MS-Fit Search Results



.

Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D172
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 4152 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score
#/104(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.420e+01224 (23)41.023.166.849.7 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
25.093e+00819 (18)30.018.360.047.5 59519/5.1HUMANKeratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
32.348e+00816 (15)28.015.451.759.4 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
43.449e+00718 (17)33.017.346.468.8 59999/8.1HUMANKeratin, type II cytoskeletal 6B (Cytokeratin 6B) (CK 6B) (K6B keratin)
52.797e+00718 (17)31.017.346.970.6 60045/8.1HUMANKeratin, type II cytoskeletal 6A (Cytokeratin 6A) (CK 6A) (K6A keratin)
62.790e+00718 (17)33.017.346.468.8 60201/8.1HUMANKeratin, type II cytoskeletal 6C (Cytokeratin 6C) (CK 6C) (K6C keratin)
72.253e+00717 (16)42.016.342.661.6 42469/5.3HUMANKeratin, type II cytoskeletal 6D (Cytokeratin 6D) (CK 6D) (K6D keratin)
81.904e+00717 (16)33.016.351.258.6 60067/8.1HUMANKeratin, type II cytoskeletal 6F (Cytokeratin 6F) (CK 6F) (K6F keratin)
91.899e+00717 (16)33.016.351.158.7 60224/8.1HUMANKeratin, type II cytoskeletal 6E (Cytokeratin 6E) (CK 6E) (K6E keratin)
105.399e+00616 (15)24.015.448.559.9 62462/8.1HUMANKeratin, type II cytoskeletal 5 (Cytokeratin 5) (K5) (CK 5) (58 kDa cytokeratin)
46726x...x...x......xx...........x..x........x..x...xx.x....x..x.x.....x....x...xxxx.x.........x.......x.....
111704.xxx.........x.....x...x.....x...x..x.x........x..x....xx......x.....x.........x.................x.....x
100557.............xxx....x...xxxxx....................x....x.............................xxx............xx...
64370....x.x........x.....xx....x.xx...x....x............x.xx.....x..xx.....x...............x................
87004....x.x........x.....xx....x.xx...x....x............x.xx.....x..xx.....x...............x................
52258....x.x........x.....xx....x.xx...x....x............x.xx.....x..xx.....x...............x................
31153....x.x........x.....xx....x.xx...x....x............x.xx.....x...x.....x...............x................
21769......x...x....x.....xx....x...x..x....x............x.xx.....x..xx.....x...............x................
14705......x...x....x.....xx....x.x....x....x............x.xx.....x..xx.....x...............x................
114763....x.x........x....x......x..x.x.x....x....x.......x......x..x.x..x...................x................



Detailed Results

1. 24/104 matches (23%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
973.5596973.5318293964030(K) (V)
1016.56401016.5013623563640(K) (S)
1033.56831033.5166504844920(R) (M)
1066.56321066.5169432702771(K) (T)
1070.59541070.501987pyroGlu 4121(R)QFSSRSGYR(S)
1157.64111157.51491091Met-ox 4644720(R)DYQELMNTK(L)
1179.65881179.6010493773860(K) (H)
1265.70921265.6378562782880(R) (K)
1277.77581277.63371112472571(K) (N)
1277.77581277.7105514734830(K) (T)
1300.68381300.53021182582670(K) (N)
1302.74311302.7018323934031(R) (V)
1302.74311302.7157213443550(R) (A)
1316.65111316.5251961Met-ox 2582670(K)NMQDMVEDYR(N)
1357.78011357.6963624444550(K) (E)
1383.77021383.6909571861970(K) (V)
1393.82751393.7327682782891(R) (D)
1475.85661475.7494732122230(K) (T)
1475.85661475.7858482002110(R) (W)
1523.87791523.7892584614721(R) (L)
1638.97581638.8604701861991(K) (F)
1657.90471657.79346713290(R) (R)
1688.05831687.97075266821(R) (G)
1699.96931699.825185pyroGlu 4184320(K)QISNLQQSISDAEQR(G)
1716.97821716.8517744184320(K) (G)
1994.14891993.9772862242390(R) (R)
2384.16772383.9524905195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 41% (270/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 19/104 matches (18%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
Index: MW: 59519 Da pI: 5.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
993.5533993.5005532382450(K) (Q)
995.5341995.5202141781841(K) (H)
1003.57741003.5536243633701(K) (N)
1060.60181060.47331211Met-ox 2852921(K)KNHEEEMK(D)
1060.60181060.5097871Met-ox 191(-)MSVRYSSSK(H)
1090.57951090.5315441481560(K) (L)
1109.54771109.4904523353430(K) (S)
1165.63891165.5853464424500(R) (S)
1201.68221201.6177542462560(R) (R)
1234.73551234.6796452362451(R) (Q)
1262.68901262.5977724514640(R) (G)
1300.68381300.5956682862951(K) (N)
1316.65111316.5905461Met-ox 2862951(K)NHEEEMKDLR(N)
1357.78011357.7188452462571(R) (V)
1365.72831365.6399653233330(R) (K)
1434.86021434.7705634404501(K) (S)
1493.83331493.7348663233341(R) (D)
1707.90001707.77277541590(K) (G)
2367.43062367.2632712082280(K) (L)
2872.65662872.3933922963220(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (179/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 16/104 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1060.60181060.5639362242320(K) (M)
1065.56401065.4999601541620(K) (L)
1066.56321066.4992602422490(K) (Q)
1095.58371095.5475331841911(K) (G)
1121.57481121.5843-8.43273350(R) (N)
1138.60751138.5931132332411(R) (F)
1140.61611140.564945pyroGlu 2502600(R)QGVDADINGLR(Q)
1154.60791154.5880171Met-ox 2332411(R)MTLDDFRIK(F)
1157.64111157.5915432502600(R) (Q)
1307.74851307.6782542402491(R) (Q)
1350.76451350.6878574724861(K) (G)
1791.89491791.7283934905120(R) (G)
1838.07821837.9660613743890(R) (K)
1852.07131851.9275783213351(K) (N)
2510.35242510.1323884494710(K) (I)
2705.42952705.16179963940(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 28% (178/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 18/104 matches (17%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 6B (Cytokeratin 6B) (CK 6B) (K6B keratin)
Index: MW: 59999 Da pI: 8.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1016.56401016.5376262242320(R) (G)
1026.56051026.45661012732810(K) (V)
1026.56051026.52203831400(R) (S)
1066.56321066.5169432532601(K) (T)
1096.59011096.6115-193913991(R) (K)
1107.59751107.5435493393470(K) (S)
1154.60791154.5516492722811(K) (V)
1165.63891165.5853463603690(K) (H)
1172.60681172.6388-272232321(R) (G)
1203.68861203.6374431952040(K) (T)
1263.73281263.6949304564660(K) (K)
1329.78541329.7266443273380(R) (A)
1350.76451350.6766653393491(K) (A)
1350.76451350.7269282612721(R) (D)
1357.78011357.7327354254361(K) (A)
1407.79461407.7120592882990(K) (A)
1438.82791438.649712443590(R) (S)
1455.79661455.6868753483591(R) (Y)
1523.87791523.8143421Met-ox 4444551(R)LLKEYQELMNVK(L)
1891.12111890.9714792082220(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 33% (190/564AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 18/104 matches (17%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 6A (Cytokeratin 6A) (CK 6A) (K6A keratin)
Index: MW: 60045 Da pI: 8.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1016.56401016.5376262242320(R) (G)
1026.56051026.45661012732810(K) (V)
1066.56321066.5169432532601(K) (T)
1096.59011096.6115-193913991(R) (K)
1107.59751107.5435493393470(K) (S)
1154.60791154.5516492722811(K) (V)
1165.63891165.5853463603690(K) (H)
1172.60681172.6388-272232321(R) (G)
1203.68861203.6374431952040(K) (T)
1263.73281263.6949304564660(K) (K)
1329.78541329.7266443273380(R) (A)
1350.76451350.6766653393491(K) (A)
1350.76451350.7269282612721(R) (D)
1357.78011357.7327354254361(K) (A)
1407.79461407.7120592882990(K) (A)
1438.82791438.649712443590(R) (S)
1455.79661455.6868753483591(R) (Y)
1523.87791523.8143421Met-ox 4444551(R)LLKEYQELMNVK(L)
1891.12111890.9714792082220(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (180/564AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 18/104 matches (17%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 6C (Cytokeratin 6C) (CK 6C) (K6C keratin)
Index: MW: 60201 Da pI: 8.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1016.56401016.5376262242320(R) (G)
1026.56051026.45661012732810(K) (V)
1026.56051026.52203831400(R) (S)
1066.56321066.5169432532601(K) (T)
1096.59011096.6115-193913991(R) (K)
1107.59751107.5435493393470(K) (S)
1154.60791154.5516492722811(K) (V)
1165.63891165.5853463603690(K) (H)
1172.60681172.6388-272232321(R) (G)
1203.68861203.6374431952040(K) (T)
1263.73281263.6949304564660(K) (K)
1329.78541329.7266443273380(R) (A)
1350.76451350.6766653393491(K) (A)
1350.76451350.7269282612721(R) (D)
1357.78011357.7327354254361(K) (A)
1407.79461407.7120592882990(K) (A)
1438.82791438.649712443590(R) (S)
1455.79661455.6868753483591(R) (Y)
1523.87791523.8143421Met-ox 4444551(R)LLKEYQELMNVK(L)
1891.12111890.9714792082220(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 33% (190/564AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 17/104 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 6D (Cytokeratin 6D) (CK 6D) (K6D keratin)
Index: MW: 42469 Da pI: 5.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1016.56401016.53762644520(R) (G)
1026.56051026.4566101931010(K) (V)
1066.56321066.51694373801(K) (T)
1096.59011096.6115-192112191(R) (K)
1107.59751107.5435491591670(K) (S)
1154.60791154.551649921011(K) (V)
1165.63891165.5853461801890(K) (H)
1172.60681172.6388-2743521(R) (G)
1203.68861203.63744315240(K) (T)
1263.73281263.6949302762860(K) (K)
1329.78541329.7266441471580(R) (A)
1350.76451350.6766651591691(K) (A)
1350.76451350.72692881921(R) (D)
1357.78011357.7327352452561(K) (A)
1407.79461407.7120591081190(K) (A)
1455.79661455.6868751681791(R) (Y)
1523.87791523.8143421Met-ox 2642751(R)LLKEYQELMNVK(L)
1891.12111890.97147928420(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 42% (163/384AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 17/104 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 6F (Cytokeratin 6F) (CK 6F) (K6F keratin)
Index: MW: 60067 Da pI: 8.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1026.56051026.45661012732810(K) (V)
1026.56051026.522038pyroGlu 2242320(R)QLDNIVGER(G)
1026.56051026.52203831400(R) (S)
1043.60511043.5485542242320(R) (G)
1066.56321066.5169432532601(K) (T)
1096.59011096.6115-193913991(R) (K)
1107.59751107.5435493393470(K) (S)
1154.60791154.5516492722811(K) (V)
1179.65881179.6010493603690(K) (H)
1203.68861203.6374431952040(K) (T)
1263.73281263.6949304564660(K) (K)
1329.78541329.7266443273380(R) (A)
1350.76451350.6766653393491(K) (A)
1350.76451350.7269282612721(R) (D)
1357.78011357.7327354254361(K) (A)
1407.79461407.7120592882990(K) (A)
1438.82791438.649712443590(R) (S)
1455.79661455.6868753483591(R) (Y)
1523.87791523.8143421Met-ox 4444551(R)LLKEYQELMNVK(L)
1891.12111890.9714792082220(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 33% (189/564AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 17/104 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 6E (Cytokeratin 6E) (CK 6E) (K6E keratin)
Index: MW: 60224 Da pI: 8.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1026.56051026.45661012732810(K) (V)
1026.56051026.522038pyroGlu 2242320(R)QLDNIVGER(G)
1026.56051026.52203831400(R) (S)
1043.60511043.5485542242320(R) (G)
1066.56321066.5169432532601(K) (T)
1096.59011096.6115-193913991(R) (K)
1107.59751107.5435493393470(K) (S)
1154.60791154.5516492722811(K) (V)
1165.63891165.5853463603690(K) (H)
1203.68861203.6374431952040(K) (T)
1263.73281263.6949304564660(K) (K)
1329.78541329.7266443273380(R) (A)
1350.76451350.6766653393491(K) (A)
1350.76451350.7269282612721(R) (D)
1357.78011357.7327354254361(K) (A)
1407.79461407.7120592882990(K) (A)
1438.82791438.649712443590(R) (S)
1455.79661455.6868753483591(R) (Y)
1523.87791523.8143421Met-ox 4444551(R)LLKEYQELMNVK(L)
1891.12111890.9714792082220(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 33% (189/564AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 16/104 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 5 (Cytokeratin 5) (K5) (CK 5) (58 kDa cytokeratin)
Index: MW: 62462 Da pI: 8.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1016.56401016.5376262292370(R) (G)
1026.56051026.45661012782860(K) (V)
1066.56321066.5169432582651(K) (T)
1095.58371095.554726AcetN 1101(-)SRQSSVSFR(S)
1154.60791154.5516492772861(K) (V)
1172.60681172.6388-272282371(R) (G)
1194.64001194.5755543653740(K) (H)
1203.68861203.6374432002090(K) (T)
1263.73281263.6949304614710(K) (K)
1282.71721282.6353642662760(R) (K)
1329.78541329.7266443323430(R) (A)
1385.82901385.7640474304411(R) (A)
1424.75201424.69564049630(R) (S)
1438.82791438.7364642652761(K) (K)
1485.83631485.6974933533641(R) (Y)
1891.12111890.9714792132270(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (146/590AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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