MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D172
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 2641 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/83(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.410e+01321 (25)41.025.364.826.8 56651/6.3HUMAND-3-phosphoglycerate dehydrogenase (3-PGDH)
21.306e+00611 (13)26.013.354.074.4 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
32.649e+0059 (10)32.010.812.6115 29572/8.4HUMANExosome complex exonuclease RRP40 (Ribosomal RNA processing protein 40) (p10) (CGI-102)
41.896e+0058 (9)17.09.642.2134 51449/6.5MOUSED-3-phosphoglycerate dehydrogenase (3-PGDH) (A10)
51.776e+00510 (12)20.012.058.6105 55246/8.2MOUSECASP8 and FADD-like apoptosis regulator precursor (Cellular FLICE-like inhibitory protein) (c-FLIP) (Caspase-eight-related protein) (Casper) (Caspase-like apoptosis regulatory protein) (CLARP) (MACH-related inducer of toxicity) (MRIT) (Caspase homolog)>
65.578e+00411 (13)25.013.350.476.5 53675/5.5HUMANKeratin, type II cytoskeletal 8 (Cytokeratin 8) (K8) (CK 8)
73.865e+0049 (10)19.010.859.180.2 72038/5.8MOUSEINTERFERON-INDUCED GTP-BINDING PROTEIN MX1 (INFLUENZA RESISTANCE PROTEIN)
83.835e+00410 (12)14.012.051.782.1 96697/6.4HUMANGlycogen phosphorylase, brain form
93.596e+0048 (9)25.09.663.767.5 40871/9.4HUMANRNA 3'-terminal phosphate cyclase-like protein (HSPC338)
103.517e+00410 (12)19.012.066.490.4 78440/6.9HUMANPR-domain zinc finger protein 17
37755............x....x.......x......x..x..x..x...x.x...x.xxx.......x....x...x..x.xx..xx
46726....x..........x......x.x............x........x......x...x.x.......x............x..
38321...xx.........x.................................x............x.....x....x..x.x.....
87323...........xx....x...................................x.x............x...x.........x
36069.......x........x.x......xx.....x.......x.....................x...............xx...
28336....x.x..x......x.x.....x....x..........x.....x..............................xx....
91413....x......................x..x........x.x....xx.......................xx..........
43800......x............x...............xx.x.x...x....x......x..................x.......
22600..x....xx.............................................x....x.....x....xx...........
72891.....x.....................x.........xx......x.x.x............x..x....x............


Detailed Results

1. 21/83 matches (25%).
Acc. #: Species: HUMAN Name: D-3-phosphoglycerate dehydrogenase (3-PGDH)
Index: MW: 56651 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1099.69041099.6111722372470(R) (A)
1130.63591130.60572759690(K) (L)
1298.80841298.73205922330(K) (Q)
1345.85091345.7691613523640(K) (N)
1373.73691373.627979pyroGlu 5235330(K)QHVTEAFQFHF(-)
1373.73691373.673546pyroGlu 1Met-ox 1201321(R)QIPQATASMKDGK(W)
1390.75471390.6544725235330(K) (-)
1390.75471390.7000391Met-ox 1201321(R)QIPQATASMKDGK(W)
1426.92001426.82706521331(R) (Q)
1462.81281462.7034759200(K) (K)
1488.84991488.72948176900(R) (K)
1590.90621590.7984688201(R) (K)
1590.90621590.7984689211(K) (I)
1638.91051638.7871752953080(R) (G)
1640.01021639.9205553653800(K) (E)
1654.90261654.7821731Met-ox 2953080(R)CGEEIAVQFVDMVK(G)
1901.11121900.96907539540(K) (S)
2062.19682062.0391762712890(R) (E)
2231.21472231.0767622482680(R) (D)
2272.32452272.1681694704910(R) (L)
2288.29222288.1630561Met-ox 4704910(R)TQTSDPAMLPTMIGLLAEAGVR(L)
2288.29222288.18084939581(K) (V)
2304.34092304.1579792Met-ox 4704910(R)TQTSDPAMLPTMIGLLAEAGVR(L)
2471.44742471.28166734541(K) (S)
2502.38232502.2047712482701(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 41% (219/533AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 11/83 matches (13%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1033.56991033.5166524844920(R) (M)
1127.49721127.5407-392892981(K) (V)
1179.67351179.6010613773860(K) (H)
1277.79191277.63371242472571(K) (N)
1277.79191277.7105644734830(K) (T)
1383.76151383.6909511861970(K) (V)
1475.86551475.7494792122230(K) (T)
1475.86551475.7858542002110(R) (W)
1638.91051638.8604311861991(K) (F)
1688.08711687.97076966821(R) (G)
1716.96901716.8517684184320(K) (G)
1994.01611993.9772202242390(R) (R)
2384.11542383.9524685195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (168/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/83 matches (10%).
Acc. #: Species: HUMAN Name: Exosome complex exonuclease RRP40 (Ribosomal RNA processing protein 40) (p10) (CGI-102)
Index: MW: 29572 Da pI: 8.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1027.50931027.5835-7265731(R) (C)
1033.56991033.531837pyroGlu 2672751(K)QIFSRLAES(-)
1109.54381109.6319-791141240(K) (S)
1519.88571519.7943601872010(R) (V)
1838.01181837.835796901070(K) (R)
1994.01611993.936840901081(K) (Y)
2231.21472231.19379.41531720(R) (D)
2272.32452272.2851171872081(R) (K)
2288.29222288.28005.31Met-ox 1872081(R)ANGMGVIGQDGLLFKVTLGLIR(K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (90/275AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/83 matches (9%).
Acc. #: Species: MOUSE Name: D-3-phosphoglycerate dehydrogenase (3-PGDH) (A10)
Index: MW: 51449 Da pI: 6.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1085.52941085.6584-1194134211(R) (A)
1099.69041099.6111721891990(R) (A)
1130.63591130.60572711210(K) (L)
1638.91051638.7871752472600(R) (G)
1654.90261654.7821731Met-ox 2472600(R)CGEEIAVQFVDMVK(G)
2062.19682062.0391762232410(R) (E)
2231.21472231.0767622002200(R) (D)
2502.38232502.2047712002221(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (87/485AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/83 matches (12%).
Acc. #: Species: MOUSE Name: CASP8 and FADD-like apoptosis regulator precursor (Cellular FLICE-like inhibitory protein) (c-FLIP) (Caspase-eight-related protein) (Casper) (Caspase-like apoptosis regulatory protein) (CLARP) (MACH-related inducer of toxicity) (MRIT) (Caspase homolog)>
Index: MW: 55246 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1047.53001047.5322-2.144520(R) (G)
1129.65501129.52081191Met-ox 24310(K)EMMLFLCR(D)
1153.56561153.6329-583863950(K) (H)
1298.80841298.670510632430(R) (D)
1305.79281305.7306481381480(K) (L)
1345.85091345.7592684674771(K) (K)
1412.73811412.6302763433540(K) (G)
1839.04861838.8893871Met-ox 3433581(K)NMFTGDTCPSLRGKPK(L)
2304.34092304.1766712512700(R) (Y)
2320.33762320.1715721Met-ox 2512700(R)MQSKPLGICLIIDCIGNDTK(Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (97/484AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 11/83 matches (13%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 8 (Cytokeratin 8) (K8) (CK 8)
Index: MW: 53675 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1033.56991033.5213471Met-ox 3653721(K)QDMARQLR(E)
1033.56991033.6006-304554641(R) (K)
1045.63031045.5530741501580(R) (L)
1060.62951060.5639623934011(R) (L)
1129.65501129.6217293423521(R) (L)
1129.65501129.6217293533620(K) (A)
1153.56561153.55638.13733810(R) (L)
1277.79191277.7105643823920(K) (K)
1320.72561320.6721412532640(R) (A)
1412.73811412.6844382742851(R) (Y)
1475.86551475.69871134024140(R) (T)
2288.29222288.1120792762951(R) (H)
2304.34092304.10701021Met-ox 2762951(R)AEAESMYQIKYEELQSLAGK(H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (124/483AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 9/83 matches (10%).
Acc. #: Species: MOUSE Name: INTERFERON-INDUCED GTP-BINDING PROTEIN MX1 (INFLUENZA RESISTANCE PROTEIN)
Index: MW: 72038 Da pI: 5.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1033.56991033.473793AcetN 190(-)DSVNNLCR(H)
1307.76431307.6703725845940(K) (C)
1323.75871323.6652711Met-ox 5845940(K)MMLQLLQDTSK(C)
1407.75711407.6504763263371(K) (M)
1426.92001426.8270652082200(R) (G)
1475.86551475.7858541121260(K) (L)
1475.86551475.7892521Met-ox 4284401(R)VKALEESAVNMLR(R)
1488.84991488.9194-475645750(R) (T)
2225.28272225.0667973733891(K) (K)
2231.21472231.0840594534711(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (121/631AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 10/83 matches (12%).
Acc. #: Species: HUMAN Name: Glycogen phosphorylase, brain form
Index: MW: 96697 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1045.63031045.51661097167240(R) (K)
1156.68831156.63264818290(R) (K)
1373.73691373.6887355585690(K) (R)
1380.75271380.6516737617710(K) (F)
1390.75471390.7007393603701(K) (K)
1412.73811412.6415682Met-ox 7617710(K)DIVNMLMHHDR(F)
1412.73811412.8088-506106221(K) (L)
1458.83421458.7844343533641(R) (A)
1534.86031534.7800522362481(K) (A)
1661.98621661.8400884114250(R) (L)
2272.32452272.1614728248431(R) (-)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (123/843AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 8/83 matches (9%).
Acc. #: Species: HUMAN Name: RNA 3'-terminal phosphate cyclase-like protein (HSPC338)
Index: MW: 40871 Da pI: 9.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1013.56531013.51285237451(R) (D)
1047.53001047.5337-3.53333400(K) (I)
1052.61681052.5675471911991(R) (V)
1640.01021639.8552953563700(K) (T)
1716.96901716.8202871581740(R) (K)
1941.07601940.9111851170(-) (Q)
2211.27072211.1524532142311(R) (G)
2225.28272225.0708951191(-) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (95/373AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 10/83 matches (12%).
Acc. #: Species: HUMAN Name: PR-domain zinc finger protein 17
Index: MW: 78440 Da pI: 6.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1036.57351036.6155-415875951(R) (R)
1307.76431307.61661133093190(K) (A)
1383.76151383.7345201361481(R) (I)
1390.75471390.6776551120(-) (K)
1462.81281462.7277586726840(R) (C)
1488.84991488.7092954054160(R) (A)
1534.86031534.7674601Met-ox 1131(-)MEEAEELLLEGKK(A)
1839.04861838.8682985265400(R) (R)
1941.07601940.9145831Met-ox 6096241(R)CPTCGMGYTLPQSLRR(H)
2211.27072211.01881144264440(K) (A)
2211.27072211.1068744895061(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (139/720AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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