MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): F02B
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( MAMMALS ) selects 20817 entries.
Combined molecular weight, pI and species searches select 18748 entries.
Pre searches select 18748 entries.

Data Set 1 Results

MS-Fit search selects 430 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/39(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.490e+01224 (61)41.061.5-1.7040.3 69294/5.8BOVINSerum albumin precursor (Allergen Bos d 6)
22.180e+00612 (30)22.030.86.8860.8 69189/5.8SHEEPSerum albumin precursor
336626 (15)10.015.44.19124 77359/8.3CAPHILactotransferrin precursor (Lactoferrin)
413847 (17)12.017.914.2121 84401/6.1BOVINIntegrin beta-2 precursor (Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 beta-subunit) (CD18) (Complement receptor C3 beta-subunit)
510519 (23)13.023.1-42.198.1 96950/8.2MOUSEDNA TOPOISOMERASE III BETA-1
79329......x.xx.xxxxxxxxx.x.x..xxxx.x.xxx.xx
30161............x.xxxxx...x..x...x.x.x....x
37219..................x.x....x..x....xx....
87412.........x..x....x.......x....x.....x.x
53438.....x..x....x...x...x.....x.....xx...x


Detailed Results

1. 24/39 matches (61%).
Acc. #: Species: BOVIN Name: Serum albumin precursor (Allergen Bos d 6)
Index: MW: 69294 Da pI: 5.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
927.4732927.4940-221611670(K) (R)
1048.40621048.4886-791231300(R) (D)
1065.42481065.4311-5.9pyroGlu 4134200(K)QNCDQFEK(L)
1163.60801163.6312-2066750(K) (T)
1166.54861166.5298164995070(K) (R)
1249.61931249.6217-1.935441(R) (G)
1283.71231283.71120.833613710(R) (L)
1305.71611305.7167-0.424024120(K) (Q)
1415.68261415.6881-3.81Met-ox 5695800(K)TVMENFVAFVDK(C)
1433.71501433.70993.6891000(K) (V)
1439.80891439.8123-2.43603711(R) (L)
1479.80191479.79604.04214330(K) (Y)
1530.65021530.64563.03753860(K) (D)
1567.75361567.74336.63473590(K) (R)
1639.94351639.93833.24374511(R) (S)
1670.76671670.75974.2pyroGlu 1181301(K)QEPERNECFLSHK(D)
1687.75091687.7862-211181301(K) (D)
1754.84871754.84571.71Met-ox 4694820(R)MPCTEDYLSLILNR(L)
1791.76471791.7100312672800(K) (A)
1888.94451888.92749.01691830(R) (Y)
1894.95011894.93736.85085230(R) (A)
1921.93291921.9370-2.15295440(K) (Q)
2034.01132033.9854131391551(K) (K)
2045.06002045.0285151681831(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 41% (250/607AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/39 matches (30%).
Acc. #: Species: SHEEP Name: Serum albumin precursor
Index: MW: 69189 Da pI: 5.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1166.54861166.5298164995070(K) (R)
1283.71231283.71120.833613710(R) (L)
1305.71611305.6183753003090(K) (S)
1305.71611305.7167-0.424024120(K) (K)
1415.68261415.6881-3.81Met-ox 5695800(K)TVMENFVAFVDK(C)
1433.71501433.70993.6891000(K) (V)
1433.71501433.8116-674024131(K) (N)
1439.80891439.8123-2.43603711(R) (L)
1566.73541566.6899293873990(K) (L)
1595.75721595.7746-113473590(K) (R)
1754.84871754.84571.71Met-ox 4694820(R)MPCTEDYLSLILNR(L)
1791.76471791.7100312672800(K) (A)
1888.94451888.92749.01691830(R) (Y)
2045.06002045.0285151681831(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (137/607AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/39 matches (15%).
Acc. #: Species: CAPHI Name: Lactotransferrin precursor (Lactoferrin)
Index: MW: 77359 Da pI: 8.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1439.80891439.6919813023140(K) (R)
1501.78001501.6819651711830(R) (A)
1595.75721595.7930-223023151(K) (D)
1687.75091687.8702-712632770(K) (S)
1888.94451889.0397-506236390(R) (N)
1894.95011894.9081222412551(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (74/708AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/39 matches (17%).
Acc. #: Species: BOVIN Name: Integrin beta-2 precursor (Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 beta-subunit) (CD18) (Complement receptor C3 beta-subunit)
Index: MW: 84401 Da pI: 6.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1065.42481065.5250-941Met-ox 7567650(K)SATTTVMNPK(F)
1166.54861166.5112324894980(R) (K)
1433.71501433.70258.749610(K) (C)
1595.75721595.7917-222102231(K) (Q)
1789.87921789.7195895645770(R) (S)
1993.95391993.9184185785951(K) (C)
2045.06002044.9221676646791(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (95/769AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 9/39 matches (23%).
Acc. #: Species: MOUSE Name: DNA TOPOISOMERASE III BETA-1
Index: MW: 96950 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
845.3174845.3874-836246291(R) (F)
1048.40621048.5063-952822900(K) (K)
1249.61931249.7017-66pyroGlu 3693790(K)QLLAEGINRPR(K)
1433.71501433.7939-55961071(K) (G)
1530.65021530.7916-922522631(K) (V)
1670.76671670.75586.51081200(R) (E)
1888.94451888.8978251Met-ox 2642780(R)VFDWEIAQMFLNMTK(L)
1894.95011894.9009266666810(R) (G)
2045.06002045.1503-441191(-) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (113/862AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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