MS-Fit Search Results

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Sample ID (comment): T005
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 728 entries (results displayed for top 5 matches).

Parameters Used in Search

4	50.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.27e+005	16/40 (40%)	92469.6 / 4.76	HUMAN	ENDOPLASMIN PRECURSOR (94 KDA GLUCOSE-REGULATED PROTEIN) (GRP94) (GP96 HOMOLOG) (TUMOR REJECTION ANTIGEN 1)
2	5.3e+004	15/40 (37%)	92476.5 / 4.74	MOUSE	ENDOPLASMIN PRECURSOR (ENDOPLASMIC RETICULUM PROTEIN 99) (94 KD GLUCOSE-REGULATED PROTEIN) (GRP94) (ERP99) (POLYMORPHIC TUMOR REJECTION ANTIGEN 1) (GP96)
3	1.8e+003	7/40 (17%)	80646.2 / 6.70	MOUSE	PROTEIN-TYROSINE PHOSPHATASE EPSILON PRECURSOR (R-PTP-EPSILON)
4	1.42e+003	9/40 (22%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)
5	423	9/40 (22%)	51599.2 / 8.11	MOUSE	NEURTURIN RECEPTOR ALPHA PRECURSOR (NTNR-ALPHA) (NRTNR-ALPHA) (TGF-BETA RELATED NEUROTROPHIC FACTOR RECEPTOR 2) (GDNF RECEPTOR BETA) (GDNFR-BETA)

Detailed Results

1. 16/40 matches (40%). 92469.6 Da, pI = 4.76. Acc. # P14625. HUMAN. ENDOPLASMIN PRECURSOR (94 KDA GLUCOSE-REGULATED PROTEIN) (GRP94) (GP96 HOMOLOG) (TUMOR REJECTION ANTIGEN 1).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
677.3557	677.3656	-14.6258	136	140	(K)IKCDK(E)	1Cys-am
876.4174	876.4579	-46.2378	684	690	(K)(H)
1031.4924	1031.4984	-5.8149	538	546	(K)(K)
1081.4931	1081.5431	-46.1878	76	84	(K)(M)
1150.5331	1150.5380	-4.3000	548	557	(K)(L)
1187.6428	1187.6788	-30.3477	385	395	(K)(G)
1187.6428	1187.7000	-48.1373	624	633	(K)(A)
1485.7169	1485.7549	-25.5583	435	448	(K)(E)
1515.6970	1515.7120	-9.9167	475	486	(K)(E)
1529.7792	1529.7746	3.0233	143	156	(K)NLLHVTDTGVGMTR(E)	1Met-ox
1529.7792	1529.7422	24.1791	535	547	(K)QDKIYFMAGSSRK(E)	pyroGlu 1Met-ox
1785.8375	1785.8983	-34.0547	52	67	(R)(E)
1878.9048	1878.9430	-20.3388	271	285	(K)(T)
1878.9048	1878.9312	-14.0134	609	623	(R)EAVEKEFEPLLNWMK(D)	1Met-ox
2260.0786	2260.0634	6.7122	512	530	(R)(M)
2370.0560	2370.0858	-12.5813	640	660	(R)LTESPCALVASQYGWSGNMER(I)	1Cys-am
2386.0608	2386.0808	-8.3448	640	660	(R)LTESPCALVASQYGWSGNMER(I)	1Met-ox 1Cys-am
2558.2803	2558.2866	-2.4401	329	348	(K)TVWDWELMNDIKPIWQRPSK(E)	1Met-ox
2728.3662	2728.3390	9.9789	44	67	(R)(E)
2728.3662	2728.2897	28.0318	640	663	(R)(A)

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The matched peptides cover 27% (222/803AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 40.0 Mean Error = -12.5487 ppm Data Tolerance = 43.2998 ppm Mean Missed Cleavages = 0.5

2. 15/40 matches (37%). 92476.5 Da, pI = 4.74. Acc. # P08113. MOUSE. ENDOPLASMIN PRECURSOR (ENDOPLASMIC RETICULUM PROTEIN 99) (94 KD GLUCOSE-REGULATED PROTEIN) (GRP94) (ERP99) (POLYMORPHIC TUMOR REJECTION ANTIGEN 1) (GP96).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
677.3557 677.3656 -14.6258 136 140 (K)IKCDK(E) 1Cys-am
876.4174 876.4579 -46.2378 684 690 (K)(H)
1031.4924 1031.4984 -5.8149 538 546 (K)(K)
1081.4931 1081.5431 -46.1878 76 84 (K)(M)
1150.5331 1150.5380 -4.3000 548 557 (K)(L)
1187.6428 1187.6788 -30.3477 385 395 (K)(G)
1187.6428 1187.7000 -48.1373 624 633 (K)(A)
1485.7169 1485.7549 -25.5583 435 448 (K)(E)
1529.7792 1529.7746 3.0233 143 156 (K)NLLHVTDTGVGMTR(E) 1Met-ox
1529.7792 1529.7276 33.7263 475 486 (K)(E)
1529.7792 1529.7422 24.1791 535 547 (K)QDKIYFMAGSSRK(E) pyroGlu 1Met-ox
1657.7655 1657.8226 -34.4156 474 486 (K)(E)
1785.8375 1785.8983 -34.0547 52 67 (R)(E)
1878.9048 1878.9430 -20.3388 271 285 (K)(T)
2370.0560 2370.0858 -12.5813 640 660 (R)LTESPCALVASQYGWSGNMER(I) 1Cys-am
2386.0608 2386.0808 -8.3448 640 660 (R)LTESPCALVASQYGWSGNMER(I) 1Met-ox 1Cys-am
2558.2803 2558.2866 -2.4401 329 348 (K)TVWDWELMNDIKPIWQRPSK(E) 1Met-ox
2728.3662 2728.3390 9.9789 44 67 (R)(E)
2728.3662 2728.2897 28.0318 640 663 (R)(A)

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The matched peptides cover 23% (189/802AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 37.5 Mean Error = -12.3392 ppm Data Tolerance = 49.7645 ppm Mean Missed Cleavages = 0.6

3. 7/40 matches (17%). 80646.2 Da, pI = 6.70. Acc. # P49446. MOUSE. PROTEIN-TYROSINE PHOSPHATASE EPSILON PRECURSOR (R-PTP-EPSILON).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1475.7186 1475.7099 5.9021 440 452 (K)(K)
1515.6970 1515.7088 -7.8357 276 287 (K)FCIHPQLPDSCK(A) 1Cys-am
1529.7792 1529.7245 35.7884 276 287 (K)FCIHPQLPDSCK(A) 2Cys-am
1657.7655 1657.8194 -32.5130 275 287 (R)KFCIHPQLPDSCK(A) 2Cys-am
2370.0560 2370.1375 -34.3695 666 683 (R)(V)
2386.0608 2386.1324 -29.9870 666 683 (R)LQRPHMVQTLEQYEFCYK(V) 1Met-ox
2511.1618 2511.1866 -9.8900 535 555 (K)CYQYWPTEGSVTHGDITIEIK(S) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1475.7186	1475.7099	5.9021	440	452	(K)(K)
1515.6970	1515.7088	-7.8357	276	287	(K)FCIHPQLPDSCK(A)	1Cys-am
1529.7792	1529.7245	35.7884	276	287	(K)FCIHPQLPDSCK(A)	2Cys-am
1657.7655	1657.8194	-32.5130	275	287	(R)KFCIHPQLPDSCK(A)	2Cys-am
2370.0560	2370.1375	-34.3695	666	683	(R)(V)
2386.0608	2386.1324	-29.9870	666	683	(R)LQRPHMVQTLEQYEFCYK(V)	1Met-ox
2511.1618	2511.1866	-9.8900	535	555	(K)CYQYWPTEGSVTHGDITIEIK(S)	1Cys-am

The matched peptides cover 9% (65/699AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.5 Mean Error = -10.4149 ppm Data Tolerance = 50.6829 ppm Mean Missed Cleavages = 0.3

4. 9/40 matches (22%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1015.4190 1015.4406 -21.2947 290 298 (K)DVDGAYMTK(V) 1Met-ox
1179.5556 1179.6010 -38.4485 377 386 (K)(H)
1187.6428 1187.6384 3.6753 433 443 (R)(L)
1383.6572 1383.6909 -24.3173 186 197 (K)(V)
1475.7186 1475.7858 -45.5456 200 211 (R)(W)
1475.7186 1475.7494 -20.8911 212 223 (K)(T)
1657.7655 1657.7934 -16.8176 13 29 (R)(R)
1716.8523 1716.8517 0.3930 418 432 (K)(G)
1993.9773 1993.9772 0.0340 224 239 (R)(R)
2260.0786 2260.0226 24.7994 252 269 (R)LDSELKNMQDMVEDYRNK(Y) 2Met-ox

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1015.4190	1015.4406	-21.2947	290	298	(K)DVDGAYMTK(V)	1Met-ox
1179.5556	1179.6010	-38.4485	377	386	(K)(H)
1187.6428	1187.6384	3.6753	433	443	(R)(L)
1383.6572	1383.6909	-24.3173	186	197	(K)(V)
1475.7186	1475.7858	-45.5456	200	211	(R)(W)
1475.7186	1475.7494	-20.8911	212	223	(K)(T)
1657.7655	1657.7934	-16.8176	13	29	(R)(R)
1716.8523	1716.8517	0.3930	418	432	(K)(G)
1993.9773	1993.9772	0.0340	224	239	(R)(R)
2260.0786	2260.0226	24.7994	252	269	(R)LDSELKNMQDMVEDYRNK(Y)	2Met-ox

The matched peptides cover 20% (132/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 22.5 Mean Error = -13.8413 ppm Data Tolerance = 42.3195 ppm Mean Missed Cleavages = 0.4

5. 9/40 matches (22%). 51599.2 Da, pI = 8.11. Acc. # O08842. MOUSE. NEURTURIN RECEPTOR ALPHA PRECURSOR (NTNR-ALPHA) (NRTNR-ALPHA) (TGF-BETA RELATED NEUROTROPHIC FACTOR RECEPTOR 2) (GDNF RECEPTOR BETA) (GDNFR-BETA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1015.4190 1015.4631 -43.4188 158 166 (K)(A)
1165.5537 1165.5312 19.2875 341 349 (K)DFTENPCLR(N) 1Cys-am
1657.7655 1657.7202 27.3628 327 340 (R)(D)
1707.7691 1707.8131 -25.7440 206 218 (R)(M)
1837.8764 1837.8880 -6.3062 266 280 (R)SRLADFHANCRASYR(T) 1Cys-am
2048.0179 2048.0354 -8.5598 203 218 (K)(M)
2370.0560 2370.1294 -30.9750 179 197 (R)SSYISICNREISPTERCNR(R) 2Cys-am
2511.1618 2511.1682 -2.5586 77 96 (K)ECQAALEVLQESPLYDCRCK(R) 3Cys-am
2728.3662 2728.3558 3.8176 247 267 (K)EKPNCLDLRSLCRTDHLCRSR(L) 3Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1015.4190	1015.4631	-43.4188	158	166	(K)(A)
1165.5537	1165.5312	19.2875	341	349	(K)DFTENPCLR(N)	1Cys-am
1657.7655	1657.7202	27.3628	327	340	(R)(D)
1707.7691	1707.8131	-25.7440	206	218	(R)(M)
1837.8764	1837.8880	-6.3062	266	280	(R)SRLADFHANCRASYR(T)	1Cys-am
2048.0179	2048.0354	-8.5598	203	218	(K)(M)
2370.0560	2370.1294	-30.9750	179	197	(R)SSYISICNREISPTERCNR(R)	2Cys-am
2511.1618	2511.1682	-2.5586	77	96	(K)ECQAALEVLQESPLYDCRCK(R)	3Cys-am
2728.3662	2728.3558	3.8176	247	267	(K)EKPNCLDLRSLCRTDHLCRSR(L)	3Cys-am

The matched peptides cover 26% (121/463AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 22.5 Mean Error = -7.4549 ppm Data Tolerance = 46.1633 ppm Mean Missed Cleavages = 1.3