MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): F03B
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( MAMMALS ) selects 20817 entries.
Combined molecular weight, pI and species searches select 18748 entries.
Pre searches select 18748 entries.

Data Set 1 Results

MS-Fit search selects 145 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/32(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	6.383e+008	16 (50)	29.0	50.0	-59.7	37.8		69294/5.8		BOVIN	Serum albumin precursor (Allergen Bos d 6)
2	1.633e+004	6 (18)	15.0	18.8	-63.9	36.7		66018/8.2		HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
3	1.602e+004	9 (28)	16.0	28.1	-49.4	46.2		69189/5.8		SHEEP	Serum albumin precursor
4	880	7 (21)	11.0	21.9	-56.2	75.1		97287/6.7		MOUSE	Glycogen phosphorylase, muscle form (Myophosphorylase)
5	664	5 (15)	9.0	15.6	-17.7	115		84401/6.1		BOVIN	Integrin beta-2 precursor (Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 beta-subunit) (CD18) (Complement receptor C3 beta-subunit)

79329	.	.	.	x	.	.	.	x	.	x	x	.	x	x	x	.	x	.	x	.	x	x	x	.	.	x	x	x	.	.	x	.
45313	.	.	.	.	x	.	.	.	x	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x
30161	.	.	.	.	.	.	.	.	.	x	x	.	x	x	x	.	.	x	.	.	.	.	x	.	.	x	.	.	.	.	x	.
18650	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	x	.	x	.	.	.	.	.	.	.	x	.	x	.	x	.	.
87412	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	x	.

Detailed Results

1. 16/32 matches (50%).
Acc. #: Species: BOVIN Name: Serum albumin precursor (Allergen Bos d 6)
Index: MW: 69294 Da pI: 5.8

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
927.4030	927.4940	-98		161	167	0	(K) (R)
1163.5379	1163.6312	-80		66	75	0	(K) (T)
1283.6331	1283.7112	-61		361	371	0	(R) (L)
1305.6167	1305.7167	-77		402	412	0	(K) (Q)
1415.5846	1415.6881	-73	1Met-ox	569	580	0	(K)TVMENFVAFVDK(C)
1433.6176	1433.7099	-64		89	100	0	(K) (V)
1439.7274	1439.8123	-59		360	371	1	(R) (L)
1479.7023	1479.7960	-63		421	433	0	(K) (Y)
1567.6581	1567.7433	-54		347	359	0	(K) (R)
1590.6627	1590.7838	-76		139	151	0	(K) (A)
1639.8475	1639.9383	-55		437	451	1	(R) (S)
1754.7494	1754.8457	-55	1Met-ox	469	482	0	(R)MPCTEDYLSLILNR(L)
1888.8529	1888.9274	-39		169	183	0	(R) (Y)
1894.8598	1894.9373	-41		508	523	0	(R) (A)
1921.8838	1921.9370	-28		529	544	0	(K) (Q)
2044.9659	2045.0285	-31		168	183	1	(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 29% (180/607AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/32 matches (18%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1033.4231 1033.5166 -90 484 492 0 (R) (M)
1179.5444 1179.6010 -48 377 386 0 (K) (H)
1383.6057 1383.6909 -62 186 197 0 (K) (V)
1475.6600 1475.7494 -61 212 223 0 (K) (T)
1475.6600 1475.7858 -85 200 211 0 (R) (W)
1993.8533 1993.9772 -62 224 239 0 (R) (R)
2383.8578 2383.9524 -40 519 549 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (102/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/32 matches (28%).
Acc. #: Species: SHEEP Name: Serum albumin precursor
Index: MW: 69189 Da pI: 5.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1283.6331 1283.7112 -61 361 371 0 (R) (L)
1305.6167 1305.6183 -1.2 300 309 0 (K) (S)
1305.6167 1305.7167 -77 402 412 0 (K) (K)
1415.5846 1415.6881 -73 1Met-ox 569 580 0 (K)TVMENFVAFVDK(C)
1433.6176 1433.7099 -64 89 100 0 (K) (V)
1439.7274 1439.8123 -59 360 371 1 (R) (L)
1566.6370 1566.6899 -34 387 399 0 (K) (L)
1754.7494 1754.8457 -55 1Met-ox 469 482 0 (R)MPCTEDYLSLILNR(L)
1888.8529 1888.9274 -39 169 183 0 (R) (Y)
2044.9659 2045.0285 -31 168 183 1 (R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (100/607AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/32 matches (21%).
Acc. #: Species: MOUSE Name: Glycogen phosphorylase, muscle form (Myophosphorylase)
Index: MW: 97287 Da pI: 6.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1283.6331 1283.6306 2.0 784 793 1 (K) (N)
1305.6167 1305.6479 -24 546 555 1 (K) (V)
1475.6600 1475.7535 -63 360 371 1 (R) (T)
1566.6370 1566.7916 -99 257 270 0 (K) (N)
1888.8529 1888.9227 -37 372 387 0 (K) (W)
1921.8838 1922.0500 -86 296 311 1 (K) (F)
1993.8533 1994.0248 -86 45 61 1 (R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (95/842AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/32 matches (15%).
Acc. #: Species: BOVIN Name: Integrin beta-2 precursor (Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 beta-subunit) (CD18) (Complement receptor C3 beta-subunit)
Index: MW: 84401 Da pI: 6.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1179.5444 1179.6374 -79 430 440 0 (R) (V)
1433.6176 1433.7025 -59 49 61 0 (K) (C)
1789.8278 1789.7195 61 564 577 0 (R) (S)
1993.8533 1993.9184 -33 578 595 1 (K) (C)
2044.9659 2044.9221 21 664 679 1 (K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (72/769AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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