MS-Fit Search Results

Press stop on your browser if you wish to abort this MS-Fit search prematurely.
Sample ID (comment): T004
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 933 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	75.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.99e+007	17/35 (48%)	48141.9 / 4.29	HUMAN	CALRETICULIN PRECURSOR (CRP55) (CALREGULIN) (HACBP) (ERP60) (52 KDA RIBONUCLEOPROTEIN AUTOANTIGEN RO/SS-A)
2	9.35e+005	14/35 (40%)	47994.9 / 4.33	MOUSE	CALRETICULIN PRECURSOR (CRP55) (CALREGULIN) (HACBP) (ERP60)
3	711	9/35 (25%)	83686.0 / 8.83	MOUSE	ECT2 PROTEIN (ECT2 ONCOGENE)
4	545	7/35 (20%)	98502.9 / 5.25	MOUSE	ROD CGMP-SPECIFIC 3',5'-CYCLIC PHOSPHODIESTERASE BETA-SUBUNIT (GMP-PDE BETA)
5	441	8/35 (22%)	62309.2 / 6.63	MOUSE	PYRUVATE KINASE, ISOZYMES R/L (L-PK)

Detailed Results

1. 17/35 matches (48%). 48141.9 Da, pI = 4.29. Acc. # P27797. HUMAN. CALRETICULIN PRECURSOR (CRP55) (CALREGULIN) (HACBP) (ERP60) (52 KDA RIBONUCLEOPROTEIN AUTOANTIGEN RO/SS-A).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
700.4010	700.4357	-49.5856	154	159	(K)(D)
737.3952	737.4198	-33.3556	49	55	(K)(F)
772.4042	772.4106	-8.2655	273	278	(K)(Q)
975.4954	975.4859	9.6842	65	73	(K)(F)
1147.6771	1147.6628	12.4607	143	151	(K)(G)
1219.7130	1219.7050	6.5155	88	98	(K)(H)
1410.6651	1410.6290	25.5754	25	36	(K)(W)
1490.7121	1490.6698	28.3956	99	111	(K)HEQNIDCGGGYVK(L)	1Cys-am
1607.8166	1607.7746	26.1329	74	87	(R)(G)
1800.8797	1800.8404	21.7815	208	222	(K)(A)
2391.2135	2391.0992	47.7959	186	206	(K)(K)
2519.3339	2519.1942	55.4712	186	207	(K)(I)
2760.4455	2760.3732	26.1927	186	209	(K)(D)
2760.4455	2760.2641	65.7394	225	248	(K)(K)
2870.5775	2870.4035	60.6296	163	185	(R)CKDDEFTHLYTLIVRPDNTYEVK(I)	1Cys-am
2960.4851	2960.2936	64.6607	249	272	(K)(G)
2976.4996	2976.2886	70.9239	249	272	(K)KPEDWDEEMDGEWEPPVIQNPEYK(G)	1Met-ox
3268.7078	3268.4962	64.7274	323	351	(K)(A)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 53% (224/417AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 48.6 Mean Error = 27.5267 ppm Data Tolerance = 69.2073 ppm Mean Missed Cleavages = 0.3

2. 14/35 matches (40%). 47994.9 Da, pI = 4.33. Acc. # P14211. MOUSE. CALRETICULIN PRECURSOR (CRP55) (CALREGULIN) (HACBP) (ERP60).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
700.4010 700.4357 -49.5856 154 159 (K)(D)
737.3952 737.4198 -33.3556 49 55 (K)(F)
772.4042 772.4106 -8.2655 273 278 (K)(Q)
975.4954 975.4859 9.6842 65 73 (K)(F)
1147.6771 1147.6628 12.4607 143 151 (K)(G)
1219.7130 1219.7050 6.5155 88 98 (K)(H)
1490.7121 1490.6698 28.3956 99 111 (K)HEQNIDCGGGYVK(L) 1Cys-am
2391.2135 2391.0992 47.7959 186 206 (K)(K)
2519.3339 2519.1942 55.4712 186 207 (K)(I)
2760.4455 2760.3732 26.1927 186 209 (K)(D)
2760.4455 2760.2641 65.7394 225 248 (K)(K)
2870.5775 2870.4035 60.6296 163 185 (R)CKDDEFTHLYTLIVRPDNTYEVK(I) 1Cys-am
2960.4851 2960.2936 64.6607 249 272 (K)(G)
2976.4996 2976.2886 70.9239 249 272 (K)KPEDWDEEMDGEWEPPVIQNPEYK(G) 1Met-ox
3268.7078 3268.4962 64.7274 323 351 (K)(A)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 44% (185/416AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 40.0 Mean Error = 28.1327 ppm Data Tolerance = 76.1798 ppm Mean Missed Cleavages = 0.3

3. 9/35 matches (25%). 83686.0 Da, pI = 8.83. Acc. # Q07139. MOUSE. ECT2 PROTEIN (ECT2 ONCOGENE).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
700.4010 700.3742 38.2740 727 732 (R)(S)
772.4042 772.4205 -21.0771 182 188 (K)(K)
1442.6422 1442.7313 -61.7732 133 144 (R)(D)
1800.8797 1800.9166 -20.4989 452 467 (K)AIGSLKEVMTHINEDK(R) 1Met-ox
2760.4455 2760.3231 44.3509 89 111 (R)NEFKVPPFQDCILSFLGFSDEEK(H) 1Cys-am
2827.5681 2827.7137 -51.4802 412 436 (R)(K)
2870.5775 2870.5034 25.8092 389 411 (K)CEKQKPRFHAFLKINQAKPECGR(Q) 2Cys-am
2876.4862 2876.2997 64.8503 71 92 (K)AWERRNEQCFCAAVDDFRNEFK(V) 2Cys-am
2976.4996 2976.4525 15.8255 470 495 (K)(S)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
700.4010	700.3742	38.2740	727	732	(R)(S)
772.4042	772.4205	-21.0771	182	188	(K)(K)
1442.6422	1442.7313	-61.7732	133	144	(R)(D)
1800.8797	1800.9166	-20.4989	452	467	(K)AIGSLKEVMTHINEDK(R)	1Met-ox
2760.4455	2760.3231	44.3509	89	111	(R)NEFKVPPFQDCILSFLGFSDEEK(H)	1Cys-am
2827.5681	2827.7137	-51.4802	412	436	(R)(K)
2870.5775	2870.5034	25.8092	389	411	(K)CEKQKPRFHAFLKINQAKPECGR(Q)	2Cys-am
2876.4862	2876.2997	64.8503	71	92	(K)AWERRNEQCFCAAVDDFRNEFK(V)	2Cys-am
2976.4996	2976.4525	15.8255	470	495	(K)(S)

The matched peptides cover 21% (156/738AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.7 Mean Error = 3.8090 ppm Data Tolerance = 88.7526 ppm Mean Missed Cleavages = 1.1

4. 7/35 matches (20%). 98502.9 Da, pI = 5.25. Acc. # P23440. MOUSE. ROD CGMP-SPECIFIC 3',5'-CYCLIC PHOSPHODIESTERASE BETA-SUBUNIT (GMP-PDE BETA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
772.4042 772.4430 -50.1618 800 805 (R)(E)
1475.7783 1475.7218 38.2799 222 232 (K)(T)
1475.7783 1475.7317 31.5738 446 457 (K)DIAQDMVLYHVR(C) 1Met-ox
2391.2135 2391.0709 59.6348 27 48 (K)LSPENVAGACEDGWLADCGSLR(D) 1Cys-am
2519.3339 2519.1659 66.7082 26 48 (K)KLSPENVAGACEDGWLADCGSLR(D) 1Cys-am
2519.3339 2519.2499 33.3610 441 460 (K)LENRKDIAQDMVLYHVRCDK(D) 1Met-ox
2870.5775 2870.3635 74.5690 707 731 (K)(V)
2960.4851 2960.4365 16.4023 1 26 (-)(L)
2976.4996 2976.4314 22.9245 1 26 (-)MSLSGEQVRSFLDGNPTFSHQYFGKK(L) 1Met-ox

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
772.4042	772.4430	-50.1618	800	805	(R)(E)
1475.7783	1475.7218	38.2799	222	232	(K)(T)
1475.7783	1475.7317	31.5738	446	457	(K)DIAQDMVLYHVR(C)	1Met-ox
2391.2135	2391.0709	59.6348	27	48	(K)LSPENVAGACEDGWLADCGSLR(D)	1Cys-am
2519.3339	2519.1659	66.7082	26	48	(K)KLSPENVAGACEDGWLADCGSLR(D)	1Cys-am
2519.3339	2519.2499	33.3610	441	460	(K)LENRKDIAQDMVLYHVRCDK(D)	1Met-ox
2870.5775	2870.3635	74.5690	707	731	(K)(V)
2960.4851	2960.4365	16.4023	1	26	(-)(L)
2976.4996	2976.4314	22.9245	1	26	(-)MSLSGEQVRSFLDGNPTFSHQYFGKK(L)	1Met-ox

The matched peptides cover 12% (110/856AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.0 Mean Error = 32.5880 ppm Data Tolerance = 73.8073 ppm Mean Missed Cleavages = 1.0

5. 8/35 matches (22%). 62309.2 Da, pI = 6.63. Acc. # P53657. MOUSE. PYRUVATE KINASE, ISOZYMES R/L (L-PK).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1219.7130 1219.6543 48.1449 466 476 (K)(T)
1475.7783 1475.8474 -46.8119 217 229 (R)(K)
1490.7121 1490.8187 -71.4939 104 116 (R)LKEMIKAGMNIAR(L) 1Met-ox
1800.8797 1800.9608 -45.0675 22 40 (K)(R)
2760.4455 2760.3701 27.3355 314 337 (K)(G)
2767.5585 2767.4102 53.5603 411 435 (K)(Q)
2960.4851 2960.5306 -15.3888 360 385 (R)CNLAGKPVVCATQMLESMITKARPTR(A) 2Cys-am
2976.4996 2976.5062 -2.2077 322 348 (K)(V)
2976.4996 2976.5255 -8.6959 360 385 (R)CNLAGKPVVCATQMLESMITKARPTR(A) 1Met-ox 2Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1219.7130	1219.6543	48.1449	466	476	(K)(T)
1475.7783	1475.8474	-46.8119	217	229	(R)(K)
1490.7121	1490.8187	-71.4939	104	116	(R)LKEMIKAGMNIAR(L)	1Met-ox
1800.8797	1800.9608	-45.0675	22	40	(K)(R)
2760.4455	2760.3701	27.3355	314	337	(K)(G)
2767.5585	2767.4102	53.5603	411	435	(K)(Q)
2960.4851	2960.5306	-15.3888	360	385	(R)CNLAGKPVVCATQMLESMITKARPTR(A)	2Cys-am
2976.4996	2976.5062	-2.2077	322	348	(K)(V)
2976.4996	2976.5255	-8.6959	360	385	(R)CNLAGKPVVCATQMLESMITKARPTR(A)	1Met-ox 2Cys-am

The matched peptides cover 24% (142/574AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 22.9 Mean Error = -6.7361 ppm Data Tolerance = 87.1108 ppm Mean Missed Cleavages = 1.2