MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D184
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 1674 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/96(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.247e+00822 (22)41.022.920.235.9 62640/6.4HUMANStress-induced-phosphoprotein 1 (STI1) (Hsp70/Hsp90-organizing protein) (Transformation-sensitive protein IEF SSP 3521)
21.714e+00718 (18)30.018.816.136.0 74210/6.5MOUSELamin A
31.705e+00719 (19)34.019.817.839.7 74140/6.6HUMANLamin A/C (70 kDa lamin)
41.247e+00712 (12)27.012.57.7929.2 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
55.003e+00617 (17)32.017.715.837.0 65447/6.4MOUSELamins C and C2
69.789e+0059 (9)23.09.49.3041.0 64466/6.4HUMANBifunctional purine biosynthesis protein PURH [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR transformylase); IMP cyclohydrolase (Inosinicase) (IMP synthetase) (ATIC)]
72.772e+00511 (11)14.011.5-0.52552.1 97657/5.5HUMANSplicing factor 3B subunit 2 (Spliceosome associated protein 145) (SAP 145) (SF3b150) (Pre-mRNA splicing factor SF3b 145 kDa subunit)
81.682e+0059 (9)14.09.42.7962.3 80509/5.5HUMANAdseverin (Scinderin)
95.129e+00410 (10)15.010.414.338.6 83935/6.4HUMANDystrobrevin alpha (Dystrobrevin-alpha)
104.118e+0049 (9)14.09.414.563.1 95203/8.9MOUSEGlutamate receptor, ionotropic kainate 1 precursor (Glutamate receptor 5) (GluR-5) (GluR5)
52876xx..x.x...xxx..xx.x.....x....x.......x..x..x.......x...............xx...x.x.x....x..............
14097..xx..xxx.x..x...x....x..........x.......x......x.x..x....xx...x.........x......................
75781..xx..xxx.x..x...x....x....x.....x...x...x......x.x..x....x....x.........x......................
45313.x.......x...........x......x..x......x..x.............xx......................x...x......x.....
109550..xx..xxx.x..x...x....x..........x.......x........x..x....xx...x.........x......................
83809.....x.............x..............x.....x...........x........x..................x..x........x...
12970.x......x...............xxx.....x.x..................x.........x.............x.x................
10776x.......x.............x.....x...............x........................x..x......x..x.............
3064........x.................x..x..............x......x.....x.x..............x......x..x...........
4713.........x...................x...........x...x.x...............x......xx.....x..................


Detailed Results

1. 22/96 matches (22%).
Acc. #: Species: HUMAN Name: Stress-induced-phosphoprotein 1 (STI1) (Hsp70/Hsp90-organizing protein) (Transformation-sensitive protein IEF SSP 3521)
Index: MW: 62640 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
900.5498900.5155381541600(R) (N)
909.4435909.4066414634700(K) (C)
909.4435909.4140323743810(K) (H)
909.4435909.4318132302370(K) (K)
1004.58661004.55293479870(K) (F)
1018.58221018.5607211Met-ox 5065130(R)LILEQMQK(D)
1043.56691043.55986.82852931(K) (E)
1065.58261065.5580231451530(R) (E)
1074.61541074.6563-384074150(K) (D)
1100.63571100.6502-135345430(K) (-)
1116.67701116.6451291Met-ox 5345430(K)LMDVGLIAIR(-)
1132.67901132.64792878871(R) (F)
1214.62911214.6057193063151(R) (Y)
1298.69781298.6533343163251(K) (S)
1377.68321377.6360341Met-ox 4434531(K)DYTKAMDVYQK(A)
1464.77531464.73482833440(K) (S)
1488.81941488.7950163523640(R) (N)
1608.89291608.83863479921(K) (R)
1803.85931803.85621.72Met-ox 1231361(R)KFMNPFNMPNLYQK(L)
1809.85331809.84037.24164290(K) (G)
1870.96431870.929918941091(R) (E)
1921.91251921.8458352Met-ox 4895050(R)AMADPEVQQIMSDPAMR(L)
1937.89701937.8407293Met-ox 4895050(R)AMADPEVQQIMSDPAMR(L)
2081.05152081.02251414320(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 41% (227/543AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 18/96 matches (18%).
Acc. #: Species: MOUSE Name: Lamin A
Index: MW: 74210 Da pI: 6.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
972.5498972.5114393893970(R) (S)
974.5172974.4795393793860(K) (L)
1018.58221018.55332891991(K) (A)
1023.54531023.5111332092160(K) (E)
1028.60181028.5740272412490(R) (A)
1043.56691043.5485181241330(K) (L)
1089.58651089.55403051600(R) (L)
1119.55951119.5897-271451550(K) (R)
1182.64911182.6119321571660(R) (G)
1320.63011320.6409-8.24284391(R) (V)
1475.78661475.7681131Met-ox 3673781(K)LALDMEIHAYRK(L)
1566.78471566.7512216296450(R) (S)
1605.83821605.8124164404531(R) (L)
1629.82681629.80841129411(R) (L)
1699.96341699.9707-4.32973111(R) (Q)
1707.79331707.8050-6.94214351(K) (T)
1752.88701752.8629142812960(R) (I)
1909.95121909.9217151Met-ox 3523660(R)MQQQLDEYQELLDIK(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (205/665AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 19/96 matches (19%).
Acc. #: Species: HUMAN Name: Lamin A/C (70 kDa lamin)
Index: MW: 74140 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
972.5498972.5114393893970(R) (S)
974.5172974.4795393793860(K) (L)
1018.58221018.55332891991(K) (A)
1023.54531023.5111332092160(K) (E)
1028.60181028.5740272412490(R) (A)
1043.56691043.5485181241330(K) (L)
1089.58651089.55403051600(R) (L)
1119.55951119.5897-271451550(K) (R)
1182.64911182.6119321571660(R) (G)
1274.65731274.601744pyroGlu 4724820(R)QNGDDPLLTYR(F)
1320.63011320.6409-8.24284391(R) (V)
1320.63011320.6582-211Met-ox 1912011(R)VDAENRLQTMK(E)
1377.68321377.6334365165270(K) (T)
1475.78661475.7681131Met-ox 3673781(K)LALDMEIHAYRK(L)
1566.78471566.7512216286440(R) (S)
1605.83821605.8124164404531(R) (L)
1629.82681629.80841129411(R) (L)
1699.96341699.9707-4.32973111(R) (Q)
1752.88701752.8629142812960(R) (I)
1909.95121909.9217151Met-ox 3523660(R)MQQQLDEYQELLDIK(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 34% (232/664AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 12/96 matches (12%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
909.4435909.4543-12AcetN 181(-)SRQFSSR(S)
1033.49981033.5166-164844920(R) (M)
1179.63451179.6010283773860(K) (H)
1277.70931277.7105-0.984734830(K) (T)
1302.70091302.7018-0.663934031(R) (V)
1302.70091302.7157-113443550(R) (A)
1383.70891383.6909131861970(K) (V)
1475.78661475.7494252122230(K) (T)
1475.78661475.78580.502002110(R) (W)
1657.81071657.79341013290(R) (R)
1687.99701687.97071666821(R) (G)
1994.01491993.9772192242390(R) (R)
2150.12862150.0783232242401(R) (R)
2383.98732383.9524155195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (179/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 17/96 matches (17%).
Acc. #: Species: MOUSE Name: Lamins C and C2
Index: MW: 65447 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
972.5498972.5114393893970(R) (S)
974.5172974.4795393793860(K) (L)
1018.58221018.55332891991(K) (A)
1023.54531023.5111332092160(K) (E)
1028.60181028.5740272412490(R) (A)
1043.56691043.5485181241330(K) (L)
1089.58651089.55403051600(R) (L)
1119.55951119.5897-271451550(K) (R)
1182.64911182.6119321571660(R) (G)
1320.63011320.6409-8.24284391(R) (V)
1475.78661475.7681131Met-ox 3673781(K)LALDMEIHAYRK(L)
1605.83821605.8124164404531(R) (L)
1629.82681629.80841129411(R) (L)
1699.96341699.9707-4.32973111(R) (Q)
1707.79331707.8050-6.94214351(K) (T)
1752.88701752.8629142812960(R) (I)
1909.95121909.9217151Met-ox 3523660(R)MQQQLDEYQELLDIK(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (188/574AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 9/96 matches (9%).
Acc. #: Species: HUMAN Name: Bifunctional purine biosynthesis protein PURH [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR transformylase); IMP cyclohydrolase (Inosinicase) (IMP synthetase) (ATIC)]
Index: MW: 64466 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1008.53181008.5056264614670(K) (H)
1163.65651163.6424122943040(K) (A)
1323.67671323.7135-28971070(R) (T)
1464.77531464.7334295535680(R) (V)
1623.82001623.7688321Met-ox 49630(R)DVSELTGFPEMLGGR(V)
1715.89141715.8645165305440(K) (D)
2034.94962034.9197151771930(K) (K)
2150.12862150.1643-175695870(K) (L)
2553.37642553.3776-0.481081320(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (136/591AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 11/96 matches (11%).
Acc. #: Species: HUMAN Name: Splicing factor 3B subunit 2 (Spliceosome associated protein 145) (SAP 145) (SF3b150) (Pre-mRNA splicing factor SF3b 145 kDa subunit)
Index: MW: 97657 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
909.4435909.43529.21Met-ox 5215271(K)EEQKTMK(S)
1028.60181028.5740271611690(R) (Q)
1214.62911214.62037.21120(-) (L)
1232.63051232.6315-0.886166260(R) (I)
1235.63791235.5980323163271(R) (E)
1307.71651307.7298-106056150(K) (Y)
1323.67671323.7247-361Met-ox 6056150(K)VPPPWLIAMQR(Y)
1629.82681629.8892-384935060(R) (R)
1752.88701752.8961-5.25415541(K) (W)
1940.96281940.8936362792970(R) (K)
1994.01491994.0671-2613301(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (127/872AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 9/96 matches (9%).
Acc. #: Species: HUMAN Name: Adseverin (Scinderin)
Index: MW: 80509 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
900.5498900.50964562680(R) (E)
1028.60181028.5853164634720(R) (V)
1182.64911182.6887-334784870(K) (D)
1277.70931277.7469-293593691(K) (Q)
1493.79031493.7276421501620(R) (G)
1837.99211837.97728.11191370(K) (R)
1870.96431870.9915-153253410(K) (Q)
1994.01491994.0783-321191381(K) (L)
2097.03792096.9889235986150(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (106/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 10/96 matches (10%).
Acc. #: Species: HUMAN Name: Dystrobrevin alpha (Dystrobrevin-alpha)
Index: MW: 83935 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1028.60181028.5740274854920(R) (L)
1235.63791235.6384-0.433914010(K) (L)
1298.69781298.7181-164834921(K) (L)
1493.79031493.7236454955070(R) (A)
1608.89291608.8379342993131(K) (E)
1691.84031691.8023225265391(K) (R)
1707.79331707.7972-2.31Met-ox 5265391(K)DELEQRMSALQESR(R)
1921.91251921.9217-4.8pyroGlu 1Met-ox 7177311(R)QLENELQMEEYLKQK(L)
2081.05152081.0191161771950(K) (S)
2199.01762198.9689226346530(K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (117/743AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 9/96 matches (9%).
Acc. #: Species: MOUSE Name: Glutamate receptor, ionotropic kainate 1 precursor (Glutamate receptor 5) (GluR-5) (GluR5)
Index: MW: 95203 Da pI: 8.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1033.49981033.4665326876950(R) (K)
1298.69781298.63814684940(R) (R)
1475.78661475.7203454204321(R) (T)
1502.84411502.8583-9.45105230(R) (E)
1510.78911510.8157-184704820(K) (L)
1752.88701752.8089451Met-ox 6997121(K)ISTYEKMWAFMSSR(Q)
1838.94701838.93964.07868010(K) (N)
1851.97171851.9228263874021(K) (I)
1940.96281941.0407-404334480(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (122/836AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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