MS-Fit Search Results


.

Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D171
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 3312 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/126(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.187e+01426 (20)56.020.699.719.0 57489/6.0HUMANT-complex protein 1, beta subunit (TCP-1-beta) (CCT-beta)
21.574e+01224 (19)53.019.098.623.0 57448/6.0MOUSET-complex protein 1, beta subunit (TCP-1-beta) (CCT-beta)
31.400e+00611 (8)20.08.767.496.3 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
42.471e+00511 (8)19.08.772.7113 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
52.221e+0058 (6)16.06.334.4130 73103/6.1MOUSEKininogen precursor [Contains: Bradykinin]
62.187e+00514 (11)18.011.131.2162 90360/5.0HUMANTransforming acidic coiled-coil-containing protein 3
71.724e+0059 (7)15.07.167.883.4 85941/6.1MOUSESignal transducer and activator of transcription 4
81.411e+00510 (7)25.07.950.9112 51673/8.1HUMANAdenylyl cyclase-associated protein 1 (CAP 1)
91.408e+0058 (6)39.06.339.1181 34096/8.4HUMANCell division control protein 2 homolog (P34 protein kinase) (Cyclin-dependent kinase 1) (CDK1)
101.115e+0058 (6)17.06.366.1109 59519/5.1HUMANKeratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
62497........................x................xx.x.x......x........x.x.xxx....x...x....x....x...xxxx.x........x....x.x....x.....xx.
24337........................x.................x.x.x........x........xxxxx...xx...x....x....x.....xx.x........x....x.x....x.....xx.
97443.......................x..x.....x...........x........................x.........x.x.................................x.xxx......
45313.........x........x......xx.x..........x............x......x..........x.................x........x............................
85222...x.......................x.x......x......x.............................................................x..x...........x.....
38478...x..x.......x...x..................x...x..x.......x.x....x.....x.............x.x.........................................x..
49076......................x..x....................x.................x.x..x...............x...x.........................x..........
101584................x....x.....x........x.....................................x..........xx.x......................x.....x........
50125x.............................................x.............................x..x..x.......x..x.........................x......
108237................x..........x..........................x......x.........x....x............x.......................x............


Detailed Results

1. 26/126 matches (20%).
Acc. #: Species: HUMAN Name: T-complex protein 1, beta subunit (TCP-1-beta) (CCT-beta)
Index: MW: 57489 Da pI: 6.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1144.66601144.5686852852930(K) (Q)
1291.85071291.7275951211310(K) (E)
1301.86271301.746989AcetN 1130(-)ASLSLAPVNIFK(A)
1307.81511307.65171251Met-ox 2372480(K)ILIANTGMDTDK(I)
1330.78901330.6603973773880(R) (S)
1419.96011419.8225971201311(K) (E)
1518.03391517.89439226400(R) (S)
1548.99791548.83071081Met-ox 2372501(K)ILIANTGMDTDKIK(I)
1566.04191565.890397pyroGlu 5025160(R)QVLLSAAEAAEVILR(V)
1568.99201568.8470923894020(R) (D)
1583.07421582.9168995025160(R) (V)
1739.17541739.0179915015161(K) (V)
1799.11061798.94859073891(K) (V)
1913.14351912.9544992052220(K) (K)
1970.29061970.08971023593760(K) (G)
2009.19892009.0265864825000(R) (R)
2025.24522025.02141101Met-ox 4825000(R)EGTIGDMAILGITESFQVK(R)
2041.26362041.04941052042221(K) (K)
2041.26362041.04941052052231(K) (I)
2097.34552097.12321063233420(R) (L)
2119.30422119.08581031201(-) (A)
2288.40862288.1622108901110(R) (E)
2347.44322347.19681054454660(R) (A)
2363.46942363.19171181Met-ox 4454660(R)MLPTIIADNAGYDSADLVAQLR(A)
2396.33282396.1161901Met-ox 4064270(R)TVYGGGCSEMLMAHAVTQLANR(T)
3208.87693208.5414105pyroGlu 1Met-ox 2943220(R)QLIYNYPEQLFGAAGVMAIEHADFAGVER(L)
3225.87193225.5679941Met-ox 2943220(R)QLIYNYPEQLFGAAGVMAIEHADFAGVER(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 56% (301/535AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 24/126 matches (19%).
Acc. #: Species: MOUSE Name: T-complex protein 1, beta subunit (TCP-1-beta) (CCT-beta)
Index: MW: 57448 Da pI: 6.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1144.66601144.5686852852930(K) (Q)
1301.86271301.746989AcetN 1130(-)ASLSLAPVNIFK(A)
1307.81511307.65171251Met-ox 2372480(K)ILIANTGMDTDK(I)
1307.81511307.7224711211310(K) (E)
1330.78901330.6603973773880(R) (S)
1435.93001435.8174781201311(K) (E)
1548.99791548.83071081Met-ox 2372501(K)ILIANTGMDTDKIK(I)
1553.88531553.7382951Met-ox 4674810(R)AAHSEGHITAGLDMK(E)
1566.04191565.890397pyroGlu 5025160(R)QVLLSAAEAAEVILR(V)
1568.99201568.8470923894020(R) (D)
1583.07421582.9168995025160(R) (V)
1737.13341736.94481091211351(K) (A)
1739.17541739.0179915015161(K) (V)
1799.11061798.94859073891(K) (V)
1913.14351912.9544992052220(K) (K)
1970.29061970.08971023593760(K) (G)
2041.26362041.04941052042221(K) (K)
2041.26362041.04941052052231(K) (I)
2097.34552097.12321063233420(R) (L)
2119.30422119.08581031201(-) (A)
2288.40862288.1622108901110(R) (E)
2347.44322347.19681054454660(R) (A)
2363.46942363.19171181Met-ox 4454660(R)MLPTIIADNAGYDSADLVAQLR(A)
2396.33282396.1161901Met-ox 4064270(R)TVYGGGCSEMLMAHAVTQLANR(T)
3208.87693208.5414105pyroGlu 1Met-ox 2943220(R)QLIYNYPEQLFGAAGVMAIEHADFAGVER(L)
3225.87193225.5679941Met-ox 2943220(R)QLIYNYPEQLFGAAGVMAIEHADFAGVER(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 53% (286/535AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/126 matches (8%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1140.50221140.5649-55pyroGlu 2502600(R)QGVDADINGLR(Q)
1157.63801157.5915402502600(R) (Q)
1235.63041235.52938246580(R) (V)
1307.81511307.67821052402491(R) (Q)
1605.85771605.7437711992110(K) (D)
1852.09811851.9275923213351(K) (N)
1871.98671871.9173371Met-ox 1541691(K)STMQELNSRLASYLDK(V)
2379.42912379.1688109pyroGlu 4274451(R)QEIECQNQEYSLLLSIKMR(L)
2396.33282396.1954574274451(R) (L)
2412.41512412.1903931Met-ox 4274451(R)QEIECQNQEYSLLLSIKMR(L)
2705.45822705.161711063940(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (129/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 11/126 matches (8%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1016.43661016.5013-643563640(K) (S)
1125.51381125.5428-263673760(K) (Y)
1152.64451152.5537792682761(R) (R)
1157.63801157.51491061Met-ox 4644720(R)DYQELMNTK(L)
1179.71581179.6010973773860(K) (H)
1277.81421277.7105814734830(K) (T)
1383.82461383.6909971861970(K) (V)
1475.91851475.74941152122230(K) (T)
1475.91851475.7858902002110(R) (W)
1688.10661687.97078166821(R) (G)
1994.20021993.97721122242390(R) (R)
2150.30302150.07831042242401(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (128/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/126 matches (6%).
Acc. #: Species: MOUSE Name: Kininogen precursor [Contains: Bradykinin]
Index: MW: 73103 Da pI: 6.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
961.4401961.5318-953063150(K) (Y)
1165.56271165.5676-4.12302390(R) (I)
1181.61741181.5625471Met-ox 2302390(R)GNLFMDINNK(I)
1249.70341249.574110365750(K) (Y)
1304.70041304.6738206196300(K) (C)
2288.40862288.2138854554751(R) (L)
2319.31932319.1019941611790(K) (K)
2717.40212717.3319261371600(K) (H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (107/661AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 14/126 matches (11%).
Acc. #: Species: HUMAN Name: Transforming acidic coiled-coil-containing protein 3
Index: MW: 90360 Da pI: 5.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
961.4401961.5471-11155620(K) (D)
977.4609977.4978-388278340(R) (M)
1047.51101047.5509-38190(-) (N)
1125.51381125.6268-10038471(R) (N)
1271.54501271.5690-193884000(R) (G)
1291.85071291.719710152621(K) (D)
1307.81511307.7146771Met-ox 52621(K)AMKVTFQTPLR(D)
1383.82461383.7596477757860(K) (S)
1434.92001434.77051047577681(R) (A)
1475.91851475.826263pyroGlu 5575681(K)QSLYLKFDPLLR(D)
1553.88531553.69461234104230(K) (S)
1852.09811851.90231067497631(K) (Y)
1871.98671871.98023.52382570(R) (E)
3208.87693208.49621195225501(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (158/838AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 9/126 matches (7%).
Acc. #: Species: MOUSE Name: Signal transducer and activator of transcription 4
Index: MW: 85941 Da pI: 6.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1136.52041136.5258-4.71771860(K) (N)
1152.64451152.52071071Met-ox 1771860(K)TIQTMDQGDK(N)
1330.78901330.7735123513611(K) (A)
1548.99791548.8790776937060(K) (S)
1566.04191565.87251081151290(R) (S)
1605.85771605.7073941631740(K) (Y)
1949.21151949.1013572993141(R) (N)
1997.19171997.0788575485630(K) (K)
2379.42912379.1814104941131(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (114/749AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 10/126 matches (7%).
Acc. #: Species: HUMAN Name: Adenylyl cyclase-associated protein 1 (CAP 1)
Index: MW: 51673 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1090.51401090.5931-723563640(K) (G)
1133.54391133.526116AcetN 1Met-ox 1100(-)ADMQNLVER(L)
1165.56271165.5312274134220(K) (N)
1249.70341249.6098754234330(K) (S)
1765.91551765.87682214291(R) (G)
1949.21151948.991511364811(K) (L)
1956.28661956.06281143493641(K) (G)
1994.20021994.0823592953131(R) (A)
2351.38772351.22876838600(K) (I)
2396.33282396.1226884134331(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (123/475AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 8/126 matches (6%).
Acc. #: Species: HUMAN Name: Cell division control protein 2 homolog (P34 protein kinase) (Cyclin-dependent kinase 1) (CDK1)
Index: MW: 34096 Da pI: 8.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
950.3900950.4981-1141Met-ox 25330(K)TTGQVVAMK(K)
950.3900950.5021-1182672740(K) (R)
1330.78901330.6854782552660(K) (M)
1797.12011796.9812772392541(K) (N)
1852.09811851.93878660750(R) (L)
1913.14351912.9366108901060(K) (S)
1998.19351998.0271831071220(K) (R)
2041.26362041.0316114891061(K) (S)
2705.45822705.3575372162381(R) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 39% (118/297AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 8/126 matches (6%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
Index: MW: 59519 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1090.51401090.5315-161481560(K) (L)
1165.56271165.5853-194424500(R) (S)
1434.92001434.77051044404501(K) (S)
1493.89041493.73481043233341(R) (D)
1707.95021707.772710441590(K) (G)
1797.12011797.0122603713860(R) (Q)
1997.19171996.97151103463620(K) (S)
2367.45682367.2632822082280(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (105/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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