MS-Fit Search Results

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Sample ID (comment): T003
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 410 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	75.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	3.62e+004	10/24 (41%)	21892.1 / 5.66	HUMAN	PEROXIREDOXIN 2 (THIOREDOXIN PEROXIDASE 1) (THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE 1) (THIOL-SPECIFIC ANTIOXIDANT PROTEIN) (TSA) (PRP) (NATURAL KILLER CELL ENHANCING FACTOR B) (NKEF-B)
2	299	6/24 (25%)	21778.8 / 5.20	MOUSE	PEROXIREDOXIN 2 (THIOREDOXIN PEROXIDASE 1) (THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE 1) (THIOL-SPECIFIC ANTIOXIDANT PROTEIN) (TSA)
3	127	6/24 (25%)	43769.4 / 9.27	MOUSE	WNT-2B PROTEIN PRECURSOR (WNT-13)
4	127	6/24 (25%)	43770.5 / 9.32	HUMAN	WNT-2B PROTEIN PRECURSOR (WNT-13)
5	66.7	5/24 (20%)	49715.6 / 6.58	MOUSE	PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR BETA (PPAR-BETA) (PPAR-DELTA) (NUCLEAR HORMONE RECEPTOR 1) (NUC1)

Detailed Results

1. 10/24 matches (41%). 21892.1 Da, pI = 5.66. Acc. # P32119. HUMAN. PEROXIREDOXIN 2 (THIOREDOXIN PEROXIDASE 1) (THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE 1) (THIOL-SPECIFIC ANTIOXIDANT PROTEIN) (TSA) (PRP) (NATURAL KILLER CELL ENHANCING FACTOR B) (NKEF-B).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
789.3678	789.4107	-54.3291	151	157	(R)(L)
862.4543	862.5038	-57.4213	128	135	(R)(G)
924.4034	924.4427	-42.5395	120	127	(K)(G)
924.4034	924.4216	-19.6837	192	198	(K)(-)
1023.5040	1023.5363	-31.5430	111	119	(R)(T)
1179.6087	1179.6374	-24.3138	110	119	(R)(T)
1211.6493	1211.6748	-21.0581	140	150	(R)(S)
1734.9723	1734.9754	-1.8031	93	109	(K)(R)
1863.0675	1863.0704	-1.5615	92	109	(R)(R)
2713.4413	2713.3772	23.6236	68	91	(K)LGCEVLGVSVDSQFTHLAWINTPR(K)	1Cys-am
2841.5281	2841.4722	19.6721	67	91	(R)KLGCEVLGVSVDSQFTHLAWINTPR(K)	1Cys-am
2841.5281	2841.4722	19.6721	68	92	(K)LGCEVLGVSVDSQFTHLAWINTPRK(E)	1Cys-am

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The matched peptides cover 47% (94/198AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 41.7 Mean Error = -15.9404 ppm Data Tolerance = 56.4529 ppm Mean Missed Cleavages = 0.4

2. 6/24 matches (25%). 21778.8 Da, pI = 5.20. Acc. # Q61171. MOUSE. PEROXIREDOXIN 2 (THIOREDOXIN PEROXIDASE 1) (THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE 1) (THIOL-SPECIFIC ANTIOXIDANT PROTEIN) (TSA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
789.3678 789.4107 -54.3291 151 157 (R)(L)
924.4034 924.4216 -19.6837 192 198 (K)(-)
1023.5040 1023.5475 -42.5176 27 34 (K)(G)
1211.6493 1211.6748 -21.0581 140 150 (R)(S)
2713.4413 2713.3772 23.6236 68 91 (K)LGCEVLGVSVDSQFTHLAWINTPR(K) 1Cys-am
2841.5281 2841.4722 19.6721 67 91 (R)KLGCEVLGVSVDSQFTHLAWINTPR(K) 1Cys-am
2841.5281 2841.4722 19.6721 68 92 (K)LGCEVLGVSVDSQFTHLAWINTPRK(E) 1Cys-am

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The matched peptides cover 29% (59/198AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.0 Mean Error = -10.6601 ppm Data Tolerance = 63.9108 ppm Mean Missed Cleavages = 0.6

3. 6/24 matches (25%). 43769.4 Da, pI = 9.27. Acc. # O70283. MOUSE. WNT-2B PROTEIN PRECURSOR (WNT-13).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
862.4543 862.3947 69.1452 383 389 (K)(-)
924.4034 924.3919 12.3842 350 356 (R)(F)
1383.7022 1383.7055 -2.3233 370 381 (R)NTVDVHTCKAPK(K) 1Cys-am
1479.7610 1479.7201 27.6688 82 92 (R)(S)
1863.0675 1863.1869 -64.1156 37 53 (R)(V)
2713.4413 2713.2406 73.9685 347 366 (R)VTRVTQCECKFHWCCAVRCK(E) 2Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
862.4543	862.3947	69.1452	383	389	(K)(-)
924.4034	924.3919	12.3842	350	356	(R)(F)
1383.7022	1383.7055	-2.3233	370	381	(R)NTVDVHTCKAPK(K)	1Cys-am
1479.7610	1479.7201	27.6688	82	92	(R)(S)
1863.0675	1863.1869	-64.1156	37	53	(R)(V)
2713.4413	2713.2406	73.9685	347	366	(R)VTRVTQCECKFHWCCAVRCK(E)	2Cys-am

The matched peptides cover 17% (67/389AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.0 Mean Error = 19.4546 ppm Data Tolerance = 102.0456 ppm Mean Missed Cleavages = 0.8

4. 6/24 matches (25%). 43770.5 Da, pI = 9.32. Acc. # Q93097. HUMAN. WNT-2B PROTEIN PRECURSOR (WNT-13).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
862.4543 862.3947 69.1452 385 391 (K)(-)
924.4034 924.3919 12.3842 352 358 (R)(F)
1383.7022 1383.7055 -2.3233 372 383 (R)NTVDVHTCKAPK(K) 1Cys-am
1479.7610 1479.7201 27.6688 84 94 (R)(S)
1863.0675 1863.1869 -64.1156 39 55 (R)(V)
2713.4413 2713.2406 73.9685 349 368 (R)VTRVTQCECKFHWCCAVRCK(E) 2Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
862.4543	862.3947	69.1452	385	391	(K)(-)
924.4034	924.3919	12.3842	352	358	(R)(F)
1383.7022	1383.7055	-2.3233	372	383	(R)NTVDVHTCKAPK(K)	1Cys-am
1479.7610	1479.7201	27.6688	84	94	(R)(S)
1863.0675	1863.1869	-64.1156	39	55	(R)(V)
2713.4413	2713.2406	73.9685	349	368	(R)VTRVTQCECKFHWCCAVRCK(E)	2Cys-am

The matched peptides cover 17% (67/391AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.0 Mean Error = 19.4546 ppm Data Tolerance = 102.0456 ppm Mean Missed Cleavages = 0.8

5. 5/24 matches (20%). 49715.6 Da, pI = 6.58. Acc. # P35396. MOUSE. PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR BETA (PPAR-BETA) (PPAR-DELTA) (NUCLEAR HORMONE RECEPTOR 1) (NUC1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1194.6207 1194.5789 34.9828 248 257 (R)CQSTTVETVR(E) 1Cys-am
1756.9671 1756.8845 47.0197 180 193 (K)(K)
1863.0675 1862.9839 44.8729 283 299 (K)(D)
1885.0596 1884.9794 42.5146 180 194 (K)(K)
2713.4413 2713.2992 52.3564 80 102 (K)(M)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1194.6207	1194.5789	34.9828	248	257	(R)CQSTTVETVR(E)	1Cys-am
1756.9671	1756.8845	47.0197	180	193	(K)(K)
1863.0675	1862.9839	44.8729	283	299	(K)(D)
1885.0596	1884.9794	42.5146	180	194	(K)(K)
2713.4413	2713.2992	52.3564	80	102	(K)(M)

The matched peptides cover 14% (65/440AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.8 Mean Error = 44.3493 ppm Data Tolerance = 12.7521 ppm Mean Missed Cleavages = 1.2