MS-Fit Search Results



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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D183
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1894 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/88(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.726e+00614 (15)32.015.9-73.936.1 54168/6.1HUMANMitochondrial processing peptidase beta subunit, mitochondrial precursor (Beta-MPP) (P-52)
22.867e+00510 (11)23.011.4-30.292.5 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
39.959e+00413 (14)26.014.8-37.979.2 69873/7.8HUMANTyrosine-protein kinase ZAP-70 (70 kDa zeta-associated protein) (Syk-related tyrosine kinase)
43.868e+00413 (14)21.014.8-19.589.6 74140/6.6HUMANLamin A/C (70 kDa lamin)
52.684e+0048 (9)12.09.1-23.399.6 69501/8.2HUMANBifunctional 3'-phosphoadenosine 5'-phosphosulfate synthethase 2 (PAPS synthethase 2) (PAPSS 2) (Sulfurylase kinase 2) (SK2) (SK 2) [Includes: Sulfate adenylyltransferase (Sulfate adenylate transferase) (SAT) (ATP-sulfurylase); Adenylylsulfate kinase (>
109265.x.....xxxxx.......x.............x......x............x....x.x.......x......x............
46726...............x.x.......x.....x...........x.......x..x.....x...............x.......x...
40982.x............x...........x......x.x.........x......x............xx.x.......x...x.x.....
78186..x....x.........x................x.....x..x...xx.x..xx.x.................x.............
83345........x.x......x.........x.x...............x...........................x...x..........



Detailed Results

1. 14/88 matches (15%).
Acc. #: Species: HUMAN Name: Mitochondrial processing peptidase beta subunit, mitochondrial precursor (Beta-MPP) (P-52)
Index: MW: 54168 Da pI: 6.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1014.37491014.4501-741Met-ox 4254310(R)QMLCYNR(R)
1048.46161048.5461-8160680(R) (V)
1085.53031085.6206-832102190(R) (S)
1095.39921095.4716-664824891(R) (-)
1101.48841101.5904-934424510(R) (E)
1111.39221111.4665-671Met-ox 4824891(R)SNMCWLRD(-)
1193.60621193.7006-794324411(R) (I)
1365.59991365.6684-503883990(R) (A)
1434.63211434.7269-661371481(R) (D)
1434.63211434.7745-9916280(R) (G)
1677.75911677.8600-602252381(K) (I)
1713.81341713.9135-5844590(R) (V)
1751.83221752.0060-994624781(R) (Q)
1891.97551892.0493-392392570(R) (F)
1982.91301983.0987-9442591(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (159/489AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/88 matches (11%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1143.45961143.5356-661Met-ox 2892981(K)KDVDGAYMTK(V)
1179.55991179.6010-353773860(K) (H)
1300.53311300.53022.22582670(K) (N)
1332.62431332.5200782Met-ox 2582670(K)NMQDMVEDYR(N)
1475.68861475.7494-412122230(K) (T)
1475.68861475.7858-662002110(R) (W)
1638.72271638.8604-841861991(K) (F)
1687.84991687.9707-7266821(R) (G)
1751.83221751.82722.86036241(R) (S)
1993.94171993.9772-182242390(R) (R)
2383.87192383.9524-345195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (154/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 13/88 matches (14%).
Acc. #: Species: HUMAN Name: Tyrosine-protein kinase ZAP-70 (70 kDa zeta-associated protein) (Syk-related tyrosine kinase)
Index: MW: 69873 Da pI: 7.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1014.37491014.3872-122Met-ox 3783850(K)ADTEEMMR(E)
1140.48271140.5472-561Met-ox 1201281(R)DAMVRDYVR(Q)
1302.55331302.5966-334854960(K) (S)
1365.59991365.7167-854734841(R) (A)
1379.51851379.6452-921Met-ox 5936031(R)MRACYYSLASK(V)
1497.62781497.7313-695055151(K) (F)
1648.77521648.7819-4.02842980(R) (I)
1804.83471804.8830-272832981(R) (I)
1826.79141826.9223-723864000(R) (L)
1891.97551891.8761531611751(R) (K)
1993.94171993.9554-6.91Met-ox 5805941(K)WEDRPDFLTVEQRMR(A)
2211.02612211.1153-404314500(R) (Y)
2233.03572233.1399-473343531(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (162/619AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 13/88 matches (14%).
Acc. #: Species: HUMAN Name: Lamin A/C (70 kDa lamin)
Index: MW: 74140 Da pI: 6.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1025.51351025.4508612Met-ox 3443511(R)EMAEMRAR(M)
1048.46161048.5097-461Met-ox 1821890(K)QLQDEMLR(R)
1179.55991179.5138396556640(R) (-)
1374.57361374.6290-402502600(R) (K)
1434.63211434.6721-285855970(R) (A)
1475.68861475.7681-541Met-ox 3673781(K)LALDMEIHAYRK(L)
1536.76621536.6753594584700(K) (R)
1566.65111566.7512-646286440(R) (S)
1604.74111604.7768-224364501(R) (F)
1677.75911677.8052-275835971(R) (A)
1687.84991687.8727-149251(R) (I)
1708.69191708.7713-461Met-ox 4584711(K)SNEDQSMGNWQIKR(Q)
1961.85211961.9932-7233481(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (143/664AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/88 matches (9%).
Acc. #: Species: HUMAN Name: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthethase 2 (PAPS synthethase 2) (PAPSS 2) (Sulfurylase kinase 2) (SK2) (SK 2) [Includes: Sulfate adenylyltransferase (Sulfate adenylate transferase) (SAT) (ATP-sulfurylase); Adenylylsulfate kinase (>
Index: MW: 69501 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1085.53031085.4726535615690(K) (H)
1101.48841101.4675191Met-ox 5615690(K)AMDFYDPAR(H)
1179.55991179.5468115135230(R) (K)
1307.57151307.6418-545135241(R) (D)
1314.64001314.6846-346006091(K) (S)
1497.62781497.7562-8615270(K) (N)
1940.86171940.8878-131741900(K) (V)
2082.95112083.1228-822522690(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (79/614AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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