7744	1561.7909	-11	1Met-ox	209	222	1	(R)GLVLDHGARHPDMK(K)
1679.8155	1679.8427	-16		281	295	1	(K) (G)
1761.9119	1761.9387	-15		450	465	0	(K) (I)
1781.9601	1781.9986	-22		11	28	1	(K) (G)
1981.1389	1981.1599	-11		431	449	1	(K) (V)
2001.0857	2001.1021	-8.2		296	314	1	(K) (R)
2315.1880	2315.2182	-13		59	79	0	(K) (V)
2331.2050	2331.2131	-3.5	1Met-ox	59	79	0	(K)DGNVLLHEMQIQHPTASLIAK(V)
2544.3503	2544.3409	3.7		80	104	0	(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 39% (210/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 16/100 matches (16%).
Acc. #: Species: MOUSE Name: T-complex protein 1, zeta subunit (TCP-1-zeta) (CCT-zeta) (CCT-zeta-1)
Index: MW: 58005 Da pI: 6.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
864.4259 864.4249 1.2 139 145 1 (K) (E)
1255.6754 1255.7123 -29 16 28 0 (R) (G)
1335.6821 1335.7160 -25 118 129 1 (R) (A)
1407.6655 1407.7340 -49 42 55 1 (K) (L)
1447.7041 1447.7215 -12 142 153 1 (K) (T)
1463.6809 1463.7164 -24 1Met-ox 142 153 1 (K)EMDRETLIDVAR(T)
1481.7116 1481.7389 -18 pyroGlu 105 117 0 (K)QADLYISEGLHPR(I)
1498.7395 1498.7654 -17 105 117 0 (K) (I)
1561.7744 1561.7909 -11 1Met-ox 209 222 1 (R)GLVLDHGARHPDMK(K)
1679.8155 1679.8427 -16 281 295 1 (K) (G)
1761.9119 1761.9387 -15 450 465 0 (K) (V)
1781.9601 1781.9986 -22 11 28 1 (K) (G)
1981.1389 1981.1599 -11 431 449 1 (K) (V)
2315.1880 2315.2182 -13 59 79 0 (K) (V)
2331.2050 2331.2131 -3.5 1Met-ox 59 79 0 (K)DGNVLLHEMQIQHPTASLIAK(V)
2544.3503 2544.3409 3.7 80 104 0 (K) (Q)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
864.4259	864.4249	1.2		139	145	1	(K) (E)
1255.6754	1255.7123	-29		16	28	0	(R) (G)
1335.6821	1335.7160	-25		118	129	1	(R) (A)
1407.6655	1407.7340	-49		42	55	1	(K) (L)
1447.7041	1447.7215	-12		142	153	1	(K) (T)
1463.6809	1463.7164	-24	1Met-ox	142	153	1	(K)EMDRETLIDVAR(T)
1481.7116	1481.7389	-18	pyroGlu	105	117	0	(K)QADLYISEGLHPR(I)
1498.7395	1498.7654	-17		105	117	0	(K) (I)
1561.7744	1561.7909	-11	1Met-ox	209	222	1	(R)GLVLDHGARHPDMK(K)
1679.8155	1679.8427	-16		281	295	1	(K) (G)
1761.9119	1761.9387	-15		450	465	0	(K) (V)
1781.9601	1781.9986	-22		11	28	1	(K) (G)
1981.1389	1981.1599	-11		431	449	1	(K) (V)
2315.1880	2315.2182	-13		59	79	0	(K) (V)
2331.2050	2331.2131	-3.5	1Met-ox	59	79	0	(K)DGNVLLHEMQIQHPTASLIAK(V)
2544.3503	2544.3409	3.7		80	104	0	(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 34% (182/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 15/100 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
897.3694 897.4140 -50 233 239 0 (R) (I)
1060.5156 1060.5639 -46 224 232 0 (K) (M)
1065.4708 1065.4999 -27 154 162 0 (K) (L)
1138.6368 1138.5931 38 233 241 1 (R) (F)
1157.5492 1157.5915 -36 250 260 0 (R) (Q)
1235.5132 1235.5293 -13 46 58 0 (R) (V)
1307.6419 1307.6782 -28 240 249 1 (R) (Q)
1323.6136 1323.6731 -45 1Met-ox 240 249 1 (R)IKFEMEQNLR(Q)
1791.7198 1791.7283 -4.7 490 512 0 (R) (G)
1837.9459 1837.9660 -11 374 389 0 (R) (K)
1851.9193 1851.9275 -4.4 321 335 1 (K) (N)
1867.9276 1867.9224 2.8 1Met-ox 321 335 1 (K)TLNDMRQEYEQLIAK(N)
2315.1880 2315.1151 31 1Met-ox 271 289 1 (K)SDLEMQYETLQEELMALKK(N)
2331.2050 2331.1100 41 2Met-ox 271 289 1 (K)SDLEMQYETLQEELMALKK(N)
2705.1741 2705.1617 4.6 63 94 0 (R) (G)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
897.3694	897.4140	-50		233	239	0	(R) (I)
1060.5156	1060.5639	-46		224	232	0	(K) (M)
1065.4708	1065.4999	-27		154	162	0	(K) (L)
1138.6368	1138.5931	38		233	241	1	(R) (F)
1157.5492	1157.5915	-36		250	260	0	(R) (Q)
1235.5132	1235.5293	-13		46	58	0	(R) (V)
1307.6419	1307.6782	-28		240	249	1	(R) (Q)
1323.6136	1323.6731	-45	1Met-ox	240	249	1	(R)IKFEMEQNLR(Q)
1791.7198	1791.7283	-4.7		490	512	0	(R) (G)
1837.9459	1837.9660	-11		374	389	0	(R) (K)
1851.9193	1851.9275	-4.4		321	335	1	(K) (N)
1867.9276	1867.9224	2.8	1Met-ox	321	335	1	(K)TLNDMRQEYEQLIAK(N)
2315.1880	2315.1151	31	1Met-ox	271	289	1	(K)SDLEMQYETLQEELMALKK(N)
2331.2050	2331.1100	41	2Met-ox	271	289	1	(K)SDLEMQYETLQEELMALKK(N)
2705.1741	2705.1617	4.6		63	94	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (164/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 13/100 matches (13%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1157.5492 1157.5149 30 1Met-ox 464 472 0 (R)DYQELMNTK(L)
1179.5736 1179.6010 -23 377 386 0 (K) (H)
1265.5910 1265.6378 -37 278 288 0 (R) (K)
1302.6678 1302.7018 -26 393 403 1 (R) (V)
1302.6678 1302.7157 -37 344 355 0 (R) (A)
1316.5772 1316.5251 40 1Met-ox 258 267 0 (K)NMQDMVEDYR(N)
1393.6798 1393.7327 -38 278 289 1 (R) (D)
1475.7405 1475.7494 -6.0 212 223 0 (K) (T)
1475.7405 1475.7858 -31 200 211 0 (R) (W)
1638.8343 1638.8604 -16 186 199 1 (K) (F)
1657.7889 1657.7934 -2.7 13 29 0 (R) (R)
1687.9489 1687.9707 -13 66 82 1 (R) (G)
1993.9459 1993.9772 -16 224 239 0 (R) (R)
2286.1372 2286.1254 5.2 367 386 1 (K) (H)
2383.9661 2383.9524 5.7 519 549 0 (R) (G)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1157.5492	1157.5149	30	1Met-ox	464	472	0	(R)DYQELMNTK(L)
1179.5736	1179.6010	-23		377	386	0	(K) (H)
1265.5910	1265.6378	-37		278	288	0	(R) (K)
1302.6678	1302.7018	-26		393	403	1	(R) (V)
1302.6678	1302.7157	-37		344	355	0	(R) (A)
1316.5772	1316.5251	40	1Met-ox	258	267	0	(K)NMQDMVEDYR(N)
1393.6798	1393.7327	-38		278	289	1	(R) (D)
1475.7405	1475.7494	-6.0		212	223	0	(K) (T)
1475.7405	1475.7858	-31		200	211	0	(R) (W)
1638.8343	1638.8604	-16		186	199	1	(K) (F)
1657.7889	1657.7934	-2.7		13	29	0	(R) (R)
1687.9489	1687.9707	-13		66	82	1	(R) (G)
1993.9459	1993.9772	-16		224	239	0	(R) (R)
2286.1372	2286.1254	5.2		367	386	1	(K) (H)
2383.9661	2383.9524	5.7		519	549	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 29% (193/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/100 matches (7%).
Acc. #: Species: MOUSE Name: T-complex protein 1, zeta-2 subunit (TCP-1-zeta-2) (CCT-zeta-2) (Cctz-2)
Index: MW: 58185 Da pI: 7.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1060.5156 1060.5097 5.5 pyroGlu 279 287 1 (K)QKVCAESNK(G)
1481.7116 1481.7389 -18 pyroGlu 105 117 0 (K)QADLYISEGLHPR(I)
1498.7395 1498.7654 -17 105 117 0 (K) (I)
1981.1389 1981.0467 47 200 217 1 (K) (H)
2001.0857 2001.0665 9.6 181 197 0 (R) (H)
2315.1880 2315.2182 -13 59 79 0 (K) (V)
2331.2050 2331.2131 -3.5 1Met-ox 59 79 0 (K)DGNVLLHEMQIQHPTASIIAK(V)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1060.5156	1060.5097	5.5	pyroGlu	279	287	1	(K)QKVCAESNK(G)
1481.7116	1481.7389	-18	pyroGlu	105	117	0	(K)QADLYISEGLHPR(I)
1498.7395	1498.7654	-17		105	117	0	(K) (I)
1981.1389	1981.0467	47		200	217	1	(K) (H)
2001.0857	2001.0665	9.6		181	197	0	(R) (H)
2315.1880	2315.2182	-13		59	79	0	(K) (V)
2331.2050	2331.2131	-3.5	1Met-ox	59	79	0	(K)DGNVLLHEMQIQHPTASIIAK(V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (78/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):