MS-Fit Search Results

Parameters

Database searched: SwissProt.10.07.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D186
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 114561
Molecular weight search (1000 - 100000 Da) selects 108217 entries.
Full pI range: 114561 entries.
Species search ( HUMAN MOUSE ) selects 14035 entries.
Combined molecular weight, pI and species searches select 12424 entries.
Pre searches select 12424 entries.

Data Set 1 Results

MS-Fit search selects 1139 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/89(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	3.348e+010	22 (24)	38.0	24.7	3.48	29.2	60631/6.3	MOUSE	T-COMPLEX PROTEIN 1, GAMMA SUBUNIT (TCP-1-GAMMA) (CCT-GAMMA) (MATRICIN)
2	1.983e+010	22 (24)	38.0	24.7	4.37	30.2	60403/6.1	HUMAN	T-complex protein 1, gamma subunit (TCP-1-gamma) (CCT-gamma)
3	1.183e+005	15 (16)	24.0	16.9	-8.50	45.7	74210/6.5	MOUSE	Lamin A
4	4.733e+004	7 (7)	33.0	7.9	-9.32	53.1	22614/7.5	HUMAN	ARF-related protein (ARP)
5	2.217e+004	14 (15)	24.0	15.7	-6.04	43.1	65447/6.4	MOUSE	Lamins C and C2

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110870	.	.	.	x	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	x	x	x	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 22/89 matches (24%).
Acc. #: Species: MOUSE Name: T-COMPLEX PROTEIN 1, GAMMA SUBUNIT (TCP-1-GAMMA) (CCT-GAMMA) (MATRICIN)
Index: MW: 60631 Da pI: 6.3

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
974.4961	974.5159	-20		382	389	0	(K) (N)
1120.5855	1120.6115	-23		69	78	0	(R) (S)
1166.6909	1166.6897	0.98		439	449	0	(R) (T)
1185.7198	1185.7207	-0.73		508	518	0	(K) (I)
1242.6826	1242.6806	1.6		139	149	1	(K) (E)
1248.6323	1248.5829	40		390	399	0	(R) (N)
1248.6323	1248.6072	20		250	260	0	(K) (E)
1254.6798	1254.7098	-24		492	502	0	(K) (L)
1264.5925	1264.5778	12	1Met-ox	390	399	0	(R)NLQDAMQVCR(N)
1337.6590	1337.6312	21		428	438	0	(K) (A)
1347.7124	1347.7055	5.2		450	461	0	(R) (L)
1353.6333	1353.6261	5.3	1Met-ox	428	438	0	(K)AMTGVEQWPYR(A)
1403.7157	1403.7105	3.7		295	306	0	(K) (A)
1416.7030	1416.7222	-14		340	353	0	(R) (K)
1419.7022	1419.7055	-2.3	1Met-ox	295	306	0	(K)GISDLAQHYLMR(A)
1442.7631	1442.7677	-3.2		204	216	0	(K) (G)
1561.8079	1561.8048	2.0		168	181	0	(R) (T)
1568.7765	1568.7531	15	1Met-ox	426	438	1	(K)SKAMTGVEQWPYR(A)
1798.9793	1798.9737	3.1		201	216	1	(R) (G)
1962.9365	1962.9159	11		355	370	1	(K) (A)
2163.0734	2163.0612	5.7	2Met-ox	49	68	0	(K)MLLDPMGGIVMTNDGNAILR(E)
2179.0874	2179.0561	14	3Met-ox	49	68	0	(K)MLLDPMGGIVMTNDGNAILR(E)
2496.3505	2496.3310	7.8		331	353	1	(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 38% (209/545AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 22/89 matches (24%).
Acc. #: Species: HUMAN Name: T-complex protein 1, gamma subunit (TCP-1-gamma) (CCT-gamma)
Index: MW: 60403 Da pI: 6.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
974.4961 974.5159 -20 381 388 0 (K) (N)
1120.5855 1120.6115 -23 68 77 0 (R) (S)
1125.5247 1125.4999 22 1Met-ox 181 189 0 (K)MVQFEENGR(K)
1166.6909 1166.6897 0.98 438 448 0 (R) (T)
1185.7198 1185.7207 -0.73 507 517 0 (K) (I)
1248.6323 1248.5829 40 389 398 0 (R) (N)
1248.6323 1248.6072 20 249 259 0 (K) (E)
1254.6798 1254.7098 -24 491 501 0 (K) (L)
1264.5925 1264.5778 12 1Met-ox 389 398 0 (R)NLQDAMQVCR(N)
1337.6590 1337.6312 21 427 437 0 (K) (A)
1347.7124 1347.7055 5.2 449 460 0 (R) (L)
1353.6333 1353.6261 5.3 1Met-ox 427 437 0 (K)AMTGVEQWPYR(A)
1403.7157 1403.7105 3.7 294 305 0 (K) (A)
1416.7030 1416.7222 -14 339 352 0 (R) (K)
1419.7022 1419.7055 -2.3 1Met-ox 294 305 0 (K)GISDLAQHYLMR(A)
1442.7631 1442.7677 -3.2 203 215 0 (K) (G)
1561.8079 1561.8048 2.0 167 180 0 (R) (M)
1568.7765 1568.7531 15 1Met-ox 425 437 1 (K)SKAMTGVEQWPYR(A)
1798.9793 1798.9737 3.1 200 215 1 (R) (G)
1962.9365 1962.9159 11 354 369 1 (K) (A)
2163.0734 2163.0612 5.7 2Met-ox 48 67 0 (K)MLLDPMGGIVMTNDGNAILR(E)
2179.0874 2179.0561 14 3Met-ox 48 67 0 (K)MLLDPMGGIVMTNDGNAILR(E)
2496.3505 2496.3310 7.8 330 352 1 (R) (K)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
974.4961	974.5159	-20		381	388	0	(K) (N)
1120.5855	1120.6115	-23		68	77	0	(R) (S)
1125.5247	1125.4999	22	1Met-ox	181	189	0	(K)MVQFEENGR(K)
1166.6909	1166.6897	0.98		438	448	0	(R) (T)
1185.7198	1185.7207	-0.73		507	517	0	(K) (I)
1248.6323	1248.5829	40		389	398	0	(R) (N)
1248.6323	1248.6072	20		249	259	0	(K) (E)
1254.6798	1254.7098	-24		491	501	0	(K) (L)
1264.5925	1264.5778	12	1Met-ox	389	398	0	(R)NLQDAMQVCR(N)
1337.6590	1337.6312	21		427	437	0	(K) (A)
1347.7124	1347.7055	5.2		449	460	0	(R) (L)
1353.6333	1353.6261	5.3	1Met-ox	427	437	0	(K)AMTGVEQWPYR(A)
1403.7157	1403.7105	3.7		294	305	0	(K) (A)
1416.7030	1416.7222	-14		339	352	0	(R) (K)
1419.7022	1419.7055	-2.3	1Met-ox	294	305	0	(K)GISDLAQHYLMR(A)
1442.7631	1442.7677	-3.2		203	215	0	(K) (G)
1561.8079	1561.8048	2.0		167	180	0	(R) (M)
1568.7765	1568.7531	15	1Met-ox	425	437	1	(K)SKAMTGVEQWPYR(A)
1798.9793	1798.9737	3.1		200	215	1	(R) (G)
1962.9365	1962.9159	11		354	369	1	(K) (A)
2163.0734	2163.0612	5.7	2Met-ox	48	67	0	(K)MLLDPMGGIVMTNDGNAILR(E)
2179.0874	2179.0561	14	3Met-ox	48	67	0	(K)MLLDPMGGIVMTNDGNAILR(E)
2496.3505	2496.3310	7.8		330	352	1	(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 38% (207/544AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 15/89 matches (16%).
Acc. #: Species: MOUSE Name: Lamin A
Index: MW: 74210 Da pI: 6.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
974.4961 974.4795 17 379 386 0 (K) (L)
1023.5066 1023.5111 -4.4 209 216 0 (K) (E)
1028.5473 1028.5740 -26 241 249 0 (R) (A)
1171.5764 1171.5894 -11 pyroGlu 182 190 1 (K)QLQDEMLRR(V)
1188.6550 1188.6159 33 182 190 1 (K) (V)
1320.5987 1320.6409 -32 428 439 1 (R) (V)
1347.7124 1347.6731 29 1Met-ox 367 377 0 (K)LALDMEIHAYR(K)
1475.7195 1475.7681 -33 1Met-ox 367 378 1 (K)LALDMEIHAYRK(L)
1605.7742 1605.8124 -24 440 453 1 (R) (L)
1629.7997 1629.8084 -5.3 29 41 1 (R) (L)
1699.9370 1699.9707 -20 297 311 1 (R) (Q)
1707.8069 1707.8050 1.1 421 435 1 (K) (T)
1752.8781 1752.8629 8.7 281 296 0 (R) (I)
1909.8869 1909.9217 -18 1Met-ox 352 366 0 (R)MQQQLDEYQELLDIK(L)
2193.9756 2194.0700 -43 600 625 0 (K) (S)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
974.4961	974.4795	17		379	386	0	(K) (L)
1023.5066	1023.5111	-4.4		209	216	0	(K) (E)
1028.5473	1028.5740	-26		241	249	0	(R) (A)
1171.5764	1171.5894	-11	pyroGlu	182	190	1	(K)QLQDEMLRR(V)
1188.6550	1188.6159	33		182	190	1	(K) (V)
1320.5987	1320.6409	-32		428	439	1	(R) (V)
1347.7124	1347.6731	29	1Met-ox	367	377	0	(K)LALDMEIHAYR(K)
1475.7195	1475.7681	-33	1Met-ox	367	378	1	(K)LALDMEIHAYRK(L)
1605.7742	1605.8124	-24		440	453	1	(R) (L)
1629.7997	1629.8084	-5.3		29	41	1	(R) (L)
1699.9370	1699.9707	-20		297	311	1	(R) (Q)
1707.8069	1707.8050	1.1		421	435	1	(K) (T)
1752.8781	1752.8629	8.7		281	296	0	(R) (I)
1909.8869	1909.9217	-18	1Met-ox	352	366	0	(R)MQQQLDEYQELLDIK(L)
2193.9756	2194.0700	-43		600	625	0	(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (164/665AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/89 matches (7%).
Acc. #: Species: HUMAN Name: ARF-related protein (ARP)
Index: MW: 22614 Da pI: 7.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1030.4531 1030.4515 1.5 149 157 0 (K) (I)
1188.6550 1188.6339 18 1 10 0 (-) (Y)
1403.7157 1403.7806 -46 186 196 1 (K) (Q)
1707.8069 1707.8627 -33 16 30 0 (K) (T)
1812.9224 1812.9246 -1.2 AcetN 1Met-ox 1 15 1 (-)YTLLSGLYKYMFQK(D)
2194.9878 2195.0483 -28 71 88 0 (R) (Y)
2211.0946 2211.0432 23 1Met-ox 71 88 0 (R)LMFWDLGGQEELQSLWDK(Y)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1030.4531	1030.4515	1.5		149	157	0	(K) (I)
1188.6550	1188.6339	18		1	10	0	(-) (Y)
1403.7157	1403.7806	-46		186	196	1	(K) (Q)
1707.8069	1707.8627	-33		16	30	0	(K) (T)
1812.9224	1812.9246	-1.2	AcetN 1Met-ox	1	15	1	(-)YTLLSGLYKYMFQK(D)
2194.9878	2195.0483	-28		71	88	0	(R) (Y)
2211.0946	2211.0432	23	1Met-ox	71	88	0	(R)LMFWDLGGQEELQSLWDK(Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 33% (68/201AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 14/89 matches (15%).
Acc. #: Species: MOUSE Name: Lamins C and C2
Index: MW: 65447 Da pI: 6.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
974.4961 974.4795 17 379 386 0 (K) (L)
1023.5066 1023.5111 -4.4 209 216 0 (K) (E)
1028.5473 1028.5740 -26 241 249 0 (R) (A)
1171.5764 1171.5894 -11 pyroGlu 182 190 1 (K)QLQDEMLRR(V)
1188.6550 1188.6159 33 182 190 1 (K) (V)
1320.5987 1320.6409 -32 428 439 1 (R) (V)
1347.7124 1347.6731 29 1Met-ox 367 377 0 (K)LALDMEIHAYR(K)
1475.7195 1475.7681 -33 1Met-ox 367 378 1 (K)LALDMEIHAYRK(L)
1605.7742 1605.8124 -24 440 453 1 (R) (L)
1629.7997 1629.8084 -5.3 29 41 1 (R) (L)
1699.9370 1699.9707 -20 297 311 1 (R) (Q)
1707.8069 1707.8050 1.1 421 435 1 (K) (T)
1752.8781 1752.8629 8.7 281 296 0 (R) (I)
1909.8869 1909.9217 -18 1Met-ox 352 366 0 (R)MQQQLDEYQELLDIK(L)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
974.4961	974.4795	17		379	386	0	(K) (L)
1023.5066	1023.5111	-4.4		209	216	0	(K) (E)
1028.5473	1028.5740	-26		241	249	0	(R) (A)
1171.5764	1171.5894	-11	pyroGlu	182	190	1	(K)QLQDEMLRR(V)
1188.6550	1188.6159	33		182	190	1	(K) (V)
1320.5987	1320.6409	-32		428	439	1	(R) (V)
1347.7124	1347.6731	29	1Met-ox	367	377	0	(K)LALDMEIHAYR(K)
1475.7195	1475.7681	-33	1Met-ox	367	378	1	(K)LALDMEIHAYRK(L)
1605.7742	1605.8124	-24		440	453	1	(R) (L)
1629.7997	1629.8084	-5.3		29	41	1	(R) (L)
1699.9370	1699.9707	-20		297	311	1	(R) (Q)
1707.8069	1707.8050	1.1		421	435	1	(K) (T)
1752.8781	1752.8629	8.7		281	296	0	(R) (I)
1909.8869	1909.9217	-18	1Met-ox	352	366	0	(R)MQQQLDEYQELLDIK(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (138/574AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):