MS-Fit Search Results

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Sample ID (comment): T001
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 408 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	75.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.15e+007	15/28 (53%)	28804.2 / 6.68	HUMAN	PHOSPHOGLYCERATE MUTASE, BRAIN FORM (PGAM-B) (BPG-DEPENDENT PGAM)
2	215	6/28 (21%)	56888.9 / 8.53	HUMAN	HISTIDYL-TRNA SYNTHETASE HOMOLOG (HISTIDINE--TRNA LIGASE HOMOLOG) (HISRS)
3	194	5/28 (17%)	42111.6 / 5.55	MOUSE	MASPIN PRECURSOR (PROTEASE INHIBITOR 5)
4	132	5/28 (17%)	98978.0 / 5.65	MOUSE	INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN H3 PRECURSOR (ITI HEAVY CHAIN H3)
5	125	4/28 (14%)	40080.1 / 8.84	MOUSE	LIM/HOMEOBOX PROTEIN LHX6.1

Detailed Results

1. 15/28 matches (53%). 28804.2 Da, pI = 6.68. Acc. # P18669. HUMAN. PHOSPHOGLYCERATE MUTASE, BRAIN FORM (PGAM-B) (BPG-DEPENDENT PGAM).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
613.4266	613.4401	-22.0194	6	10	(K)(H)
1150.7044	1150.6697	30.1880	181	191	(R)(G)
1312.6427	1312.6034	29.9157	11	21	(R)(F)
1683.9652	1683.9110	32.1946	163	176	(R)(E)
1793.8476	1793.8056	23.4115	47	61	(R)DAGYEFDICFTSVQK(R)	1Cys-am
1949.9961	1949.9067	45.8318	47	62	(R)DAGYEFDICFTSVQKR(A)	1Cys-am
1979.9625	1979.8775	42.9008	22	39	(R)(R)
2115.2333	2115.1272	50.1260	223	240	(K)(K)
2131.2209	2131.1221	46.3348	223	240	(K)NLKPIKPMQFLGDEETVR(K)	1Met-ox
2136.0808	2135.9787	47.8288	22	40	(R)(G)
2172.2393	2172.1527	39.8689	66	83	(R)TLWTVLDAIDQMWLPVVR(T)	1Met-ox
2417.2497	2417.1124	56.8116	118	138	(R)(D)
2433.2275	2433.1073	49.4065	118	138	(R)SYDVPPPPMEPDHPFYSNISK(D)	1Met-ox
2439.3176	2439.1713	59.9599	142	162	(R)YADLTEDQLPSCESLKDTIAR(A)	1Cys-am
2589.3725	2589.2084	63.3882	117	138	(R)RSYDVPPPPMEPDHPFYSNISK(D)	1Met-ox

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The matched peptides cover 61% (155/254AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 53.6 Mean Error = 39.7432 ppm Data Tolerance = 41.2047 ppm Mean Missed Cleavages = 0.3

2. 6/28 matches (21%). 56888.9 Da, pI = 8.53. Acc. # P49590. HUMAN. HISTIDYL-TRNA SYNTHETASE HOMOLOG (HISTIDINE--TRNA LIGASE HOMOLOG) (HISRS).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1081.5555 1081.5682 -11.7970 130 138 (R)(Y)
1344.6871 1344.7599 -54.1749 481 492 (R)(E)
1949.9961 1950.0444 -24.7747 195 211 (K)(V)
1979.9625 1980.0550 -46.7146 195 211 (K)IMCEILSGLQLGDFLIK(V) 1Met-ox 1Cys-am
1979.9625 1980.0516 -45.0123 428 444 (K)(N)
2136.0808 2136.0946 -6.4308 234 251 (R)(H)
2433.2275 2433.2852 -23.7101 428 448 (K)(L)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1081.5555	1081.5682	-11.7970	130	138	(R)(Y)
1344.6871	1344.7599	-54.1749	481	492	(R)(E)
1949.9961	1950.0444	-24.7747	195	211	(K)(V)
1979.9625	1980.0550	-46.7146	195	211	(K)IMCEILSGLQLGDFLIK(V)	1Met-ox 1Cys-am
1979.9625	1980.0516	-45.0123	428	444	(K)(N)
2136.0808	2136.0946	-6.4308	234	251	(R)(H)
2433.2275	2433.2852	-23.7101	428	448	(K)(L)

The matched peptides cover 15% (77/506AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 21.4 Mean Error = -30.3735 ppm Data Tolerance = 36.8955 ppm Mean Missed Cleavages = 1.1

3. 5/28 matches (17%). 42111.6 Da, pI = 5.55. Acc. # P70124. MOUSE. MASPIN PRECURSOR (PROTEASE INHIBITOR 5).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1081.5555 1081.6118 -52.1031 312 321 (K)(V)
1150.7044 1150.7022 1.9038 225 234 (K)(D)
1979.9625 1980.0278 -32.9854 360 375 (R)HNKTRNIIFFGKFCSP(-) 1Cys-am
2439.3176 2439.2771 16.5897 110 129 (K)(G)
2589.3725 2589.3598 4.9086 23 47 (R)(G)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1081.5555	1081.6118	-52.1031	312	321	(K)(V)
1150.7044	1150.7022	1.9038	225	234	(K)(D)
1979.9625	1980.0278	-32.9854	360	375	(R)HNKTRNIIFFGKFCSP(-)	1Cys-am
2439.3176	2439.2771	16.5897	110	129	(K)(G)
2589.3725	2589.3598	4.9086	23	47	(R)(G)

The matched peptides cover 21% (81/375AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.9 Mean Error = -12.3373 ppm Data Tolerance = 57.8331 ppm Mean Missed Cleavages = 1.2

4. 5/28 matches (17%). 98978.0 Da, pI = 5.65. Acc. # Q61704. MOUSE. INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN H3 PRECURSOR (ITI HEAVY CHAIN H3).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1949.9961 1950.0296 -17.1919 104 120 (K)(T)
1979.9625 1980.1105 -74.7327 162 177 (K)(H)
2115.2333 2115.1272 50.1260 817 836 (K)(N)
2131.2209 2131.1221 46.3348 817 836 (K)VFGIRPGSDPTKPDATMVVK(N) 1Met-ox
2433.2275 2433.2463 -7.7286 1 21 (-)(S)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1949.9961	1950.0296	-17.1919	104	120	(K)(T)
1979.9625	1980.1105	-74.7327	162	177	(K)(H)
2115.2333	2115.1272	50.1260	817	836	(K)(N)
2131.2209	2131.1221	46.3348	817	836	(K)VFGIRPGSDPTKPDATMVVK(N)	1Met-ox
2433.2275	2433.2463	-7.7286	1	21	(-)(S)

The matched peptides cover 8% (74/886AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.9 Mean Error = -0.6385 ppm Data Tolerance = 102.9454 ppm Mean Missed Cleavages = 1.2

5. 4/28 matches (14%). 40080.1 Da, pI = 8.84. Acc. # Q9R1R0. MOUSE. LIM/HOMEOBOX PROTEIN LHX6.1.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1150.7044 1150.6485 48.5515 85 93 (K)(C)
1344.6871 1344.6759 8.2936 345 357 (K)(V)
2417.2497 2417.1329 48.3393 14 37 (R)(D)
2433.2275 2433.1278 40.9900 14 37 (R)CLEGTTPPAMAQSDAEALAGALDK(D) 1Met-ox
2433.2275 2433.2019 10.5316 102 120 (R)(M)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1150.7044	1150.6485	48.5515	85	93	(K)(C)
1344.6871	1344.6759	8.2936	345	357	(K)(V)
2417.2497	2417.1329	48.3393	14	37	(R)(D)
2433.2275	2433.1278	40.9900	14	37	(R)CLEGTTPPAMAQSDAEALAGALDK(D)	1Met-ox
2433.2275	2433.2019	10.5316	102	120	(R)(M)

The matched peptides cover 17% (65/363AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 14.3 Mean Error = 31.3412 ppm Data Tolerance = 40.5273 ppm Mean Missed Cleavages = 0.8