MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D170
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1095 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/91(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.084e+01425 (27)53.027.5-7.2845.5 48634/5.7HUMAN26S protease regulatory subunit 7 (MSS1 protein)
22.083e+01425 (27)53.027.5-7.2845.5 48648/5.7MOUSE26S protease regulatory subunit 7 (MSS1 protein)
358857 (7)25.07.70.47765.6 50664/6.1HUMANRab GDP dissociation inhibitor beta (Rab GDI beta) (GDI-2)
4584410 (10)21.011.0-3.1046.1 65447/6.4MOUSELamins C and C2
5391710 (10)18.011.0-3.1046.1 74210/6.5MOUSELamin A
3617x..x............................x....x.x.x..x.x.x....x.xx.xx.xx....xx...x...xxx......xx..x.
98534x..x............................x....x.x.x..x.x.x....x.xx.xx.xx....xx...x...xxx......xx..x.
17294...........x.............................................x....x...........x..x...x.....x...
113067...............x................................x..x...x..........x....x.x..x........xx....
14532...............x................................x..x...x..........x....x.x..x........xx....


Detailed Results

1. 25/91 matches (27%).
Acc. #: Species: HUMAN Name: 26S protease regulatory subunit 7 (MSS1 protein)
Index: MW: 48634 Da pI: 5.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
896.3994896.4300-342332390(R) (V)
911.3877911.4297-461Met-ox 2592650(R)ELFEMAR(T)
1062.46161062.4856-2335430(K) (Q)
1132.61681132.6479-273673751(R) (L)
1142.61331142.6574-392112220(K) (T)
1157.59231157.6418-4324340(R) (T)
1205.68251205.7047-181471560(K) (I)
1231.59281231.6105-143763860(R) (S)
1275.65051275.6585-6.33413510(K) (T)
1328.66371328.7062-3247570(K) (K)
1350.65921350.6152334234331(K) (-)
1355.64091355.6452-3.13873980(R) (A)
1371.62971371.6401-7.61Met-ox 3873980(R)SVCTEAGMFAIR(A)
1379.58031379.5828-1.82852970(R) (T)
1403.74641403.7535-5.03403511(R) (T)
1418.75581418.7644-6.01892000(R) (F)
1480.75321480.7760-15pyroGlu 85970(K)QTLQSEQPLQVAR(C)
1497.79311497.8025-6.385970(K) (C)
1687.96321687.94958.11872001(K) (F)
1761.87641761.8845-4.62983120(R) (G)
1777.91901777.8795221Met-ox 2983120(R)TMLELINQLDGFDPR(G)
1781.90861781.8896112692840(K) (F)
2121.05642121.0174181211390(K) (V)
2137.06312137.0123241Met-ox 1211390(K)FVVDLSDQVAPTDIEEGMR(V)
2256.23752256.1593352Met-ox 3173360(K)VLMATNRPDTLDPALMRPGR(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 53% (232/433AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 25/91 matches (27%).
Acc. #: Species: MOUSE Name: 26S protease regulatory subunit 7 (MSS1 protein)
Index: MW: 48648 Da pI: 5.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
896.3994896.4300-342332390(R) (V)
911.3877911.4297-461Met-ox 2592650(R)ELFEMAR(T)
1062.46161062.4856-2335430(K) (Q)
1132.61681132.6479-273673751(R) (L)
1142.61331142.6574-392112220(K) (T)
1157.59231157.6418-4324340(R) (T)
1205.68251205.7047-181471560(K) (I)
1231.59281231.6105-143763860(R) (S)
1275.65051275.6585-6.33413510(K) (T)
1328.66371328.7062-3247570(K) (K)
1350.65921350.6152334234331(K) (-)
1355.64091355.6452-3.13873980(R) (A)
1371.62971371.6401-7.61Met-ox 3873980(R)SVCTEAGMFAIR(A)
1379.58031379.5828-1.82852970(R) (T)
1403.74641403.7535-5.03403511(R) (T)
1418.75581418.7644-6.01892000(R) (F)
1480.75321480.7760-15pyroGlu 85970(K)QTLQSEQPLQVAR(C)
1497.79311497.8025-6.385970(K) (C)
1687.96321687.94958.11872001(K) (F)
1761.87641761.8845-4.62983120(R) (G)
1777.91901777.8795221Met-ox 2983120(R)TMLELINQLDGFDPR(G)
1781.90861781.8896112692840(K) (F)
2121.05642121.0174181211390(K) (V)
2137.06312137.0123241Met-ox 1211390(K)FVVDLSDQVAPTDIEEGMR(V)
2256.23752256.1593352Met-ox 3173360(K)VLMATNRPDTLDPALMRPGR(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 53% (232/433AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/91 matches (7%).
Acc. #: Species: HUMAN Name: Rab GDP dissociation inhibitor beta (Rab GDI beta) (GDI-2)
Index: MW: 50664 Da pI: 6.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
953.3869953.4216-364384451(K) (-)
1365.68041365.7014-153914020(K) (T)
1418.75581418.7102321Met-ox 56681(R)FKIPGSPPESMGR(G)
1712.87911712.86488.31431561(K) (T)
1777.91901778.0064-493653790(K) (F)
1916.85441916.8336111942080(R) (I)
2141.11872141.0160481Met-ox 3303480(K)SDIYVCMISFAHNVAAQGK(Y)
2141.11872141.10725.42222400(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (115/445AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/91 matches (10%).
Acc. #: Species: MOUSE Name: Lamins C and C2
Index: MW: 65447 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
972.5065972.5114-5.13893970(R) (S)
1275.65051275.6909-321451561(K) (T)
1320.59061320.6409-384284391(R) (V)
1350.65921350.6225275165270(K) (T)
1475.75381475.7681-9.61Met-ox 3673781(K)LALDMEIHAYRK(L)
1604.81611604.7768254364501(R) (F)
1707.80491707.8050-0.114214351(K) (T)
1761.87641761.827028pyroGlu 4724861(R)QNGDDPLMTYRFPPK(F)
1761.87641761.9234-2763781(R) (A)
2121.05642121.0650-4.03503661(R) (L)
2137.06312137.05991.51Met-ox 3503661(R)ARMQQQLDEYQELLDIK(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (123/574AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/91 matches (10%).
Acc. #: Species: MOUSE Name: Lamin A
Index: MW: 74210 Da pI: 6.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
972.5065972.5114-5.13893970(R) (S)
1275.65051275.6909-321451561(K) (T)
1320.59061320.6409-384284391(R) (V)
1350.65921350.6225275165270(K) (T)
1475.75381475.7681-9.61Met-ox 3673781(K)LALDMEIHAYRK(L)
1604.81611604.7768254364501(R) (F)
1707.80491707.8050-0.114214351(K) (T)
1761.87641761.827028pyroGlu 4724861(R)QNGDDPLMTYRFPPK(F)
1761.87641761.9234-2763781(R) (A)
2121.05642121.0650-4.03503661(R) (L)
2137.06312137.05991.51Met-ox 3503661(R)ARMQQQLDEYQELLDIK(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (123/665AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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