MS-Fit Search Results



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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D182
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 739 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score
#/64(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.755e+00711 (17)29.017.248.455.3 53166/5.8HUMANTryptophanyl-tRNA synthetase (Tryptophan--tRNA ligase) (TRPRS) (IFP53) (hWRS)
26.621e+00510 (15)19.015.657.630.7 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
31.820e+0046 (9)11.09.460.266.5 89615/8.6HUMANTumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
41.585e+0048 (12)16.012.514.2111 97287/6.7MOUSEGlycogen phosphorylase, muscle form (Myophosphorylase)
51.124e+0045 (7)16.07.833.789.3 56813/5.5HUMANAlkaline phosphatase, intestinal precursor (IAP)
686287 (10)12.010.948.7102 96866/5.3HUMANHeat-shock protein 105 kDa (Heat shock 110 kDa protein) (Antigen NY-CO-25)
7733710 (15)18.015.667.346.3 79308/5.6HUMANDEAD-box protein 4 (VASA homolog)
849766 (9)35.09.430.497.1 26887/10.9HUMANINT-2 proto-oncogene protein precursor (Fibroblast growth factor-3) (FGF-3) (HBGF-3)
945616 (9)13.09.437.486.2 84727/7.3MOUSEComplement C2 precursor (C3/C5 convertase)
1043677 (10)13.010.9-10.3116 68021/8.2HUMANProtein-tyrosine phosphatase, non-receptor type 9 (Protein-tyrosine phosphatase MEG2) (PTPase-MEG2)
17234...x......x.x.x.......x.............x.....x....x...x..x..x......
45313.x..x..xx..x..x...x...............x....x...........x............
42717....x................x....x.......x...x.......x.................
18650............xxx.....x..........x..x...........x..x..............
3292...x......x.........x......................x.................x..
27666x.............x.x...x....................x......x......x........
50146...........x.xx...x.....................x....x.......xx.xx......
71588.......x.x.....x.......x........x............................x..
49131............................x......x....x..........x.....xx.....
21050.....xx...x.........x...............x.............x....x........



Detailed Results

1. 11/64 matches (17%).
Acc. #: Species: HUMAN Name: Tryptophanyl-tRNA synthetase (Tryptophan--tRNA ligase) (TRPRS) (IFP53) (hWRS)
Index: MW: 53166 Da pI: 5.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1144.65781144.6155371541620(K) (G)
1319.77871319.7085531231330(R) (R)
1430.74701430.6626592652770(K) (I)
1475.85421475.8096301231341(R) (G)
1801.14971801.0336644334480(K) (R)
2084.15562084.0639441Met-ox 1631810(R)GPSSEAMHVGHLIPFIFTK(W)
2253.23882253.0684763013180(R) (M)
2325.36272325.2032692782980(K) (D)
2384.11762384.1709-222572771(K) (I)
2485.48262485.3607493273490(R) (M)
2524.38142524.1965732993181(R) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 29% (141/471AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/64 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1070.52111070.501918pyroGlu 4121(R)QFSSRSGYR(S)
1179.67591179.6010643773860(K) (H)
1277.77661277.7105524734830(K) (T)
1300.61711300.5302672582670(K) (N)
1383.76441383.6909531861970(K) (V)
1475.85421475.7494712122230(K) (T)
1475.85421475.7858462002110(R) (W)
1638.96461638.8604641861991(K) (F)
1994.10301993.9772632242390(R) (R)
2150.22402150.0783682242401(R) (R)
2384.11762383.9524695195490(R) (G)

Click link below to search for cysteine linked fragments.

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Click link below to search for another component.

The matched peptides cover 19% (126/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/64 matches (9%).
Acc. #: Species: HUMAN Name: Tumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
Index: MW: 89615 Da pI: 8.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1179.67591179.55811006776850(K) (F)
1791.92731791.8601386776901(K) (R)
1852.02601851.9751274214390(K) (G)
1994.10301994.05002725410(R) (T)
2148.14842147.9755804804970(R) (C)
2307.31662307.1113893663860(R) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (89/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/64 matches (12%).
Acc. #: Species: MOUSE Name: Glycogen phosphorylase, muscle form (Myophosphorylase)
Index: MW: 97287 Da pI: 6.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1430.74701430.7652-136106221(K) (L)
1442.81111442.6956802792900(R) (E)
1475.85421475.7535683603711(R) (T)
1765.84781765.9489-572963100(K) (R)
1765.84781765.9713-705565701(K) (I)
1941.08111941.0856-2.45765901(K) (I)
1994.10301994.02483945611(R) (D)
2307.31662307.1484732162350(K) (N)
2347.30382347.28089.86196401(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (137/842AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/64 matches (7%).
Acc. #: Species: HUMAN Name: Alkaline phosphatase, intestinal precursor (IAP)
Index: MW: 56813 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1144.65781144.5791693243330(R) (I)
1319.77871319.7072541241360(K) (F)
1765.84781765.8795-181071230(R) (A)
2283.34772283.17747514330(R) (Q)
2717.29422717.3238-111992230(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (85/528AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 7/64 matches (10%).
Acc. #: Species: HUMAN Name: Heat-shock protein 105 kDa (Heat shock 110 kDa protein) (Antigen NY-CO-25)
Index: MW: 96866 Da pI: 5.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1028.51081028.5376-267737810(K) (A)
1475.85421475.7342813613740(K) (G)
1620.91401620.7593956566670(K) (L)
1765.84781765.8696-121Met-ox 7157281(K)MFEELGQRLQHYAK(I)
2223.20192223.0361757547721(K) (S)
2343.14402343.0671332762950(K) (D)
2497.44182497.2040954374581(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (110/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 10/64 matches (15%).
Acc. #: Species: HUMAN Name: DEAD-box protein 4 (VASA homolog)
Index: MW: 79308 Da pI: 5.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1383.76441383.6475854514621(R) (L)
1442.81111442.6698981Met-ox 76891(K)RDNTSTMGGFGVGK(S)
1475.85421475.8222223763871(R) (F)
1638.96461638.8161915375501(R) (K)
2211.26062211.0868794734901(K) (L)
2298.33842298.1739722Met-ox 3393590(K)TAAFLLPILAHMMHDGITASR(F)
2468.43612468.282862pyroGlu 4114321(R)QIVQGCNILCATPGRLMDIIGK(E)
2485.48262485.3093704114321(R) (E)
2501.41452501.3042441Met-ox 4114321(R)QIVQGCNILCATPGRLMDIIGK(E)
2524.38142524.2506525535741(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (135/724AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 6/64 matches (9%).
Acc. #: Species: HUMAN Name: INT-2 proto-oncogene protein precursor (Fibroblast growth factor-3) (FGF-3) (HBGF-3)
Index: MW: 26887 Da pI: 10.9
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1277.77661277.7330341932040(R) (G)
1307.75851307.7072391331441(R) (R)
1531.93871531.8498581521641(R) (R)
1815.15741815.0353671932091(R) (R)
1958.10351958.00714948631(K) (V)
2717.29422717.4725-6664890(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (84/239AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 6/64 matches (9%).
Acc. #: Species: MOUSE Name: Complement C2 precursor (C3/C5 convertase)
Index: MW: 84727 Da pI: 7.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1927.15141926.9959813763931(R) (K)
1998.11221998.0700215415580(K) (R)
2211.26062211.2065242913100(R) (T)
2384.11762384.1823-275826020(K) (R)
2524.38142524.2935354014221(R) (L)
2556.50952556.2784901Met-ox 5826031(K)MSTHARPICLPCTVGANMALRR(S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (100/760AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 7/64 matches (10%).
Acc. #: Species: HUMAN Name: Protein-tyrosine phosphatase, non-receptor type 9 (Protein-tyrosine phosphatase MEG2) (PTPase-MEG2)
Index: MW: 68021 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1194.53321194.5618-244124200(K) (D)
1245.66681245.7822-931972070(R) (D)
1319.77871319.6959631031150(R) (L)
1765.84781765.9390-5256691(K) (E)
2084.15562084.0275614264430(R) (K)
2369.22642369.20767.91Met-ox 4244431(R)IRFGFLTVTNLGVENMNHYK(K)
2497.44182497.5314-361862071(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (78/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


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