MS-Fit Search Results

Press stop on your browser if you wish to abort this MS-Fit search prematurely.
Sample ID (comment): F004
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 674 entries (results displayed for top 5 matches).

Parameters Used in Search

4	75.000 ppm	monoisotopic	Trypsin	4	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	3.6e+003	9/39 (23%)	57651.7 / 9.61	HUMAN	ZINC FINGER PROTEIN 169
2	518	9/39 (23%)	83295.0 / 4.97	HUMAN	HEAT SHOCK PROTEIN HSP 90-BETA (HSP 84) (HSP 90)
3	414	10/39 (25%)	89614.9 / 8.61	HUMAN	TUMOR NECROSIS FACTOR, ALPHA-INDUCED PROTEIN 3 (PUTATIVE DNA BINDING PROTEIN A20) (ZINC FINGER PROTEIN A20)
4	318	8/39 (20%)	83326.0 / 4.97	MOUSE	HEAT SHOCK PROTEIN HSP 90-BETA (HSP 84) (TUMOR SPECIFIC TRANSPLANTATION 84 KD ANTIGEN) (TSTA)
5	248	4/39 (10%)	16359.9 / 8.16	HUMAN	PHOSPHOLIPASE A2 PRECURSOR (PHOSPHATIDYLCHOLINE 2-ACYLHYDROLASE) (GROUP IB PHOSPHOLIPASE A2)

Detailed Results

1. 9/39 matches (23%). 57651.7 Da, pI = 9.61. Acc. # Q14929. HUMAN. ZINC FINGER PROTEIN 169.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1165.4875	1165.4995	-10.2664	143	151	(K)HHVCPECGR(G)	1Cys-am
1179.5019	1179.5152	-11.2797	143	151	(K)HHVCPECGR(G)	2Cys-am
1263.5805	1263.6486	-53.8637	502	512	(K)(-)
1790.7854	1790.7954	-5.5801	360	374	(R)(G)
1833.7166	1833.7682	-28.1479	444	458	(R)THSGEKPCICDECGR(G)	2Cys-am
1847.7091	1847.7839	-40.4370	444	458	(R)THSGEKPCICDECGR(G)	3Cys-am
1890.8725	1890.9067	-18.1069	193	207	(R)KHSGEKPYVCRECGR(H)	2Cys-am
2151.0272	2151.1158	-41.2190	113	133	(K)(K)
2383.9627	2384.1174	-64.8820	143	161	(K)HHVCPECGRGFCQRSDLIK(H)	2Cys-am
2383.9627	2384.0586	-40.1979	444	463	(R)THSGEKPCICDECGRGFGFK(S)	3Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 19% (101/512AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 23.1 Mean Error = -31.3981 ppm Data Tolerance = 39.9252 ppm Mean Missed Cleavages = 0.7

2. 9/39 matches (23%). 83295.0 Da, pI = 4.97. Acc. # P08238. HUMAN. HEAT SHOCK PROTEIN HSP 90-BETA (HSP 84) (HSP 90).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1264.5770 1264.5012 59.9275 613 623 (R)DNSTMGYMMAK(K) 1Met-ox
1348.5784 1348.6650 -64.2140 320 330 (K)(A)
1408.5942 1408.6960 -72.2353 274 284 (K)(T)
1408.5942 1408.5911 2.2432 613 624 (R)DNSTMGYMMAKK(H) 2Met-ox
1847.7091 1847.7976 -47.8521 292 306 (R)(S)
1993.9023 1993.9202 -8.9950 250 267 (K)(K)
2065.0912 2065.1293 -18.4636 65 82 (K)(T)
2176.9333 2176.9457 -5.7129 457 475 (R)(M)
2255.9190 2255.9594 -17.9005 149 168 (K)(A)
2383.9627 2384.0543 -38.4198 148 168 (R)(A)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1264.5770	1264.5012	59.9275	613	623	(R)DNSTMGYMMAK(K)	1Met-ox
1348.5784	1348.6650	-64.2140	320	330	(K)(A)
1408.5942	1408.6960	-72.2353	274	284	(K)(T)
1408.5942	1408.5911	2.2432	613	624	(R)DNSTMGYMMAKK(H)	2Met-ox
1847.7091	1847.7976	-47.8521	292	306	(R)(S)
1993.9023	1993.9202	-8.9950	250	267	(K)(K)
2065.0912	2065.1293	-18.4636	65	82	(K)(T)
2176.9333	2176.9457	-5.7129	457	475	(R)(M)
2255.9190	2255.9594	-17.9005	149	168	(K)(A)
2383.9627	2384.0543	-38.4198	148	168	(R)(A)

The matched peptides cover 17% (125/724AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 23.1 Mean Error = -21.1622 ppm Data Tolerance = 76.0120 ppm Mean Missed Cleavages = 0.8

3. 10/39 matches (25%). 89614.9 Da, pI = 8.61. Acc. # P21580. HUMAN. TUMOR NECROSIS FACTOR, ALPHA-INDUCED PROTEIN 3 (PUTATIVE DNA BINDING PROTEIN A20) (ZINC FINGER PROTEIN A20).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1036.4268 1036.4951 -65.8941 750 759 (R)(Q)
1165.4875 1165.5424 -47.0821 677 685 (K)(F)
1179.5019 1179.5581 -47.6579 677 685 (K)CFIEAQNQR(F) 1Cys-am
1264.5770 1264.5778 -0.6583 521 530 (K)CQACLQDVTR(T) 1Cys-am
1348.5784 1348.6030 -18.2385 531 541 (R)TFNGICSTCFK(R) 1Cys-am
1475.6427 1475.6841 -28.0505 288 299 (K)VHFLTDPENEMK(E) 1Met-ox
1790.7854 1790.9111 -70.1735 1 16 (-)AEQVLPQALYLSNMR(K) 1Met-ox Acet N
1833.7166 1833.7318 -8.3061 729 742 (R)SEELCMECQHPNQR(M) 1Met-ox
1847.7091 1847.7475 -20.7460 729 742 (R)SEELCMECQHPNQR(M) 1Met-ox 1Cys-am
1993.9023 1994.0500 -74.0745 25 41 (R)(T)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1036.4268	1036.4951	-65.8941	750	759	(R)(Q)
1165.4875	1165.5424	-47.0821	677	685	(K)(F)
1179.5019	1179.5581	-47.6579	677	685	(K)CFIEAQNQR(F)	1Cys-am
1264.5770	1264.5778	-0.6583	521	530	(K)CQACLQDVTR(T)	1Cys-am
1348.5784	1348.6030	-18.2385	531	541	(R)TFNGICSTCFK(R)	1Cys-am
1475.6427	1475.6841	-28.0505	288	299	(K)VHFLTDPENEMK(E)	1Met-ox
1790.7854	1790.9111	-70.1735	1	16	(-)AEQVLPQALYLSNMR(K)	1Met-ox Acet N
1833.7166	1833.7318	-8.3061	729	742	(R)SEELCMECQHPNQR(M)	1Met-ox
1847.7091	1847.7475	-20.7460	729	742	(R)SEELCMECQHPNQR(M)	1Met-ox 1Cys-am
1993.9023	1994.0500	-74.0745	25	41	(R)(T)

The matched peptides cover 12% (99/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.6 Mean Error = -38.0881 ppm Data Tolerance = 53.1397 ppm Mean Missed Cleavages = 0.0

4. 8/39 matches (20%). 83326.0 Da, pI = 4.97. Acc. # P11499. MOUSE. HEAT SHOCK PROTEIN HSP 90-BETA (HSP 84) (TUMOR SPECIFIC TRANSPLANTATION 84 KD ANTIGEN) (TSTA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1264.5770 1264.5012 59.9275 613 623 (R)DNSTMGYMMAK(K) 1Met-ox
1348.5784 1348.6650 -64.2140 320 330 (K)(A)
1408.5942 1408.6960 -72.2353 274 284 (K)(T)
1408.5942 1408.5911 2.2432 613 624 (R)DNSTMGYMMAKK(H) 2Met-ox
1847.7091 1847.7976 -47.8521 292 306 (R)(S)
1993.9023 1993.9202 -8.9950 250 267 (K)(K)
2065.0912 2065.1293 -18.4636 65 82 (K)(T)
2176.9333 2176.9457 -5.7129 457 475 (R)(M)
2255.9190 2255.9594 -17.9005 149 168 (K)(A)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1264.5770	1264.5012	59.9275	613	623	(R)DNSTMGYMMAK(K)	1Met-ox
1348.5784	1348.6650	-64.2140	320	330	(K)(A)
1408.5942	1408.6960	-72.2353	274	284	(K)(T)
1408.5942	1408.5911	2.2432	613	624	(R)DNSTMGYMMAKK(H)	2Met-ox
1847.7091	1847.7976	-47.8521	292	306	(R)(S)
1993.9023	1993.9202	-8.9950	250	267	(K)(K)
2065.0912	2065.1293	-18.4636	65	82	(K)(T)
2176.9333	2176.9457	-5.7129	457	475	(R)(M)
2255.9190	2255.9594	-17.9005	149	168	(K)(A)

The matched peptides cover 17% (124/724AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.5 Mean Error = -19.2447 ppm Data Tolerance = 79.5901 ppm Mean Missed Cleavages = 0.8

5. 4/39 matches (10%). 16359.9 Da, pI = 8.16. Acc. # P04054. HUMAN. PHOSPHOLIPASE A2 PRECURSOR (PHOSPHATIDYLCHOLINE 2-ACYLHYDROLASE) (GROUP IB PHOSPHOLIPASE A2).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1487.6526 1487.5717 54.3276 112 122 (K)ECEAFICNCDR(N) 1Cys-am
1515.6643 1515.6030 40.4031 112 122 (K)ECEAFICNCDR(N) 3Cys-am
1743.7366 1743.7253 6.4825 110 122 (K)NKECEAFICNCDR(N) 2Cys-am
2873.3354 2873.2762 20.5827 85 109 (K)FLLDNPYTHTYSYSCSGSAITCSSK(N) 1Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1487.6526	1487.5717	54.3276	112	122	(K)ECEAFICNCDR(N)	1Cys-am
1515.6643	1515.6030	40.4031	112	122	(K)ECEAFICNCDR(N)	3Cys-am
1743.7366	1743.7253	6.4825	110	122	(K)NKECEAFICNCDR(N)	2Cys-am
2873.3354	2873.2762	20.5827	85	109	(K)FLLDNPYTHTYSYSCSGSAITCSSK(N)	1Cys-am

The matched peptides cover 25% (38/148AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 10.3 Mean Error = 30.4490 ppm Data Tolerance = 42.2849 ppm Mean Missed Cleavages = 0.3